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The map label for this gene is citA [H]

Identifier: 87200113

GI number: 87200113

Start: 2233152

End: 2234426

Strand: Direct

Name: citA [H]

Synonym: Saro_2097

Alternate gene names: 87200113

Gene position: 2233152-2234426 (Clockwise)

Preceding gene: 87200112

Following gene: 87200116

Centisome position: 62.7

GC content: 68.47

Gene sequence:

>1275_bases
GTGGCCGCGCAGACACTCGATGAGCGAAGACTTGCCCGCCGCGCCGTGATTGCCGCGACCACAGGCAACGCGCTGGAATT
CTACGATTTCATCACGTTCAGTTTCTTTGCCATCCAGATCGGCAGGGTCTTCTTCCCGTCGGAGGACCCTTTCGTCAGCC
TGATGGCCTCGCTCGCCACGTTCGGAGTCGGCTTCATCGGCCGCCCGCTCGGCGCATGGGCCATCGGCGCGTGGGCGGAC
CGGCACGGGCGCAAGCCGGCGATGCTGCTCAGCATGACGCTGATGGGCATCTCCGTCGCGGTCCTCGCGCTCACGCCGTC
CCATGCCGCCATCGGCGCCGCCGCCCCGGTCATCGTCGTGCTGGCCCGGCTGGTCCAGGGCTTCGCGCTCGGCGGAGAGG
TCGGCTCGGCAACCACCTACATGATGGAATGCGCCAGCCATGATCGCCGGGCCTGGGCGATAAGCTGGCAAGGCGCCAGC
CAGGCCATCGCATCTTCCGCCGGATCGCTGGTCGGCCTCGGCCTCAGCCTTGTCCTGACGCCGGACCAGCTGACCGACTG
GGGCTGGCGCGTGGCCCTGCTCGCGGGGACGGTGATCGTGCCGTTCTCTCTCGTCATCCGCCGGTCGCTTCCCGAAACGA
TCGATGCGCCCGATCACGTGCCCGCCGGGCACATCCCGCCCGGCGTGTGGCGGACGGTCGTGCTGGGCATGATGATGGTC
TCCGGGGCGACCATCGCAACCTACCTGTTCAATTACATGGCGACCTATGGCCAGAACACGCTCGGCTTCACGGCCAGCGT
CTCGCTGGGCACGACGCTGGCCGTCAACGTCGCGCGCTTCGCCGCGATCCTGCTGGGCGGCTGGCTCAGCGACCGCTTCG
GGCGGCGTCCCCTGATGATCCTGCCCTGGGCGGTCTTCGCCGCGGCCATCGTGCCGGCCTATGTCTGGCTGACATCGGCG
CACGACGCCTTCGTCTTCATCGCGGTCAACACCGCGCTCGCCTTCTGCTCGACGGTGCCTTCCGGCGCTGTCTACGCCGC
GATTGCCGAAAGCCTGCCCAAGGCCAGCCGCGCGAAGACCTTCGCGCTGGTCTACGCGCTGCCGGTCACTTTCCTGGGCG
GATCGACGCAGTTCGTCATCACCTGGCTGCTCAAGGTCACCGGAGAACCCATGGCGGTCGCCTGGTACATGCTCGGCGCC
GCGCTCCTCGCGCTTGCCGGGATGGTTCTCGTTCGCGAGAGCGCGCCCTCCCGCCTGCGCGCCTCTCCGGCCTGA

Upstream 100 bases:

>100_bases
ACCGGCTCCCCGCGCCGCTGCCCCCTCACACCCCGGCATTCCTTGACTCCCGCCCGGCATCTGCCAGCCTCGCCAGGAAC
TCGATCCACAGGGGGTGATC

Downstream 100 bases:

>100_bases
CCGGACCGGCCTGACGAAAGCGCTGTCCTACAGGGCCGGCGAGGGATTACACCCTTCCCGCTCCTTGAACCTTGAAGCCA
CCGATCCCCGCGCGAAAGGC

Product: general substrate transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter; Citrate utilization determinant; Citrate utilization protein A [H]

Number of amino acids: Translated: 424; Mature: 423

Protein sequence:

>424_residues
MAAQTLDERRLARRAVIAATTGNALEFYDFITFSFFAIQIGRVFFPSEDPFVSLMASLATFGVGFIGRPLGAWAIGAWAD
RHGRKPAMLLSMTLMGISVAVLALTPSHAAIGAAAPVIVVLARLVQGFALGGEVGSATTYMMECASHDRRAWAISWQGAS
QAIASSAGSLVGLGLSLVLTPDQLTDWGWRVALLAGTVIVPFSLVIRRSLPETIDAPDHVPAGHIPPGVWRTVVLGMMMV
SGATIATYLFNYMATYGQNTLGFTASVSLGTTLAVNVARFAAILLGGWLSDRFGRRPLMILPWAVFAAAIVPAYVWLTSA
HDAFVFIAVNTALAFCSTVPSGAVYAAIAESLPKASRAKTFALVYALPVTFLGGSTQFVITWLLKVTGEPMAVAWYMLGA
ALLALAGMVLVRESAPSRLRASPA

Sequences:

>Translated_424_residues
MAAQTLDERRLARRAVIAATTGNALEFYDFITFSFFAIQIGRVFFPSEDPFVSLMASLATFGVGFIGRPLGAWAIGAWAD
RHGRKPAMLLSMTLMGISVAVLALTPSHAAIGAAAPVIVVLARLVQGFALGGEVGSATTYMMECASHDRRAWAISWQGAS
QAIASSAGSLVGLGLSLVLTPDQLTDWGWRVALLAGTVIVPFSLVIRRSLPETIDAPDHVPAGHIPPGVWRTVVLGMMMV
SGATIATYLFNYMATYGQNTLGFTASVSLGTTLAVNVARFAAILLGGWLSDRFGRRPLMILPWAVFAAAIVPAYVWLTSA
HDAFVFIAVNTALAFCSTVPSGAVYAAIAESLPKASRAKTFALVYALPVTFLGGSTQFVITWLLKVTGEPMAVAWYMLGA
ALLALAGMVLVRESAPSRLRASPA
>Mature_423_residues
AAQTLDERRLARRAVIAATTGNALEFYDFITFSFFAIQIGRVFFPSEDPFVSLMASLATFGVGFIGRPLGAWAIGAWADR
HGRKPAMLLSMTLMGISVAVLALTPSHAAIGAAAPVIVVLARLVQGFALGGEVGSATTYMMECASHDRRAWAISWQGASQ
AIASSAGSLVGLGLSLVLTPDQLTDWGWRVALLAGTVIVPFSLVIRRSLPETIDAPDHVPAGHIPPGVWRTVVLGMMMVS
GATIATYLFNYMATYGQNTLGFTASVSLGTTLAVNVARFAAILLGGWLSDRFGRRPLMILPWAVFAAAIVPAYVWLTSAH
DAFVFIAVNTALAFCSTVPSGAVYAAIAESLPKASRAKTFALVYALPVTFLGGSTQFVITWLLKVTGEPMAVAWYMLGAA
LLALAGMVLVRESAPSRLRASPA

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=442, Percent_Identity=28.2805429864253, Blast_Score=182, Evalue=3e-47,
Organism=Escherichia coli, GI1788942, Length=420, Percent_Identity=33.3333333333333, Blast_Score=167, Evalue=2e-42,
Organism=Escherichia coli, GI1788292, Length=430, Percent_Identity=28.8372093023256, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1789941, Length=323, Percent_Identity=33.4365325077399, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI87082231, Length=223, Percent_Identity=28.2511210762332, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI87082404, Length=379, Percent_Identity=23.2189973614776, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 44801; Mature: 44670

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAQTLDERRLARRAVIAATTGNALEFYDFITFSFFAIQIGRVFFPSEDPFVSLMASLAT
CCCCHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHH
FGVGFIGRPLGAWAIGAWADRHGRKPAMLLSMTLMGISVAVLALTPSHAAIGAAAPVIVV
HHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHH
LARLVQGFALGGEVGSATTYMMECASHDRRAWAISWQGASQAIASSAGSLVGLGLSLVLT
HHHHHHHHCCCCCCCCHHHHHHHHHCCCCEEEEEEECCHHHHHHHCCCCCEECCEEEEEC
PDQLTDWGWRVALLAGTVIVPFSLVIRRSLPETIDAPDHVPAGHIPPGVWRTVVLGMMMV
CCHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
SGATIATYLFNYMATYGQNTLGFTASVSLGTTLAVNVARFAAILLGGWLSDRFGRRPLMI
CCHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
LPWAVFAAAIVPAYVWLTSAHDAFVFIAVNTALAFCSTVPSGAVYAAIAESLPKASRAKT
ECHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHH
FALVYALPVTFLGGSTQFVITWLLKVTGEPMAVAWYMLGAALLALAGMVLVRESAPSRLR
HHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
ASPA
CCCC
>Mature Secondary Structure 
AAQTLDERRLARRAVIAATTGNALEFYDFITFSFFAIQIGRVFFPSEDPFVSLMASLAT
CCCHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHH
FGVGFIGRPLGAWAIGAWADRHGRKPAMLLSMTLMGISVAVLALTPSHAAIGAAAPVIVV
HHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHH
LARLVQGFALGGEVGSATTYMMECASHDRRAWAISWQGASQAIASSAGSLVGLGLSLVLT
HHHHHHHHCCCCCCCCHHHHHHHHHCCCCEEEEEEECCHHHHHHHCCCCCEECCEEEEEC
PDQLTDWGWRVALLAGTVIVPFSLVIRRSLPETIDAPDHVPAGHIPPGVWRTVVLGMMMV
CCHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
SGATIATYLFNYMATYGQNTLGFTASVSLGTTLAVNVARFAAILLGGWLSDRFGRRPLMI
CCHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
LPWAVFAAAIVPAYVWLTSAHDAFVFIAVNTALAFCSTVPSGAVYAAIAESLPKASRAKT
ECHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHH
FALVYALPVTFLGGSTQFVITWLLKVTGEPMAVAWYMLGAALLALAGMVLVRESAPSRLR
HHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
ASPA
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]