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The map label for this gene is pcbC [H]

Identifier: 87199351

GI number: 87199351

Start: 1372662

End: 1373597

Strand: Reverse

Name: pcbC [H]

Synonym: Saro_1330

Alternate gene names: 87199351

Gene position: 1373597-1372662 (Counterclockwise)

Preceding gene: 87199355

Following gene: 87199347

Centisome position: 38.57

GC content: 66.03

Gene sequence:

>936_bases
ATGCCTCTCGAAACCCTGCCGGTGATCTCGCTCGCCGGCGAACCTGATGCGCTTTCCCGCGAACTGGGAGAATCCTTCAG
GACCTTCGGCTTCGCCATGGTCCGCGACCACGGCATCGACCCCGACCTCATCGCCCGCGCCTGGGACCTGACCGCCCAAT
TCTTCGCCCTGCCCGAAGCCGAGAAGCGCAGCTATTACCTTGATGGCCTTGCCGGCGCGCGGGGCTACACGCCGTTCGGC
ACGGAGATCGCCAAGGGAGCAAGGCTCCACGATCTCAAGGAGTTCTGGCACGTAGGGCGCGACCTGCCGGCCGGACATCC
GCTGTCTGCCTCGATGCCGCCCAACGTGTGGCCCGCGCGCCCCGAAGGCTTCCGCCAGACGTTCGAGACGCTCTACGGCG
AATTCGACAAGGTCGGCGCACGCATCCTCTCGCGCATCGCGGTCTGGCTCGGGCTGGACGAGAACTGGTTCGACCCGGCG
ATCGAGGACGGCAACTCGGTCATGCGCCTGCTCCACTACCCTCCCGTCCCCGATGCCGAATCCGGCGCCATCCGCGCCGG
CGCGCACGAGGACATCAACCTCATCACACTTCTCCTCGGCGCCGAGGAAGCGGGTCTGGAACTGCTCAGCAGGCAGGGCG
AATGGATTGGCGTTTCCCCGCCCGAGGGCGCGCTGGTGGTCAACATCGGCGACATGCTCCAGCGGCTCACCAACCACGTC
CTTCCATCCACCACGCACCGCGTGCGCAATCCGGAAGGGGAGCGGGCGCGGTTCAGCCGCTACTCGATGCCGTTCTTCCT
GCACCTGAGAAGCGATTTCCCGTTCGTGACGCTGCCGCAGTGCATCTCGGACGAGAATCCCGACCGCTACCCGGTCTCGA
TCACCGCTGACGATTACCTGCAGGAACGCCTGCGCGAGATCGGCCTGCGCAAGTAA

Upstream 100 bases:

>100_bases
GCGGCCGGCCGTGTCGGCGATGTTGATTTTCGTCATGAAACCCCGTTCGAATCCCGTTACAGGCGGTTTCCATAGCGACA
AATTTGCGAACTGGAACACC

Downstream 100 bases:

>100_bases
CGCCCGCGCCGTACCGGGGCCCGCGTCGCGGACACCCGAGTGCGGCCAGGTATCGTTAGGTGCTTTTAGGTGCCGTTCGG
GTCATTCGGCCTTTTCGAGC

Product: 2OG-Fe(II) oxygenase

Products: NA

Alternate protein names: IPNS [H]

Number of amino acids: Translated: 311; Mature: 310

Protein sequence:

>311_residues
MPLETLPVISLAGEPDALSRELGESFRTFGFAMVRDHGIDPDLIARAWDLTAQFFALPEAEKRSYYLDGLAGARGYTPFG
TEIAKGARLHDLKEFWHVGRDLPAGHPLSASMPPNVWPARPEGFRQTFETLYGEFDKVGARILSRIAVWLGLDENWFDPA
IEDGNSVMRLLHYPPVPDAESGAIRAGAHEDINLITLLLGAEEAGLELLSRQGEWIGVSPPEGALVVNIGDMLQRLTNHV
LPSTTHRVRNPEGERARFSRYSMPFFLHLRSDFPFVTLPQCISDENPDRYPVSITADDYLQERLREIGLRK

Sequences:

>Translated_311_residues
MPLETLPVISLAGEPDALSRELGESFRTFGFAMVRDHGIDPDLIARAWDLTAQFFALPEAEKRSYYLDGLAGARGYTPFG
TEIAKGARLHDLKEFWHVGRDLPAGHPLSASMPPNVWPARPEGFRQTFETLYGEFDKVGARILSRIAVWLGLDENWFDPA
IEDGNSVMRLLHYPPVPDAESGAIRAGAHEDINLITLLLGAEEAGLELLSRQGEWIGVSPPEGALVVNIGDMLQRLTNHV
LPSTTHRVRNPEGERARFSRYSMPFFLHLRSDFPFVTLPQCISDENPDRYPVSITADDYLQERLREIGLRK
>Mature_310_residues
PLETLPVISLAGEPDALSRELGESFRTFGFAMVRDHGIDPDLIARAWDLTAQFFALPEAEKRSYYLDGLAGARGYTPFGT
EIAKGARLHDLKEFWHVGRDLPAGHPLSASMPPNVWPARPEGFRQTFETLYGEFDKVGARILSRIAVWLGLDENWFDPAI
EDGNSVMRLLHYPPVPDAESGAIRAGAHEDINLITLLLGAEEAGLELLSRQGEWIGVSPPEGALVVNIGDMLQRLTNHVL
PSTTHRVRNPEGERARFSRYSMPFFLHLRSDFPFVTLPQCISDENPDRYPVSITADDYLQERLREIGLRK

Specific function: Removes, in the presence of oxygen, 4 hydrogen atoms from delta-L-(alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV) to form the azetidinone and thiazolidine rings of isopenicillin [H]

COG id: COG3491

COG function: function code R; Isopenicillin N synthase and related dioxygenases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe2OG dioxygenase domain [H]

Homologues:

Organism=Drosophila melanogaster, GI85816264, Length=278, Percent_Identity=28.7769784172662, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI28572124, Length=286, Percent_Identity=29.3706293706294, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002057
- InterPro:   IPR002283
- InterPro:   IPR005123 [H]

Pfam domain/function: PF03171 2OG-FeII_Oxy [H]

EC number: =1.21.3.1 [H]

Molecular weight: Translated: 34830; Mature: 34699

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLETLPVISLAGEPDALSRELGESFRTFGFAMVRDHGIDPDLIARAWDLTAQFFALPEA
CCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCH
EKRSYYLDGLAGARGYTPFGTEIAKGARLHDLKEFWHVGRDLPAGHPLSASMPPNVWPAR
HHCCEEECCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
PEGFRQTFETLYGEFDKVGARILSRIAVWLGLDENWFDPAIEDGNSVMRLLHYPPVPDAE
CHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCHHHHHHHCCCCCCCCC
SGAIRAGAHEDINLITLLLGAEEAGLELLSRQGEWIGVSPPEGALVVNIGDMLQRLTNHV
CCCEECCCCCCCEEEEEEECCCHHHHHHHHCCCCEEECCCCCCEEEEEHHHHHHHHHHHC
LPSTTHRVRNPEGERARFSRYSMPFFLHLRSDFPFVTLPQCISDENPDRYPVSITADDYL
CCCCHHHHCCCCCCHHHHHHCCCCEEEEECCCCCEEECHHHHCCCCCCCCEEEECHHHHH
QERLREIGLRK
HHHHHHCCCCC
>Mature Secondary Structure 
PLETLPVISLAGEPDALSRELGESFRTFGFAMVRDHGIDPDLIARAWDLTAQFFALPEA
CCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCH
EKRSYYLDGLAGARGYTPFGTEIAKGARLHDLKEFWHVGRDLPAGHPLSASMPPNVWPAR
HHCCEEECCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
PEGFRQTFETLYGEFDKVGARILSRIAVWLGLDENWFDPAIEDGNSVMRLLHYPPVPDAE
CHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCHHHHHHHCCCCCCCCC
SGAIRAGAHEDINLITLLLGAEEAGLELLSRQGEWIGVSPPEGALVVNIGDMLQRLTNHV
CCCEECCCCCCCEEEEEEECCCHHHHHHHHCCCCEEECCCCCCEEEEEHHHHHHHHHHHC
LPSTTHRVRNPEGERARFSRYSMPFFLHLRSDFPFVTLPQCISDENPDRYPVSITADDYL
CCCCHHHHCCCCCCHHHHHHCCCCEEEEECCCCCEEECHHHHCCCCCCCCEEEECHHHHH
QERLREIGLRK
HHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3130293 [H]