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The map label for this gene is amy [H]

Identifier: 87199907

GI number: 87199907

Start: 1999508

End: 2001337

Strand: Direct

Name: amy [H]

Synonym: Saro_1890

Alternate gene names: 87199907

Gene position: 1999508-2001337 (Clockwise)

Preceding gene: 87199906

Following gene: 87199908

Centisome position: 56.14

GC content: 63.93

Gene sequence:

>1830_bases
ATGCGTAGGATATCCCTGATTTCCGCGCTCGCTCTGGTCCTGTCCTTGGGGCAGCCGGCGTTGGCCGACGTGCCACTGGC
CGAAGTGCGTGCGCGTCCCGCTGCAGGCGAAGTGGTCTATTTCCTGCTGCCCGACCGGTTCGATAACGGCGACAGGAAGA
ACGACCTTGGCGGGCTCAAGGGCGATCGGCTGAAGACCGGGTTCGACCCGTCGGCAAAGGGGTTTTATCACGGCGGGGAC
ATCAAGGGCCTGCTCTCGCGGCTCGACTACCTCCAGGACATGGGCGTGACGGCGCTGTGGGTGGCGCCGGTGTTCCGCAA
CAAGCCGGTACAGGGCGCGCCCGGGCAGGAGAGCGCGGGGTATCACGGATACTGGGTAACCGATTTCACCCGGATCGATC
CGCACTTCGGCAGCAATGCCGATTTCAAGGCGCTGGTCGACGCTGCCCATGCTCGCGGCATCAAGGTCTACATGGACATC
ATCGCCAATCACACGGCGGACGTGATCCACTATGCCGATGGCGATTACTCATACCGCAACCGGGCCGACTGGCCCTATTC
GCGCAAGGGTGGATTGTCCGGCAAGGCGATCAACCAGGGGTTTGCGGGCGACGAGGACTCGAGCGCGGCGAACTTTGCGA
AGCTGACCGATCCCGGCGCGGCATATCAGCCGTTGATCGACCCGGCCGAGCGAAACGTGAAGGTCCCGGCCTGGCTGAAC
GATCCGATCTACTACCATAATCGCGGCAACACGACGTTCACCGGCGAGGACAGCCGGTTCGGGGACTTTGCCGGACTGGA
CGACCTGTTCACCGAACATCCCCGCGTTCGTGCCGGGATGGTTGAGATCTATGCCGACTGGATCCGCAGGTTCGGCATCG
ACGGCTACCGCGTCGATACGGCCAAGCATGTCGATCCGGGCTTCTGGCAAGTGTTCACTCCGGAAATTCGCAAGGTCGCG
GCAGAGGCCGGCATACCGAATTTCGCGGTGTTCGCTGAAATCGCCAACGGCGGCTCGGACCCCGGCACGATCGCGCGACA
CACCCGCCGCGACGGCTTTCCGCAAGTGCTCGACTTCGCGTTCCAGGAAGCGGTGCGGGACCTTGTCGGCAAGGGTAAGG
CGACGCGCGTGCTGGCAGAGACCTTTGACGGCGACGTACTCTATGAAGGTGGCGAGGCGGCGGCGCTCGCCATGCCAACT
TTCCTCGGCAACCACGACATGGGCCGTTTCTCGACCGCAGTCCGGCAGGACCGCCCGGGCATTTCCGACAAGGAACTGCT
CGACCGCGTCGCGCTGGCGCACGTCATGCTGATGACCCTTCGCGGTTCGCCGGTGATCTACTACGGCGACGAGCAGGGCT
TTGTCGGCGACGGCGGCGACCAGGACGCGCGCGAGGACATGTTCCCGAGCCGCACGGCCAGCTACAACGACAATCGCCTG
ATCGGGACGGCCAGCACGACGGCTGCCGACAATTTCGACGAGGGGCATCCCCTCTATCGGCTGATCCGCGATCTTGCCGT
ACTGCGGAAGGCGCATCCCGCGCTGGCCCGGGGGCGGCAGGTTACGCGAACGTATTCGGAAGGGCCCGGACTGTTTTCCG
TCTCCCGCTTCGATCCGGAGACGGGTACGGAATACCTGATAGCGTTCAACACATCGGACAAGCCACTTCGTGCAACCAGC
GTGATTGGCGCGTCGGCAAGCGGTCTCGAAGGGCTCTACGGCCAATGTCCGTTATCGGTGGCGGCGCCGGGTTCGGTGCT
GCTCGATCTCCCCGCATTTGGCAGCGTCGTCTGTCGTGTCATCCTCTCCCCGGAATCCAGCGTTCGATGA

Upstream 100 bases:

>100_bases
GCGTTCCTCGAAACCGTCAGCGAAGCCTACAGAAAGGACGTTGCGCGAATGCCCCTTCACGCCGACTACCTCGAACATGT
CTGCGGAAAGGTGGCTGCCC

Downstream 100 bases:

>100_bases
AGCAGACCGAAACAGCCATTGCCGACCCCAAAATGCACACGGAGACGCCGTGGTGGCGCGGTGCGGCGATCTATCAGATC
TATCCACGCAGCTTTTGCGA

Product: alpha amylase catalytic subunit

Products: NA

Alternate protein names: 1,4-alpha-D-glucan glucanohydrolase [H]

Number of amino acids: Translated: 609; Mature: 609

Protein sequence:

>609_residues
MRRISLISALALVLSLGQPALADVPLAEVRARPAAGEVVYFLLPDRFDNGDRKNDLGGLKGDRLKTGFDPSAKGFYHGGD
IKGLLSRLDYLQDMGVTALWVAPVFRNKPVQGAPGQESAGYHGYWVTDFTRIDPHFGSNADFKALVDAAHARGIKVYMDI
IANHTADVIHYADGDYSYRNRADWPYSRKGGLSGKAINQGFAGDEDSSAANFAKLTDPGAAYQPLIDPAERNVKVPAWLN
DPIYYHNRGNTTFTGEDSRFGDFAGLDDLFTEHPRVRAGMVEIYADWIRRFGIDGYRVDTAKHVDPGFWQVFTPEIRKVA
AEAGIPNFAVFAEIANGGSDPGTIARHTRRDGFPQVLDFAFQEAVRDLVGKGKATRVLAETFDGDVLYEGGEAAALAMPT
FLGNHDMGRFSTAVRQDRPGISDKELLDRVALAHVMLMTLRGSPVIYYGDEQGFVGDGGDQDAREDMFPSRTASYNDNRL
IGTASTTAADNFDEGHPLYRLIRDLAVLRKAHPALARGRQVTRTYSEGPGLFSVSRFDPETGTEYLIAFNTSDKPLRATS
VIGASASGLEGLYGQCPLSVAAPGSVLLDLPAFGSVVCRVILSPESSVR

Sequences:

>Translated_609_residues
MRRISLISALALVLSLGQPALADVPLAEVRARPAAGEVVYFLLPDRFDNGDRKNDLGGLKGDRLKTGFDPSAKGFYHGGD
IKGLLSRLDYLQDMGVTALWVAPVFRNKPVQGAPGQESAGYHGYWVTDFTRIDPHFGSNADFKALVDAAHARGIKVYMDI
IANHTADVIHYADGDYSYRNRADWPYSRKGGLSGKAINQGFAGDEDSSAANFAKLTDPGAAYQPLIDPAERNVKVPAWLN
DPIYYHNRGNTTFTGEDSRFGDFAGLDDLFTEHPRVRAGMVEIYADWIRRFGIDGYRVDTAKHVDPGFWQVFTPEIRKVA
AEAGIPNFAVFAEIANGGSDPGTIARHTRRDGFPQVLDFAFQEAVRDLVGKGKATRVLAETFDGDVLYEGGEAAALAMPT
FLGNHDMGRFSTAVRQDRPGISDKELLDRVALAHVMLMTLRGSPVIYYGDEQGFVGDGGDQDAREDMFPSRTASYNDNRL
IGTASTTAADNFDEGHPLYRLIRDLAVLRKAHPALARGRQVTRTYSEGPGLFSVSRFDPETGTEYLIAFNTSDKPLRATS
VIGASASGLEGLYGQCPLSVAAPGSVLLDLPAFGSVVCRVILSPESSVR
>Mature_609_residues
MRRISLISALALVLSLGQPALADVPLAEVRARPAAGEVVYFLLPDRFDNGDRKNDLGGLKGDRLKTGFDPSAKGFYHGGD
IKGLLSRLDYLQDMGVTALWVAPVFRNKPVQGAPGQESAGYHGYWVTDFTRIDPHFGSNADFKALVDAAHARGIKVYMDI
IANHTADVIHYADGDYSYRNRADWPYSRKGGLSGKAINQGFAGDEDSSAANFAKLTDPGAAYQPLIDPAERNVKVPAWLN
DPIYYHNRGNTTFTGEDSRFGDFAGLDDLFTEHPRVRAGMVEIYADWIRRFGIDGYRVDTAKHVDPGFWQVFTPEIRKVA
AEAGIPNFAVFAEIANGGSDPGTIARHTRRDGFPQVLDFAFQEAVRDLVGKGKATRVLAETFDGDVLYEGGEAAALAMPT
FLGNHDMGRFSTAVRQDRPGISDKELLDRVALAHVMLMTLRGSPVIYYGDEQGFVGDGGDQDAREDMFPSRTASYNDNRL
IGTASTTAADNFDEGHPLYRLIRDLAVLRKAHPALARGRQVTRTYSEGPGLFSVSRFDPETGTEYLIAFNTSDKPLRATS
VIGASASGLEGLYGQCPLSVAAPGSVLLDLPAFGSVVCRVILSPESSVR

Specific function: Since Only Maltooligosaccharides Up To A Chain Length Of 6 Glucose Units Are Actively Transported Through The Cytoplasmic Membrane VIa The Membrane-Bound Complex Of Three Proteins, Malf, Malg, And Malk, Longer Maltooligosaccharides Must First Be Degraded

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI1789995, Length=546, Percent_Identity=28.2051282051282, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI1790687, Length=88, Percent_Identity=37.5, Blast_Score=70, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6322245, Length=89, Percent_Identity=37.0786516853933, Blast_Score=73, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6319776, Length=88, Percent_Identity=35.2272727272727, Blast_Score=68, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6321731, Length=88, Percent_Identity=35.2272727272727, Blast_Score=68, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24586587, Length=415, Percent_Identity=23.1325301204819, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24583747, Length=533, Percent_Identity=25.140712945591, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24583749, Length=533, Percent_Identity=25.140712945591, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI221330053, Length=89, Percent_Identity=38.2022471910112, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24586597, Length=89, Percent_Identity=37.0786516853933, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24586599, Length=89, Percent_Identity=34.8314606741573, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24583745, Length=89, Percent_Identity=33.7078651685393, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24586591, Length=110, Percent_Identity=32.7272727272727, Blast_Score=69, Evalue=6e-12,
Organism=Drosophila melanogaster, GI45549022, Length=395, Percent_Identity=22.0253164556962, Blast_Score=68, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24586589, Length=124, Percent_Identity=33.8709677419355, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24586593, Length=93, Percent_Identity=34.4086021505376, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006048
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR005323 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02806 Alpha-amylase_C; PF03714 PUD [H]

EC number: =3.2.1.1 [H]

Molecular weight: Translated: 66228; Mature: 66228

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRISLISALALVLSLGQPALADVPLAEVRARPAAGEVVYFLLPDRFDNGDRKNDLGGLK
CCHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCC
GDRLKTGFDPSAKGFYHGGDIKGLLSRLDYLQDMGVTALWVAPVFRNKPVQGAPGQESAG
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEHHHCCCCCCCCCCCCCCC
YHGYWVTDFTRIDPHFGSNADFKALVDAAHARGIKVYMDIIANHTADVIHYADGDYSYRN
CCCEEEEEEEEECCCCCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCCEEEECCCCCCCCC
RADWPYSRKGGLSGKAINQGFAGDEDSSAANFAKLTDPGAAYQPLIDPAERNVKVPAWLN
CCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCHHHCCEECCEEEC
DPIYYHNRGNTTFTGEDSRFGDFAGLDDLFTEHPRVRAGMVEIYADWIRRFGIDGYRVDT
CCEEEECCCCEEECCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEECC
AKHVDPGFWQVFTPEIRKVAAEAGIPNFAVFAEIANGGSDPGTIARHTRRDGFPQVLDFA
CCCCCCCCEEEECHHHHHHHHHCCCCCCEEHHHHHCCCCCCCHHHHHHCCCCCHHHHHHH
FQEAVRDLVGKGKATRVLAETFDGDVLYEGGEAAALAMPTFLGNHDMGRFSTAVRQDRPG
HHHHHHHHHCCCCHHHHHHHHCCCCEEECCCCEEEEEHHHHHCCCCCHHHHHHHHHCCCC
ISDKELLDRVALAHVMLMTLRGSPVIYYGDEQGFVGDGGDQDAREDMFPSRTASYNDNRL
CCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHHHCCCCCCCCCCCCEE
IGTASTTAADNFDEGHPLYRLIRDLAVLRKAHPALARGRQVTRTYSEGPGLFSVSRFDPE
EEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHCCCHHHHHHCCCCCEEEEECCCCC
TGTEYLIAFNTSDKPLRATSVIGASASGLEGLYGQCPLSVAAPGSVLLDLPAFGSVVCRV
CCCEEEEEECCCCCCEEHHHHHCCCCCCCCCCCCCCCCEECCCCCEEEECCHHHHHHHHH
ILSPESSVR
EECCCCCCC
>Mature Secondary Structure
MRRISLISALALVLSLGQPALADVPLAEVRARPAAGEVVYFLLPDRFDNGDRKNDLGGLK
CCHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCC
GDRLKTGFDPSAKGFYHGGDIKGLLSRLDYLQDMGVTALWVAPVFRNKPVQGAPGQESAG
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEHHHCCCCCCCCCCCCCCC
YHGYWVTDFTRIDPHFGSNADFKALVDAAHARGIKVYMDIIANHTADVIHYADGDYSYRN
CCCEEEEEEEEECCCCCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCCEEEECCCCCCCCC
RADWPYSRKGGLSGKAINQGFAGDEDSSAANFAKLTDPGAAYQPLIDPAERNVKVPAWLN
CCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCHHHCCEECCEEEC
DPIYYHNRGNTTFTGEDSRFGDFAGLDDLFTEHPRVRAGMVEIYADWIRRFGIDGYRVDT
CCEEEECCCCEEECCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEECC
AKHVDPGFWQVFTPEIRKVAAEAGIPNFAVFAEIANGGSDPGTIARHTRRDGFPQVLDFA
CCCCCCCCEEEECHHHHHHHHHCCCCCCEEHHHHHCCCCCCCHHHHHHCCCCCHHHHHHH
FQEAVRDLVGKGKATRVLAETFDGDVLYEGGEAAALAMPTFLGNHDMGRFSTAVRQDRPG
HHHHHHHHHCCCCHHHHHHHHCCCCEEECCCCEEEEEHHHHHCCCCCHHHHHHHHHCCCC
ISDKELLDRVALAHVMLMTLRGSPVIYYGDEQGFVGDGGDQDAREDMFPSRTASYNDNRL
CCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHHHCCCCCCCCCCCCEE
IGTASTTAADNFDEGHPLYRLIRDLAVLRKAHPALARGRQVTRTYSEGPGLFSVSRFDPE
EEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHCCCHHHHHHCCCCCEEEEECCCCC
TGTEYLIAFNTSDKPLRATSVIGASASGLEGLYGQCPLSVAAPGSVLLDLPAFGSVVCRV
CCCEEEEEECCCCCCEEHHHHHCCCCCCCCCCCCCCCCEECCCCCEEEECCHHHHHHHHH
ILSPESSVR
EECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8424949; 8485150 [H]