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The map label for this gene is asnB [H]

Identifier: 87200341

GI number: 87200341

Start: 2471447

End: 2473336

Strand: Reverse

Name: asnB [H]

Synonym: Saro_2327

Alternate gene names: 87200341

Gene position: 2473336-2471447 (Counterclockwise)

Preceding gene: 87200342

Following gene: 87200340

Centisome position: 69.44

GC content: 67.88

Gene sequence:

>1890_bases
ATGTGCGGAATTGCCGGAATCGTCCATACCGAGGGCCCGAAGCCCGTCGACCCAGACCGTGTCCGCCGCATGTGCGATGC
CATCGCGCATCGCGGGCCCGACGGGGAAGGCGTGTGGACCGCGCCCGGCATCGGCCTGGGGCACCGCCGTCTCTCCATCA
TCGACCTGGCGGGATCGCCCCAGCCGATGAAGGCCGCCGAAGGCGACGCGGTCATCGTCTTCAACGGCGAAATCTACAAC
TTCAGGGACTTGCGCAAGGAACTCCAGGCCCTGGGCGCACAGTTCCGCACCGATGGCGATACCGAGGTGATCCTGCAGGC
CTGGCGCCAATGGGGTACGGCGTGCCTGCCGCGCCTCCACGGCATGTTCGCCTTTGCCCTTTACGACCTCCGCCAACGCA
CGACCCTGCTCGCGCGGGACCGCTTGGGCGTGAAGCCGCTGTTCACGGCGCGCCTAGCCGATGGCGCGGTTATCTTCGGC
TCCGAACTCAAGGCGCTGCTCGCGCACCCGTCGCTGCGGCGCGAAATCGATCCGCTGGCGGTCGAGGATTATCTCGCCTG
GGGCTATGTCCCGGACCACCGATCGATGCTCAAGGGGGTGGAGAAGCTGCCCGCCGGGCACTACCAGCTCCTGCGCCATG
ATGCCCCGCCGGCCGCGCCGGTGCAGTGGTGGGACGTCTCCTTCGCCGACCGCGCCAATGGTAGCGCCGAAGACCTTGGC
GCGCACCTCCTGCACCACATGCGCGACGCGGTGACCTCGCGCATGGTCGCCGACGTGCCGCTTGGCGCCTTCCTCTCCGG
CGGGGTCGACAGCTCCAGTGTGGTCGCGCTCATGGCCGAGGCGAGCGCCACGCCGGTCAAGACCTGTTCGATCGGTTTCG
ACGTCGCCGCGCTCGACGAGACGGCCTATGCCGACCGGATTGCCGCGCGCTTCGCCACCGAACACCGCAAGCGCCTGGTT
TCTCCAGATGATTTCTCGCTGGTGGACAGGCTGGCGGGCATGTTCGACGAACCCTTCGCCGATGCTTCCGCGCTACCCAC
CTTCCGCGTGTGCGAGCTAGCGCGCGAACATGTGACCGTCGCACTCTCGGGCGACGGCGCGGACGAGGCCTTTGCCGGTT
ACCGCCGCCAGACCTTCCAGCACCGCGAGGAACAGGCGCGGGCGCTGCTGCCTTCCGCCTTGCGCAAGCCGCTGTTCGGC
ACGCTTGGCCGGTTATGGCCCAAGGCGGACTGGGCGCCGCGCCCCTTGCGCGCGAAGACGACGCTGCTCAGCCTCGCGGC
TTCCGGGGAGGAGGGCTATGCCCGCGCGCTTTCGGTCACGTCGCCCGAGCTGCGAGAGACGCTTTACACGCCGGACTTCA
AGCGACTGCGCGGCGACTACCGCGCCGAACAGCCACTCATCGAGTTGATGCGGAAGGCGCCGGCCCGCTCGGGACTGGAT
CGCGCGCAGTATGCCGATCTCAAGTTCTACCTGCCAGGCGACATCCTGACCAAGGTCGATCGCACCAGCATGGCCGTCGG
CCTTGAAGCGCGCGAGCCGTTGCTGGATCATCGCCTGGTGGAATTCGGCGCAAGCCTGCCGGAACGGCTGCGGGTGCGCG
GTGCGACCGGCAAATGGCTGATAAAGCAGACAATGCGCCGCTATCTGCCGGAAGATGTGCTGTTCAGGCCGAAGATGGGT
TTCGTCACGCCGCTGGCCCAGTGGCTGCGCGGTCCGCTTGCGCACGAGGCACGGGCGCTGGCGTCGGACGGCCTGCTCGC
CCGCACCGGCTGGCTGGACGCAGCCCGTATCGACGCCCTGGCCGATGCGCACGTGTCGGGGAGGGCGGATCACAGCCGGC
TCATCTGGCAGTTGCTGATGCTCCGGAAATCCTTGCAGCTTCTTGGTTGA

Upstream 100 bases:

>100_bases
CCAGCCGTCGGCCGAACAGTCCATGCGCGCCTTCCTGTCGGCCATCGGTCCGGTCGATGCGTGGATGGACCGCGCTGCCT
TGCCCCGCTAGGCCCCTCGC

Downstream 100 bases:

>100_bases
TTGCCAATTAATCATGACATTTCGGTAAGACGTTCTTCACCATTGCGGTCTAGTCAGGCTGTGAAACAACCGCCGATGGT
CCATGCTGAAGAAACTGCGC

Product: asparagine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 629; Mature: 629

Protein sequence:

>629_residues
MCGIAGIVHTEGPKPVDPDRVRRMCDAIAHRGPDGEGVWTAPGIGLGHRRLSIIDLAGSPQPMKAAEGDAVIVFNGEIYN
FRDLRKELQALGAQFRTDGDTEVILQAWRQWGTACLPRLHGMFAFALYDLRQRTTLLARDRLGVKPLFTARLADGAVIFG
SELKALLAHPSLRREIDPLAVEDYLAWGYVPDHRSMLKGVEKLPAGHYQLLRHDAPPAAPVQWWDVSFADRANGSAEDLG
AHLLHHMRDAVTSRMVADVPLGAFLSGGVDSSSVVALMAEASATPVKTCSIGFDVAALDETAYADRIAARFATEHRKRLV
SPDDFSLVDRLAGMFDEPFADASALPTFRVCELAREHVTVALSGDGADEAFAGYRRQTFQHREEQARALLPSALRKPLFG
TLGRLWPKADWAPRPLRAKTTLLSLAASGEEGYARALSVTSPELRETLYTPDFKRLRGDYRAEQPLIELMRKAPARSGLD
RAQYADLKFYLPGDILTKVDRTSMAVGLEAREPLLDHRLVEFGASLPERLRVRGATGKWLIKQTMRRYLPEDVLFRPKMG
FVTPLAQWLRGPLAHEARALASDGLLARTGWLDAARIDALADAHVSGRADHSRLIWQLLMLRKSLQLLG

Sequences:

>Translated_629_residues
MCGIAGIVHTEGPKPVDPDRVRRMCDAIAHRGPDGEGVWTAPGIGLGHRRLSIIDLAGSPQPMKAAEGDAVIVFNGEIYN
FRDLRKELQALGAQFRTDGDTEVILQAWRQWGTACLPRLHGMFAFALYDLRQRTTLLARDRLGVKPLFTARLADGAVIFG
SELKALLAHPSLRREIDPLAVEDYLAWGYVPDHRSMLKGVEKLPAGHYQLLRHDAPPAAPVQWWDVSFADRANGSAEDLG
AHLLHHMRDAVTSRMVADVPLGAFLSGGVDSSSVVALMAEASATPVKTCSIGFDVAALDETAYADRIAARFATEHRKRLV
SPDDFSLVDRLAGMFDEPFADASALPTFRVCELAREHVTVALSGDGADEAFAGYRRQTFQHREEQARALLPSALRKPLFG
TLGRLWPKADWAPRPLRAKTTLLSLAASGEEGYARALSVTSPELRETLYTPDFKRLRGDYRAEQPLIELMRKAPARSGLD
RAQYADLKFYLPGDILTKVDRTSMAVGLEAREPLLDHRLVEFGASLPERLRVRGATGKWLIKQTMRRYLPEDVLFRPKMG
FVTPLAQWLRGPLAHEARALASDGLLARTGWLDAARIDALADAHVSGRADHSRLIWQLLMLRKSLQLLG
>Mature_629_residues
MCGIAGIVHTEGPKPVDPDRVRRMCDAIAHRGPDGEGVWTAPGIGLGHRRLSIIDLAGSPQPMKAAEGDAVIVFNGEIYN
FRDLRKELQALGAQFRTDGDTEVILQAWRQWGTACLPRLHGMFAFALYDLRQRTTLLARDRLGVKPLFTARLADGAVIFG
SELKALLAHPSLRREIDPLAVEDYLAWGYVPDHRSMLKGVEKLPAGHYQLLRHDAPPAAPVQWWDVSFADRANGSAEDLG
AHLLHHMRDAVTSRMVADVPLGAFLSGGVDSSSVVALMAEASATPVKTCSIGFDVAALDETAYADRIAARFATEHRKRLV
SPDDFSLVDRLAGMFDEPFADASALPTFRVCELAREHVTVALSGDGADEAFAGYRRQTFQHREEQARALLPSALRKPLFG
TLGRLWPKADWAPRPLRAKTTLLSLAASGEEGYARALSVTSPELRETLYTPDFKRLRGDYRAEQPLIELMRKAPARSGLD
RAQYADLKFYLPGDILTKVDRTSMAVGLEAREPLLDHRLVEFGASLPERLRVRGATGKWLIKQTMRRYLPEDVLFRPKMG
FVTPLAQWLRGPLAHEARALASDGLLARTGWLDAARIDALADAHVSGRADHSRLIWQLLMLRKSLQLLG

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=395, Percent_Identity=26.8354430379747, Blast_Score=110, Evalue=4e-24,
Organism=Homo sapiens, GI168229252, Length=395, Percent_Identity=26.8354430379747, Blast_Score=110, Evalue=4e-24,
Organism=Homo sapiens, GI168229250, Length=395, Percent_Identity=26.8354430379747, Blast_Score=110, Evalue=4e-24,
Organism=Homo sapiens, GI296010848, Length=368, Percent_Identity=27.445652173913, Blast_Score=110, Evalue=5e-24,
Organism=Homo sapiens, GI296010852, Length=303, Percent_Identity=26.7326732673267, Blast_Score=80, Evalue=7e-15,
Organism=Homo sapiens, GI296010850, Length=303, Percent_Identity=26.7326732673267, Blast_Score=80, Evalue=7e-15,
Organism=Escherichia coli, GI1786889, Length=420, Percent_Identity=27.8571428571429, Blast_Score=139, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI71993933, Length=393, Percent_Identity=30.5343511450382, Blast_Score=124, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI25147557, Length=393, Percent_Identity=30.5343511450382, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI25147560, Length=354, Percent_Identity=31.0734463276836, Blast_Score=113, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6321563, Length=410, Percent_Identity=25.609756097561, Blast_Score=110, Evalue=9e-25,
Organism=Saccharomyces cerevisiae, GI6325403, Length=410, Percent_Identity=26.8292682926829, Blast_Score=106, Evalue=1e-23,
Organism=Drosophila melanogaster, GI45553209, Length=400, Percent_Identity=29.25, Blast_Score=133, Evalue=4e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 69476; Mature: 69476

Theoretical pI: Translated: 8.67; Mature: 8.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIAGIVHTEGPKPVDPDRVRRMCDAIAHRGPDGEGVWTAPGIGLGHRRLSIIDLAGSP
CCCCCCEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCEEEEEEECCCC
QPMKAAEGDAVIVFNGEIYNFRDLRKELQALGAQFRTDGDTEVILQAWRQWGTACLPRLH
CCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
GMFAFALYDLRQRTTLLARDRLGVKPLFTARLADGAVIFGSELKALLAHPSLRREIDPLA
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCEEEEHHHHHHHHHCCHHHHCCCCHH
VEDYLAWGYVPDHRSMLKGVEKLPAGHYQLLRHDAPPAAPVQWWDVSFADRANGSAEDLG
HHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHCCCCCCCCCEEECCCHHHCCCCCHHHHH
AHLLHHMRDAVTSRMVADVPLGAFLSGGVDSSSVVALMAEASATPVKTCSIGFDVAALDE
HHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHCCCCCCEEEECCCCHHHHCH
TAYADRIAARFATEHRKRLVSPDDFSLVDRLAGMFDEPFADASALPTFRVCELAREHVTV
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCEEE
ALSGDGADEAFAGYRRQTFQHREEQARALLPSALRKPLFGTLGRLWPKADWAPRPLRAKT
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
TLLSLAASGEEGYARALSVTSPELRETLYTPDFKRLRGDYRAEQPLIELMRKAPARSGLD
HHHHHHCCCCCHHHHEEECCCHHHHHHHCCCCHHHHHCHHCCCCHHHHHHHHCCCCCCCC
RAQYADLKFYLPGDILTKVDRTSMAVGLEAREPLLDHRLVEFGASLPERLRVRGATGKWL
HHHHCCEEEECCHHHHHHHHHHHHHCCCCHHCHHHHHHHHHHCCCCCHHHEECCCCCHHH
IKQTMRRYLPEDVLFRPKMGFVTPLAQWLRGPLAHEARALASDGLLARTGWLDAARIDAL
HHHHHHHHCCHHHEECCCCCHHHHHHHHHHCHHHHHHHHHHCCCCEEECCCCCHHHHHHH
ADAHVSGRADHSRLIWQLLMLRKSLQLLG
HHCCCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MCGIAGIVHTEGPKPVDPDRVRRMCDAIAHRGPDGEGVWTAPGIGLGHRRLSIIDLAGSP
CCCCCCEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCEEEEEEECCCC
QPMKAAEGDAVIVFNGEIYNFRDLRKELQALGAQFRTDGDTEVILQAWRQWGTACLPRLH
CCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
GMFAFALYDLRQRTTLLARDRLGVKPLFTARLADGAVIFGSELKALLAHPSLRREIDPLA
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCEEEEHHHHHHHHHCCHHHHCCCCHH
VEDYLAWGYVPDHRSMLKGVEKLPAGHYQLLRHDAPPAAPVQWWDVSFADRANGSAEDLG
HHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHCCCCCCCCCEEECCCHHHCCCCCHHHHH
AHLLHHMRDAVTSRMVADVPLGAFLSGGVDSSSVVALMAEASATPVKTCSIGFDVAALDE
HHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHCCCCCCEEEECCCCHHHHCH
TAYADRIAARFATEHRKRLVSPDDFSLVDRLAGMFDEPFADASALPTFRVCELAREHVTV
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCEEE
ALSGDGADEAFAGYRRQTFQHREEQARALLPSALRKPLFGTLGRLWPKADWAPRPLRAKT
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
TLLSLAASGEEGYARALSVTSPELRETLYTPDFKRLRGDYRAEQPLIELMRKAPARSGLD
HHHHHHCCCCCHHHHEEECCCHHHHHHHCCCCHHHHHCHHCCCCHHHHHHHHCCCCCCCC
RAQYADLKFYLPGDILTKVDRTSMAVGLEAREPLLDHRLVEFGASLPERLRVRGATGKWL
HHHHCCEEEECCHHHHHHHHHHHHHCCCCHHCHHHHHHHHHHCCCCCHHHEECCCCCHHH
IKQTMRRYLPEDVLFRPKMGFVTPLAQWLRGPLAHEARALASDGLLARTGWLDAARIDAL
HHHHHHHHCCHHHEECCCCCHHHHHHHHHHCHHHHHHHHHHCCCCEEECCCCCHHHHHHH
ADAHVSGRADHSRLIWQLLMLRKSLQLLG
HHCCCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]