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The map label for this gene is fadA [H]

Identifier: 87200363

GI number: 87200363

Start: 2495287

End: 2496534

Strand: Direct

Name: fadA [H]

Synonym: Saro_2349

Alternate gene names: 87200363

Gene position: 2495287-2496534 (Clockwise)

Preceding gene: 87200356

Following gene: 87200365

Centisome position: 70.06

GC content: 66.59

Gene sequence:

>1248_bases
ATGCCCGAAGCCTATATCATCGACGCCGTCCGTACGCCGCGCGGGATCGGCAAGCCCGGCAAGGGCGCCCTTTCGCACCT
GCACCCGCAGCATCTTGCCGCGACGGTGCTCAAGGCGCTGAAGGACCGCAACGACCTGGACACCTCGACCGTCGACGACA
TCATCTGGTCGACCTCGTCGCAGGTGGGCAAGCAGGGCGGCGATCTTGGCCGCATGGCAGCGCTGGCGGCAGGGTACGAC
ATCTCGGCCAGCGGCACGACGCTCGACCGGTTCTGCGGCGGCGGCATCACTTCGGTGAACCTTGCCTGCGCCTCGGTCAT
GTCGGGCATGGAAGATTGCGTGATCGCCGGCGGCACCGAGATGATGAGCTTCACCGCCGCCCACGGCGCGGAGCTGGCCA
ATGCCGGGATCAAGCCGCTGGGCATGGGTTCGGGCAACATGGAACTGGATGCCCTGCACCCGCAGTCGCACCAGGGCGTG
TGCGGCGATGCCATTGCCGCGCGCGAGGGGATCGACCGCATGGCGCTCGACGCGCTCGCCCTCGTAAGCCAGGAACGCGC
CGACCGCGCGATCCGCGAGGGGCGCTTCGACAAGTCGGTGGTCACCGTCTACAACCCCGACGGCACGGTGGCGCTCGACC
ACGAGGAATTCCCGCGTCCGGAAACGACGATGGAAGGCCTGTCCTCGCTCAAGGCAAGCTTCGACGGAATTGCCGACTTC
GACCTTGGCGGCACCACGTTCCGCAAGCAGATCCAGCGCCGCTATCCGGAACTGGAGTGGAAGGGCGTGCATCACGCGGG
CAACTCCTCCGGCGTGGTCGACGGTGCGGCGGCGCTGCTGATCGTTTCACCCGCCTATGCCGAGAAGCATGGCCTGAAGC
CGCGCGCGCGCATCGTTGCCTATGCCAACCAGGGCGATGACCCGACGCTCATGCTGAACGCTCCGGTGCCGGCGGCAAGG
AAGGTCCTCGACAAGGCTGGCCTTTCGAAGGACGACATCGACGTCTGGGAAATCAACGAGGCCTTCGCGGTCGTGGCCGA
GAAGTTCATCCGCGATCTCGAACTGGACCGCGACAAGGTGAACATCAACGGCGGCGCGATGGCATTGGGCCATCCGATCG
GCGCGACGGGATCGATCCTGATCGGCACCGCGCTCGACGAACTGGAGCGGTCGGGCGGGCGCTATGGCCTGGTCACGATG
TGCGCGGCAGGCGGCATGGCACCGGCGATCATCATCGAACGCATCTGA

Upstream 100 bases:

>100_bases
GCACGCGCCTGCTGTCGAGCCGTCGGAATCGGCTTGCACATGCACAAATGGCACGTTAATCGATCGATTAAAGTCAAAGA
TCGCAACCGGAGAGCCTGAG

Downstream 100 bases:

>100_bases
ACGGGCCTTGGCGCCACGCAACGGAAAAAGGCTCCCCCGCGACGGGGAGCCTTTTTTCGTAAGGGCGCAAGTTCAGTCGG
CCGCGACGATGGGACCCGGC

Product: acetyl-CoA acetyltransferase

Products: CoA; 3-oxoacyl-CoA

Alternate protein names: NA

Number of amino acids: Translated: 415; Mature: 414

Protein sequence:

>415_residues
MPEAYIIDAVRTPRGIGKPGKGALSHLHPQHLAATVLKALKDRNDLDTSTVDDIIWSTSSQVGKQGGDLGRMAALAAGYD
ISASGTTLDRFCGGGITSVNLACASVMSGMEDCVIAGGTEMMSFTAAHGAELANAGIKPLGMGSGNMELDALHPQSHQGV
CGDAIAAREGIDRMALDALALVSQERADRAIREGRFDKSVVTVYNPDGTVALDHEEFPRPETTMEGLSSLKASFDGIADF
DLGGTTFRKQIQRRYPELEWKGVHHAGNSSGVVDGAAALLIVSPAYAEKHGLKPRARIVAYANQGDDPTLMLNAPVPAAR
KVLDKAGLSKDDIDVWEINEAFAVVAEKFIRDLELDRDKVNINGGAMALGHPIGATGSILIGTALDELERSGGRYGLVTM
CAAGGMAPAIIIERI

Sequences:

>Translated_415_residues
MPEAYIIDAVRTPRGIGKPGKGALSHLHPQHLAATVLKALKDRNDLDTSTVDDIIWSTSSQVGKQGGDLGRMAALAAGYD
ISASGTTLDRFCGGGITSVNLACASVMSGMEDCVIAGGTEMMSFTAAHGAELANAGIKPLGMGSGNMELDALHPQSHQGV
CGDAIAAREGIDRMALDALALVSQERADRAIREGRFDKSVVTVYNPDGTVALDHEEFPRPETTMEGLSSLKASFDGIADF
DLGGTTFRKQIQRRYPELEWKGVHHAGNSSGVVDGAAALLIVSPAYAEKHGLKPRARIVAYANQGDDPTLMLNAPVPAAR
KVLDKAGLSKDDIDVWEINEAFAVVAEKFIRDLELDRDKVNINGGAMALGHPIGATGSILIGTALDELERSGGRYGLVTM
CAAGGMAPAIIIERI
>Mature_414_residues
PEAYIIDAVRTPRGIGKPGKGALSHLHPQHLAATVLKALKDRNDLDTSTVDDIIWSTSSQVGKQGGDLGRMAALAAGYDI
SASGTTLDRFCGGGITSVNLACASVMSGMEDCVIAGGTEMMSFTAAHGAELANAGIKPLGMGSGNMELDALHPQSHQGVC
GDAIAAREGIDRMALDALALVSQERADRAIREGRFDKSVVTVYNPDGTVALDHEEFPRPETTMEGLSSLKASFDGIADFD
LGGTTFRKQIQRRYPELEWKGVHHAGNSSGVVDGAAALLIVSPAYAEKHGLKPRARIVAYANQGDDPTLMLNAPVPAARK
VLDKAGLSKDDIDVWEINEAFAVVAEKFIRDLELDRDKVNINGGAMALGHPIGATGSILIGTALDELERSGGRYGLVTMC
AAGGMAPAIIIERI

Specific function: Thiolytic Cleavage Of Beta-Ketoadipyl-CoA To Succinate And Acetyl-CoA (By Similarity). [C]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI148539872, Length=423, Percent_Identity=34.2789598108747, Blast_Score=209, Evalue=3e-54,
Organism=Homo sapiens, GI167614485, Length=418, Percent_Identity=33.0143540669856, Blast_Score=190, Evalue=2e-48,
Organism=Homo sapiens, GI4557237, Length=427, Percent_Identity=32.7868852459016, Blast_Score=187, Evalue=2e-47,
Organism=Homo sapiens, GI4501853, Length=407, Percent_Identity=34.6437346437346, Blast_Score=176, Evalue=3e-44,
Organism=Homo sapiens, GI4504327, Length=445, Percent_Identity=29.438202247191, Blast_Score=142, Evalue=5e-34,
Organism=Homo sapiens, GI194353979, Length=89, Percent_Identity=50.561797752809, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI1787663, Length=428, Percent_Identity=40.8878504672897, Blast_Score=249, Evalue=2e-67,
Organism=Escherichia coli, GI48994986, Length=423, Percent_Identity=34.0425531914894, Blast_Score=221, Evalue=9e-59,
Organism=Escherichia coli, GI87082165, Length=423, Percent_Identity=36.6430260047281, Blast_Score=211, Evalue=7e-56,
Organism=Escherichia coli, GI1788554, Length=424, Percent_Identity=36.0849056603774, Blast_Score=209, Evalue=3e-55,
Organism=Escherichia coli, GI1788683, Length=419, Percent_Identity=26.7303102625298, Blast_Score=108, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI133906874, Length=418, Percent_Identity=31.8181818181818, Blast_Score=179, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI25147385, Length=418, Percent_Identity=30.622009569378, Blast_Score=172, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI17535921, Length=421, Percent_Identity=28.0285035629454, Blast_Score=147, Evalue=9e-36,
Organism=Caenorhabditis elegans, GI17551802, Length=452, Percent_Identity=28.9823008849558, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17535917, Length=417, Percent_Identity=27.5779376498801, Blast_Score=123, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6322031, Length=418, Percent_Identity=31.3397129186603, Blast_Score=165, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6325229, Length=427, Percent_Identity=32.0843091334895, Blast_Score=157, Evalue=3e-39,
Organism=Drosophila melanogaster, GI24655093, Length=424, Percent_Identity=35.8490566037736, Blast_Score=238, Evalue=4e-63,
Organism=Drosophila melanogaster, GI17648125, Length=431, Percent_Identity=31.554524361949, Blast_Score=168, Evalue=7e-42,
Organism=Drosophila melanogaster, GI24640423, Length=425, Percent_Identity=30.3529411764706, Blast_Score=167, Evalue=8e-42,
Organism=Drosophila melanogaster, GI17137578, Length=448, Percent_Identity=28.3482142857143, Blast_Score=123, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: 2.3.1.16

Molecular weight: Translated: 43626; Mature: 43495

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEAYIIDAVRTPRGIGKPGKGALSHLHPQHLAATVLKALKDRNDLDTSTVDDIIWSTSS
CCCCEEEECCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCH
QVGKQGGDLGRMAALAAGYDISASGTTLDRFCGGGITSVNLACASVMSGMEDCVIAGGTE
HHHHCCCCHHHHHHHHCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHCCHHHEEEECCHH
MMSFTAAHGAELANAGIKPLGMGSGNMELDALHPQSHQGVCGDAIAAREGIDRMALDALA
HHHHHHHHHHHHHHCCCCEEECCCCCEEEEEECCCCCCCCCHHHHHHHHCHHHHHHHHHH
LVSQERADRAIREGRFDKSVVTVYNPDGTVALDHEEFPRPETTMEGLSSLKASFDGIADF
HHHHHHHHHHHHHCCCCCCEEEEECCCCCEEECHHHCCCCHHHHHHHHHHHHHCCCCCCC
DLGGTTFRKQIQRRYPELEWKGVHHAGNSSGVVDGAAALLIVSPAYAEKHGLKPRARIVA
CCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHCCEEEEEECCHHHHHHCCCCCCEEEE
YANQGDDPTLMLNAPVPAARKVLDKAGLSKDDIDVWEINEAFAVVAEKFIRDLELDRDKV
EECCCCCCEEEEECCCHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCEE
NINGGAMALGHPIGATGSILIGTALDELERSGGRYGLVTMCAAGGMAPAIIIERI
EECCCEEEECCCCCCCCCEEEHHHHHHHHHCCCCEEHHHHHHCCCCCHHHEEECC
>Mature Secondary Structure 
PEAYIIDAVRTPRGIGKPGKGALSHLHPQHLAATVLKALKDRNDLDTSTVDDIIWSTSS
CCCEEEECCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCH
QVGKQGGDLGRMAALAAGYDISASGTTLDRFCGGGITSVNLACASVMSGMEDCVIAGGTE
HHHHCCCCHHHHHHHHCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHCCHHHEEEECCHH
MMSFTAAHGAELANAGIKPLGMGSGNMELDALHPQSHQGVCGDAIAAREGIDRMALDALA
HHHHHHHHHHHHHHCCCCEEECCCCCEEEEEECCCCCCCCCHHHHHHHHCHHHHHHHHHH
LVSQERADRAIREGRFDKSVVTVYNPDGTVALDHEEFPRPETTMEGLSSLKASFDGIADF
HHHHHHHHHHHHHCCCCCCEEEEECCCCCEEECHHHCCCCHHHHHHHHHHHHHCCCCCCC
DLGGTTFRKQIQRRYPELEWKGVHHAGNSSGVVDGAAALLIVSPAYAEKHGLKPRARIVA
CCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHCCEEEEEECCHHHHHHCCCCCCEEEE
YANQGDDPTLMLNAPVPAARKVLDKAGLSKDDIDVWEINEAFAVVAEKFIRDLELDRDKV
EECCCCCCEEEEECCCHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCEE
NINGGAMALGHPIGATGSILIGTALDELERSGGRYGLVTMCAAGGMAPAIIIERI
EECCCEEEECCCCCCCCCEEEHHHHHHHHHCCCCEEHHHHHHCCCCCHHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: acyl-CoA; acetyl-CoA

Specific reaction: acyl-CoA + acetyl-CoA = CoA + 3-oxoacyl-CoA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230 [H]