Click here to switch to the map view.

The map label for this gene is rsmB [H]

Identifier: 87199770

GI number: 87199770

Start: 1848137

End: 1849324

Strand: Direct

Name: rsmB [H]

Synonym: Saro_1753

Alternate gene names: 87199770

Gene position: 1848137-1849324 (Clockwise)

Preceding gene: 87199769

Following gene: 87199771

Centisome position: 51.89

GC content: 68.52

Gene sequence:

>1188_bases
ATGACCCCTTCCGCCCGCGTACAGGCGGCGATCGATCTGCTTGACGCGGTGATCGCGGCGGCGAAGGCGCAAGGGGCATC
TGCCGATCGGATCGCCGCGGACTGGTTCCGGTCGCGGCGCTTCGTCGGCTCGAAGGATCGCCGCGCGATCCGTGAACTCG
TGTGGAGCGCGATCCGCGTCTGCGGCGAAGTCCCGGAAAGCGGCCGCGCGGCGATGCTGCGCGTGGCCGTGGCTGACCCT
GGAATTGCCGCGCTTTTCGATGGTTCCACATATGGGCCTGCGCCGATCAGGGCAGGCGAAGTGGCGGCCGAGGGCGGCAA
CGCGCCCGCCTGGCTGAGCGAGCGCTTGCGGCAGTCCGGCCTTGGCGAGGACGAGCAGGCGGCGCTGTTGGGCAGGGCGC
CGCTCGACATCCGGGCAAACACCCTAAGGATCACGCGCGACGAACTTCGGCTGCGGCTTCCCGTCGAGTTGGAAGTCACT
GCCGCGCCCGATGCGCTGCGCCTGCCGTCCGGCACGGCCGCCGAAAGCTGGCCCGAGTTCCAGGAAGGCCTGTTCGAAGT
GCAGGATTCCGGCAGCCAGATCGCTTGCATGGCGCTGGACGTGAAGCCGGGCGAAGCCGTGGTCGATCTTTGCGCCGGGG
GTGGCGGCAAGACGCTTTCGCTCGGTGCGGCGATGGCCAACACCGGCGAACTGCTGGCGTGCGACATCGACCGCGCGCGT
CTGTCGCGGCTACCGGACCGGGCCGCGCGTGCGGGCGTACTTGCGCAGACGCGGCTGCTCAACCCCGGGAAAGAGGCCGA
GATGCTTGCCGACTGGGCGGGCAGGGCCGATGCCGTGATGGTTGACGCACCATGTTCGGGCACGGGCACCTGGCGGCGAA
ATCCCGAAGCGCGCTGGCGGCTCGATCCAAGGTCCATCGAACGCTACTGCACGATGCAGGCCAATGTTCTCGAGATCGGT
GCCGGGCTGGTGCGGCCCGGCGGACGCCTGACCTATGTGGTCTGCTCGTTGATCGATGCGGAGGGGCGCGACCGGATCGA
GGCGTTTCTTGCAGCGCATCCGGACTGGTCCGTCGCCATGCCGCCGCTTCCGGCGGGCCGTCCGCATGGCAAGGGCTGGC
GCCTTACGCCCCTTGGTGATGGTACCGACGGGTTTTTCTTCGCAACAATCGTGCGTCGTGTTAACTGA

Upstream 100 bases:

>100_bases
TTCGTGCGCATCACCAATGCGGGCCTGCGCGAAAGTCACGTCCACGACGTGACGATCACGCGCGAGGCGCCGAACTACAA
GGTGGGCTGAGAGCCACCCG

Downstream 100 bases:

>100_bases
AGGCTCATGCACGCCGGTACATTCAGCGATGGTCTCCGGCTCAAGGAGTTGCTGATGCGTTTTGCCCCCGTTGCGCTTGC
CCTTTCCCTCATCGTCGGCG

Product: Fmu (Sun)

Products: NA

Alternate protein names: 16S rRNA m5C967 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmB [H]

Number of amino acids: Translated: 395; Mature: 394

Protein sequence:

>395_residues
MTPSARVQAAIDLLDAVIAAAKAQGASADRIAADWFRSRRFVGSKDRRAIRELVWSAIRVCGEVPESGRAAMLRVAVADP
GIAALFDGSTYGPAPIRAGEVAAEGGNAPAWLSERLRQSGLGEDEQAALLGRAPLDIRANTLRITRDELRLRLPVELEVT
AAPDALRLPSGTAAESWPEFQEGLFEVQDSGSQIACMALDVKPGEAVVDLCAGGGGKTLSLGAAMANTGELLACDIDRAR
LSRLPDRAARAGVLAQTRLLNPGKEAEMLADWAGRADAVMVDAPCSGTGTWRRNPEARWRLDPRSIERYCTMQANVLEIG
AGLVRPGGRLTYVVCSLIDAEGRDRIEAFLAAHPDWSVAMPPLPAGRPHGKGWRLTPLGDGTDGFFFATIVRRVN

Sequences:

>Translated_395_residues
MTPSARVQAAIDLLDAVIAAAKAQGASADRIAADWFRSRRFVGSKDRRAIRELVWSAIRVCGEVPESGRAAMLRVAVADP
GIAALFDGSTYGPAPIRAGEVAAEGGNAPAWLSERLRQSGLGEDEQAALLGRAPLDIRANTLRITRDELRLRLPVELEVT
AAPDALRLPSGTAAESWPEFQEGLFEVQDSGSQIACMALDVKPGEAVVDLCAGGGGKTLSLGAAMANTGELLACDIDRAR
LSRLPDRAARAGVLAQTRLLNPGKEAEMLADWAGRADAVMVDAPCSGTGTWRRNPEARWRLDPRSIERYCTMQANVLEIG
AGLVRPGGRLTYVVCSLIDAEGRDRIEAFLAAHPDWSVAMPPLPAGRPHGKGWRLTPLGDGTDGFFFATIVRRVN
>Mature_394_residues
TPSARVQAAIDLLDAVIAAAKAQGASADRIAADWFRSRRFVGSKDRRAIRELVWSAIRVCGEVPESGRAAMLRVAVADPG
IAALFDGSTYGPAPIRAGEVAAEGGNAPAWLSERLRQSGLGEDEQAALLGRAPLDIRANTLRITRDELRLRLPVELEVTA
APDALRLPSGTAAESWPEFQEGLFEVQDSGSQIACMALDVKPGEAVVDLCAGGGGKTLSLGAAMANTGELLACDIDRARL
SRLPDRAARAGVLAQTRLLNPGKEAEMLADWAGRADAVMVDAPCSGTGTWRRNPEARWRLDPRSIERYCTMQANVLEIGA
GLVRPGGRLTYVVCSLIDAEGRDRIEAFLAAHPDWSVAMPPLPAGRPHGKGWRLTPLGDGTDGFFFATIVRRVN

Specific function: Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI270288816, Length=246, Percent_Identity=29.6747967479675, Blast_Score=86, Evalue=8e-17,
Organism=Homo sapiens, GI23199998, Length=246, Percent_Identity=29.6747967479675, Blast_Score=86, Evalue=9e-17,
Organism=Homo sapiens, GI8922322, Length=246, Percent_Identity=29.6747967479675, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI270288818, Length=246, Percent_Identity=29.6747967479675, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI76150625, Length=220, Percent_Identity=29.0909090909091, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI76150623, Length=220, Percent_Identity=29.0909090909091, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI32698918, Length=178, Percent_Identity=28.0898876404494, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI11545785, Length=177, Percent_Identity=32.2033898305085, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI2367212, Length=296, Percent_Identity=31.7567567567568, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI87081985, Length=246, Percent_Identity=30.0813008130081, Blast_Score=86, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17536757, Length=285, Percent_Identity=28.4210526315789, Blast_Score=88, Evalue=8e-18,
Organism=Saccharomyces cerevisiae, GI6324268, Length=288, Percent_Identity=26.3888888888889, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI22024126, Length=285, Percent_Identity=24.5614035087719, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21355201, Length=168, Percent_Identity=29.7619047619048, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI21356513, Length=238, Percent_Identity=28.5714285714286, Blast_Score=68, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24668781, Length=238, Percent_Identity=28.5714285714286, Blast_Score=68, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- InterPro:   IPR004573 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB [H]

EC number: =2.1.1.176 [H]

Molecular weight: Translated: 42118; Mature: 41987

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPSARVQAAIDLLDAVIAAAKAQGASADRIAADWFRSRRFVGSKDRRAIRELVWSAIRV
CCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
CGEVPESGRAAMLRVAVADPGIAALFDGSTYGPAPIRAGEVAAEGGNAPAWLSERLRQSG
HHCCCCCCCEEEEEEEECCCCEEEEECCCCCCCCCCCCCCEECCCCCCCHHHHHHHHHCC
LGEDEQAALLGRAPLDIRANTLRITRDELRLRLPVELEVTAAPDALRLPSGTAAESWPEF
CCCCHHHHHHCCCCCCEECCEEEEEHHHEEEECCEEEEEECCCCCEECCCCCCCCCCHHH
QEGLFEVQDSGSQIACMALDVKPGEAVVDLCAGGGGKTLSLGAAMANTGELLACDIDRAR
HHHHHHHCCCCCEEEEEEEECCCCHHHHHHHCCCCCCEEEECHHHCCCCCEEEEECCHHH
LSRLPDRAARAGVLAQTRLLNPGKEAEMLADWAGRADAVMVDAPCSGTGTWRRNPEARWR
HHCCCHHHHHHCHHHHHHCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCEEE
LDPRSIERYCTMQANVLEIGAGLVRPGGRLTYVVCSLIDAEGRDRIEAFLAAHPDWSVAM
ECHHHHHHHHHCCCCHHHHCCCCCCCCCCEEEEEEHHHCCCCHHHHHHHHHCCCCCCEEC
PPLPAGRPHGKGWRLTPLGDGTDGFFFATIVRRVN
CCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
TPSARVQAAIDLLDAVIAAAKAQGASADRIAADWFRSRRFVGSKDRRAIRELVWSAIRV
CCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
CGEVPESGRAAMLRVAVADPGIAALFDGSTYGPAPIRAGEVAAEGGNAPAWLSERLRQSG
HHCCCCCCCEEEEEEEECCCCEEEEECCCCCCCCCCCCCCEECCCCCCCHHHHHHHHHCC
LGEDEQAALLGRAPLDIRANTLRITRDELRLRLPVELEVTAAPDALRLPSGTAAESWPEF
CCCCHHHHHHCCCCCCEECCEEEEEHHHEEEECCEEEEEECCCCCEECCCCCCCCCCHHH
QEGLFEVQDSGSQIACMALDVKPGEAVVDLCAGGGGKTLSLGAAMANTGELLACDIDRAR
HHHHHHHCCCCCEEEEEEEECCCCHHHHHHHCCCCCCEEEECHHHCCCCCEEEEECCHHH
LSRLPDRAARAGVLAQTRLLNPGKEAEMLADWAGRADAVMVDAPCSGTGTWRRNPEARWR
HHCCCHHHHHHCHHHHHHCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCEEE
LDPRSIERYCTMQANVLEIGAGLVRPGGRLTYVVCSLIDAEGRDRIEAFLAAHPDWSVAM
ECHHHHHHHHHCCCCHHHHCCCCCCCCCCEEEEEEHHHCCCCHHHHHHHHHCCCCCCEEC
PPLPAGRPHGKGWRLTPLGDGTDGFFFATIVRRVN
CCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA