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The map label for this gene is tyrS

Identifier: 87200116

GI number: 87200116

Start: 2236438

End: 2237667

Strand: Direct

Name: tyrS

Synonym: Saro_2100

Alternate gene names: 87200116

Gene position: 2236438-2237667 (Clockwise)

Preceding gene: 87200113

Following gene: 87200117

Centisome position: 62.79

GC content: 65.12

Gene sequence:

>1230_bases
ATGACCGAATACGCTTCTTCCCTGCTGCGCCTGCTCTCGGAGCGGGGCTACATCCATCAGATGACCGACGCCGACGCACT
CGATGCGCTGGCGGCGAAGCAGGTCATACCCGGCTATATCGGCTTCGATCCGACCGCGCCATCGCTGCACGTCGGGTCGA
TGGTGCAGATCATGCTCCTGCGCCGCCTCCAGCAGGCCGGGCACAAGCCCATCGTGCTGATGGGCGGCGGCACCGGCAAG
ATCGGCGACCCGAGCTTCAAGGACGAGGCACGCAAGCTGATGACCAACGACGTCATCGCGGCCAACGTCGCCTCGATCAA
GACCGTGTTCGAACGCTTCCTGACCTTCGGCGACGGCCCGACCGACGCGGTCATGGTCGACAATGCCGACTGGCTCGACC
GGCTTGAATACATCCCGTTCCTGCGCGAGGTGGGCCAGCACTTCTCGGTCAACCGCATGCTCAGCTTCGATTCGGTGAAG
CAGCGCCTTGACCGCGAGCAATCGCTCTCGTTCCTCGAATTCAACTACATGATCCTCCAGGCCTACGACTTCCGCGAGCT
GTCGCAGCGCCACGCTTGCCGCCTGCAGATGGGCGGGTCGGATCAGTGGGGGAACATCGTCAACGGCATCGAACTGACCC
GCCGCATGGACGGCGTGGAAGTGTTCGGCGTGACCACGCCGCTGCTCACCACCGCCGACGGCTCCAAGATGGGGAAGACC
GCCGCTGGTGCTGTCTGGCTCAACGAGGATGCGCTCCCGGCCTGGGACTTCTGGCAATACTGGCGCAACACCGATGACCG
CGACGTGGGCAAGTTCCTGCGCCTGTTCACCGACCTGCCGCTGGACGAGATCGCCCGCCTCGAAGCGCTCGAGGGCAGCG
AGATCAACGCCGCCAAGGTCGTTCTGGCCAACGAGGTCACCAGACTGGTGCGCGGCGAGGAAGCAGCAAAGGCTGCCGAA
GCGACCGCGGCGGCGACCTTTGCGGGCGGCGGCCTCGGGCAGGATCTGCCGACCCTTTCCGTCGGCGAATCCGAGATCGG
CATCGTCGATGCGCTCGTCGGTCTGGGCTTTGCCGCCAGCCGTGGCGAGGCCAAGCGGCTCGTCGCGGGCGGCGGCGCGC
GCGTGGATGGCGAGCCAGTGACCGACGAGGGTTTCCGCATTCTTGTGAATGACAAGGAAATTCGCGTTTCTTCCGGCAAG
AAGAAGCACGGCATCCTGCGCAAGGCCTGA

Upstream 100 bases:

>100_bases
GTCACACCCAGCGGCGGGTTGGCCGGATGAGGCAACAGCTCATGCGTTTCCAATCGCGAAACTCCGTGCTAGTCGCGCCA
TCCCGACAAAGGTGGCCGAG

Downstream 100 bases:

>100_bases
CAGAGGCAGATTTCGCAAGACCGGCAAAAAGGTGCCGCCAGCCGGCACCGTTTGTCCGTAAATTTACGAATTATTCGCCA
ATCTGGAGGTATATCCCCTG

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS

Number of amino acids: Translated: 409; Mature: 408

Protein sequence:

>409_residues
MTEYASSLLRLLSERGYIHQMTDADALDALAAKQVIPGYIGFDPTAPSLHVGSMVQIMLLRRLQQAGHKPIVLMGGGTGK
IGDPSFKDEARKLMTNDVIAANVASIKTVFERFLTFGDGPTDAVMVDNADWLDRLEYIPFLREVGQHFSVNRMLSFDSVK
QRLDREQSLSFLEFNYMILQAYDFRELSQRHACRLQMGGSDQWGNIVNGIELTRRMDGVEVFGVTTPLLTTADGSKMGKT
AAGAVWLNEDALPAWDFWQYWRNTDDRDVGKFLRLFTDLPLDEIARLEALEGSEINAAKVVLANEVTRLVRGEEAAKAAE
ATAAATFAGGGLGQDLPTLSVGESEIGIVDALVGLGFAASRGEAKRLVAGGGARVDGEPVTDEGFRILVNDKEIRVSSGK
KKHGILRKA

Sequences:

>Translated_409_residues
MTEYASSLLRLLSERGYIHQMTDADALDALAAKQVIPGYIGFDPTAPSLHVGSMVQIMLLRRLQQAGHKPIVLMGGGTGK
IGDPSFKDEARKLMTNDVIAANVASIKTVFERFLTFGDGPTDAVMVDNADWLDRLEYIPFLREVGQHFSVNRMLSFDSVK
QRLDREQSLSFLEFNYMILQAYDFRELSQRHACRLQMGGSDQWGNIVNGIELTRRMDGVEVFGVTTPLLTTADGSKMGKT
AAGAVWLNEDALPAWDFWQYWRNTDDRDVGKFLRLFTDLPLDEIARLEALEGSEINAAKVVLANEVTRLVRGEEAAKAAE
ATAAATFAGGGLGQDLPTLSVGESEIGIVDALVGLGFAASRGEAKRLVAGGGARVDGEPVTDEGFRILVNDKEIRVSSGK
KKHGILRKA
>Mature_408_residues
TEYASSLLRLLSERGYIHQMTDADALDALAAKQVIPGYIGFDPTAPSLHVGSMVQIMLLRRLQQAGHKPIVLMGGGTGKI
GDPSFKDEARKLMTNDVIAANVASIKTVFERFLTFGDGPTDAVMVDNADWLDRLEYIPFLREVGQHFSVNRMLSFDSVKQ
RLDREQSLSFLEFNYMILQAYDFRELSQRHACRLQMGGSDQWGNIVNGIELTRRMDGVEVFGVTTPLLTTADGSKMGKTA
AGAVWLNEDALPAWDFWQYWRNTDDRDVGKFLRLFTDLPLDEIARLEALEGSEINAAKVVLANEVTRLVRGEEAAKAAEA
TAAATFAGGGLGQDLPTLSVGESEIGIVDALVGLGFAASRGEAKRLVAGGGARVDGEPVTDEGFRILVNDKEIRVSSGKK
KHGILRKA

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr)

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain

Homologues:

Organism=Homo sapiens, GI94681057, Length=298, Percent_Identity=39.9328859060403, Blast_Score=216, Evalue=2e-56,
Organism=Escherichia coli, GI1787925, Length=401, Percent_Identity=43.3915211970075, Blast_Score=331, Evalue=6e-92,
Organism=Caenorhabditis elegans, GI25145802, Length=312, Percent_Identity=34.9358974358974, Blast_Score=176, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI25145805, Length=244, Percent_Identity=32.3770491803279, Blast_Score=119, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6325160, Length=438, Percent_Identity=32.1917808219178, Blast_Score=224, Evalue=3e-59,
Organism=Drosophila melanogaster, GI20130383, Length=433, Percent_Identity=36.0277136258661, Blast_Score=238, Evalue=4e-63,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYY_NOVAD (Q2G6I4)

Other databases:

- EMBL:   CP000248
- RefSeq:   YP_497373.1
- ProteinModelPortal:   Q2G6I4
- SMR:   Q2G6I4
- STRING:   Q2G6I4
- GeneID:   3917748
- GenomeReviews:   CP000248_GR
- KEGG:   nar:Saro_2100
- NMPDR:   fig|48935.1.peg.1099
- eggNOG:   COG0162
- HOGENOM:   HBG288125
- OMA:   WINQDDR
- PhylomeDB:   Q2G6I4
- ProtClustDB:   PRK05912
- BioCyc:   NARO279238:SARO_2100-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_02006
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11766
- PRINTS:   PR01040
- SMART:   SM00363
- TIGRFAMs:   TIGR00234

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b

EC number: =6.1.1.1

Molecular weight: Translated: 44696; Mature: 44565

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50889 S4

Important sites: BINDING 39-39 BINDING 176-176 BINDING 180-180 BINDING 239-239

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEYASSLLRLLSERGYIHQMTDADALDALAAKQVIPGYIGFDPTAPSLHVGSMVQIMLL
CCHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHH
RRLQQAGHKPIVLMGGGTGKIGDPSFKDEARKLMTNDVIAANVASIKTVFERFLTFGDGP
HHHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
TDAVMVDNADWLDRLEYIPFLREVGQHFSVNRMLSFDSVKQRLDREQSLSFLEFNYMILQ
CCEEEECCHHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHCHHHCCHHHEEEEEEEE
AYDFRELSQRHACRLQMGGSDQWGNIVNGIELTRRMDGVEVFGVTTPLLTTADGSKMGKT
ECCHHHHHHHHCEEEECCCCCCCCHHHHHHHHHHHCCCEEEEEECCCEEECCCCCCCCCH
AAGAVWLNEDALPAWDFWQYWRNTDDRDVGKFLRLFTDLPLDEIARLEALEGSEINAAKV
HCCEEEECCCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHH
VLANEVTRLVRGEEAAKAAEATAAATFAGGGLGQDLPTLSVGESEIGIVDALVGLGFAAS
HHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCC
RGEAKRLVAGGGARVDGEPVTDEGFRILVNDKEIRVSSGKKKHGILRKA
CCCHHEEEECCCCCCCCCCCCCCCCEEEEECCEEEECCCCHHCCCCCCC
>Mature Secondary Structure 
TEYASSLLRLLSERGYIHQMTDADALDALAAKQVIPGYIGFDPTAPSLHVGSMVQIMLL
CHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHH
RRLQQAGHKPIVLMGGGTGKIGDPSFKDEARKLMTNDVIAANVASIKTVFERFLTFGDGP
HHHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
TDAVMVDNADWLDRLEYIPFLREVGQHFSVNRMLSFDSVKQRLDREQSLSFLEFNYMILQ
CCEEEECCHHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHCHHHCCHHHEEEEEEEE
AYDFRELSQRHACRLQMGGSDQWGNIVNGIELTRRMDGVEVFGVTTPLLTTADGSKMGKT
ECCHHHHHHHHCEEEECCCCCCCCHHHHHHHHHHHCCCEEEEEECCCEEECCCCCCCCCH
AAGAVWLNEDALPAWDFWQYWRNTDDRDVGKFLRLFTDLPLDEIARLEALEGSEINAAKV
HCCEEEECCCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHH
VLANEVTRLVRGEEAAKAAEATAAATFAGGGLGQDLPTLSVGESEIGIVDALVGLGFAAS
HHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCC
RGEAKRLVAGGGARVDGEPVTDEGFRILVNDKEIRVSSGKKKHGILRKA
CCCHHEEEECCCCCCCCCCCCCCCCEEEEECCEEEECCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA