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The map label for this gene is btuB [H]

Identifier: 87199208

GI number: 87199208

Start: 1228490

End: 1230394

Strand: Direct

Name: btuB [H]

Synonym: Saro_1186

Alternate gene names: 87199208

Gene position: 1228490-1230394 (Clockwise)

Preceding gene: 87199196

Following gene: 87199209

Centisome position: 34.49

GC content: 69.03

Gene sequence:

>1905_bases
ATGAAGAAGTACCTGCTTTCCGTTTCCGCCGTCGCGTTCGCGTGCCCGGCGCTTGCGCAGGCCGCCAACGATGTCGTCGT
GGCCGACCGCCGTGCCGATCCCGCCGCAATTACCGTTATCGCTACCGGTAGCGAGACCCTGGTCAGCCGAGTCGGCCAGC
CGGTCACCGTCATCGCCGCCGACGAGATCCGGTCGATTCAGGGCCCTGACATCGCCCGCGTCCTCGAGCGCGTCCCCGGC
CTCGCGCTCACCCGCAATGGCGGTCCCGGAAGCTTTACCGGCGTGCGCCTGCGCGGCTCTGATGCCGAACAGGTACTTGT
CCTCGTCGACGGCGTCCGCGTCGAGGATGTCTCGGCCCCCTCCGGCGGCTTCGATTTTGGCACGCTCACCCCCGGCGGGA
TCGAGCGCATCGACGTGCTGCGCGGCTCCAACTCGATCGTCTGGGGCAGCGCCGCGATCGGCGGCGTGATCGCCGTCCAG
TCGCGCGAACTCGATGGCATCGAGGCCAGCGCCGAACTCGGCAATCACGATACCTACACCGCTGACGCCGCCGCCGGCCT
TTCTTCCGACCGCGCCGCGCTTACCCTGAACGGCGGCTACAGCAGCAGCGACGGCGTTTCGGCCGCTGCTTCGGGTACCG
AACCCGACGGCTTCCGCCAGTGGCGCGTCGGCGGCCGGGGCCGCGTGAACCTCACCGACGAGATCGCCGTTCTCGCCTCG
GCCCGCTATGCCGACACGCGCACCGACATCGACGGCTTCGGTCCGCCGACCTACGTGGAATTCGGCGATACGCCTGAATA
CCAGACGACCCGCCAGGCCTCGGGCCGCGTCGGTCTGCGCTACACGGGCGCCGTGCTTACCCTCAACACTGGCTTCGCCC
TGTCTGACACCAAGCGCGACTACTACGACCCGACCTACTCCGCCGACCCCTCCTACGGCTACAAGGGCCGCTCCGAGCGC
GTCGACCTGACCGGCCGCCTCAACCTCCCCGCCGATTTCACGCTCGATTTCGGCGGCGACAGCGAATGGACGCGCTATTC
CGGCACCTACGACGCGCAGCAGAAGGCCCGGCTCACCAGCGGCCACGCCCTGCTCGGCTGGAGCAGCGCGCGCGCCAGCC
TCGCTGCGGGCGTGCGCGTCGACGATCACAGCCGCTTCGGCACCGCGTGGACCTTCGGCGCCAACGGTACGCTTGAATTG
GTGGACAGCCTGCGCCTGCGCGCCTCCTACGGCGAAGGCTTCAAGGCCCCCACGCTCTTCCAGCTCCTGTCCGACTACGG
CAACGCGGATCTCAGGGCGGAACGCAGCAAGTCCTACGACGCGGGTCTCGAATGGGGCAGCCGCACCGGCAAGCTCCACG
CCGCCGTCACGGTCTTCCGCCGCGACAGCCGCAACCTGATCACCTTCGTCTCCTGCGCCAGCCTCGATGCCTGCGCCACG
CGTCCCTATGGTCTCTACGACAACGTCGGCCTGGCCCGCGCGCAGGGCGTCGAAGCCGAACTCGGCGCCCGCCCGGTCGA
TACGCTCCGCCTCCAGGCCGCCTACACCTACCTCGAAACCGAGAACCGCACCGCCGGCACCGCCAACTTCGGCAATGACC
TGGCGCGCCGTCCCGCCCATGCGCTGACCCTGTCGAGCGACTGGGCCCCTGTTGACTCCGGCCCGCTCGCCGGCTTCACC
CTCGGCGCTGACCTCCGCCTCGTGGGAGACAGCTATGACAACGCGTCGAACACCCGCCGCCTCGATGGCTATGCGCTGAC
CACCGTGCGCGCCAGCTTCCCCCTTACCGAGAACGTCGAACTCTACGGCCGCGTCGAGAACCTTTTCGACGTCGCCTACC
AGACCGTGGCCGACTACGGCACCTGGGGCCGCTCTGCCTTCCTCGGCATTCGCGCCCGCTACTGA

Upstream 100 bases:

>100_bases
GCGGGGTGTACCGGGCGCATGCGACGAGGCGCGCCAATCAAGGCCCGGCGCTCCTCCCGCCATGAACGGCAGTTCCGTTC
ATGGGCCGGGAGTCCAAGTC

Downstream 100 bases:

>100_bases
TGAAGCGGCGTTTCGCAACCATGGCGAAACGCCACCTTCCCCCCTTCCGCATGCGGAAGGGGGCGGGGGAGGGCCTGTTC
CCCCCAACCCCGTTCACCGG

Product: TonB-dependent receptor

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 634; Mature: 634

Protein sequence:

>634_residues
MKKYLLSVSAVAFACPALAQAANDVVVADRRADPAAITVIATGSETLVSRVGQPVTVIAADEIRSIQGPDIARVLERVPG
LALTRNGGPGSFTGVRLRGSDAEQVLVLVDGVRVEDVSAPSGGFDFGTLTPGGIERIDVLRGSNSIVWGSAAIGGVIAVQ
SRELDGIEASAELGNHDTYTADAAAGLSSDRAALTLNGGYSSSDGVSAAASGTEPDGFRQWRVGGRGRVNLTDEIAVLAS
ARYADTRTDIDGFGPPTYVEFGDTPEYQTTRQASGRVGLRYTGAVLTLNTGFALSDTKRDYYDPTYSADPSYGYKGRSER
VDLTGRLNLPADFTLDFGGDSEWTRYSGTYDAQQKARLTSGHALLGWSSARASLAAGVRVDDHSRFGTAWTFGANGTLEL
VDSLRLRASYGEGFKAPTLFQLLSDYGNADLRAERSKSYDAGLEWGSRTGKLHAAVTVFRRDSRNLITFVSCASLDACAT
RPYGLYDNVGLARAQGVEAELGARPVDTLRLQAAYTYLETENRTAGTANFGNDLARRPAHALTLSSDWAPVDSGPLAGFT
LGADLRLVGDSYDNASNTRRLDGYALTTVRASFPLTENVELYGRVENLFDVAYQTVADYGTWGRSAFLGIRARY

Sequences:

>Translated_634_residues
MKKYLLSVSAVAFACPALAQAANDVVVADRRADPAAITVIATGSETLVSRVGQPVTVIAADEIRSIQGPDIARVLERVPG
LALTRNGGPGSFTGVRLRGSDAEQVLVLVDGVRVEDVSAPSGGFDFGTLTPGGIERIDVLRGSNSIVWGSAAIGGVIAVQ
SRELDGIEASAELGNHDTYTADAAAGLSSDRAALTLNGGYSSSDGVSAAASGTEPDGFRQWRVGGRGRVNLTDEIAVLAS
ARYADTRTDIDGFGPPTYVEFGDTPEYQTTRQASGRVGLRYTGAVLTLNTGFALSDTKRDYYDPTYSADPSYGYKGRSER
VDLTGRLNLPADFTLDFGGDSEWTRYSGTYDAQQKARLTSGHALLGWSSARASLAAGVRVDDHSRFGTAWTFGANGTLEL
VDSLRLRASYGEGFKAPTLFQLLSDYGNADLRAERSKSYDAGLEWGSRTGKLHAAVTVFRRDSRNLITFVSCASLDACAT
RPYGLYDNVGLARAQGVEAELGARPVDTLRLQAAYTYLETENRTAGTANFGNDLARRPAHALTLSSDWAPVDSGPLAGFT
LGADLRLVGDSYDNASNTRRLDGYALTTVRASFPLTENVELYGRVENLFDVAYQTVADYGTWGRSAFLGIRARY
>Mature_634_residues
MKKYLLSVSAVAFACPALAQAANDVVVADRRADPAAITVIATGSETLVSRVGQPVTVIAADEIRSIQGPDIARVLERVPG
LALTRNGGPGSFTGVRLRGSDAEQVLVLVDGVRVEDVSAPSGGFDFGTLTPGGIERIDVLRGSNSIVWGSAAIGGVIAVQ
SRELDGIEASAELGNHDTYTADAAAGLSSDRAALTLNGGYSSSDGVSAAASGTEPDGFRQWRVGGRGRVNLTDEIAVLAS
ARYADTRTDIDGFGPPTYVEFGDTPEYQTTRQASGRVGLRYTGAVLTLNTGFALSDTKRDYYDPTYSADPSYGYKGRSER
VDLTGRLNLPADFTLDFGGDSEWTRYSGTYDAQQKARLTSGHALLGWSSARASLAAGVRVDDHSRFGTAWTFGANGTLEL
VDSLRLRASYGEGFKAPTLFQLLSDYGNADLRAERSKSYDAGLEWGSRTGKLHAAVTVFRRDSRNLITFVSCASLDACAT
RPYGLYDNVGLARAQGVEAELGARPVDTLRLQAAYTYLETENRTAGTANFGNDLARRPAHALTLSSDWAPVDSGPLAGFT
LGADLRLVGDSYDNASNTRRLDGYALTTVRASFPLTENVELYGRVENLFDVAYQTVADYGTWGRSAFLGIRARY

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: COG4206

COG function: function code H; Outer membrane cobalamin receptor protein

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790405, Length=662, Percent_Identity=27.7945619335347, Blast_Score=197, Evalue=2e-51,
Organism=Escherichia coli, GI1788478, Length=293, Percent_Identity=26.962457337884, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI1786798, Length=264, Percent_Identity=30.3030303030303, Blast_Score=84, Evalue=2e-17,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531
- InterPro:   IPR010101
- InterPro:   IPR010916
- InterPro:   IPR010917 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 67487; Mature: 67487

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.2 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.2 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKYLLSVSAVAFACPALAQAANDVVVADRRADPAAITVIATGSETLVSRVGQPVTVIAA
CCCCEEEHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEEECCCHHHHHHCCCCEEEEEH
DEIRSIQGPDIARVLERVPGLALTRNGGPGSFTGVRLRGSDAEQVLVLVDGVRVEDVSAP
HHHHCCCCCCHHHHHHHCCCEEEEECCCCCCEEEEEEECCCCCEEEEEEECEEEEECCCC
SGGFDFGTLTPGGIERIDVLRGSNSIVWGSAAIGGVIAVQSRELDGIEASAELGNHDTYT
CCCCCCCCCCCCCCCEEEEEECCCCEEEECCCCCCEEEEECCCCCCCCHHHHCCCCCCEE
ADAAAGLSSDRAALTLNGGYSSSDGVSAAASGTEPDGFRQWRVGGRGRVNLTDEIAVLAS
CHHHCCCCCCCEEEEECCCCCCCCCCEECCCCCCCCCCEEEECCCCCEECCCHHEEEEEC
ARYADTRTDIDGFGPPTYVEFGDTPEYQTTRQASGRVGLRYTGAVLTLNTGFALSDTKRD
CCCCCCCCCCCCCCCCCEEECCCCCCCCCHHHCCCCEEEEEECEEEEEECCEEECCCCCC
YYDPTYSADPSYGYKGRSERVDLTGRLNLPADFTLDFGGDSEWTRYSGTYDAQQKARLTS
CCCCCCCCCCCCCCCCCCCEEEEEEEECCCEEEEEECCCCCCCEEECCCCCHHHHHHHCC
GHALLGWSSARASLAAGVRVDDHSRFGTAWTFGANGTLELVDSLRLRASYGEGFKAPTLF
CCEEEEECHHHHHHHCCCEECCCCCCCCEEEECCCCHHHHHHHHHEEECCCCCCCCHHHH
QLLSDYGNADLRAERSKSYDAGLEWGSRTGKLHAAVTVFRRDSRNLITFVSCASLDACAT
HHHHHCCCCCEEECCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCEEEEEEECCCHHHCC
RPYGLYDNVGLARAQGVEAELGARPVDTLRLQAAYTYLETENRTAGTANFGNDLARRPAH
CCCCCCCCCCCHHHCCCCHHCCCCCHHHEEEEEEEEEEECCCCCCCCCCCCHHHHHCCCE
ALTLSSDWAPVDSGPLAGFTLGADLRLVGDSYDNASNTRRLDGYALTTVRASFPLTENVE
EEEECCCCCCCCCCCCCEEEECCCEEEECCCCCCCCCCEEECCEEEEEEEECCCCCCCCC
LYGRVENLFDVAYQTVADYGTWGRSAFLGIRARY
EEHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECC
>Mature Secondary Structure
MKKYLLSVSAVAFACPALAQAANDVVVADRRADPAAITVIATGSETLVSRVGQPVTVIAA
CCCCEEEHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEEECCCHHHHHHCCCCEEEEEH
DEIRSIQGPDIARVLERVPGLALTRNGGPGSFTGVRLRGSDAEQVLVLVDGVRVEDVSAP
HHHHCCCCCCHHHHHHHCCCEEEEECCCCCCEEEEEEECCCCCEEEEEEECEEEEECCCC
SGGFDFGTLTPGGIERIDVLRGSNSIVWGSAAIGGVIAVQSRELDGIEASAELGNHDTYT
CCCCCCCCCCCCCCCEEEEEECCCCEEEECCCCCCEEEEECCCCCCCCHHHHCCCCCCEE
ADAAAGLSSDRAALTLNGGYSSSDGVSAAASGTEPDGFRQWRVGGRGRVNLTDEIAVLAS
CHHHCCCCCCCEEEEECCCCCCCCCCEECCCCCCCCCCEEEECCCCCEECCCHHEEEEEC
ARYADTRTDIDGFGPPTYVEFGDTPEYQTTRQASGRVGLRYTGAVLTLNTGFALSDTKRD
CCCCCCCCCCCCCCCCCEEECCCCCCCCCHHHCCCCEEEEEECEEEEEECCEEECCCCCC
YYDPTYSADPSYGYKGRSERVDLTGRLNLPADFTLDFGGDSEWTRYSGTYDAQQKARLTS
CCCCCCCCCCCCCCCCCCCEEEEEEEECCCEEEEEECCCCCCCEEECCCCCHHHHHHHCC
GHALLGWSSARASLAAGVRVDDHSRFGTAWTFGANGTLELVDSLRLRASYGEGFKAPTLF
CCEEEEECHHHHHHHCCCEECCCCCCCCEEEECCCCHHHHHHHHHEEECCCCCCCCHHHH
QLLSDYGNADLRAERSKSYDAGLEWGSRTGKLHAAVTVFRRDSRNLITFVSCASLDACAT
HHHHHCCCCCEEECCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCEEEEEEECCCHHHCC
RPYGLYDNVGLARAQGVEAELGARPVDTLRLQAAYTYLETENRTAGTANFGNDLARRPAH
CCCCCCCCCCCHHHCCCCHHCCCCCHHHEEEEEEEEEEECCCCCCCCCCCCHHHHHCCCE
ALTLSSDWAPVDSGPLAGFTLGADLRLVGDSYDNASNTRRLDGYALTTVRASFPLTENVE
EEEECCCCCCCCCCCCCEEEECCCEEEECCCCCCCCCCEEECCEEEEEEEECCCCCCCCC
LYGRVENLFDVAYQTVADYGTWGRSAFLGIRARY
EEHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11598060 [H]