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The map label for this gene is garR [C]

Identifier: 87199120

GI number: 87199120

Start: 1140439

End: 1141377

Strand: Reverse

Name: garR [C]

Synonym: Saro_1098

Alternate gene names: 87199120

Gene position: 1141377-1140439 (Counterclockwise)

Preceding gene: 87199126

Following gene: 87199119

Centisome position: 32.05

GC content: 69.01

Gene sequence:

>939_bases
ATGACTGACCAGACTTCCGCTGAACTTTCCGCCCAGGTCCCCATCGAGCCCGCCACCGTCGCCTTCCTCGGCCTTGGCGT
GATGGGCGGCGCGATGGCGCGCCACCTCGGCAAGGCCGGCCACCGGCTGACGATCTACAACCGCACGCCCTCGCGGGCAG
AGGCATGGCAGTCCGCCAATCCGGGCCTTGCCTCTCGCGTCGCCTCCAGCCCGGCCGAAGCCGCCGAGGATGCGGACGTC
GTGATCACCTGTGTCGGCAACGACGACGACCTTGCCGATGTCGTGCTCAGCCCGAATGGCGCCTTTTCCACGTTGCGCCG
GGGCGCGCTCTACATCGATCACACCACCGTCTCGGCGCGCATTGCCCGCCAGATCGCGGTCGAAGGCCGCGACAAGGAAC
TGCTCTGCGTCGACGCGCCGGTCACGGGCGGCCAGTCGGGCGCCGAGGCCGGCACGCTGGCGATCATGTGCGGCGGCAGC
GCCCGCGCGGTCGACGCCGCGCGCCCGATCCTTGCCGCTTACGCCAAGCGCGTGGTCCACGTCGGCAAGTCCGGCGCGGG
GCAGACCGCGAAGATGGCCAACCAGATGTGCATCGCCGGGACGCTTGCCGGCCTGTCCGAGGCGATCCGGTTCGCACAGA
TCGCGCACCTCGATCTCGAAAAGGTGTTCGAGGCGATCTCGGGCGGCGCGGCCCAGTCGTGGCAGATGGACAACCGCTTC
CACACGATGGCGAACGACGAGTTCGACTTCGGCTTCGCGGTCGACTGGATGCGCAAGGACCTTGGCCTGGCCATAGAGGA
GGCGCGCAACCTGGGAGCGAGCGTCCCCGTCACCGCGCTTGTCGACCAGTTCTATGTCGACGTGCAGGCCATGGGGCACG
GCCGCGAGGACACGAGCGCGCTCATCCGCCATTTGCCGCGACGGGCTCCAAGGGCATGA

Upstream 100 bases:

>100_bases
CTGTGTCATCCGGTCCATCGCGCCCTCTATGCTGCGGGGCAATTTCGCTGACAACCCGTACGGGGTGGCGCACCGCCGCA
AACCGGATATGAATGCCTGC

Downstream 100 bases:

>100_bases
TCGTCGGTCGGGCGGCGCTCGCCGCGCTTCTGCTCGCCTCGTCCTCGTCAGTATCCGCGCCCGCACTTGCCGATACCCTG
GTCGACAACGTCAGGGGCGA

Product: 3-hydroxyisobutyrate dehydrogenase

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 312; Mature: 311

Protein sequence:

>312_residues
MTDQTSAELSAQVPIEPATVAFLGLGVMGGAMARHLGKAGHRLTIYNRTPSRAEAWQSANPGLASRVASSPAEAAEDADV
VITCVGNDDDLADVVLSPNGAFSTLRRGALYIDHTTVSARIARQIAVEGRDKELLCVDAPVTGGQSGAEAGTLAIMCGGS
ARAVDAARPILAAYAKRVVHVGKSGAGQTAKMANQMCIAGTLAGLSEAIRFAQIAHLDLEKVFEAISGGAAQSWQMDNRF
HTMANDEFDFGFAVDWMRKDLGLAIEEARNLGASVPVTALVDQFYVDVQAMGHGREDTSALIRHLPRRAPRA

Sequences:

>Translated_312_residues
MTDQTSAELSAQVPIEPATVAFLGLGVMGGAMARHLGKAGHRLTIYNRTPSRAEAWQSANPGLASRVASSPAEAAEDADV
VITCVGNDDDLADVVLSPNGAFSTLRRGALYIDHTTVSARIARQIAVEGRDKELLCVDAPVTGGQSGAEAGTLAIMCGGS
ARAVDAARPILAAYAKRVVHVGKSGAGQTAKMANQMCIAGTLAGLSEAIRFAQIAHLDLEKVFEAISGGAAQSWQMDNRF
HTMANDEFDFGFAVDWMRKDLGLAIEEARNLGASVPVTALVDQFYVDVQAMGHGREDTSALIRHLPRRAPRA
>Mature_311_residues
TDQTSAELSAQVPIEPATVAFLGLGVMGGAMARHLGKAGHRLTIYNRTPSRAEAWQSANPGLASRVASSPAEAAEDADVV
ITCVGNDDDLADVVLSPNGAFSTLRRGALYIDHTTVSARIARQIAVEGRDKELLCVDAPVTGGQSGAEAGTLAIMCGGSA
RAVDAARPILAAYAKRVVHVGKSGAGQTAKMANQMCIAGTLAGLSEAIRFAQIAHLDLEKVFEAISGGAAQSWQMDNRFH
TMANDEFDFGFAVDWMRKDLGLAIEEARNLGASVPVTALVDQFYVDVQAMGHGREDTSALIRHLPRRAPRA

Specific function: D-galactarate metabolism; third step. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=294, Percent_Identity=30.2721088435374, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI40556376, Length=286, Percent_Identity=25.8741258741259, Blast_Score=117, Evalue=1e-26,
Organism=Escherichia coli, GI145693186, Length=290, Percent_Identity=34.1379310344828, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1786719, Length=290, Percent_Identity=32.0689655172414, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI1790315, Length=293, Percent_Identity=31.3993174061433, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI1789092, Length=287, Percent_Identity=28.9198606271777, Blast_Score=97, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17557316, Length=272, Percent_Identity=27.2058823529412, Blast_Score=98, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24655230, Length=302, Percent_Identity=29.4701986754967, Blast_Score=125, Evalue=3e-29,
Organism=Drosophila melanogaster, GI19922568, Length=302, Percent_Identity=29.4701986754967, Blast_Score=125, Evalue=3e-29,
Organism=Drosophila melanogaster, GI28574115, Length=292, Percent_Identity=25, Blast_Score=106, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- InterPro:   IPR006183 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.31

Molecular weight: Translated: 32824; Mature: 32692

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDQTSAELSAQVPIEPATVAFLGLGVMGGAMARHLGKAGHRLTIYNRTPSRAEAWQSAN
CCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHCCCC
PGLASRVASSPAEAAEDADVVITCVGNDDDLADVVLSPNGAFSTLRRGALYIDHTTVSAR
CCHHHHHHCCCHHHCCCCCEEEEEECCCCCCEEEEECCCCHHHHHHCCEEEEECCHHHHH
IARQIAVEGRDKELLCVDAPVTGGQSGAEAGTLAIMCGGSARAVDAARPILAAYAKRVVH
HHHHHHHCCCCCEEEEEECCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHH
VGKSGAGQTAKMANQMCIAGTLAGLSEAIRFAQIAHLDLEKVFEAISGGAAQSWQMDNRF
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCE
HTMANDEFDFGFAVDWMRKDLGLAIEEARNLGASVPVTALVDQFYVDVQAMGHGREDTSA
ECCCCCCCCCCHHHHHHHHHHCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHH
LIRHLPRRAPRA
HHHHHHHCCCCC
>Mature Secondary Structure 
TDQTSAELSAQVPIEPATVAFLGLGVMGGAMARHLGKAGHRLTIYNRTPSRAEAWQSAN
CCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHCCCC
PGLASRVASSPAEAAEDADVVITCVGNDDDLADVVLSPNGAFSTLRRGALYIDHTTVSAR
CCHHHHHHCCCHHHCCCCCEEEEEECCCCCCEEEEECCCCHHHHHHCCEEEEECCHHHHH
IARQIAVEGRDKELLCVDAPVTGGQSGAEAGTLAIMCGGSARAVDAARPILAAYAKRVVH
HHHHHHHCCCCCEEEEEECCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHH
VGKSGAGQTAKMANQMCIAGTLAGLSEAIRFAQIAHLDLEKVFEAISGGAAQSWQMDNRF
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCE
HTMANDEFDFGFAVDWMRKDLGLAIEEARNLGASVPVTALVDQFYVDVQAMGHGREDTSA
ECCCCCCCCCCHHHHHHHHHHCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHH
LIRHLPRRAPRA
HHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA