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The map label for this gene is trsA [H]

Identifier: 87199580

GI number: 87199580

Start: 1621111

End: 1622706

Strand: Direct

Name: trsA [H]

Synonym: Saro_1563

Alternate gene names: 87199580

Gene position: 1621111-1622706 (Clockwise)

Preceding gene: 87199579

Following gene: 87199581

Centisome position: 45.52

GC content: 65.85

Gene sequence:

>1596_bases
ATGACTGCTGGCAACTGGAACTTCGGCGACCTGCTTGATATCGCCGCCGCCAATGTCCCGACGGACCGTCCCGCGCTCGT
GCGGGGCGACCGGACGATCGCGTGGGGCGAATTCGATTGCCGCACCAACCGGCTCGCCCGCGCGATGCTGGCAAGTGGCC
TGAAGACCGGAGACCGCGTGGCCATCCTCGCGCGCAACATTCCCGAATTTATCGAGATCGCCTGCGCCGCGTTCAAGGCG
CGCCTGACCCACGTCAACATCAACTACCGCTACACCACCGCCGAAATCGAATATGTCCTTGCCGATTGCGGCGCGGCAGC
GCTGTTCCATCAGGACGAATTTGCAGGCGTCGTCGAGCCCCTCCCTGCCGCGCTCGATCATCTGAGGCTCGTCGTGCAGA
TCGGCGGGGAGGGCAGCTACGACCGGATGGTCGAAGAGGGCGACGGCACGCCGCTCGGCATCGCCCGGTCCCCCGAGGAC
GGCTATCTCCTCTATACCGGCGGCACCACCGGGCGCCCCAAGGGCGTCATGTGGGCGGCAGGCGATGCACGCGCGGTACA
GCTTGAGGCGCCAACGGTGCGCAATCCGGTGCTGACCCTGGAGGACCACGCGAAGCAGGTTGCGGCCAACATCACCCCGG
GCCGCGTGCTTCCGGCCTGTCCGCTGATGCACGGGGCGGGGCTCAACAGTTCGATGGCGGAACTCCTGATGGGCGGCACT
GCGGTGTTGCTGGAAAAGGACAGCTTTCGCGCCGACGATCTATGGGATCAGGTCGAGGGGCACCAGGTCACGCGCATCCT
GATCGTCGGCGACGTTTTCGCCCGGCCGATGCTTCAGGCGCTCGAGGCTGAACCGGGTCGCTGGGACCTGTCATGTCTCA
AGGTCATTTCCTCGGCCGGCCTGATGTGGAGCGAGGAAGTGAAGCGTGGCCTTGTCCGCCAACTTCCGCAGCTGACGCTG
GTGGATATCCTCGGTGCGTCCGAGGCCTCGGGTTTCGGCTATGCCGTGACGACCGCCACGCGTGAGACACCTACCGGCTA
TTTCGAGCCAGGTCGCCAGACCGTCATCATCGACGTCGACACCGACCGCGTGCTGGCCGACGACGAACCCGGCACAGGCT
GGCTCGCCCGCCGCCCACCTTTCGCGCAAGGCTATTTCGGCGATCCGGAAAAGACCGCGCAGACCTATCGCACCATTGGC
GGCATCACTTATGCCATCCCCGGCGACATGGCCGAACGTACTGTCGAGGGTCTCATTCGATTGATCGGTCGCGGCAATCT
GTGCATCAATACCGGCGGCGAAAAGGTCTTCGTCGAGGAAGTCGAGGAAGCACTCAAGCGAGCCCCGGGCATCGAGGACG
CCATCGTTGTCGGTGTGCCGGACGAGACTTGGGGCAAGGCCGTCGTCGCCCTGGTCCGCACTGGCGCGACCTACGACGAG
GACGCCGCACGCACGGCGCTTGCAGCAGACCTTGCGCGCTACAAGCTGCCGCGCCGGATCATCGTCCTCGACGATCTTCC
GCGCCATGCCAGCGGCAAGTCCGACTACCGCCGCGCGACCGAACTCGCCGTGGCCCACCTCGCCCTCGCGGCCTGA

Upstream 100 bases:

>100_bases
GCAAAGCTTGCCACTTCCCAAGATGCGGCAGAGGCCGTGGCTGCTTTCGTGGAGAAGCGCCCCCCGGTTTTCCTCGGTCG
CTGACGGGGAGCGCGCGACG

Downstream 100 bases:

>100_bases
CCGGTGCGGCCCCACGCCCTTGTTACCGGCGGCGCGCAGGGCATCGGCCGCGCCGTGGCCGAACTGCTTGCCGGGCAGGG
CTGGGATGTCACGGCCGCCG

Product: acyl-CoA synthetase

Products: NA

Alternate protein names: TrsI; AMP-binding ligase; Quinoxaline-2-carboxylic acid-activating enzyme [H]

Number of amino acids: Translated: 531; Mature: 530

Protein sequence:

>531_residues
MTAGNWNFGDLLDIAAANVPTDRPALVRGDRTIAWGEFDCRTNRLARAMLASGLKTGDRVAILARNIPEFIEIACAAFKA
RLTHVNINYRYTTAEIEYVLADCGAAALFHQDEFAGVVEPLPAALDHLRLVVQIGGEGSYDRMVEEGDGTPLGIARSPED
GYLLYTGGTTGRPKGVMWAAGDARAVQLEAPTVRNPVLTLEDHAKQVAANITPGRVLPACPLMHGAGLNSSMAELLMGGT
AVLLEKDSFRADDLWDQVEGHQVTRILIVGDVFARPMLQALEAEPGRWDLSCLKVISSAGLMWSEEVKRGLVRQLPQLTL
VDILGASEASGFGYAVTTATRETPTGYFEPGRQTVIIDVDTDRVLADDEPGTGWLARRPPFAQGYFGDPEKTAQTYRTIG
GITYAIPGDMAERTVEGLIRLIGRGNLCINTGGEKVFVEEVEEALKRAPGIEDAIVVGVPDETWGKAVVALVRTGATYDE
DAARTALAADLARYKLPRRIIVLDDLPRHASGKSDYRRATELAVAHLALAA

Sequences:

>Translated_531_residues
MTAGNWNFGDLLDIAAANVPTDRPALVRGDRTIAWGEFDCRTNRLARAMLASGLKTGDRVAILARNIPEFIEIACAAFKA
RLTHVNINYRYTTAEIEYVLADCGAAALFHQDEFAGVVEPLPAALDHLRLVVQIGGEGSYDRMVEEGDGTPLGIARSPED
GYLLYTGGTTGRPKGVMWAAGDARAVQLEAPTVRNPVLTLEDHAKQVAANITPGRVLPACPLMHGAGLNSSMAELLMGGT
AVLLEKDSFRADDLWDQVEGHQVTRILIVGDVFARPMLQALEAEPGRWDLSCLKVISSAGLMWSEEVKRGLVRQLPQLTL
VDILGASEASGFGYAVTTATRETPTGYFEPGRQTVIIDVDTDRVLADDEPGTGWLARRPPFAQGYFGDPEKTAQTYRTIG
GITYAIPGDMAERTVEGLIRLIGRGNLCINTGGEKVFVEEVEEALKRAPGIEDAIVVGVPDETWGKAVVALVRTGATYDE
DAARTALAADLARYKLPRRIIVLDDLPRHASGKSDYRRATELAVAHLALAA
>Mature_530_residues
TAGNWNFGDLLDIAAANVPTDRPALVRGDRTIAWGEFDCRTNRLARAMLASGLKTGDRVAILARNIPEFIEIACAAFKAR
LTHVNINYRYTTAEIEYVLADCGAAALFHQDEFAGVVEPLPAALDHLRLVVQIGGEGSYDRMVEEGDGTPLGIARSPEDG
YLLYTGGTTGRPKGVMWAAGDARAVQLEAPTVRNPVLTLEDHAKQVAANITPGRVLPACPLMHGAGLNSSMAELLMGGTA
VLLEKDSFRADDLWDQVEGHQVTRILIVGDVFARPMLQALEAEPGRWDLSCLKVISSAGLMWSEEVKRGLVRQLPQLTLV
DILGASEASGFGYAVTTATRETPTGYFEPGRQTVIIDVDTDRVLADDEPGTGWLARRPPFAQGYFGDPEKTAQTYRTIGG
ITYAIPGDMAERTVEGLIRLIGRGNLCINTGGEKVFVEEVEEALKRAPGIEDAIVVGVPDETWGKAVVALVRTGATYDED
AARTALAADLARYKLPRRIIVLDDLPRHASGKSDYRRATELAVAHLALAA

Specific function: Involved in triostin biosynthesis. Activates quinoxaline-2-carboxylic acid (QA) via catalysis of the ATP- pyrophosphate exchange reaction dependent on QA, and the formation of the corresponding adenylate. Also activates structural analogs of QA such as qu

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=560, Percent_Identity=22.6785714285714, Blast_Score=108, Evalue=2e-23,
Organism=Homo sapiens, GI187761345, Length=549, Percent_Identity=26.5938069216758, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI187761343, Length=549, Percent_Identity=26.5938069216758, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI28416953, Length=520, Percent_Identity=25.3846153846154, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI38505220, Length=489, Percent_Identity=23.1083844580777, Blast_Score=79, Evalue=8e-15,
Organism=Escherichia coli, GI145693145, Length=535, Percent_Identity=28.785046728972, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1788107, Length=547, Percent_Identity=24.8628884826325, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI1786810, Length=539, Percent_Identity=25.417439703154, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI221142682, Length=503, Percent_Identity=25.6461232604374, Blast_Score=92, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17557194, Length=511, Percent_Identity=24.4618395303327, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17560308, Length=566, Percent_Identity=23.321554770318, Blast_Score=99, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17538037, Length=507, Percent_Identity=24.0631163708087, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI32563687, Length=519, Percent_Identity=24.0847784200385, Blast_Score=90, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17531443, Length=514, Percent_Identity=21.4007782101167, Blast_Score=83, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17558820, Length=544, Percent_Identity=21.5073529411765, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17559526, Length=366, Percent_Identity=23.4972677595628, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6319699, Length=509, Percent_Identity=24.5579567779961, Blast_Score=91, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6323182, Length=556, Percent_Identity=23.021582733813, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6319264, Length=565, Percent_Identity=24.2477876106195, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24581924, Length=555, Percent_Identity=23.7837837837838, Blast_Score=81, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: NA

Molecular weight: Translated: 57257; Mature: 57126

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAGNWNFGDLLDIAAANVPTDRPALVRGDRTIAWGEFDCRTNRLARAMLASGLKTGDRV
CCCCCCCCHHHHHHHHCCCCCCCCCEEECCCEEEECCCCHHHHHHHHHHHHHCCCCCCEE
AILARNIPEFIEIACAAFKARLTHVNINYRYTTAEIEYVLADCGAAALFHQDEFAGVVEP
EEEECCCHHHHHHHHHHHHHEEEEEEEEEEEEHHEEEEEECCCCHHHEECCHHHHHHHHH
LPAALDHLRLVVQIGGEGSYDRMVEEGDGTPLGIARSPEDGYLLYTGGTTGRPKGVMWAA
HHHHHHHEEEEEEECCCCCHHHHHHCCCCCEECEEECCCCCEEEEECCCCCCCCCEEEEC
GDARAVQLEAPTVRNPVLTLEDHAKQVAANITPGRVLPACPLMHGAGLNSSMAELLMGGT
CCCEEEEEECCCCCCCEEEHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCE
AVLLEKDSFRADDLWDQVEGHQVTRILIVGDVFARPMLQALEAEPGRWDLSCLKVISSAG
EEEEECCCCCCHHHHHHCCCCEEEEEEEECHHHHHHHHHHHCCCCCCCHHHHHHHHHHCC
LMWSEEVKRGLVRQLPQLTLVDILGASEASGFGYAVTTATRETPTGYFEPGRQTVIIDVD
CEEHHHHHHHHHHHCCCEEEHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCEEEEEEEC
TDRVLADDEPGTGWLARRPPFAQGYFGDPEKTAQTYRTIGGITYAIPGDMAERTVEGLIR
CCCEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHH
LIGRGNLCINTGGEKVFVEEVEEALKRAPGIEDAIVVGVPDETWGKAVVALVRTGATYDE
HHCCCCEEEECCCCEEEHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCH
DAARTALAADLARYKLPRRIIVLDDLPRHASGKSDYRRATELAVAHLALAA
HHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TAGNWNFGDLLDIAAANVPTDRPALVRGDRTIAWGEFDCRTNRLARAMLASGLKTGDRV
CCCCCCCHHHHHHHHCCCCCCCCCEEECCCEEEECCCCHHHHHHHHHHHHHCCCCCCEE
AILARNIPEFIEIACAAFKARLTHVNINYRYTTAEIEYVLADCGAAALFHQDEFAGVVEP
EEEECCCHHHHHHHHHHHHHEEEEEEEEEEEEHHEEEEEECCCCHHHEECCHHHHHHHHH
LPAALDHLRLVVQIGGEGSYDRMVEEGDGTPLGIARSPEDGYLLYTGGTTGRPKGVMWAA
HHHHHHHEEEEEEECCCCCHHHHHHCCCCCEECEEECCCCCEEEEECCCCCCCCCEEEEC
GDARAVQLEAPTVRNPVLTLEDHAKQVAANITPGRVLPACPLMHGAGLNSSMAELLMGGT
CCCEEEEEECCCCCCCEEEHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCE
AVLLEKDSFRADDLWDQVEGHQVTRILIVGDVFARPMLQALEAEPGRWDLSCLKVISSAG
EEEEECCCCCCHHHHHHCCCCEEEEEEEECHHHHHHHHHHHCCCCCCCHHHHHHHHHHCC
LMWSEEVKRGLVRQLPQLTLVDILGASEASGFGYAVTTATRETPTGYFEPGRQTVIIDVD
CEEHHHHHHHHHHHCCCEEEHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCEEEEEEEC
TDRVLADDEPGTGWLARRPPFAQGYFGDPEKTAQTYRTIGGITYAIPGDMAERTVEGLIR
CCCEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHH
LIGRGNLCINTGGEKVFVEEVEEALKRAPGIEDAIVVGVPDETWGKAVVALVRTGATYDE
HHCCCCEEEECCCCEEEHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCH
DAARTALAADLARYKLPRRIIVLDDLPRHASGKSDYRRATELAVAHLALAA
HHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA