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The map label for this gene is ribH

Identifier: 87200231

GI number: 87200231

Start: 2360752

End: 2361171

Strand: Direct

Name: ribH

Synonym: Saro_2216

Alternate gene names: 87200231

Gene position: 2360752-2361171 (Clockwise)

Preceding gene: 87200229

Following gene: 87200236

Centisome position: 66.28

GC content: 64.76

Gene sequence:

>420_bases
ATGGCCAAGTTCCTCATCGTCGAAGCCCGGTTCTACGATCACCTGAACGACATGCTCGTCGCCGGAGCCAAGGCCGCGCT
CAAGGAAGCCGGGCACGAGGTCGAAGTCATCACCGTGCCGGGCGCCCTTGAAATTCCGGGCGCGATCGCGCTCGCCGACC
AGTCGGAAGACTATGACGGCTATGTTGCCATCGGCGTCGTCATCCGCGGCGAAACGTATCACTTCGAGATCGTCGCCGGC
GAAAGCGCACGCGGCATCATGGCACTGACGATGGACGGCGTTGCAATCGGCAACGGCATTCTCACGGTCGAAAACGAAGC
CCAGGCCCTCGTCCGCGCCGACCCGAAGCAGAAGGACAAGGGCGGCGAGGCTGCCAAGGCCGCCCTCGCCCTCCTCGCGC
TGCGCGAACGCTGGAGCTGA

Upstream 100 bases:

>100_bases
GTGACTCGGGTCATCAGGCTGGCAAAGACGCAACACGTTGCCTAGAGGCATCGCCATCCCTTCATCGGCGCTGGGCCGAA
ATTTCCAAGGACAGTTTCCC

Downstream 100 bases:

>100_bases
GGCTCAACCCAGAGGCGCGCAGCGGTCACCGCGGCGCGCCTTGTCGGCAGCCTCGAACGCGGCCTGGGTCGGGGCAAGCC
TTCGATCGAGAATGATGCCC

Product: 6,7-dimethyl-8-ribityllumazine synthase

Products: NA

Alternate protein names: DMRL synthase; Lumazine synthase; Riboflavin synthase beta chain

Number of amino acids: Translated: 139; Mature: 138

Protein sequence:

>139_residues
MAKFLIVEARFYDHLNDMLVAGAKAALKEAGHEVEVITVPGALEIPGAIALADQSEDYDGYVAIGVVIRGETYHFEIVAG
ESARGIMALTMDGVAIGNGILTVENEAQALVRADPKQKDKGGEAAKAALALLALRERWS

Sequences:

>Translated_139_residues
MAKFLIVEARFYDHLNDMLVAGAKAALKEAGHEVEVITVPGALEIPGAIALADQSEDYDGYVAIGVVIRGETYHFEIVAG
ESARGIMALTMDGVAIGNGILTVENEAQALVRADPKQKDKGGEAAKAALALLALRERWS
>Mature_138_residues
AKFLIVEARFYDHLNDMLVAGAKAALKEAGHEVEVITVPGALEIPGAIALADQSEDYDGYVAIGVVIRGETYHFEIVAGE
SARGIMALTMDGVAIGNGILTVENEAQALVRADPKQKDKGGEAAKAALALLALRERWS

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes

COG id: COG0054

COG function: function code H; Riboflavin synthase beta-chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DMRL synthase family

Homologues:

Organism=Escherichia coli, GI1786617, Length=136, Percent_Identity=40.4411764705882, Blast_Score=88, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RISB_NOVAD (Q2G669)

Other databases:

- EMBL:   CP000248
- RefSeq:   YP_497488.1
- ProteinModelPortal:   Q2G669
- SMR:   Q2G669
- STRING:   Q2G669
- GeneID:   3916532
- GenomeReviews:   CP000248_GR
- KEGG:   nar:Saro_2216
- NMPDR:   fig|48935.1.peg.1205
- eggNOG:   COG0054
- HOGENOM:   HBG311126
- OMA:   LGLQGAC
- ProtClustDB:   PRK00061
- BioCyc:   NARO279238:SARO_2216-MONOMER
- HAMAP:   MF_00178
- InterPro:   IPR002180
- Gene3D:   G3DSA:3.40.50.960
- PANTHER:   PTHR21058
- TIGRFAMs:   TIGR00114

Pfam domain/function: PF00885 DMRL_synthase; SSF52121 DMRL_synthase

EC number: =2.5.1.9

Molecular weight: Translated: 14720; Mature: 14589

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKFLIVEARFYDHLNDMLVAGAKAALKEAGHEVEVITVPGALEIPGAIALADQSEDYDG
CCEEEEEEHHHHHHHHHHHHHCHHHHHHHCCCEEEEEECCCCEECCCEEEEECCCCCCCC
YVAIGVVIRGETYHFEIVAGESARGIMALTMDGVAIGNGILTVENEAQALVRADPKQKDK
EEEEEEEEECCEEEEEEEECCCCCCEEEEEECCEEECCCEEEEECCCCEEEECCCCCCCC
GGEAAKAALALLALRERWS
CCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AKFLIVEARFYDHLNDMLVAGAKAALKEAGHEVEVITVPGALEIPGAIALADQSEDYDG
CEEEEEEHHHHHHHHHHHHHCHHHHHHHCCCEEEEEECCCCEECCCEEEEECCCCCCCC
YVAIGVVIRGETYHFEIVAGESARGIMALTMDGVAIGNGILTVENEAQALVRADPKQKDK
EEEEEEEEECCEEEEEEEECCCCCCEEEEEECCEEECCCEEEEECCCCEEEECCCCCCCC
GGEAAKAALALLALRERWS
CCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA