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The map label for this gene is trmD

Identifier: 87199428

GI number: 87199428

Start: 1450001

End: 1450741

Strand: Direct

Name: trmD

Synonym: Saro_1407

Alternate gene names: 87199428

Gene position: 1450001-1450741 (Clockwise)

Preceding gene: 87199427

Following gene: 87199429

Centisome position: 40.71

GC content: 65.72

Gene sequence:

>741_bases
ATGACCTTCGCCGCCACCGTACTTACTCTCTATCCCGAGATGTTTCCCGGTCCGCTGGGCGTGAGCCTTGCCGGGCGCGC
GCTGCGCGAGGGGGCGTGGGCGATGGATGCGGTGCAGATCCGCGATTTCGCCATCGACAAGCACAAGACCGTCGACGACA
CGCCCGCTGGTGGCGGGGCGGGGATGGTGTTGCGGGTGGATGTGCTGGCCAAGGCCATCGACCATGCTCGAGAGGCGCAT
CCGGGGTGCCCGGTTATCGCCATGACGCCGCGCGGCAAGCCGCTCACGCAGGAGCGTGTGCGGCAGCTTGCGGACGGGCC
GGGCGTGATCGTCCTGTGTGGCCGGTTCGAGGGCTTTGATGAGCGGATTTTCGCCGGGAGAGAGGTCGAGGAAGTCTCGG
TCGGCGACATCGTGCTGTCCGGCGGCGAATGTGCCGCCCTGATGCTGCTGGACGCTTGCATTCGGCTGCTTCCCGGCGTA
ATGGGCGCGGCTTCTAGCGGGCATGAGGAGTCGTTCGAGAACGGCCTCCTGGAATACCCGCACTATACCCGACCTGCTGA
GTGGGAAGGGCGCACGATCCCTGAAGTGCTGCGATCGGGGGATCATGCGAAGATCGCGGCTTGGCGCAAGTCACAGTCCG
AGATCGATACACGGTTACGCAGGCCGGACCTTTGGGAGCGCCACATCGGCGCTCGGGTCCAGTCTGCCTCTGGCGCGCAG
CGTGAAGTGCAGGACGATTGA

Upstream 100 bases:

>100_bases
GCAAGGCCGACGGCAAGAAGTTCATGGTGCCGATGAAGACGGAAGCGGTTCCGGAATGGACCGACGAGCGGATTGTCATT
GAAGCGGCTTACGCTGACCA

Downstream 100 bases:

>100_bases
AAAATGAACCTGATTCAGCAGCTTGAAGCTGAAGCTATCGCCGAATTCAAGGCGAAGAAGGAAATTCCGGACTTCCGCGC
CGGCGATACCGTTCGCGTTG

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 246; Mature: 245

Protein sequence:

>246_residues
MTFAATVLTLYPEMFPGPLGVSLAGRALREGAWAMDAVQIRDFAIDKHKTVDDTPAGGGAGMVLRVDVLAKAIDHAREAH
PGCPVIAMTPRGKPLTQERVRQLADGPGVIVLCGRFEGFDERIFAGREVEEVSVGDIVLSGGECAALMLLDACIRLLPGV
MGAASSGHEESFENGLLEYPHYTRPAEWEGRTIPEVLRSGDHAKIAAWRKSQSEIDTRLRRPDLWERHIGARVQSASGAQ
REVQDD

Sequences:

>Translated_246_residues
MTFAATVLTLYPEMFPGPLGVSLAGRALREGAWAMDAVQIRDFAIDKHKTVDDTPAGGGAGMVLRVDVLAKAIDHAREAH
PGCPVIAMTPRGKPLTQERVRQLADGPGVIVLCGRFEGFDERIFAGREVEEVSVGDIVLSGGECAALMLLDACIRLLPGV
MGAASSGHEESFENGLLEYPHYTRPAEWEGRTIPEVLRSGDHAKIAAWRKSQSEIDTRLRRPDLWERHIGARVQSASGAQ
REVQDD
>Mature_245_residues
TFAATVLTLYPEMFPGPLGVSLAGRALREGAWAMDAVQIRDFAIDKHKTVDDTPAGGGAGMVLRVDVLAKAIDHAREAHP
GCPVIAMTPRGKPLTQERVRQLADGPGVIVLCGRFEGFDERIFAGREVEEVSVGDIVLSGGECAALMLLDACIRLLPGVM
GAASSGHEESFENGLLEYPHYTRPAEWEGRTIPEVLRSGDHAKIAAWRKSQSEIDTRLRRPDLWERHIGARVQSASGAQR
EVQDD

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=222, Percent_Identity=44.5945945945946, Blast_Score=182, Evalue=2e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_NOVAD (Q2G8H2)

Other databases:

- EMBL:   CP000248
- RefSeq:   YP_496685.1
- ProteinModelPortal:   Q2G8H2
- SMR:   Q2G8H2
- STRING:   Q2G8H2
- GeneID:   3916071
- GenomeReviews:   CP000248_GR
- KEGG:   nar:Saro_1407
- NMPDR:   fig|48935.1.peg.2261
- eggNOG:   COG0336
- HOGENOM:   HBG285805
- OMA:   VCGRFEG
- ProtClustDB:   PRK00026
- BioCyc:   NARO279238:SARO_1407-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 26705; Mature: 26574

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: NA

Important sites: BINDING 114-114

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFAATVLTLYPEMFPGPLGVSLAGRALREGAWAMDAVQIRDFAIDKHKTVDDTPAGGGA
CCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCC
GMVLRVDVLAKAIDHAREAHPGCPVIAMTPRGKPLTQERVRQLADGPGVIVLCGRFEGFD
CCEEHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHCCCCEEEEECCCCCCH
ERIFAGREVEEVSVGDIVLSGGECAALMLLDACIRLLPGVMGAASSGHEESFENGLLEYP
HHHHCCCCCCEEEECEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCC
HYTRPAEWEGRTIPEVLRSGDHAKIAAWRKSQSEIDTRLRRPDLWERHIGARVQSASGAQ
CCCCCCCCCCCCHHHHHHCCCCCEEHHHHHHHHHHHHHHCCCCHHHHHHCCHHHCCCCCC
REVQDD
CCCCCC
>Mature Secondary Structure 
TFAATVLTLYPEMFPGPLGVSLAGRALREGAWAMDAVQIRDFAIDKHKTVDDTPAGGGA
CHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCC
GMVLRVDVLAKAIDHAREAHPGCPVIAMTPRGKPLTQERVRQLADGPGVIVLCGRFEGFD
CCEEHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHCCCCEEEEECCCCCCH
ERIFAGREVEEVSVGDIVLSGGECAALMLLDACIRLLPGVMGAASSGHEESFENGLLEYP
HHHHCCCCCCEEEECEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCC
HYTRPAEWEGRTIPEVLRSGDHAKIAAWRKSQSEIDTRLRRPDLWERHIGARVQSASGAQ
CCCCCCCCCCCCHHHHHHCCCCCEEHHHHHHHHHHHHHHCCCCHHHHHHCCHHHCCCCCC
REVQDD
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA