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The map label for this gene is glyA

Identifier: 87200254

GI number: 87200254

Start: 2382863

End: 2384173

Strand: Direct

Name: glyA

Synonym: Saro_2240

Alternate gene names: 87200254

Gene position: 2382863-2384173 (Clockwise)

Preceding gene: 87200253

Following gene: 87200255

Centisome position: 66.9

GC content: 64.38

Gene sequence:

>1311_bases
ATGACCACTGCCACCGCAGCGCCGGAAATCCGTAAAGCCGGCTTCTTCACCGAACATCTCGAGACCGCAGACGCCGAAGT
CTTCGCCGCGATCCGTGGCGAGCTGAAGCGCCAGCAGACCAAGATCGAACTGATCGCGTCCGAGAACATCACCAGCCTGG
CCGTGCTTGAAGCGACCGGTTCGGTCTTCACCAACAAGTACGCCGAAGGCTATCCGGGCAAGCGCTACTACGGCGGCTGC
GAATATGCAGACGTGGTCGAAAACCTCGCGATCGAGCGCGCGAAGAAGCTGTTCGGCTGCAACTTCGCCAACGTCCAGCC
CAACTCGGGCAGCCAGATGAACCAGGCCGTGTTCCTCGCGCTGCTTCAGCCTGGCGACAGCTTCATGGGCCTCGACCTCA
ATTCGGGCGGCCACCTGACGCACGGTTCGCCGGTCAACATGAGCGGCAAGTGGTTCAAGCCGATCCCCTATGGCGTGCGC
GCCGACGATCACCTGATCGACATGGACGAGGTTGCCCGCCTTGCCCGCGAGAACAAGCCCAAGCTGATCATCGCAGGCGG
CACTGCATATTCGCGCGTGTGGGATTTCAAGCGCTTCCGCGAGATCGCCGATGAAGTGGGCGCCTGGCTGCTGGTCGACA
TGTCGCACTTCTCCGGCCTCGTCGCTGGCGGTGCGCACCCCTCGCCCTTCCCGCACGCCCATGTCGTGACCAGCACGACG
CACAAGTCGCTGCGCGGTCCGCGTTCGGGCATCATCCTGACCAACGACGAGGATCTGGCGAAGAAGTTCAACATGGCGGT
GTTCCCCGGCATGCAGGGCGGCCCGCTAGTCCACGTGATCGCGGCAAAGGCGGTCGCGTTTGGCGAAGCGCTGCGTCCTG
AATTCAAGGCCTATGCCGCGCAGATCGTCGCCAACGCCCGCGCGCTGGCCGAAGCGGTCAAGGACGCCGGCCTTTCGGTC
GTCTCGGGTGGGACCGACAACCACCTGATGCTGGTCGATCTTTCTGCCAAGGACGTGACGGGCAAGGCCGCCGAGAAGGG
GCTCGACCGCGCCTGGCTGACCTGCAACAAGAACGGCGTCCCCTTCGACAAGCGCTCGCCCTTCGTCACGTCGGGCATCC
GCCTCGGCACCCCGGCGGGCACGACGCGCGGTTTCCGCGAGGAAGAGTTCCGCAAGATCGGCGCACTGATCGGTGAAGTG
GTCGATGGCCTTGCACGCAACGGCGAAGAGGGCGATGGTCAGGTGGAACAGCGCGTGCGCGACCGTGTCGCCGAGCTTTG
CGCGCAGTTCCCGATCTATCCGGAGCTTTGA

Upstream 100 bases:

>100_bases
CCTGCCTCGACGCTTTCCTTTCCACCGAATTCGGCGGAGGCCGCCATGCGGGCCGCGTCGAAAAGCTTTCCAATCCCGCG
ATCTGAAGGAGCCCCGAGAG

Downstream 100 bases:

>100_bases
GCGATGAACGACCGTGACGACCTCGCCACCCGTGCCAAGGAGGAGTTCTCCGCCATGGCCAAGCAGGGCATGGCCCATCC
GTCGACCAAGCCGGTGCTGA

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MTTATAAPEIRKAGFFTEHLETADAEVFAAIRGELKRQQTKIELIASENITSLAVLEATGSVFTNKYAEGYPGKRYYGGC
EYADVVENLAIERAKKLFGCNFANVQPNSGSQMNQAVFLALLQPGDSFMGLDLNSGGHLTHGSPVNMSGKWFKPIPYGVR
ADDHLIDMDEVARLARENKPKLIIAGGTAYSRVWDFKRFREIADEVGAWLLVDMSHFSGLVAGGAHPSPFPHAHVVTSTT
HKSLRGPRSGIILTNDEDLAKKFNMAVFPGMQGGPLVHVIAAKAVAFGEALRPEFKAYAAQIVANARALAEAVKDAGLSV
VSGGTDNHLMLVDLSAKDVTGKAAEKGLDRAWLTCNKNGVPFDKRSPFVTSGIRLGTPAGTTRGFREEEFRKIGALIGEV
VDGLARNGEEGDGQVEQRVRDRVAELCAQFPIYPEL

Sequences:

>Translated_436_residues
MTTATAAPEIRKAGFFTEHLETADAEVFAAIRGELKRQQTKIELIASENITSLAVLEATGSVFTNKYAEGYPGKRYYGGC
EYADVVENLAIERAKKLFGCNFANVQPNSGSQMNQAVFLALLQPGDSFMGLDLNSGGHLTHGSPVNMSGKWFKPIPYGVR
ADDHLIDMDEVARLARENKPKLIIAGGTAYSRVWDFKRFREIADEVGAWLLVDMSHFSGLVAGGAHPSPFPHAHVVTSTT
HKSLRGPRSGIILTNDEDLAKKFNMAVFPGMQGGPLVHVIAAKAVAFGEALRPEFKAYAAQIVANARALAEAVKDAGLSV
VSGGTDNHLMLVDLSAKDVTGKAAEKGLDRAWLTCNKNGVPFDKRSPFVTSGIRLGTPAGTTRGFREEEFRKIGALIGEV
VDGLARNGEEGDGQVEQRVRDRVAELCAQFPIYPEL
>Mature_435_residues
TTATAAPEIRKAGFFTEHLETADAEVFAAIRGELKRQQTKIELIASENITSLAVLEATGSVFTNKYAEGYPGKRYYGGCE
YADVVENLAIERAKKLFGCNFANVQPNSGSQMNQAVFLALLQPGDSFMGLDLNSGGHLTHGSPVNMSGKWFKPIPYGVRA
DDHLIDMDEVARLARENKPKLIIAGGTAYSRVWDFKRFREIADEVGAWLLVDMSHFSGLVAGGAHPSPFPHAHVVTSTTH
KSLRGPRSGIILTNDEDLAKKFNMAVFPGMQGGPLVHVIAAKAVAFGEALRPEFKAYAAQIVANARALAEAVKDAGLSVV
SGGTDNHLMLVDLSAKDVTGKAAEKGLDRAWLTCNKNGVPFDKRSPFVTSGIRLGTPAGTTRGFREEEFRKIGALIGEVV
DGLARNGEEGDGQVEQRVRDRVAELCAQFPIYPEL

Specific function: Interconversion of serine and glycine

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family

Homologues:

Organism=Homo sapiens, GI261862352, Length=453, Percent_Identity=46.1368653421634, Blast_Score=362, Evalue=1e-100,
Organism=Homo sapiens, GI261862350, Length=453, Percent_Identity=46.1368653421634, Blast_Score=362, Evalue=1e-100,
Organism=Homo sapiens, GI261862348, Length=453, Percent_Identity=46.1368653421634, Blast_Score=362, Evalue=1e-100,
Organism=Homo sapiens, GI19923315, Length=453, Percent_Identity=46.1368653421634, Blast_Score=362, Evalue=1e-100,
Organism=Homo sapiens, GI261862346, Length=453, Percent_Identity=45.4746136865342, Blast_Score=349, Evalue=2e-96,
Organism=Homo sapiens, GI22547186, Length=406, Percent_Identity=48.7684729064039, Blast_Score=346, Evalue=3e-95,
Organism=Homo sapiens, GI22547189, Length=392, Percent_Identity=46.4285714285714, Blast_Score=314, Evalue=1e-85,
Organism=Escherichia coli, GI1788902, Length=412, Percent_Identity=57.5242718446602, Blast_Score=464, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI25144732, Length=409, Percent_Identity=48.8997555012225, Blast_Score=358, Evalue=2e-99,
Organism=Caenorhabditis elegans, GI25144729, Length=409, Percent_Identity=48.8997555012225, Blast_Score=358, Evalue=3e-99,
Organism=Saccharomyces cerevisiae, GI6319739, Length=432, Percent_Identity=44.6759259259259, Blast_Score=348, Evalue=1e-96,
Organism=Saccharomyces cerevisiae, GI6323087, Length=411, Percent_Identity=44.7688564476886, Blast_Score=338, Evalue=1e-93,
Organism=Drosophila melanogaster, GI24640005, Length=454, Percent_Identity=44.273127753304, Blast_Score=360, Evalue=1e-99,
Organism=Drosophila melanogaster, GI221329721, Length=454, Percent_Identity=44.273127753304, Blast_Score=359, Evalue=2e-99,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): GLYA_NOVAD (Q2G646)

Other databases:

- EMBL:   CP000248
- RefSeq:   YP_497511.1
- ProteinModelPortal:   Q2G646
- SMR:   Q2G646
- STRING:   Q2G646
- GeneID:   3916556
- GenomeReviews:   CP000248_GR
- KEGG:   nar:Saro_2240
- eggNOG:   COG0112
- HOGENOM:   HBG301263
- OMA:   CEGLEMI
- PhylomeDB:   Q2G646
- ProtClustDB:   PRK00011
- BioCyc:   NARO279238:SARO_2240-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00051_B
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11680
- PIRSF:   PIRSF000412

Pfam domain/function: PF00464 SHMT; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.1.2.1

Molecular weight: Translated: 47075; Mature: 46944

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTATAAPEIRKAGFFTEHLETADAEVFAAIRGELKRQQTKIELIASENITSLAVLEATG
CCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCEEEEEECCC
SVFTNKYAEGYPGKRYYGGCEYADVVENLAIERAKKLFGCNFANVQPNSGSQMNQAVFLA
HHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHEEEEE
LLQPGDSFMGLDLNSGGHLTHGSPVNMSGKWFKPIPYGVRADDHLIDMDEVARLARENKP
EECCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECHHHHHHHHHCCCC
KLIIAGGTAYSRVWDFKRFREIADEVGAWLLVDMSHFSGLVAGGAHPSPFPHAHVVTSTT
CEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEEHHHHCCEEECCCCCCCCCCCEEEECCH
HKSLRGPRSGIILTNDEDLAKKFNMAVFPGMQGGPLVHVIAAKAVAFGEALRPEFKAYAA
HHHHCCCCCCEEEECCHHHHHHCCCEECCCCCCCCEEHHHHHHHHHHHHHHCHHHHHHHH
QIVANARALAEAVKDAGLSVVSGGTDNHLMLVDLSAKDVTGKAAEKGLDRAWLTCNKNGV
HHHHHHHHHHHHHHHCCCEEEECCCCCEEEEEECCCCCCCCHHHHHCCCCEEEEECCCCC
PFDKRSPFVTSGIRLGTPAGTTRGFREEEFRKIGALIGEVVDGLARNGEEGDGQVEQRVR
CCCCCCCCEECCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
DRVAELCAQFPIYPEL
HHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TTATAAPEIRKAGFFTEHLETADAEVFAAIRGELKRQQTKIELIASENITSLAVLEATG
CCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCEEEEEECCC
SVFTNKYAEGYPGKRYYGGCEYADVVENLAIERAKKLFGCNFANVQPNSGSQMNQAVFLA
HHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHEEEEE
LLQPGDSFMGLDLNSGGHLTHGSPVNMSGKWFKPIPYGVRADDHLIDMDEVARLARENKP
EECCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECHHHHHHHHHCCCC
KLIIAGGTAYSRVWDFKRFREIADEVGAWLLVDMSHFSGLVAGGAHPSPFPHAHVVTSTT
CEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEEHHHHCCEEECCCCCCCCCCCEEEECCH
HKSLRGPRSGIILTNDEDLAKKFNMAVFPGMQGGPLVHVIAAKAVAFGEALRPEFKAYAA
HHHHCCCCCCEEEECCHHHHHHCCCEECCCCCCCCEEHHHHHHHHHHHHHHCHHHHHHHH
QIVANARALAEAVKDAGLSVVSGGTDNHLMLVDLSAKDVTGKAAEKGLDRAWLTCNKNGV
HHHHHHHHHHHHHHHCCCEEEECCCCCEEEEEECCCCCCCCHHHHHCCCCEEEEECCCCC
PFDKRSPFVTSGIRLGTPAGTTRGFREEEFRKIGALIGEVVDGLARNGEEGDGQVEQRVR
CCCCCCCCEECCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
DRVAELCAQFPIYPEL
HHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA