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The map label for this gene is priA [H]

Identifier: 87200406

GI number: 87200406

Start: 2558451

End: 2560652

Strand: Direct

Name: priA [H]

Synonym: Saro_2393

Alternate gene names: 87200406

Gene position: 2558451-2560652 (Clockwise)

Preceding gene: 87200386

Following gene: 87200407

Centisome position: 71.83

GC content: 69.94

Gene sequence:

>2202_bases
ATGAGTGATGCACCCGCGCTCTTCCTAGACGGAAATTCGACCCGCGTCCGCTGTTTAATTTTCAACGCCGCCCTCGGCCC
GCTCGATTACCGGGTCCCCGCCGGGATGACGGTCGAGCCCGGCAGCGTGGTCATTGCGCCGCTCGGCCCGCGCCAGATCA
TCGGCGTGGTCTGGGATGCCGACCGGCTGCCCGCGACCGAGGTTCCGGAAGGCCGGTTGCGCCCCCTGCTCAAGGTCCTG
CCGGTTCCACCCCTGCCCGCTCCCTTGCGCCGCCTGATCGAATGGACCGCGGACTATTACCTTGCCCCCCTCGCGGCGGT
CGCGCGAATGGCCTTGTCGAGCAACGCGGCGCTCCAGGGCGGCAGCACCGTCACCGAATACCGCCTGACCGGCAAGGAGC
CGGGCCGCCTCACGCCGCAGCGCCTGATCGCGCTCGATCTCCTGCAGGACCAGCAGGCGACCATCCGCGAACTGTCCGAA
ATCGCCGGCGTTTCCGACGCCGTTCTGCGCGGCATGGTCAATGCCGGACTGCTCGAACCGGTCGTCGTCGATTCGGACCG
CCCCTATCCTGCGGCCGATCCCCACTTCGCCGCGCCCGACCTCTCGGAGCAGCAGGCCGAAGTCGCCGCCAGGTTCGTGG
CATCGGTGCGCGCGCGCGAATTTCACCCCTTCCTGCTCGATGGCGTCACCGGGTCGGGCAAGACCGAGACCTATTTCGAA
GGCGTCGCCGCCGCGCTCGAAGCCGGGCGACAGGTTCTCGTGCTGCTTCCCGAGATCGCGCTGACCGAAACCTTCCTCAA
ACGCTTCGAGGCCCGTTTCGGAGTGCCGCCCGTCACCTGGCATTCCTCGCTGAAGTCGTCCGAGCGGCGCCGCGCGTGGC
GCTCGGTGTCGTCAGGTGCCGCACAGGTCGTCGTCGGCGCACGCAGCGCCCTGTTCCTGCCGTTCGCCGATCTTGGCCTC
ATCGTGGTCGACGAAGCCCACGAAATCTCGTTCAAGCAGGACGACGGCGTGCGCTACAACGCCCGCGACGTGGCCGTCAT
GCGCGCCCACTTCGAGAAGATCCCGGTCATCCTCGCCAGCGCCACCCCAGCTCTCGAAAGCCTCCAGATGGCCGAGGCGG
GGCGCTACGAACGGGTGGTCCTGCCCGCCCGGTTCGGCGGCGCGACCATGCCCCACATCCAGGTCGTCGACCTGCGCGTG
GAACAGCCCGAACGCGGCCACTGGATCGCGCCGCCGCTGGTCAGGGCCCTGAAGGACCGTCTCGAAAAGGGCGAGCAATC
GCTGCTCTTCCTCAACCGCCGCGGCTATGCCCCGCTCACCTTGTGCCGCACCTGCGGCTACCGCTTCCAGTGCCCGAACT
GCTCGGCCTGGCTGGTCGAGCACCGCCTCTCGCGCCGCCTTGCCTGCCACCACTGCGGTCACGAGGTCCCGCCGCCCGAC
GCCTGCCCCGAATGCCACGAACCCGACTGCCTCGTCGCCTGCGGCCCAGGGGTAGAGCGCATTGCCGACGAAGTGGCCGA
GATCCTGCCCCACGCCCGCGTCGCGCTGGTCACCTCGGACACCCTGACCAGCCCCGCCAAGGCCGCCGAATTCGTCGAGA
TGGCCAGCAACAAGGCCATCGACGTGATCGTCGGCACGCAGCTCGTGACCAAGGGCTATCACTTCCCCGAACTCACCCTC
GTCGGCGTGATCGATGCCGACATGGGGCTCGAGGGCGGAGACCTGCGCGCGGCGGAGCGCACCTACCAGCAGGTCGCCCA
GGTCGCGGGCCGCGCCGGTCGCGGCGAAAAGCCGGGCGAAGTCCTGATCCAGACCCGCCACCCCGAAGCCCCCGTGATCG
AGGCTCTCGCCAGCGGCGACCGCGACGCCTTCTACGAGGCCGAGGCAGAGGCCCGCCGCGATGCCGGCGCGCCGCCCTTC
GGCCGCTGGGCCGCGATCATCGTGTCGAGCGAGGACGAGGCGGAAGCGCGCGATGCGGCGCGCGGCATCGGCGGCTCGGC
CCCGCGCCTCGACGACGTCCTCATCCTCGGCCCCGCGCCCGCCCCGCTCTCGCTCCTGCGGGGCCGCTACCGCTACCGCC
TGCTGATAAACGCCCGCCGCTCCACCGAACTCCAGCGCGTGATCCGCGAATGGCTCGATCCCCTGCGCTTCCCGCCGGGC
GTGCGCGTGGCGGTGGACATAGATCCCTACAGCTTCGTCTGA

Upstream 100 bases:

>100_bases
GGGAGCGACGCTGAATCGGGGAAGACAAGGCATTGATTTCAAGCGCATCCATCAGAGCTTCTCTTGGAAGTTCGGAAGCG
ACAGCTTGGGCTGAAGCTTC

Downstream 100 bases:

>100_bases
CCCCAAGGGGCAGGTTGCACCAGCCGCCCGGTCGGCCATTTCCGGACAATGTCCGCCCCCGCTCCCTCCACCCCCGTCAT
CGTCCCCATCCTCGGCGACC

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 733; Mature: 732

Protein sequence:

>733_residues
MSDAPALFLDGNSTRVRCLIFNAALGPLDYRVPAGMTVEPGSVVIAPLGPRQIIGVVWDADRLPATEVPEGRLRPLLKVL
PVPPLPAPLRRLIEWTADYYLAPLAAVARMALSSNAALQGGSTVTEYRLTGKEPGRLTPQRLIALDLLQDQQATIRELSE
IAGVSDAVLRGMVNAGLLEPVVVDSDRPYPAADPHFAAPDLSEQQAEVAARFVASVRAREFHPFLLDGVTGSGKTETYFE
GVAAALEAGRQVLVLLPEIALTETFLKRFEARFGVPPVTWHSSLKSSERRRAWRSVSSGAAQVVVGARSALFLPFADLGL
IVVDEAHEISFKQDDGVRYNARDVAVMRAHFEKIPVILASATPALESLQMAEAGRYERVVLPARFGGATMPHIQVVDLRV
EQPERGHWIAPPLVRALKDRLEKGEQSLLFLNRRGYAPLTLCRTCGYRFQCPNCSAWLVEHRLSRRLACHHCGHEVPPPD
ACPECHEPDCLVACGPGVERIADEVAEILPHARVALVTSDTLTSPAKAAEFVEMASNKAIDVIVGTQLVTKGYHFPELTL
VGVIDADMGLEGGDLRAAERTYQQVAQVAGRAGRGEKPGEVLIQTRHPEAPVIEALASGDRDAFYEAEAEARRDAGAPPF
GRWAAIIVSSEDEAEARDAARGIGGSAPRLDDVLILGPAPAPLSLLRGRYRYRLLINARRSTELQRVIREWLDPLRFPPG
VRVAVDIDPYSFV

Sequences:

>Translated_733_residues
MSDAPALFLDGNSTRVRCLIFNAALGPLDYRVPAGMTVEPGSVVIAPLGPRQIIGVVWDADRLPATEVPEGRLRPLLKVL
PVPPLPAPLRRLIEWTADYYLAPLAAVARMALSSNAALQGGSTVTEYRLTGKEPGRLTPQRLIALDLLQDQQATIRELSE
IAGVSDAVLRGMVNAGLLEPVVVDSDRPYPAADPHFAAPDLSEQQAEVAARFVASVRAREFHPFLLDGVTGSGKTETYFE
GVAAALEAGRQVLVLLPEIALTETFLKRFEARFGVPPVTWHSSLKSSERRRAWRSVSSGAAQVVVGARSALFLPFADLGL
IVVDEAHEISFKQDDGVRYNARDVAVMRAHFEKIPVILASATPALESLQMAEAGRYERVVLPARFGGATMPHIQVVDLRV
EQPERGHWIAPPLVRALKDRLEKGEQSLLFLNRRGYAPLTLCRTCGYRFQCPNCSAWLVEHRLSRRLACHHCGHEVPPPD
ACPECHEPDCLVACGPGVERIADEVAEILPHARVALVTSDTLTSPAKAAEFVEMASNKAIDVIVGTQLVTKGYHFPELTL
VGVIDADMGLEGGDLRAAERTYQQVAQVAGRAGRGEKPGEVLIQTRHPEAPVIEALASGDRDAFYEAEAEARRDAGAPPF
GRWAAIIVSSEDEAEARDAARGIGGSAPRLDDVLILGPAPAPLSLLRGRYRYRLLINARRSTELQRVIREWLDPLRFPPG
VRVAVDIDPYSFV
>Mature_732_residues
SDAPALFLDGNSTRVRCLIFNAALGPLDYRVPAGMTVEPGSVVIAPLGPRQIIGVVWDADRLPATEVPEGRLRPLLKVLP
VPPLPAPLRRLIEWTADYYLAPLAAVARMALSSNAALQGGSTVTEYRLTGKEPGRLTPQRLIALDLLQDQQATIRELSEI
AGVSDAVLRGMVNAGLLEPVVVDSDRPYPAADPHFAAPDLSEQQAEVAARFVASVRAREFHPFLLDGVTGSGKTETYFEG
VAAALEAGRQVLVLLPEIALTETFLKRFEARFGVPPVTWHSSLKSSERRRAWRSVSSGAAQVVVGARSALFLPFADLGLI
VVDEAHEISFKQDDGVRYNARDVAVMRAHFEKIPVILASATPALESLQMAEAGRYERVVLPARFGGATMPHIQVVDLRVE
QPERGHWIAPPLVRALKDRLEKGEQSLLFLNRRGYAPLTLCRTCGYRFQCPNCSAWLVEHRLSRRLACHHCGHEVPPPDA
CPECHEPDCLVACGPGVERIADEVAEILPHARVALVTSDTLTSPAKAAEFVEMASNKAIDVIVGTQLVTKGYHFPELTLV
GVIDADMGLEGGDLRAAERTYQQVAQVAGRAGRGEKPGEVLIQTRHPEAPVIEALASGDRDAFYEAEAEARRDAGAPPFG
RWAAIIVSSEDEAEARDAARGIGGSAPRLDDVLILGPAPAPLSLLRGRYRYRLLINARRSTELQRVIREWLDPLRFPPGV
RVAVDIDPYSFV

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=728, Percent_Identity=36.1263736263736, Blast_Score=405, Evalue=1e-114,
Organism=Escherichia coli, GI1787357, Length=168, Percent_Identity=30.952380952381, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD [H]

EC number: NA

Molecular weight: Translated: 79763; Mature: 79632

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS00690 DEAH_ATP_HELICASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDAPALFLDGNSTRVRCLIFNAALGPLDYRVPAGMTVEPGSVVIAPLGPRQIIGVVWDA
CCCCCEEEECCCCCEEEEEEEECCCCCCCEECCCCCEECCCCEEEEECCCCCEEEEEECC
DRLPATEVPEGRLRPLLKVLPVPPLPAPLRRLIEWTADYYLAPLAAVARMALSSNAALQG
CCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GSTVTEYRLTGKEPGRLTPQRLIALDLLQDQQATIRELSEIAGVSDAVLRGMVNAGLLEP
CCCEEEEEECCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCC
VVVDSDRPYPAADPHFAAPDLSEQQAEVAARFVASVRAREFHPFLLDGVTGSGKTETYFE
EEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHH
GVAAALEAGRQVLVLLPEIALTETFLKRFEARFGVPPVTWHSSLKSSERRRAWRSVSSGA
HHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCC
AQVVVGARSALFLPFADLGLIVVDEAHEISFKQDDGVRYNARDVAVMRAHFEKIPVILAS
EEEEEECCCEEEEECCCCCEEEEECCCCCCEECCCCCEECHHHHHHHHHHHHHCCEEEEC
ATPALESLQMAEAGRYERVVLPARFGGATMPHIQVVDLRVEQPERGHWIAPPLVRALKDR
CCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHH
LEKGEQSLLFLNRRGYAPLTLCRTCGYRFQCPNCSAWLVEHRLSRRLACHHCGHEVPPPD
HHCCCCEEEEEECCCCCHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
ACPECHEPDCLVACGPGVERIADEVAEILPHARVALVTSDTLTSPAKAAEFVEMASNKAI
CCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCE
DVIVGTQLVTKGYHFPELTLVGVIDADMGLEGGDLRAAERTYQQVAQVAGRAGRGEKPGE
EEEECCHHHHCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCC
VLIQTRHPEAPVIEALASGDRDAFYEAEAEARRDAGAPPFGRWAAIIVSSEDEAEARDAA
EEEEECCCCCHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEEECCCHHHHHHHH
RGIGGSAPRLDDVLILGPAPAPLSLLRGRYRYRLLINARRSTELQRVIREWLDPLRFPPG
HCCCCCCCCCCCEEEECCCCCCHHHHCCCEEEEEEEECHHHHHHHHHHHHHHHHHCCCCC
VRVAVDIDPYSFV
CEEEEECCCCCCC
>Mature Secondary Structure 
SDAPALFLDGNSTRVRCLIFNAALGPLDYRVPAGMTVEPGSVVIAPLGPRQIIGVVWDA
CCCCEEEECCCCCEEEEEEEECCCCCCCEECCCCCEECCCCEEEEECCCCCEEEEEECC
DRLPATEVPEGRLRPLLKVLPVPPLPAPLRRLIEWTADYYLAPLAAVARMALSSNAALQG
CCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GSTVTEYRLTGKEPGRLTPQRLIALDLLQDQQATIRELSEIAGVSDAVLRGMVNAGLLEP
CCCEEEEEECCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCC
VVVDSDRPYPAADPHFAAPDLSEQQAEVAARFVASVRAREFHPFLLDGVTGSGKTETYFE
EEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHH
GVAAALEAGRQVLVLLPEIALTETFLKRFEARFGVPPVTWHSSLKSSERRRAWRSVSSGA
HHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCC
AQVVVGARSALFLPFADLGLIVVDEAHEISFKQDDGVRYNARDVAVMRAHFEKIPVILAS
EEEEEECCCEEEEECCCCCEEEEECCCCCCEECCCCCEECHHHHHHHHHHHHHCCEEEEC
ATPALESLQMAEAGRYERVVLPARFGGATMPHIQVVDLRVEQPERGHWIAPPLVRALKDR
CCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHH
LEKGEQSLLFLNRRGYAPLTLCRTCGYRFQCPNCSAWLVEHRLSRRLACHHCGHEVPPPD
HHCCCCEEEEEECCCCCHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
ACPECHEPDCLVACGPGVERIADEVAEILPHARVALVTSDTLTSPAKAAEFVEMASNKAI
CCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCE
DVIVGTQLVTKGYHFPELTLVGVIDADMGLEGGDLRAAERTYQQVAQVAGRAGRGEKPGE
EEEECCHHHHCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCC
VLIQTRHPEAPVIEALASGDRDAFYEAEAEARRDAGAPPFGRWAAIIVSSEDEAEARDAA
EEEEECCCCCHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEEECCCHHHHHHHH
RGIGGSAPRLDDVLILGPAPAPLSLLRGRYRYRLLINARRSTELQRVIREWLDPLRFPPG
HCCCCCCCCCCCEEEECCCCCCHHHHCCCEEEEEEEECHHHHHHHHHHHHHHHHHCCCCC
VRVAVDIDPYSFV
CEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2861810 [H]