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The map label for this gene is folC [H]

Identifier: 87199320

GI number: 87199320

Start: 1344074

End: 1345381

Strand: Reverse

Name: folC [H]

Synonym: Saro_1299

Alternate gene names: 87199320

Gene position: 1345381-1344074 (Counterclockwise)

Preceding gene: 87199321

Following gene: 87199318

Centisome position: 37.77

GC content: 68.96

Gene sequence:

>1308_bases
TTGAAGGACTTCGCAATCTCCGAAAACCCGGCGGTCCAGGCGCAGCTTGACCGCCTCGGCGCGCTGTCGCTCCCATCGGG
GCGGCTTGGCCTAGATACCGCGCGCGAACTCTGCGCCCGGCTCGGCAATCCGCAGGACCGCCTGCCGCCGGTGTTCCACG
TTGCAGGGACGAACGGCAAGGGCTCCACCTGCACTTACCTGCGCTACATCCTCGAGGCCGAGGGCCTGACGGTCCACTCC
GCCACGAAGCCGCACCTCGTCCGCTACAACGAACGCATCCGTCTCGCCGGCAAGCTGATCGAGGACGAACTGCTGGCGGA
CCTGCTCAAGGAAGTGCTCGATGCGGGCGCGGACCTCGAGCCGAGCTTCTTCGAGGTGACCACCGTCGCCACCTTCCTCG
CCTATGCCCGCACGCCGGCGGACGCCTGCGTGATCGAGACCGGACTTGGCGGCAGGCTCGACGCAACCAACGTGATGCCC
ACGGCGGCTGTGTGCGGCATCGCCACGCTGGGGCTGGACCACGAAGCCTTCCTGCTCTCGCCCGAAGATGGTGTGCCCAC
CGAACCGCTGGCGAGAATCGCCTTCGAAAAGGCCAGCATTGCCAAGCCCGGCGTGCCGCTGGTCACAATGGCCTACCCCC
CGCTTGCCGCCGATGAGGTCGCGCGCACCGCCGCCCGGCTCGGCGCACCGCTCATCATGCGCGGACGCGACTGGGACGCG
GAAATCGCCGGCTCGATCCTTTACCGCGACGAACGCGGCGAACTGCCGCTGCCCCTGCCCGCCATGCCCGGCGTCCATCA
GGCGGAAAACGCCGCGCTGGCTGTCGCCATGATCCGCCATCAGAACGCGGTGCAGGTCCATCTCGGCGCGGTCGGCAAGG
GCATCGTCGATGCGCGCTGGCCCGCCCGCCTGCAACGGCTGGGCCATGGCCCGCTCGGCGCGTTGGCCGGCAATCGCACG
ATCTGGCTCGATGGCGGCCACAATCCCGATGCGGGCCGGGCCATCGCGCGGCATCTGGAAGGGCAGCCGCCGGTCCACTT
GATCATGGGCATGCTGGCAAACAAGGACCCGTCTGCGATCCTCAGGCCGCTGGCGGACCGGGCCCTGTCGATCTCGGTCG
TCCCCGCGCCGGGGCACGACGCGCACAAGCCGGAGGACTTCGCGCCGTTCACGAGCCTTGCAGTGGGCAGCTTCGCCACC
GTGCCCGAGGCGCTCGCGGCGCTCCCGCCTGAAGGCGATGTCCTGATCGTCGGCTCACTCTACCTCGCAGGCGAAGTGCT
GCGCCTGAACGAAGAGATTCCCGACTGA

Upstream 100 bases:

>100_bases
CGCCGCGACCTCAAGACCACCCTGGCGCAGGTCATCGACTTCCTCATGGCGGGCAAGGCGGCGGCGTGAGCGCCATCCTC
CCCGCTCCGGAGAGGATGGT

Downstream 100 bases:

>100_bases
CCTGCCGTTCGTGTCGAGCGAAGGTTGGAGTTCAGGTAAGCGGATCGGCGTCCGACTTCTCTTCGCCGGCCGTGCCCATC
GCCGCATCGACCAGGCCGCT

Product: FolC bifunctional protein

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MKDFAISENPAVQAQLDRLGALSLPSGRLGLDTARELCARLGNPQDRLPPVFHVAGTNGKGSTCTYLRYILEAEGLTVHS
ATKPHLVRYNERIRLAGKLIEDELLADLLKEVLDAGADLEPSFFEVTTVATFLAYARTPADACVIETGLGGRLDATNVMP
TAAVCGIATLGLDHEAFLLSPEDGVPTEPLARIAFEKASIAKPGVPLVTMAYPPLAADEVARTAARLGAPLIMRGRDWDA
EIAGSILYRDERGELPLPLPAMPGVHQAENAALAVAMIRHQNAVQVHLGAVGKGIVDARWPARLQRLGHGPLGALAGNRT
IWLDGGHNPDAGRAIARHLEGQPPVHLIMGMLANKDPSAILRPLADRALSISVVPAPGHDAHKPEDFAPFTSLAVGSFAT
VPEALAALPPEGDVLIVGSLYLAGEVLRLNEEIPD

Sequences:

>Translated_435_residues
MKDFAISENPAVQAQLDRLGALSLPSGRLGLDTARELCARLGNPQDRLPPVFHVAGTNGKGSTCTYLRYILEAEGLTVHS
ATKPHLVRYNERIRLAGKLIEDELLADLLKEVLDAGADLEPSFFEVTTVATFLAYARTPADACVIETGLGGRLDATNVMP
TAAVCGIATLGLDHEAFLLSPEDGVPTEPLARIAFEKASIAKPGVPLVTMAYPPLAADEVARTAARLGAPLIMRGRDWDA
EIAGSILYRDERGELPLPLPAMPGVHQAENAALAVAMIRHQNAVQVHLGAVGKGIVDARWPARLQRLGHGPLGALAGNRT
IWLDGGHNPDAGRAIARHLEGQPPVHLIMGMLANKDPSAILRPLADRALSISVVPAPGHDAHKPEDFAPFTSLAVGSFAT
VPEALAALPPEGDVLIVGSLYLAGEVLRLNEEIPD
>Mature_435_residues
MKDFAISENPAVQAQLDRLGALSLPSGRLGLDTARELCARLGNPQDRLPPVFHVAGTNGKGSTCTYLRYILEAEGLTVHS
ATKPHLVRYNERIRLAGKLIEDELLADLLKEVLDAGADLEPSFFEVTTVATFLAYARTPADACVIETGLGGRLDATNVMP
TAAVCGIATLGLDHEAFLLSPEDGVPTEPLARIAFEKASIAKPGVPLVTMAYPPLAADEVARTAARLGAPLIMRGRDWDA
EIAGSILYRDERGELPLPLPAMPGVHQAENAALAVAMIRHQNAVQVHLGAVGKGIVDARWPARLQRLGHGPLGALAGNRT
IWLDGGHNPDAGRAIARHLEGQPPVHLIMGMLANKDPSAILRPLADRALSISVVPAPGHDAHKPEDFAPFTSLAVGSFAT
VPEALAALPPEGDVLIVGSLYLAGEVLRLNEEIPD

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932990, Length=364, Percent_Identity=30.7692307692308, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI66932984, Length=364, Percent_Identity=30.7692307692308, Blast_Score=137, Evalue=2e-32,
Organism=Escherichia coli, GI1788654, Length=411, Percent_Identity=30.9002433090024, Blast_Score=139, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI71984923, Length=350, Percent_Identity=27.7142857142857, Blast_Score=116, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17553148, Length=350, Percent_Identity=27.7142857142857, Blast_Score=116, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17553150, Length=350, Percent_Identity=27.7142857142857, Blast_Score=115, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6323760, Length=433, Percent_Identity=31.4087759815243, Blast_Score=172, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6322718, Length=444, Percent_Identity=24.5495495495495, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6324815, Length=319, Percent_Identity=27.2727272727273, Blast_Score=97, Evalue=7e-21,
Organism=Drosophila melanogaster, GI24641571, Length=185, Percent_Identity=30.8108108108108, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24581568, Length=184, Percent_Identity=32.0652173913043, Blast_Score=97, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 46034; Mature: 46034

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1 ; PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDFAISENPAVQAQLDRLGALSLPSGRLGLDTARELCARLGNPQDRLPPVFHVAGTNGK
CCCCCCCCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCHHHCCCEEEEECCCCC
GSTCTYLRYILEAEGLTVHSATKPHLVRYNERIRLAGKLIEDELLADLLKEVLDAGADLE
CCHHHHHHHHHHHCCEEEECCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PSFFEVTTVATFLAYARTPADACVIETGLGGRLDATNVMPTAAVCGIATLGLDHEAFLLS
CCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEC
PEDGVPTEPLARIAFEKASIAKPGVPLVTMAYPPLAADEVARTAARLGAPLIMRGRDWDA
CCCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEECCCCCH
EIAGSILYRDERGELPLPLPAMPGVHQAENAALAVAMIRHQNAVQVHLGAVGKGIVDARW
HHHCEEEEECCCCCCCCCCCCCCCCCCCCCHHEEEHHHHCCCEEEEEECCCCCCCCCCCC
PARLQRLGHGPLGALAGNRTIWLDGGHNPDAGRAIARHLEGQPPVHLIMGMLANKDPSAI
HHHHHHCCCCCCHHCCCCCEEEECCCCCCCHHHHHHHHCCCCCHHHHHHHHHCCCCHHHH
LRPLADRALSISVVPAPGHDAHKPEDFAPFTSLAVGSFATVPEALAALPPEGDVLIVGSL
HHHHHCCCEEEEEEECCCCCCCCCCCCCCHHHHHHCCHHHHHHHHHCCCCCCCEEEEECH
YLAGEVLRLNEEIPD
HHHHHHHHHCCCCCC
>Mature Secondary Structure
MKDFAISENPAVQAQLDRLGALSLPSGRLGLDTARELCARLGNPQDRLPPVFHVAGTNGK
CCCCCCCCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCHHHCCCEEEEECCCCC
GSTCTYLRYILEAEGLTVHSATKPHLVRYNERIRLAGKLIEDELLADLLKEVLDAGADLE
CCHHHHHHHHHHHCCEEEECCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PSFFEVTTVATFLAYARTPADACVIETGLGGRLDATNVMPTAAVCGIATLGLDHEAFLLS
CCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEC
PEDGVPTEPLARIAFEKASIAKPGVPLVTMAYPPLAADEVARTAARLGAPLIMRGRDWDA
CCCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEECCCCCH
EIAGSILYRDERGELPLPLPAMPGVHQAENAALAVAMIRHQNAVQVHLGAVGKGIVDARW
HHHCEEEEECCCCCCCCCCCCCCCCCCCCCHHEEEHHHHCCCEEEEEECCCCCCCCCCCC
PARLQRLGHGPLGALAGNRTIWLDGGHNPDAGRAIARHLEGQPPVHLIMGMLANKDPSAI
HHHHHHCCCCCCHHCCCCCEEEECCCCCCCHHHHHHHHCCCCCHHHHHHHHHCCCCHHHH
LRPLADRALSISVVPAPGHDAHKPEDFAPFTSLAVGSFATVPEALAALPPEGDVLIVGSL
HHHHHCCCEEEEEEECCCCCCCCCCCCCCHHHHHHCCHHHHHHHHHCCCCCCCEEEEECH
YLAGEVLRLNEEIPD
HHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]