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The map label for this gene is gyrB [H]

Identifier: 87200102

GI number: 87200102

Start: 2223045

End: 2225045

Strand: Direct

Name: gyrB [H]

Synonym: Saro_2086

Alternate gene names: 87200102

Gene position: 2223045-2225045 (Clockwise)

Preceding gene: 87200101

Following gene: 87200106

Centisome position: 62.42

GC content: 66.02

Gene sequence:

>2001_bases
ATGTCCGACGACCTTTTCGACAAGCTCCCCGCCACCAGCGCCGCCGAAGCCTATGACGGTTCCGCGATCGAGGTTCTCGA
AGGCCTCGAACCGGTCCGCCGCCGCCCCGGCATGTACATCGGCGGCACGGACGAACGCGCGCTGCACCACCTCGCCGCAG
AAGTGCTCGATAACGCGATGGACGAAGCGGTCGCTGGCCACGCCAACCGCATCGAGGTCCTGCTCGAGGAAGGCAACCGG
CTGACCATCTCCGACAACGGTCGCGGCATCCCGGTCGACGAACATCCCAAGTATCCGGGCAAGTCGGCACTCGAAGTGAT
CCTCACCACGCTCCACTCGGGTGGCAAGTTCTCGGGCAAGGCCTATGCAACCTCGGGCGGCCTCCACGGCGTCGGCGTCT
CGGTCGTCAACGCGCTGTCCTCGCTCACCCGCGTCGAAGTAGCCCGCAACAAGGAACTCTACGCGCAGGAATTTTCGCGC
GGGAACCCGACGACGAAGCTGATGAAGGTCGGCAACGCCCCCAACCGGCGCGGCACCCAGGTCACCTTCATCCCCGACAC
CGAGATCTTCGGAGAGGACGCGAAGTTCAAGCCTGCGCGCCTGTTCCGGCTGGTCCGCTCCAAGGCCTACCTCTTCGCCG
GCGTCGAGATCCGCTGGAAGTGCGAGCCCTCGCTCGCCAGCGACGACGTGCCCGCCGAAGCCGTGTTCCAGTTCCCCGGC
GGCCTGGCCGATCATCTGGCCGAACAGGTCTCGGGCCGCGAATGCGTCACTTCGGTCCCCTTCGCGGGTCGCCAGGATTT
CCCGCTCGGTCCCGATGGCGAGGAAATGGGCCGCGTCGAATGGGCGATTGCCTGGCCGCTGTGGTCCGACGGCTCCTACT
CGTGGTACTGCAATACCATTCCCACGCCCGATGGCGGCACCCATGAACAGGGCCTGCGCGCGGCACTGACCAAGGGCATC
CGCGCCTTCGCGGACCTCATCGGCCAGAAAAAGGCCAAGGACATCGCGCCGGAAGACATGATCACCGGCAGCGAGATCAT
GCTCTCGGTCTTCATCCGCGATCCCCAGTTCCAGAGCCAGACCAAGGACCGCCTGACCAGCCCCGAAGCCGCGCGCATGG
TCGAGGCCGCCGTGCGCGACCATTTCGACCACTTCCTCACCGACAACATGGAGCGCGGCAAGGCCCTGCTCGGCGCGGTG
ATGGAGCGCATGGACGAACGCCTGCGCCGCAAGGCGGAGCGTGAGGTCAAGCGCAAGACCGCGACCAACGCCCGCAAGCT
GCGCCTCCCCGGCAAGCTCACCGACTGCTCGGGCGAAGGCAGCGGAGAGACCGAACTGTTCATCGTCGAAGGCGATTCGG
CAGGCGGCAGCGCCAAGCAGGCACGCGACCGCAAGACCCAGGCGATCCTGCCGATCCGCGGCAAGATCCTCAACGTCGCA
AGCGCCACCGCCGACAAGATCCGCGCCAACCAGGAAATCGCCGACCTCGCGCTCGCACTCGGCTGCGGCATGCGCAAGGA
CTGCAATCCCGATGCTCTACGCTATGACCGCGTCATCATCATGACCGACGCCGACGTCGACGGCGCACACATCGCCACGC
TGCTGATGACGTTCTTCTTCCAGGAGATGCCCGAGCTCGTCAGGCGCGGTCATCTCTACCTCGCCCAGCCGCCGCTCTAC
CGCCTGACCAGCGGCAGCACGTCGGCCTACGCCAAGGACGACGCCCACCGCGCCGAACTCGAAGCCACGAAGTTCAAGGG
CAAGAAGGTCGAAGTCGGGCGCTTCAAGGGCCTCGGCGAAATGAACCCTCAGCAGTTGCGCGAAACAACCATGGCGCCTG
CCACCCGCAGCCTCATCCGCATCACCCTGCCGCCCGAGTACGAAGGCCGCGCGGCAGTGAAGGACCTGGTCGACAGGCTG
ATGGGCCGCGACCCGGCACAACGCTTCATGTTCATCCAGAACCGCGCGAGCGAGATCGATCCGGAACTGATCGACGCGTG
A

Upstream 100 bases:

>100_bases
GCTCCTGCAAAGTTCTGAACGCCCGAAAGCGGGTGATTTCGCCGGATAAGCCGGCATCCCCGCTTGCGCCCGGATTCGCG
CGCGTTCTATACCCGTTCGC

Downstream 100 bases:

>100_bases
TGATGGTGCGCGAGGACAGCGAGGCGGGCCTCTTGAATGGCTGGCGACTTCGGCTGCTTGACTGTTTTTCAAAACACTGA
GGCGGTTTTCCGCGAGCCGC

Product: DNA topoisomerase IV subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 666; Mature: 665

Protein sequence:

>666_residues
MSDDLFDKLPATSAAEAYDGSAIEVLEGLEPVRRRPGMYIGGTDERALHHLAAEVLDNAMDEAVAGHANRIEVLLEEGNR
LTISDNGRGIPVDEHPKYPGKSALEVILTTLHSGGKFSGKAYATSGGLHGVGVSVVNALSSLTRVEVARNKELYAQEFSR
GNPTTKLMKVGNAPNRRGTQVTFIPDTEIFGEDAKFKPARLFRLVRSKAYLFAGVEIRWKCEPSLASDDVPAEAVFQFPG
GLADHLAEQVSGRECVTSVPFAGRQDFPLGPDGEEMGRVEWAIAWPLWSDGSYSWYCNTIPTPDGGTHEQGLRAALTKGI
RAFADLIGQKKAKDIAPEDMITGSEIMLSVFIRDPQFQSQTKDRLTSPEAARMVEAAVRDHFDHFLTDNMERGKALLGAV
MERMDERLRRKAEREVKRKTATNARKLRLPGKLTDCSGEGSGETELFIVEGDSAGGSAKQARDRKTQAILPIRGKILNVA
SATADKIRANQEIADLALALGCGMRKDCNPDALRYDRVIIMTDADVDGAHIATLLMTFFFQEMPELVRRGHLYLAQPPLY
RLTSGSTSAYAKDDAHRAELEATKFKGKKVEVGRFKGLGEMNPQQLRETTMAPATRSLIRITLPPEYEGRAAVKDLVDRL
MGRDPAQRFMFIQNRASEIDPELIDA

Sequences:

>Translated_666_residues
MSDDLFDKLPATSAAEAYDGSAIEVLEGLEPVRRRPGMYIGGTDERALHHLAAEVLDNAMDEAVAGHANRIEVLLEEGNR
LTISDNGRGIPVDEHPKYPGKSALEVILTTLHSGGKFSGKAYATSGGLHGVGVSVVNALSSLTRVEVARNKELYAQEFSR
GNPTTKLMKVGNAPNRRGTQVTFIPDTEIFGEDAKFKPARLFRLVRSKAYLFAGVEIRWKCEPSLASDDVPAEAVFQFPG
GLADHLAEQVSGRECVTSVPFAGRQDFPLGPDGEEMGRVEWAIAWPLWSDGSYSWYCNTIPTPDGGTHEQGLRAALTKGI
RAFADLIGQKKAKDIAPEDMITGSEIMLSVFIRDPQFQSQTKDRLTSPEAARMVEAAVRDHFDHFLTDNMERGKALLGAV
MERMDERLRRKAEREVKRKTATNARKLRLPGKLTDCSGEGSGETELFIVEGDSAGGSAKQARDRKTQAILPIRGKILNVA
SATADKIRANQEIADLALALGCGMRKDCNPDALRYDRVIIMTDADVDGAHIATLLMTFFFQEMPELVRRGHLYLAQPPLY
RLTSGSTSAYAKDDAHRAELEATKFKGKKVEVGRFKGLGEMNPQQLRETTMAPATRSLIRITLPPEYEGRAAVKDLVDRL
MGRDPAQRFMFIQNRASEIDPELIDA
>Mature_665_residues
SDDLFDKLPATSAAEAYDGSAIEVLEGLEPVRRRPGMYIGGTDERALHHLAAEVLDNAMDEAVAGHANRIEVLLEEGNRL
TISDNGRGIPVDEHPKYPGKSALEVILTTLHSGGKFSGKAYATSGGLHGVGVSVVNALSSLTRVEVARNKELYAQEFSRG
NPTTKLMKVGNAPNRRGTQVTFIPDTEIFGEDAKFKPARLFRLVRSKAYLFAGVEIRWKCEPSLASDDVPAEAVFQFPGG
LADHLAEQVSGRECVTSVPFAGRQDFPLGPDGEEMGRVEWAIAWPLWSDGSYSWYCNTIPTPDGGTHEQGLRAALTKGIR
AFADLIGQKKAKDIAPEDMITGSEIMLSVFIRDPQFQSQTKDRLTSPEAARMVEAAVRDHFDHFLTDNMERGKALLGAVM
ERMDERLRRKAEREVKRKTATNARKLRLPGKLTDCSGEGSGETELFIVEGDSAGGSAKQARDRKTQAILPIRGKILNVAS
ATADKIRANQEIADLALALGCGMRKDCNPDALRYDRVIIMTDADVDGAHIATLLMTFFFQEMPELVRRGHLYLAQPPLYR
LTSGSTSAYAKDDAHRAELEATKFKGKKVEVGRFKGLGEMNPQQLRETTMAPATRSLIRITLPPEYEGRAAVKDLVDRLM
GRDPAQRFMFIQNRASEIDPELIDA

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=628, Percent_Identity=24.3630573248408, Blast_Score=127, Evalue=4e-29,
Organism=Homo sapiens, GI19913408, Length=633, Percent_Identity=23.696682464455, Blast_Score=121, Evalue=2e-27,
Organism=Escherichia coli, GI1789408, Length=632, Percent_Identity=43.3544303797468, Blast_Score=461, Evalue=1e-131,
Organism=Escherichia coli, GI48994957, Length=574, Percent_Identity=43.7282229965157, Blast_Score=422, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI17535065, Length=643, Percent_Identity=23.6391912908243, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI212645845, Length=577, Percent_Identity=23.2235701906412, Blast_Score=109, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6324241, Length=653, Percent_Identity=25.2679938744257, Blast_Score=116, Evalue=1e-26,
Organism=Drosophila melanogaster, GI17136538, Length=645, Percent_Identity=23.2558139534884, Blast_Score=119, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR005737 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 73184; Mature: 73053

Theoretical pI: Translated: 6.47; Mature: 6.47

Prosite motif: PS00626 RCC1_2 ; PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDDLFDKLPATSAAEAYDGSAIEVLEGLEPVRRRPGMYIGGTDERALHHLAAEVLDNAM
CCCHHHHHCCCCCCHHHCCCCHHHHHHCCHHHHHCCCEEECCCHHHHHHHHHHHHHHHHH
DEAVAGHANRIEVLLEEGNRLTISDNGRGIPVDEHPKYPGKSALEVILTTLHSGGKFSGK
HHHHCCCCHHEEEEEECCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCC
AYATSGGLHGVGVSVVNALSSLTRVEVARNKELYAQEFSRGNPTTKLMKVGNAPNRRGTQ
EEEECCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCHHHEEECCCCCCCCCE
VTFIPDTEIFGEDAKFKPARLFRLVRSKAYLFAGVEIRWKCEPSLASDDVPAEAVFQFPG
EEEECCHHHCCCCCCCCHHHHHHHHHHHHEEEECEEEEEEECCCCCCCCCCHHHHHHCCC
GLADHLAEQVSGRECVTSVPFAGRQDFPLGPDGEEMGRVEWAIAWPLWSDGSYSWYCNTI
CHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCHHHHCCEEEEEEEEEECCCCCEEEECCC
PTPDGGTHEQGLRAALTKGIRAFADLIGQKKAKDIAPEDMITGSEIMLSVFIRDPQFQSQ
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCHHHCCCHHHEEEEEECCCCHHHH
TKDRLTSPEAARMVEAAVRDHFDHFLTDNMERGKALLGAVMERMDERLRRKAEREVKRKT
HHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATNARKLRLPGKLTDCSGEGSGETELFIVEGDSAGGSAKQARDRKTQAILPIRGKILNVA
HCCCCEEECCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHCEECCCCCCCHHHH
SATADKIRANQEIADLALALGCGMRKDCNPDALRYDRVIIMTDADVDGAHIATLLMTFFF
HHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCEEECEEEEEECCCCCHHHHHHHHHHHHH
QEMPELVRRGHLYLAQPPLYRLTSGSTSAYAKDDAHRAELEATKFKGKKVEVGRFKGLGE
HHHHHHHHCCCEEEECCCCEEECCCCCCCCCCCCHHHHHHHHHHCCCCEEECHHCCCCCC
MNPQQLRETTMAPATRSLIRITLPPEYEGRAAVKDLVDRLMGRDPAQRFMFIQNRASEID
CCHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCC
PELIDA
HHHCCC
>Mature Secondary Structure 
SDDLFDKLPATSAAEAYDGSAIEVLEGLEPVRRRPGMYIGGTDERALHHLAAEVLDNAM
CCHHHHHCCCCCCHHHCCCCHHHHHHCCHHHHHCCCEEECCCHHHHHHHHHHHHHHHHH
DEAVAGHANRIEVLLEEGNRLTISDNGRGIPVDEHPKYPGKSALEVILTTLHSGGKFSGK
HHHHCCCCHHEEEEEECCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCC
AYATSGGLHGVGVSVVNALSSLTRVEVARNKELYAQEFSRGNPTTKLMKVGNAPNRRGTQ
EEEECCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCHHHEEECCCCCCCCCE
VTFIPDTEIFGEDAKFKPARLFRLVRSKAYLFAGVEIRWKCEPSLASDDVPAEAVFQFPG
EEEECCHHHCCCCCCCCHHHHHHHHHHHHEEEECEEEEEEECCCCCCCCCCHHHHHHCCC
GLADHLAEQVSGRECVTSVPFAGRQDFPLGPDGEEMGRVEWAIAWPLWSDGSYSWYCNTI
CHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCHHHHCCEEEEEEEEEECCCCCEEEECCC
PTPDGGTHEQGLRAALTKGIRAFADLIGQKKAKDIAPEDMITGSEIMLSVFIRDPQFQSQ
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCHHHCCCHHHEEEEEECCCCHHHH
TKDRLTSPEAARMVEAAVRDHFDHFLTDNMERGKALLGAVMERMDERLRRKAEREVKRKT
HHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATNARKLRLPGKLTDCSGEGSGETELFIVEGDSAGGSAKQARDRKTQAILPIRGKILNVA
HCCCCEEECCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHCEECCCCCCCHHHH
SATADKIRANQEIADLALALGCGMRKDCNPDALRYDRVIIMTDADVDGAHIATLLMTFFF
HHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCEEECEEEEEECCCCCHHHHHHHHHHHHH
QEMPELVRRGHLYLAQPPLYRLTSGSTSAYAKDDAHRAELEATKFKGKKVEVGRFKGLGE
HHHHHHHHCCCEEEECCCCEEECCCCCCCCCCCCHHHHHHHHHHCCCCEEECHHCCCCCC
MNPQQLRETTMAPATRSLIRITLPPEYEGRAAVKDLVDRLMGRDPAQRFMFIQNRASEID
CCHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCC
PELIDA
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9797224 [H]