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The map label for this gene is ykoW [H]

Identifier: 87200340

GI number: 87200340

Start: 2469082

End: 2471364

Strand: Reverse

Name: ykoW [H]

Synonym: Saro_2326

Alternate gene names: 87200340

Gene position: 2471364-2469082 (Counterclockwise)

Preceding gene: 87200341

Following gene: 87200334

Centisome position: 69.39

GC content: 59.13

Gene sequence:

>2283_bases
ATGCTGAAGAAACTGCGCTCACCGCTGTATCTCGCGCTGCTGATCCCGGCGTTGCTGGCGCTTACCGATCTTTTGGCGCC
CATTGATCACATATTCTGGAACACCCGATCCAAGTTCGGCAGCCATGCCGCCAGCGGAGCGACCGTAATTGTCAGTTTCG
ACGATGGCGTGCACGCGTCGGAAAAATCGGTTCCAAGCACTTCCCAAGTGGCCGATGTTCTGGATCGGCTGGCATCTCAG
ATGCCGCGTCGAATCTATCTCGACAGCCAGCTTAAGTTTGGGGTCGATCGCGCGGGTGACGAAGCTCTTGCGCGCGCGTT
GAAGAGGCTGCAGCCCGACGCCACTCTGGTCCTTAGGAGCCGAGAAATCGGCGGGCTCAAGCACAAGTTCGGTCCCGCCG
ATTTTGATATCCCGCCGAAGGGAACCATTGACGAGGTTCCAATCGTCCTGTCCGCGTGGGATGGGAACTTCATGGAGTTT
GGAGTTTCCTCTCCATACAAGATCACGATCGGCGGCAAGGTCTATCCAACTTTTGCGGCAGTGCTTGCGGAACGATTTGA
TGGGCTTCAGCCTTCGTTCGCCCCCGAGTATCTGCTGGATTTGGACTCCGTGCCGCAATTGAGCGCCACAAAATTCCTGC
GGGGGGATTTTCCAAACGGATTGCTCACTGGACGGACAGTCATTGTAAACTCGTCGGGCACGGCTCCTGCGATCGGCCTG
TATGGTCGTGGACGGGTGCACCCCTTGGCACTTGACCTTGCAGGGGCTGACGCCTTGCGCAAGCCGCTAGCCTTGGCCTT
GGGCTCTTATCCATTGCTGATGCTCTTCTGGCTGATGGTCCGCACGACTTCTCGCCTTTCGCGGAGGTCTTACAAGTTCG
TGGCCTACGCAGGGGCGTTGGCAGCGATCTTTGTATTGCCGGTGCTGCTGCAGATCGTGGGCATCACGATTGATATCGGC
GCTTCGCTGCTTTGTGCCATCGTCTATGGAAGCGGGCGACTGTGGCAACTGCGCGTGCGCCGTGTACAACACACCAGCGC
TTCCGGCCTGCCCAACCTGATCGCGCTGAGCGCCACTTCCTTCGAGGTCGGGCGCGACGTCGTTGTAGCGGTTATCGCCA
GATACGAAGAAATTCTCGCGACCCTTCCCAAGGAGTTGCACGGCGAATGTGCCAAGCAAATTGCCCGGCGCCTTTCCGTG
GGTTCTGGTATCGAAACAATCTTCAACGGCGAGGGGGGGCACTTTGCCTGGACGGAGGAAGCACGTCCGCTCGAGATGCA
ATTGAATCACCTCGAGGGCCTGCGTGCACTGTTCTCTGCTCCGCTGGAAATCGGAGCGTACACGTTCGACACCAACATCC
ACTTCGGGCTCGACCGCAATGAAGGTCTTGATGCCCTGACTCGCGTCAACTCCGCACTGGCAAGTGCAAACGATGCTCTC
AACAGTGGCAGGTCCGTCGAACTGTTCGAGGCCGATCGGCTTGCCGAGGCGACGTGGGAACTCTCCCTTCATGCGCGTAT
CGACGAAGGTTTGCGGAATGGCGAAATCTGGCTCGCCTACCAGAGCCAGTGGGACATGCGTGAAGGGCGCCCTTGCGGTG
CGGAAGCGCTGATCCGCTGGAATCATCCTACGCGCGGGCCGATCGCGCCGGACGCCTTCATCCTCCAGGCCGAACGCGCA
GGACGCATTGACGCTCTTACCTATTGGGTCATCGAGGAGGCGATTACTGCCGCGCTTGCGTTGAACGCGGGCGGGCAACC
CTTTCAGATGAGCGTCAACATGTCGGCGCAGATGGTCGACAAGCCCGATCTTGTGCCCAGCATCTGGGAGATCGTCCAGC
GTCGCAAGATTGACCCCGGGCTTCTCACCATCGAGATCACGGAAACGTCCAGCGTGCGCAACCGCCCTGCGGCAGTGCAG
AACCTCACTCGCCTTCGGGACATGGGCATGCGGTTGTCGATCGATGACTTCGGAACGGGCGAAGCAAGCCTTGCCTATTT
GGCCGATCTTCCGAGCGACGAACTCAAGCTGGACCGCCGCTTTGTCTCTCGTGTTTTGACGCACGAACGCGAACGCCTGA
TCGTCAAGTCGACGATCGAGTTGGCTCACGCGCTGGGGCAGGCAGTCGTCGCCGAGGGAATCGAGGATGACGCCACTTAC
CGAATGCTGCGTTCGCTGGGCTGCGATGTTGGGCAAGGGTACTTTCTCGGACGGCCACAACCTTTTGCCGAGCTAAGAAA
AACTGTTCTCGAATTGGGACGGCGCCAGATCGGACTCGTTTAG

Upstream 100 bases:

>100_bases
TTGCAGCTTCTTGGTTGATTGCCAATTAATCATGACATTTCGGTAAGACGTTCTTCACCATTGCGGTCTAGTCAGGCTGT
GAAACAACCGCCGATGGTCC

Downstream 100 bases:

>100_bases
AAGTTGTTAACCATACCTGTTGGTGTTAACCATGTGTCTCCAATCGTGGATGGAGGCACGAATGTTTGCAGCTCTCAACG
CGGTCATCGAGATCCTTCGC

Product: diguanylate phosphodiesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 760; Mature: 760

Protein sequence:

>760_residues
MLKKLRSPLYLALLIPALLALTDLLAPIDHIFWNTRSKFGSHAASGATVIVSFDDGVHASEKSVPSTSQVADVLDRLASQ
MPRRIYLDSQLKFGVDRAGDEALARALKRLQPDATLVLRSREIGGLKHKFGPADFDIPPKGTIDEVPIVLSAWDGNFMEF
GVSSPYKITIGGKVYPTFAAVLAERFDGLQPSFAPEYLLDLDSVPQLSATKFLRGDFPNGLLTGRTVIVNSSGTAPAIGL
YGRGRVHPLALDLAGADALRKPLALALGSYPLLMLFWLMVRTTSRLSRRSYKFVAYAGALAAIFVLPVLLQIVGITIDIG
ASLLCAIVYGSGRLWQLRVRRVQHTSASGLPNLIALSATSFEVGRDVVVAVIARYEEILATLPKELHGECAKQIARRLSV
GSGIETIFNGEGGHFAWTEEARPLEMQLNHLEGLRALFSAPLEIGAYTFDTNIHFGLDRNEGLDALTRVNSALASANDAL
NSGRSVELFEADRLAEATWELSLHARIDEGLRNGEIWLAYQSQWDMREGRPCGAEALIRWNHPTRGPIAPDAFILQAERA
GRIDALTYWVIEEAITAALALNAGGQPFQMSVNMSAQMVDKPDLVPSIWEIVQRRKIDPGLLTIEITETSSVRNRPAAVQ
NLTRLRDMGMRLSIDDFGTGEASLAYLADLPSDELKLDRRFVSRVLTHERERLIVKSTIELAHALGQAVVAEGIEDDATY
RMLRSLGCDVGQGYFLGRPQPFAELRKTVLELGRRQIGLV

Sequences:

>Translated_760_residues
MLKKLRSPLYLALLIPALLALTDLLAPIDHIFWNTRSKFGSHAASGATVIVSFDDGVHASEKSVPSTSQVADVLDRLASQ
MPRRIYLDSQLKFGVDRAGDEALARALKRLQPDATLVLRSREIGGLKHKFGPADFDIPPKGTIDEVPIVLSAWDGNFMEF
GVSSPYKITIGGKVYPTFAAVLAERFDGLQPSFAPEYLLDLDSVPQLSATKFLRGDFPNGLLTGRTVIVNSSGTAPAIGL
YGRGRVHPLALDLAGADALRKPLALALGSYPLLMLFWLMVRTTSRLSRRSYKFVAYAGALAAIFVLPVLLQIVGITIDIG
ASLLCAIVYGSGRLWQLRVRRVQHTSASGLPNLIALSATSFEVGRDVVVAVIARYEEILATLPKELHGECAKQIARRLSV
GSGIETIFNGEGGHFAWTEEARPLEMQLNHLEGLRALFSAPLEIGAYTFDTNIHFGLDRNEGLDALTRVNSALASANDAL
NSGRSVELFEADRLAEATWELSLHARIDEGLRNGEIWLAYQSQWDMREGRPCGAEALIRWNHPTRGPIAPDAFILQAERA
GRIDALTYWVIEEAITAALALNAGGQPFQMSVNMSAQMVDKPDLVPSIWEIVQRRKIDPGLLTIEITETSSVRNRPAAVQ
NLTRLRDMGMRLSIDDFGTGEASLAYLADLPSDELKLDRRFVSRVLTHERERLIVKSTIELAHALGQAVVAEGIEDDATY
RMLRSLGCDVGQGYFLGRPQPFAELRKTVLELGRRQIGLV
>Mature_760_residues
MLKKLRSPLYLALLIPALLALTDLLAPIDHIFWNTRSKFGSHAASGATVIVSFDDGVHASEKSVPSTSQVADVLDRLASQ
MPRRIYLDSQLKFGVDRAGDEALARALKRLQPDATLVLRSREIGGLKHKFGPADFDIPPKGTIDEVPIVLSAWDGNFMEF
GVSSPYKITIGGKVYPTFAAVLAERFDGLQPSFAPEYLLDLDSVPQLSATKFLRGDFPNGLLTGRTVIVNSSGTAPAIGL
YGRGRVHPLALDLAGADALRKPLALALGSYPLLMLFWLMVRTTSRLSRRSYKFVAYAGALAAIFVLPVLLQIVGITIDIG
ASLLCAIVYGSGRLWQLRVRRVQHTSASGLPNLIALSATSFEVGRDVVVAVIARYEEILATLPKELHGECAKQIARRLSV
GSGIETIFNGEGGHFAWTEEARPLEMQLNHLEGLRALFSAPLEIGAYTFDTNIHFGLDRNEGLDALTRVNSALASANDAL
NSGRSVELFEADRLAEATWELSLHARIDEGLRNGEIWLAYQSQWDMREGRPCGAEALIRWNHPTRGPIAPDAFILQAERA
GRIDALTYWVIEEAITAALALNAGGQPFQMSVNMSAQMVDKPDLVPSIWEIVQRRKIDPGLLTIEITETSSVRNRPAAVQ
NLTRLRDMGMRLSIDDFGTGEASLAYLADLPSDELKLDRRFVSRVLTHERERLIVKSTIELAHALGQAVVAEGIEDDATY
RMLRSLGCDVGQGYFLGRPQPFAELRKTVLELGRRQIGLV

Specific function: Probable signaling protein whose physiological role is not yet known [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI87081921, Length=318, Percent_Identity=32.7044025157233, Blast_Score=157, Evalue=3e-39,
Organism=Escherichia coli, GI226510982, Length=284, Percent_Identity=30.6338028169014, Blast_Score=136, Evalue=4e-33,
Organism=Escherichia coli, GI87081845, Length=246, Percent_Identity=33.3333333333333, Blast_Score=124, Evalue=3e-29,
Organism=Escherichia coli, GI1787055, Length=259, Percent_Identity=31.2741312741313, Blast_Score=122, Evalue=6e-29,
Organism=Escherichia coli, GI1787541, Length=304, Percent_Identity=27.9605263157895, Blast_Score=122, Evalue=8e-29,
Organism=Escherichia coli, GI1790496, Length=241, Percent_Identity=30.7053941908714, Blast_Score=117, Evalue=3e-27,
Organism=Escherichia coli, GI1788849, Length=246, Percent_Identity=29.2682926829268, Blast_Score=115, Evalue=1e-26,
Organism=Escherichia coli, GI1788502, Length=244, Percent_Identity=29.9180327868852, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI87081743, Length=241, Percent_Identity=29.4605809128631, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI87082096, Length=204, Percent_Identity=32.843137254902, Blast_Score=105, Evalue=8e-24,
Organism=Escherichia coli, GI1786507, Length=236, Percent_Identity=29.6610169491525, Blast_Score=103, Evalue=4e-23,
Organism=Escherichia coli, GI87081980, Length=255, Percent_Identity=29.0196078431373, Blast_Score=102, Evalue=9e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR001633
- InterPro:   IPR005330
- InterPro:   IPR000014 [H]

Pfam domain/function: PF00563 EAL; PF00990 GGDEF; PF03707 MHYT [H]

EC number: NA

Molecular weight: Translated: 83256; Mature: 83256

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: PS50883 EAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKKLRSPLYLALLIPALLALTDLLAPIDHIFWNTRSKFGSHAASGATVIVSFDDGVHAS
CCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCEEEEEECCCCCCC
EKSVPSTSQVADVLDRLASQMPRRIYLDSQLKFGVDRAGDEALARALKRLQPDATLVLRS
CCCCCCHHHHHHHHHHHHHHCCCEEEECCHHHCCCCCCCHHHHHHHHHHHCCCCEEEEEC
REIGGLKHKFGPADFDIPPKGTIDEVPIVLSAWDGNFMEFGVSSPYKITIGGKVYPTFAA
CCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEEECCCCCEEEEECCEEHHHHHH
VLAERFDGLQPSFAPEYLLDLDSVPQLSATKFLRGDFPNGLLTGRTVIVNSSGTAPAIGL
HHHHHHCCCCCCCCCHHHHCCCCCCCHHHHHHHHCCCCCCEEECCEEEEECCCCCCEEEC
YGRGRVHPLALDLAGADALRKPLALALGSYPLLMLFWLMVRTTSRLSRRSYKFVAYAGAL
CCCCCCCEEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
AAIFVLPVLLQIVGITIDIGASLLCAIVYGSGRLWQLRVRRVQHTSASGLPNLIALSATS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHCCCCCCCCEEEEECCC
FEVGRDVVVAVIARYEEILATLPKELHGECAKQIARRLSVGSGIETIFNGEGGHFAWTEE
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHEECCCCCCEEECCC
ARPLEMQLNHLEGLRALFSAPLEIGAYTFDTNIHFGLDRNEGLDALTRVNSALASANDAL
CCCHHHHHHHHHHHHHHHHCCHHHCEEEEECEEEECCCCCCCHHHHHHHHHHHHHHHHHH
NSGRSVELFEADRLAEATWELSLHARIDEGLRNGEIWLAYQSQWDMREGRPCGAEALIRW
CCCCCEEEEEHHHHHHHHHEEEEEEEHHCCCCCCEEEEEECCCCCCCCCCCCCCHHEEEE
NHPTRGPIAPDAFILQAERAGRIDALTYWVIEEAITAALALNAGGQPFQMSVNMSAQMVD
CCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEECC
KPDLVPSIWEIVQRRKIDPGLLTIEITETSSVRNRPAAVQNLTRLRDMGMRLSIDDFGTG
CCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCC
EASLAYLADLPSDELKLDRRFVSRVLTHERERLIVKSTIELAHALGQAVVAEGIEDDATY
CCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
RMLRSLGCDVGQGYFLGRPQPFAELRKTVLELGRRQIGLV
HHHHHHCCCCCCCEECCCCCCHHHHHHHHHHHCHHHCCCC
>Mature Secondary Structure
MLKKLRSPLYLALLIPALLALTDLLAPIDHIFWNTRSKFGSHAASGATVIVSFDDGVHAS
CCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCEEEEEECCCCCCC
EKSVPSTSQVADVLDRLASQMPRRIYLDSQLKFGVDRAGDEALARALKRLQPDATLVLRS
CCCCCCHHHHHHHHHHHHHHCCCEEEECCHHHCCCCCCCHHHHHHHHHHHCCCCEEEEEC
REIGGLKHKFGPADFDIPPKGTIDEVPIVLSAWDGNFMEFGVSSPYKITIGGKVYPTFAA
CCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEEECCCCCEEEEECCEEHHHHHH
VLAERFDGLQPSFAPEYLLDLDSVPQLSATKFLRGDFPNGLLTGRTVIVNSSGTAPAIGL
HHHHHHCCCCCCCCCHHHHCCCCCCCHHHHHHHHCCCCCCEEECCEEEEECCCCCCEEEC
YGRGRVHPLALDLAGADALRKPLALALGSYPLLMLFWLMVRTTSRLSRRSYKFVAYAGAL
CCCCCCCEEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
AAIFVLPVLLQIVGITIDIGASLLCAIVYGSGRLWQLRVRRVQHTSASGLPNLIALSATS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHCCCCCCCCEEEEECCC
FEVGRDVVVAVIARYEEILATLPKELHGECAKQIARRLSVGSGIETIFNGEGGHFAWTEE
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHEECCCCCCEEECCC
ARPLEMQLNHLEGLRALFSAPLEIGAYTFDTNIHFGLDRNEGLDALTRVNSALASANDAL
CCCHHHHHHHHHHHHHHHHCCHHHCEEEEECEEEECCCCCCCHHHHHHHHHHHHHHHHHH
NSGRSVELFEADRLAEATWELSLHARIDEGLRNGEIWLAYQSQWDMREGRPCGAEALIRW
CCCCCEEEEEHHHHHHHHHEEEEEEEHHCCCCCCEEEEEECCCCCCCCCCCCCCHHEEEE
NHPTRGPIAPDAFILQAERAGRIDALTYWVIEEAITAALALNAGGQPFQMSVNMSAQMVD
CCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEECC
KPDLVPSIWEIVQRRKIDPGLLTIEITETSSVRNRPAAVQNLTRLRDMGMRLSIDDFGTG
CCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCC
EASLAYLADLPSDELKLDRRFVSRVLTHERERLIVKSTIELAHALGQAVVAEGIEDDATY
CCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
RMLRSLGCDVGQGYFLGRPQPFAELRKTVLELGRRQIGLV
HHHHHHCCCCCCCEECCCCCCHHHHHHHHHHHCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 11728710 [H]