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The map label for this gene is ntrX [H]

Identifier: 87199947

GI number: 87199947

Start: 2043837

End: 2045222

Strand: Direct

Name: ntrX [H]

Synonym: Saro_1930

Alternate gene names: 87199947

Gene position: 2043837-2045222 (Clockwise)

Preceding gene: 87199946

Following gene: 87199948

Centisome position: 57.39

GC content: 63.85

Gene sequence:

>1386_bases
ATGGCACTCGATATCCTCATCGTCGATGACGAACGCGACATCCGCGAACTGGTAGCGGGGGTGCTGAGCGACGAAGGCTA
TGGCTGTCGCTTGGCCGCCGACAGCACTTCCGCGCTTGCCGCCATCGACGAACGCCGCCCCAGCCTTGTTCTGCTCGACG
TCTGGCTTCACGGCAGCCCGATGGATGGGCTCGAAGTGCTCGACGAGATCAAGAAGCGCGAGCCGGAACTCCCGGTCATC
ATCTTCTCGGGCCACGGCAATATCGACACTGCGGTCTCAGCGATCAGCCGCGGTGCGATGGATTTCCTCGAAAAGCCCTT
CGAAGCGGAGCGCCTCCTGCTGCTCGTCGAGCGTGCAACCGAAACCGAGCGCCTTCGGCGCGAAAACCAGCGCCTGCGCC
AGGGCTTCGCCATGGCCGAGGAGTTCACCGGCAACAGTTCGGCCATCAACCAGGTACGCGCCACGCTTAAGCGCGTCGCC
AACACTGGCAGCCGCCTGCTCATCTCGGGCCCGGCCGGGTCGGGCAAGGAAGTGGCCGCGCGTCTCCTCCATTCCTGGAG
CCCGCGCGCGCAGAGCGCCTTCGTAACGGTCAACTCGGCCCGCATCACGCCGGAACGCTTCGAACAGGAACTCTTCGGAG
AGGAGGTCGATGGCAAGCTGGTGAGGGCAGGGCTCCTCGAAATGGCCGATGGCGGGACGCTGTTCCTCGACGAAGTATCC
GACATGCCCGCATCGAGCCAGGCCCGCATCCTGCGCGTCCTGACCGAACAGTCCTTCGTCAGAGTCGGAGGCCACCGTCA
ACTGCGCGTCGACGTGCGGGTGGTTTCATCGTCATCACGTCCGCTCGAGAAGGAGATTGCCGAGCGCCGTTTCCGCGAAG
ACCTGTATTATCGTTTGAACGTCGTGCCCGTGGCGATTCCCTCGCTCATGGAGCGGCGCGACGACATTCCCGCGCTGGTC
GATCATTTCTTTGCCCGCTACGCCAACGAGCAAGGGGTGCCGCCGCCCGCCGTCTCCCCTGAAGCAATGGCCGCGATGCA
GAGCTATGATTGGCCGGGCAACGTCCGCCAGCTCCGCAACGTGGTCGAGCGCACCGTCATTCTTGCCCCGCGTGATCGGC
TTGCACGGATCGACGCGGACATGCTCCCTTCCGAGATCGTCAGCACTCCGATCGGCGGCGACACCGGTGGCTCCGCGCTC
ATGGGCGTGCCCCTGCGCGAAGCGCGCGAGAACTTCGAGCGCGAATATCTGAAGGTCCAGATCCGCCGCTTTTCCGGCAA
CATTTCCAAGACCGCCAGTTTCATCGGGATGGAACGTTCAGCCCTTCATCGCAAGCTCAAGCTGCTCGGCATGGCCGAAC
GGCGAGAAGGTGAAGACGAGGAATGA

Upstream 100 bases:

>100_bases
GCCGAGCCCGGCGGCGGCACCCGCGTAACAATGCGCTTCGCCCGCGATCCGCTTGCAACTTCCGCCCACCACGCCGAACA
GGACTGACAGGACCCTCACG

Downstream 100 bases:

>100_bases
TGCTGCAAGGCGGCTCGTGAATACGCATTAAATCGGAACCCTGTGCAATTTCCCATGTTTACGGGACGGGCGCGGGGACA
TAAACTCGTCAACGAACCCG

Product: two component sigma-54 specific Fis family transcriptional regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 460

Protein sequence:

>461_residues
MALDILIVDDERDIRELVAGVLSDEGYGCRLAADSTSALAAIDERRPSLVLLDVWLHGSPMDGLEVLDEIKKREPELPVI
IFSGHGNIDTAVSAISRGAMDFLEKPFEAERLLLLVERATETERLRRENQRLRQGFAMAEEFTGNSSAINQVRATLKRVA
NTGSRLLISGPAGSGKEVAARLLHSWSPRAQSAFVTVNSARITPERFEQELFGEEVDGKLVRAGLLEMADGGTLFLDEVS
DMPASSQARILRVLTEQSFVRVGGHRQLRVDVRVVSSSSRPLEKEIAERRFREDLYYRLNVVPVAIPSLMERRDDIPALV
DHFFARYANEQGVPPPAVSPEAMAAMQSYDWPGNVRQLRNVVERTVILAPRDRLARIDADMLPSEIVSTPIGGDTGGSAL
MGVPLREARENFEREYLKVQIRRFSGNISKTASFIGMERSALHRKLKLLGMAERREGEDEE

Sequences:

>Translated_461_residues
MALDILIVDDERDIRELVAGVLSDEGYGCRLAADSTSALAAIDERRPSLVLLDVWLHGSPMDGLEVLDEIKKREPELPVI
IFSGHGNIDTAVSAISRGAMDFLEKPFEAERLLLLVERATETERLRRENQRLRQGFAMAEEFTGNSSAINQVRATLKRVA
NTGSRLLISGPAGSGKEVAARLLHSWSPRAQSAFVTVNSARITPERFEQELFGEEVDGKLVRAGLLEMADGGTLFLDEVS
DMPASSQARILRVLTEQSFVRVGGHRQLRVDVRVVSSSSRPLEKEIAERRFREDLYYRLNVVPVAIPSLMERRDDIPALV
DHFFARYANEQGVPPPAVSPEAMAAMQSYDWPGNVRQLRNVVERTVILAPRDRLARIDADMLPSEIVSTPIGGDTGGSAL
MGVPLREARENFEREYLKVQIRRFSGNISKTASFIGMERSALHRKLKLLGMAERREGEDEE
>Mature_460_residues
ALDILIVDDERDIRELVAGVLSDEGYGCRLAADSTSALAAIDERRPSLVLLDVWLHGSPMDGLEVLDEIKKREPELPVII
FSGHGNIDTAVSAISRGAMDFLEKPFEAERLLLLVERATETERLRRENQRLRQGFAMAEEFTGNSSAINQVRATLKRVAN
TGSRLLISGPAGSGKEVAARLLHSWSPRAQSAFVTVNSARITPERFEQELFGEEVDGKLVRAGLLEMADGGTLFLDEVSD
MPASSQARILRVLTEQSFVRVGGHRQLRVDVRVVSSSSRPLEKEIAERRFREDLYYRLNVVPVAIPSLMERRDDIPALVD
HFFARYANEQGVPPPAVSPEAMAAMQSYDWPGNVRQLRNVVERTVILAPRDRLARIDADMLPSEIVSTPIGGDTGGSALM
GVPLREARENFEREYLKVQIRRFSGNISKTASFIGMERSALHRKLKLLGMAERREGEDEE

Specific function: Member of the two-component regulatory system ntrY/ntrX involved in the activation of nitrogen assimilatory genes such as glnA. NtrX is probably phosphorylated by ntrY and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=469, Percent_Identity=37.1002132196162, Blast_Score=295, Evalue=4e-81,
Organism=Escherichia coli, GI1788550, Length=456, Percent_Identity=33.9912280701754, Blast_Score=285, Evalue=4e-78,
Organism=Escherichia coli, GI1790437, Length=456, Percent_Identity=35.9649122807018, Blast_Score=267, Evalue=1e-72,
Organism=Escherichia coli, GI1788905, Length=447, Percent_Identity=34.2281879194631, Blast_Score=242, Evalue=5e-65,
Organism=Escherichia coli, GI87082117, Length=346, Percent_Identity=36.4161849710983, Blast_Score=196, Evalue=3e-51,
Organism=Escherichia coli, GI1789233, Length=329, Percent_Identity=32.5227963525836, Blast_Score=191, Evalue=1e-49,
Organism=Escherichia coli, GI1789087, Length=276, Percent_Identity=38.4057971014493, Blast_Score=186, Evalue=3e-48,
Organism=Escherichia coli, GI87082152, Length=323, Percent_Identity=35.6037151702786, Blast_Score=186, Evalue=3e-48,
Organism=Escherichia coli, GI1787583, Length=342, Percent_Identity=33.9181286549708, Blast_Score=184, Evalue=8e-48,
Organism=Escherichia coli, GI1786524, Length=324, Percent_Identity=34.2592592592593, Blast_Score=169, Evalue=2e-43,
Organism=Escherichia coli, GI87081872, Length=327, Percent_Identity=32.1100917431193, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1789828, Length=235, Percent_Identity=34.468085106383, Blast_Score=115, Evalue=6e-27,
Organism=Escherichia coli, GI87081858, Length=296, Percent_Identity=27.7027027027027, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI1786599, Length=115, Percent_Identity=33.9130434782609, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 51325; Mature: 51194

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALDILIVDDERDIRELVAGVLSDEGYGCRLAADSTSALAAIDERRPSLVLLDVWLHGSP
CEEEEEEECCCHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHCCCCCEEEEEEEECCCC
MDGLEVLDEIKKREPELPVIIFSGHGNIDTAVSAISRGAMDFLEKPFEAERLLLLVERAT
CCHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
ETERLRRENQRLRQGFAMAEEFTGNSSAINQVRATLKRVANTGSRLLISGPAGSGKEVAA
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHH
RLLHSWSPRAQSAFVTVNSARITPERFEQELFGEEVDGKLVRAGLLEMADGGTLFLDEVS
HHHHCCCCCCCEEEEEEECCCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEEEECCC
DMPASSQARILRVLTEQSFVRVGGHRQLRVDVRVVSSSSRPLEKEIAERRFREDLYYRLN
CCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHEEEE
VVPVAIPSLMERRDDIPALVDHFFARYANEQGVPPPAVSPEAMAAMQSYDWPGNVRQLRN
EHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHH
VVERTVILAPRDRLARIDADMLPSEIVSTPIGGDTGGSALMGVPLREARENFEREYLKVQ
HHHHHEEECCCHHHHHCCHHHCCHHHHCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHH
IRRFSGNISKTASFIGMERSALHRKLKLLGMAERREGEDEE
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
ALDILIVDDERDIRELVAGVLSDEGYGCRLAADSTSALAAIDERRPSLVLLDVWLHGSP
EEEEEEECCCHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHCCCCCEEEEEEEECCCC
MDGLEVLDEIKKREPELPVIIFSGHGNIDTAVSAISRGAMDFLEKPFEAERLLLLVERAT
CCHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
ETERLRRENQRLRQGFAMAEEFTGNSSAINQVRATLKRVANTGSRLLISGPAGSGKEVAA
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHH
RLLHSWSPRAQSAFVTVNSARITPERFEQELFGEEVDGKLVRAGLLEMADGGTLFLDEVS
HHHHCCCCCCCEEEEEEECCCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEEEECCC
DMPASSQARILRVLTEQSFVRVGGHRQLRVDVRVVSSSSRPLEKEIAERRFREDLYYRLN
CCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHEEEE
VVPVAIPSLMERRDDIPALVDHFFARYANEQGVPPPAVSPEAMAAMQSYDWPGNVRQLRN
EHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHH
VVERTVILAPRDRLARIDADMLPSEIVSTPIGGDTGGSALMGVPLREARENFEREYLKVQ
HHHHHEEECCCHHHHHCCHHHCCHHHHCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHH
IRRFSGNISKTASFIGMERSALHRKLKLLGMAERREGEDEE
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1661370 [H]