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The map label for this gene is paaF [C]

Identifier: 87200110

GI number: 87200110

Start: 2230227

End: 2231000

Strand: Direct

Name: paaF [C]

Synonym: Saro_2094

Alternate gene names: 87200110

Gene position: 2230227-2231000 (Clockwise)

Preceding gene: 87200109

Following gene: 87200111

Centisome position: 62.62

GC content: 69.9

Gene sequence:

>774_bases
ATGACAGACCTGGTCCTGAAGACGCGGCACGAAAACGGCCGCATCGACCTCACTCTCAATCGCCCGGACAAGTTCAACGC
CCTGTCCGAAGCGCTCCTCGCCGCGCTCGGGTCGGAACTCGCCCGGATCGCCGCAGACCCCGGCGCCCGCGTCGTGGTCC
TCTCCGGCGCCGGCAAGGCGTTCTGCGCCGGCCACGATCTCGCAGAGATGCAGGCGGACCGCCGCCTTGATGCCTACCGC
CGCCTCTTCTCCGCCTGCACCGAAGTCATGGAGGCGATCGCCGCCCTGCCCGTGCCGGTCATCGCCCGCATCCACGGCGT
CGCCACGGCCGCCGGTTGCCAGCTCGTCGGCGCCTGCGACCTTGCCGTCGCGTCGGAAACCGCGCGCTTTGCCGTCTCGG
GCATCAACGTCGGCCTGTTCTGCTCGACCCCCGCCGTAGAGCTGAGCCGCAACGTGCCCGCCAAGCGCGCGTTCGAAATG
CTGGTGACCGGCAAGTTCATCGGCGCCCGCACCGCATCCGACTGGGGCCTCGTCAACACCGTGGCCGCTCCCGAAGCGCT
GGACTCCGCCGTCGACGACCTCGCCGCCACGATCCTCGCCAAGAGCCCCGACGCCATCCGCCGGGGCAAGGCCCTCTTCC
ACGCCCAGCGCGAACGCCCCCGCGCCGAGGCCTATGCCCTCGCCTGCGAAGTCATGGCGCAGAACATGATGGACGAGAAC
ACCGACGAAGGCATCACCGCCTTCCTCGAAAAGCGCCCGCCCGTCTGGCGCTGA

Upstream 100 bases:

>100_bases
TGACCGCCGGAGGGTGAGCGACCATGCCGCTCACGCCCGTCACCCTCCGAACGCGGTTCACGCCCAGGCACGGAAAGCCG
AAGCACCAAAGGAGAGGCGA

Downstream 100 bases:

>100_bases
AGGCCCGGAAGCCGCGCCGCCCCCGAAACGGTCCCCGCCCCCGCATCCGGGGCGCCCCCGCCAAACCGCCAACGCCCTGC
CCGTCACCCCGGGCCCGCCC

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 257; Mature: 256

Protein sequence:

>257_residues
MTDLVLKTRHENGRIDLTLNRPDKFNALSEALLAALGSELARIAADPGARVVVLSGAGKAFCAGHDLAEMQADRRLDAYR
RLFSACTEVMEAIAALPVPVIARIHGVATAAGCQLVGACDLAVASETARFAVSGINVGLFCSTPAVELSRNVPAKRAFEM
LVTGKFIGARTASDWGLVNTVAAPEALDSAVDDLAATILAKSPDAIRRGKALFHAQRERPRAEAYALACEVMAQNMMDEN
TDEGITAFLEKRPPVWR

Sequences:

>Translated_257_residues
MTDLVLKTRHENGRIDLTLNRPDKFNALSEALLAALGSELARIAADPGARVVVLSGAGKAFCAGHDLAEMQADRRLDAYR
RLFSACTEVMEAIAALPVPVIARIHGVATAAGCQLVGACDLAVASETARFAVSGINVGLFCSTPAVELSRNVPAKRAFEM
LVTGKFIGARTASDWGLVNTVAAPEALDSAVDDLAATILAKSPDAIRRGKALFHAQRERPRAEAYALACEVMAQNMMDEN
TDEGITAFLEKRPPVWR
>Mature_256_residues
TDLVLKTRHENGRIDLTLNRPDKFNALSEALLAALGSELARIAADPGARVVVLSGAGKAFCAGHDLAEMQADRRLDAYRR
LFSACTEVMEAIAALPVPVIARIHGVATAAGCQLVGACDLAVASETARFAVSGINVGLFCSTPAVELSRNVPAKRAFEML
VTGKFIGARTASDWGLVNTVAAPEALDSAVDDLAATILAKSPDAIRRGKALFHAQRERPRAEAYALACEVMAQNMMDENT
DEGITAFLEKRPPVWR

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI31542718, Length=248, Percent_Identity=42.741935483871, Blast_Score=196, Evalue=1e-50,
Organism=Homo sapiens, GI194097323, Length=251, Percent_Identity=31.8725099601594, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI70995211, Length=223, Percent_Identity=31.8385650224215, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI4502327, Length=266, Percent_Identity=28.9473684210526, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI37594469, Length=218, Percent_Identity=25.6880733944954, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI37594471, Length=218, Percent_Identity=25.6880733944954, Blast_Score=68, Evalue=9e-12,
Organism=Escherichia coli, GI1787659, Length=258, Percent_Identity=32.5581395348837, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI221142681, Length=267, Percent_Identity=32.9588014981273, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI1788597, Length=252, Percent_Identity=30.5555555555556, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1787660, Length=269, Percent_Identity=29.368029739777, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI87082183, Length=250, Percent_Identity=27.2, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1790281, Length=183, Percent_Identity=27.3224043715847, Blast_Score=66, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17540306, Length=242, Percent_Identity=37.603305785124, Blast_Score=159, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI25145438, Length=241, Percent_Identity=29.045643153527, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17554946, Length=241, Percent_Identity=27.3858921161826, Blast_Score=95, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17536985, Length=220, Percent_Identity=29.0909090909091, Blast_Score=94, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17560910, Length=212, Percent_Identity=31.6037735849057, Blast_Score=88, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17534483, Length=250, Percent_Identity=28, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17540714, Length=205, Percent_Identity=29.2682926829268, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17535521, Length=256, Percent_Identity=25, Blast_Score=78, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI25141363, Length=175, Percent_Identity=26.8571428571429, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17549921, Length=185, Percent_Identity=28.1081081081081, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI19922422, Length=256, Percent_Identity=38.28125, Blast_Score=182, Evalue=1e-46,
Organism=Drosophila melanogaster, GI20129971, Length=239, Percent_Identity=30.9623430962343, Blast_Score=101, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24653477, Length=239, Percent_Identity=30.9623430962343, Blast_Score=101, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24653139, Length=247, Percent_Identity=29.5546558704453, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI21357171, Length=207, Percent_Identity=30.9178743961353, Blast_Score=78, Evalue=5e-15,
Organism=Drosophila melanogaster, GI19920382, Length=219, Percent_Identity=28.7671232876712, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24650670, Length=191, Percent_Identity=32.4607329842932, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 27372; Mature: 27241

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLVLKTRHENGRIDLTLNRPDKFNALSEALLAALGSELARIAADPGARVVVLSGAGKA
CCCCEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCE
FCAGHDLAEMQADRRLDAYRRLFSACTEVMEAIAALPVPVIARIHGVATAAGCQLVGACD
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEHHH
LAVASETARFAVSGINVGLFCSTPAVELSRNVPAKRAFEMLVTGKFIGARTASDWGLVNT
HHHHHHHHHEEEECCCEEEEECCCHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHH
VAAPEALDSAVDDLAATILAKSPDAIRRGKALFHAQRERPRAEAYALACEVMAQNMMDEN
HCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCC
TDEGITAFLEKRPPVWR
CCHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TDLVLKTRHENGRIDLTLNRPDKFNALSEALLAALGSELARIAADPGARVVVLSGAGKA
CCCEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCE
FCAGHDLAEMQADRRLDAYRRLFSACTEVMEAIAALPVPVIARIHGVATAAGCQLVGACD
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEHHH
LAVASETARFAVSGINVGLFCSTPAVELSRNVPAKRAFEMLVTGKFIGARTASDWGLVNT
HHHHHHHHHEEEECCCEEEEECCCHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHH
VAAPEALDSAVDDLAATILAKSPDAIRRGKALFHAQRERPRAEAYALACEVMAQNMMDEN
HCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCC
TDEGITAFLEKRPPVWR
CCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA