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The map label for this gene is rnhA

Identifier: 87199107

GI number: 87199107

Start: 1128544

End: 1128975

Strand: Reverse

Name: rnhA

Synonym: Saro_1085

Alternate gene names: 87199107

Gene position: 1128975-1128544 (Counterclockwise)

Preceding gene: 87199108

Following gene: 87199103

Centisome position: 31.7

GC content: 66.2

Gene sequence:

>432_bases
GTGAAGCACGTCGAGATCTTCACCGACGGCGCGTGCAAGGGCAATCCCGGCAAGGGCGGCTGGGGCGCGCTGCTGCGCAT
GGGCGAGCACGAGAAGGAAATGGCCGGCTCCGAAAAGGAGACGACCAACAACCGCATGGAGCTGATGGCTGCGATCCGCG
CGCTCGAGGCGCTGAAGCAGCCCTGCCGCGTGACGCTGCACACCGACAGCAAGTACGTGCTCGACGGCATCACCAAGTGG
ATCTTCGGCTGGCAGAAGAAGGGCTGGAAGACGGCCGACAACAAGCCGGTCAAGAACGAGGACCTGTGGCGCGCGCTGGT
CGATGCGGTGCGCCCGCACAAGGTCGAATGGGTCTGGGTCAAGGGGCACGACGGCCACCCCGAGAACGAGCGGGTCGACA
AGCTAGCGAGCGATGCGGCGCTGGCGGCCTGA

Upstream 100 bases:

>100_bases
CGGCCGACGCGCTGGTGGTGCGCAAGGACCCGATGGCGTTCGCCCGGCGGCTGCAATTCTACGCCGCCAATCCCGCCATA
TTCGACACGAATGCTTTCGC

Downstream 100 bases:

>100_bases
AAGTTCCGCCTGCTGATTTCCGCCTGCTCCGGCACTTCGCTGATGCCGAAGCTGCCTTGGCATCGCGAGTGGCGGCAGCG
GGATCGGGCGGTGCTTCAGT

Product: ribonuclease H

Products: NA

Alternate protein names: RNase H

Number of amino acids: Translated: 143; Mature: 143

Protein sequence:

>143_residues
MKHVEIFTDGACKGNPGKGGWGALLRMGEHEKEMAGSEKETTNNRMELMAAIRALEALKQPCRVTLHTDSKYVLDGITKW
IFGWQKKGWKTADNKPVKNEDLWRALVDAVRPHKVEWVWVKGHDGHPENERVDKLASDAALAA

Sequences:

>Translated_143_residues
MKHVEIFTDGACKGNPGKGGWGALLRMGEHEKEMAGSEKETTNNRMELMAAIRALEALKQPCRVTLHTDSKYVLDGITKW
IFGWQKKGWKTADNKPVKNEDLWRALVDAVRPHKVEWVWVKGHDGHPENERVDKLASDAALAA
>Mature_143_residues
MKHVEIFTDGACKGNPGKGGWGALLRMGEHEKEMAGSEKETTNNRMELMAAIRALEALKQPCRVTLHTDSKYVLDGITKW
IFGWQKKGWKTADNKPVKNEDLWRALVDAVRPHKVEWVWVKGHDGHPENERVDKLASDAALAA

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain

Homologues:

Organism=Homo sapiens, GI21359816, Length=148, Percent_Identity=35.8108108108108, Blast_Score=81, Evalue=3e-16,
Organism=Escherichia coli, GI1786408, Length=141, Percent_Identity=63.1205673758865, Blast_Score=185, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI115532542, Length=140, Percent_Identity=35, Blast_Score=83, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17505482, Length=131, Percent_Identity=35.1145038167939, Blast_Score=67, Evalue=3e-12,
Organism=Drosophila melanogaster, GI45552509, Length=144, Percent_Identity=33.3333333333333, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI17137476, Length=144, Percent_Identity=33.3333333333333, Blast_Score=79, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH_NOVAD (Q2G9E3)

Other databases:

- EMBL:   CP000248
- RefSeq:   YP_496364.1
- ProteinModelPortal:   Q2G9E3
- SMR:   Q2G9E3
- STRING:   Q2G9E3
- GeneID:   3916381
- GenomeReviews:   CP000248_GR
- KEGG:   nar:Saro_1085
- NMPDR:   fig|48935.1.peg.1280
- eggNOG:   COG0328
- HOGENOM:   HBG742437
- OMA:   NGWRTAD
- PhylomeDB:   Q2G9E3
- ProtClustDB:   PRK00203
- BioCyc:   NARO279238:SARO_1085-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00042
- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156

Pfam domain/function: PF00075 RnaseH; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 16086; Mature: 16086

Theoretical pI: Translated: 8.74; Mature: 8.74

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHVEIFTDGACKGNPGKGGWGALLRMGEHEKEMAGSEKETTNNRMELMAAIRALEALKQ
CCCEEEEECCCCCCCCCCCHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHCC
PCRVTLHTDSKYVLDGITKWIFGWQKKGWKTADNKPVKNEDLWRALVDAVRPHKVEWVWV
CCEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEE
KGHDGHPENERVDKLASDAALAA
ECCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKHVEIFTDGACKGNPGKGGWGALLRMGEHEKEMAGSEKETTNNRMELMAAIRALEALKQ
CCCEEEEECCCCCCCCCCCHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHCC
PCRVTLHTDSKYVLDGITKWIFGWQKKGWKTADNKPVKNEDLWRALVDAVRPHKVEWVWV
CCEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEE
KGHDGHPENERVDKLASDAALAA
ECCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA