Click here to switch to the map view.

The map label for this gene is paaF [C]

Identifier: 87199686

GI number: 87199686

Start: 1749818

End: 1750672

Strand: Direct

Name: paaF [C]

Synonym: Saro_1669

Alternate gene names: 87199686

Gene position: 1749818-1750672 (Clockwise)

Preceding gene: 87199685

Following gene: 87199687

Centisome position: 49.13

GC content: 62.81

Gene sequence:

>855_bases
ATGACCGAACAGCAGAAGATCGAGCGCGCGGAAGAAGATACCGTCGCTTTCACCGTCGAGGACGGCATCGCTTGGGTGAA
GTTCAACCGCCCCGAGAAGCGCAACTGCATGAGCCCCAAGCTCAACCGCCGCATGAAGGCGGTGCTTGAGGAACTGGAAT
TCCGCGACGACGTGAAGGTCCTCGTGCTGACCGGTGAGGGCGATGCCTGGACCGCCGGCATGGACCTCAAGGAATACTTC
CGGGAGACCGAGGCCCAGGGCCTTGGCGCCGTGCGCAAGTCGCAGCGCGAAAGCTATGGCTGGTTCGAACGCCTGCGCTG
GTACGAGAAGCCGACCGTTGCGATGATCAACGGCTGGTGCTTCGGCGGCGGCTATGGCCCGCTGTTCGGCTGCGACATCG
CGATCGCTTCGGAAGACGCGCAGTTCGGCCTGTCGGAAATCAATTGGGGAATCCTGCCCGGCGGCGGCGCGTCGAAGGTC
GCGCAGGAACTTATGCCGTTCCGCAAGGCCATGTACCATGCGATGATGGGCGAGAACCTGACAGGCAAGCAGGCCGAAGA
GCAGGGCCTCGTCACCGAAGCCGTGCCCGCCGACAAGCTCAAGGCGCGTGTGCTGGAAATCTGCGAAGCGCTGAAGAAGA
AGGATAGCCACGCGCTTCGCGCCACCAAGTGGACGATGCGTCGCATGGTCGACATGACCTACGACAACGGCCTTGAATAC
CAGATTCGCGCGCAGGAAGCGCTGCACAGCTTCGGCGGTGCGGCAGCCCGCAAGGAAGCGACGCGCCAGTTCCTTGACGA
GAAGAGCTTCAAGCCCGGTCTCGGCACGTTCGACACGTCGAAGGTCAAGGGCTGA

Upstream 100 bases:

>100_bases
GCCGGCATCGACAGTTTCACCGAGCTGCGCTGGATCACGATGGAAACCCAGCCCGGCCACTATCCCATTTGACCTATCCG
ACTTTCTCGAAGGACAAGAC

Downstream 100 bases:

>100_bases
GAAAGACCCTGCTTGCCGTAGCGTAAAGGCCCCGGTCGCCCATGCGGCCGGGGCCTTTTGCTTGCGAAAGTGGTTGCCAG
CGAACCGCGTCCACGCCAGC

Product: p-hydroxycinnamoyl CoA hydratase/lyase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MTEQQKIERAEEDTVAFTVEDGIAWVKFNRPEKRNCMSPKLNRRMKAVLEELEFRDDVKVLVLTGEGDAWTAGMDLKEYF
RETEAQGLGAVRKSQRESYGWFERLRWYEKPTVAMINGWCFGGGYGPLFGCDIAIASEDAQFGLSEINWGILPGGGASKV
AQELMPFRKAMYHAMMGENLTGKQAEEQGLVTEAVPADKLKARVLEICEALKKKDSHALRATKWTMRRMVDMTYDNGLEY
QIRAQEALHSFGGAAARKEATRQFLDEKSFKPGLGTFDTSKVKG

Sequences:

>Translated_284_residues
MTEQQKIERAEEDTVAFTVEDGIAWVKFNRPEKRNCMSPKLNRRMKAVLEELEFRDDVKVLVLTGEGDAWTAGMDLKEYF
RETEAQGLGAVRKSQRESYGWFERLRWYEKPTVAMINGWCFGGGYGPLFGCDIAIASEDAQFGLSEINWGILPGGGASKV
AQELMPFRKAMYHAMMGENLTGKQAEEQGLVTEAVPADKLKARVLEICEALKKKDSHALRATKWTMRRMVDMTYDNGLEY
QIRAQEALHSFGGAAARKEATRQFLDEKSFKPGLGTFDTSKVKG
>Mature_283_residues
TEQQKIERAEEDTVAFTVEDGIAWVKFNRPEKRNCMSPKLNRRMKAVLEELEFRDDVKVLVLTGEGDAWTAGMDLKEYFR
ETEAQGLGAVRKSQRESYGWFERLRWYEKPTVAMINGWCFGGGYGPLFGCDIAIASEDAQFGLSEINWGILPGGGASKVA
QELMPFRKAMYHAMMGENLTGKQAEEQGLVTEAVPADKLKARVLEICEALKKKDSHALRATKWTMRRMVDMTYDNGLEYQ
IRAQEALHSFGGAAARKEATRQFLDEKSFKPGLGTFDTSKVKG

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=248, Percent_Identity=25.4032258064516, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI260274832, Length=220, Percent_Identity=31.3636363636364, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI45643119, Length=220, Percent_Identity=31.3636363636364, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI260275230, Length=220, Percent_Identity=31.3636363636364, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI31542718, Length=225, Percent_Identity=24.8888888888889, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI20127408, Length=185, Percent_Identity=27.5675675675676, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI70995211, Length=182, Percent_Identity=28.021978021978, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI68989263, Length=183, Percent_Identity=26.2295081967213, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1787659, Length=216, Percent_Identity=29.1666666666667, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI1788597, Length=216, Percent_Identity=29.6296296296296, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI221142681, Length=231, Percent_Identity=25.5411255411255, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1788682, Length=216, Percent_Identity=27.3148148148148, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1790281, Length=187, Percent_Identity=29.4117647058824, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1787660, Length=252, Percent_Identity=24.2063492063492, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17554946, Length=266, Percent_Identity=27.4436090225564, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17540714, Length=231, Percent_Identity=30.7359307359307, Blast_Score=100, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI25145438, Length=251, Percent_Identity=25.4980079681275, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17549921, Length=210, Percent_Identity=27.1428571428571, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI25144276, Length=177, Percent_Identity=28.8135593220339, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17508951, Length=177, Percent_Identity=28.8135593220339, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17508953, Length=177, Percent_Identity=28.8135593220339, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17558304, Length=177, Percent_Identity=27.1186440677966, Blast_Score=74, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17536985, Length=256, Percent_Identity=24.21875, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6320241, Length=206, Percent_Identity=23.7864077669903, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI20129971, Length=249, Percent_Identity=27.3092369477912, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24653477, Length=249, Percent_Identity=27.3092369477912, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI19922422, Length=208, Percent_Identity=26.9230769230769, Blast_Score=84, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24654903, Length=222, Percent_Identity=26.5765765765766, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI19920382, Length=239, Percent_Identity=23.8493723849372, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24653139, Length=175, Percent_Identity=27.4285714285714, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI21357171, Length=215, Percent_Identity=23.7209302325581, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 32016; Mature: 31885

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEQQKIERAEEDTVAFTVEDGIAWVKFNRPEKRNCMSPKLNRRMKAVLEELEFRDDVKV
CCCHHHHHHHCCCCEEEEEECCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEE
LVLTGEGDAWTAGMDLKEYFRETEAQGLGAVRKSQRESYGWFERLRWYEKPTVAMINGWC
EEEECCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECEE
FGGGYGPLFGCDIAIASEDAQFGLSEINWGILPGGGASKVAQELMPFRKAMYHAMMGENL
ECCCCCCHHHCEEEEECCCHHCCHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHHHCCCC
TGKQAEEQGLVTEAVPADKLKARVLEICEALKKKDSHALRATKWTMRRMVDMTYDNGLEY
CCCCHHHCCCCHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCE
QIRAQEALHSFGGAAARKEATRQFLDEKSFKPGLGTFDTSKVKG
EEEHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TEQQKIERAEEDTVAFTVEDGIAWVKFNRPEKRNCMSPKLNRRMKAVLEELEFRDDVKV
CCHHHHHHHCCCCEEEEEECCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEE
LVLTGEGDAWTAGMDLKEYFRETEAQGLGAVRKSQRESYGWFERLRWYEKPTVAMINGWC
EEEECCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECEE
FGGGYGPLFGCDIAIASEDAQFGLSEINWGILPGGGASKVAQELMPFRKAMYHAMMGENL
ECCCCCCHHHCEEEEECCCHHCCHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHHHCCCC
TGKQAEEQGLVTEAVPADKLKARVLEICEALKKKDSHALRATKWTMRRMVDMTYDNGLEY
CCCCHHHCCCCHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCE
QIRAQEALHSFGGAAARKEATRQFLDEKSFKPGLGTFDTSKVKG
EEEHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA