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The map label for this gene is nadE

Identifier: 87198992

GI number: 87198992

Start: 1015433

End: 1017154

Strand: Reverse

Name: nadE

Synonym: Saro_0970

Alternate gene names: 87198992

Gene position: 1017154-1015433 (Counterclockwise)

Preceding gene: 87198994

Following gene: 87198991

Centisome position: 28.56

GC content: 65.33

Gene sequence:

>1722_bases
ATGACAGGTTTGCAACTCTTGCGGTGGTCGGGCGTTGAAGCCTACGTAACGCTCATGGTCGATACGCTCAGGATAACCCT
CGCCCAGCTCAACCAGTCGGTTGGCGATCTTGCCGCCAATGTCGCGGCCATGCTCGCCGTGCGCGAAAGGGCGCGGGACG
CCGATCTCATCGTTTTCCCCGAACTGCAACTCATCGGCTACCCGCCGGAAGACCTGATCATGAAGCCCGCGCTGGTCGAA
CGCGCGACGGCGGAACTCGAAAAGCTGGCCAGGGTCACGGCCGACGGGGGGCCAGCGATGCTCGTCGGCTCGGTCTTCGT
GCGCGACGGCGCCCTTCACAATGGCGTCGCGCTGCTCGAGGGCGGCCGCGTCGCCGCCACCCGCTTCAAGTACGAGCTGC
CCAACTACGGCACTTTCGACGAGAAGCGCTATTTCCTGCCCGGCCCCCTGCCGGAACCCGTGTTGTTCAAGGGCGCGATG
CTCGGCCTGCCGATCTGCGAGGACATCTGGCACCCCGACGTTTGCCGCCACCTGGCCGAACTGGGCGCGGAAATCTTCAT
CTGCATCAACGGCAGCCCTTACGAGATCGACAAGGACGTGCTGCGCATCGACGGCGTGGCCAAGCGCCGCGCGATCGATA
CCGGCATCCCGCTTGCCTATGTCAACCGCGTCGGCGGCCAGGACGAGATCGTGTTCGACGGGGCCAGCTTCGTCGTCGGG
CCGGAAGGGGCTGTCTGGGTGCAGATGCCTGATTGGGAAGAATCGGTCGTCACGACCGTGTGGCACCGCAGCGATTTCGG
CGATGCCAACCGCTGGCGGTGCGAGGCCGGCGAAGTCCACGAACTCGCGGAGCATCCCGAGGACATCTACTGCGCCATGG
TCATGGCCCTGCGCGACTATGTGAACAAGAACCGCTTCCCCGGTGTGGTCCTCGGCCTCTCGGGCGGCATCGATTCCGCC
ATTTGCGCCGCCATCGCGGTCGACGCGCTTGGCCCTGATCGCGTGTGGTGCGTGATGCTGCCCAGCCGCTACACCTCGCA
GGAAAGCCTGGATGATGCGGCAGGCTGTGCCCGGATGCTCGGCGTGCGCCTCGATACCGTTCCGATCGGCCCCGCCGTGG
ACGCGTTCGACGGCATGCTCGCTCCCGTCTTTGCCGGCCGGCAGCCTGACCTCACCGAAGAGAACATCCAGTCGCGCATC
CGCGGCGTCACGCTCATGGCGCTGTCCAACAAGTTCGGCCCGATGCTCGTCACGACCGGCAACAAGAGCGAGATGAGCGT
CGGCTACGCCACGATCTACGGCGATATGAACGGCGGCTACAATCCCCTCAAGGACGCCTACAAGACCACCGTCTTCGCGG
TGTCGGAGTGGCGCAACTCCCATCGGCCGAAGATCGGGCTCGGCCCCGACGGTCCGGTCATGCCGCAGCGGATCATCACA
AAGCCGCCCAGCGCCGAACTGCGCCCCGACCAGAAGGATTCGGATTCGCTCCCGCCCTACGACGTGCTCGACGCAATTCT
GCAGGGCCTGGTCGAGAACGAGAAGAGCGTGGACCAGATCGTTGCCGAAGGGTTCGAGCGCGATACCGTCGCGCGCATCG
AACGCCTGCTGAACCTTGCCGAATACAAGCGGCGCCAGGCTCCTCCCGGCGTAAAGCTGGGCGCCCGCAACTACGGGCGC
GACCGGCGCTATCCCATCACCCACGGTTTCAGGACAGGTTGA

Upstream 100 bases:

>100_bases
GGCCGAAATGGCGCGGGCGAGCGGCAGATTGGAGTTGCCGGCCATGATCTTCATGCGTGCGATTCCCTCGGATTCGATCC
CCTGCGGCCCTTAGCCCAAG

Downstream 100 bases:

>100_bases
ACAATGCGGCAGGCGTCGAGGTTGAAGGGCGGGGGCTATCTCACATCCACGATCAGCGTGGTTCTTCTGGGGATACCCGC
CCTCAAGTCGGCGCAGGACG

Product: NAD synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 573; Mature: 572

Protein sequence:

>573_residues
MTGLQLLRWSGVEAYVTLMVDTLRITLAQLNQSVGDLAANVAAMLAVRERARDADLIVFPELQLIGYPPEDLIMKPALVE
RATAELEKLARVTADGGPAMLVGSVFVRDGALHNGVALLEGGRVAATRFKYELPNYGTFDEKRYFLPGPLPEPVLFKGAM
LGLPICEDIWHPDVCRHLAELGAEIFICINGSPYEIDKDVLRIDGVAKRRAIDTGIPLAYVNRVGGQDEIVFDGASFVVG
PEGAVWVQMPDWEESVVTTVWHRSDFGDANRWRCEAGEVHELAEHPEDIYCAMVMALRDYVNKNRFPGVVLGLSGGIDSA
ICAAIAVDALGPDRVWCVMLPSRYTSQESLDDAAGCARMLGVRLDTVPIGPAVDAFDGMLAPVFAGRQPDLTEENIQSRI
RGVTLMALSNKFGPMLVTTGNKSEMSVGYATIYGDMNGGYNPLKDAYKTTVFAVSEWRNSHRPKIGLGPDGPVMPQRIIT
KPPSAELRPDQKDSDSLPPYDVLDAILQGLVENEKSVDQIVAEGFERDTVARIERLLNLAEYKRRQAPPGVKLGARNYGR
DRRYPITHGFRTG

Sequences:

>Translated_573_residues
MTGLQLLRWSGVEAYVTLMVDTLRITLAQLNQSVGDLAANVAAMLAVRERARDADLIVFPELQLIGYPPEDLIMKPALVE
RATAELEKLARVTADGGPAMLVGSVFVRDGALHNGVALLEGGRVAATRFKYELPNYGTFDEKRYFLPGPLPEPVLFKGAM
LGLPICEDIWHPDVCRHLAELGAEIFICINGSPYEIDKDVLRIDGVAKRRAIDTGIPLAYVNRVGGQDEIVFDGASFVVG
PEGAVWVQMPDWEESVVTTVWHRSDFGDANRWRCEAGEVHELAEHPEDIYCAMVMALRDYVNKNRFPGVVLGLSGGIDSA
ICAAIAVDALGPDRVWCVMLPSRYTSQESLDDAAGCARMLGVRLDTVPIGPAVDAFDGMLAPVFAGRQPDLTEENIQSRI
RGVTLMALSNKFGPMLVTTGNKSEMSVGYATIYGDMNGGYNPLKDAYKTTVFAVSEWRNSHRPKIGLGPDGPVMPQRIIT
KPPSAELRPDQKDSDSLPPYDVLDAILQGLVENEKSVDQIVAEGFERDTVARIERLLNLAEYKRRQAPPGVKLGARNYGR
DRRYPITHGFRTG
>Mature_572_residues
TGLQLLRWSGVEAYVTLMVDTLRITLAQLNQSVGDLAANVAAMLAVRERARDADLIVFPELQLIGYPPEDLIMKPALVER
ATAELEKLARVTADGGPAMLVGSVFVRDGALHNGVALLEGGRVAATRFKYELPNYGTFDEKRYFLPGPLPEPVLFKGAML
GLPICEDIWHPDVCRHLAELGAEIFICINGSPYEIDKDVLRIDGVAKRRAIDTGIPLAYVNRVGGQDEIVFDGASFVVGP
EGAVWVQMPDWEESVVTTVWHRSDFGDANRWRCEAGEVHELAEHPEDIYCAMVMALRDYVNKNRFPGVVLGLSGGIDSAI
CAAIAVDALGPDRVWCVMLPSRYTSQESLDDAAGCARMLGVRLDTVPIGPAVDAFDGMLAPVFAGRQPDLTEENIQSRIR
GVTLMALSNKFGPMLVTTGNKSEMSVGYATIYGDMNGGYNPLKDAYKTTVFAVSEWRNSHRPKIGLGPDGPVMPQRIITK
PPSAELRPDQKDSDSLPPYDVLDAILQGLVENEKSVDQIVAEGFERDTVARIERLLNLAEYKRRQAPPGVKLGARNYGRD
RRYPITHGFRTG

Specific function: It is not known if this NAD synthase uses NH(3) or glutamine as the N donor [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 62808; Mature: 62677

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGLQLLRWSGVEAYVTLMVDTLRITLAQLNQSVGDLAANVAAMLAVRERARDADLIVFP
CCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEC
ELQLIGYPPEDLIMKPALVERATAELEKLARVTADGGPAMLVGSVFVRDGALHNGVALLE
CEEEECCCCHHHHHCCHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHCCCCCCCCEEEEE
GGRVAATRFKYELPNYGTFDEKRYFLPGPLPEPVLFKGAMLGLPICEDIWHPDVCRHLAE
CCEEEEEEEEEECCCCCCCCCCCEECCCCCCCCHHHCCHHHCCCHHHHCCCHHHHHHHHH
LGAEIFICINGSPYEIDKDVLRIDGVAKRRAIDTGIPLAYVNRVGGQDEIVFDGASFVVG
CCCEEEEEECCCCCCCCHHHHHHCCHHHHHHHCCCCCHHHHHHCCCCCEEEECCCEEEEC
PEGAVWVQMPDWEESVVTTVWHRSDFGDANRWRCEAGEVHELAEHPEDIYCAMVMALRDY
CCCEEEEECCCCHHHHHHHHHCCCCCCCCCCEECCCCCHHHHHCCCHHHHHHHHHHHHHH
VNKNRFPGVVLGLSGGIDSAICAAIAVDALGPDRVWCVMLPSRYTSQESLDDAAGCARML
HCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHH
GVRLDTVPIGPAVDAFDGMLAPVFAGRQPDLTEENIQSRIRGVTLMALSNKFGPMLVTTG
CCEEECCCCCCCHHHHCCHHHHHHCCCCCCCCHHHHHHHHCCEEEEEECCCCCCEEEECC
NKSEMSVGYATIYGDMNGGYNPLKDAYKTTVFAVSEWRNSHRPKIGLGPDGPVMPQRIIT
CCCCCEEEEEEEEECCCCCCCHHHHHHHHHHEEHHHHHHCCCCEECCCCCCCCCCHHHHC
KPPSAELRPDQKDSDSLPPYDVLDAILQGLVENEKSVDQIVAEGFERDTVARIERLLNLA
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHH
EYKRRQAPPGVKLGARNYGRDRRYPITHGFRTG
HHHHHCCCCCCEECCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TGLQLLRWSGVEAYVTLMVDTLRITLAQLNQSVGDLAANVAAMLAVRERARDADLIVFP
CCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEC
ELQLIGYPPEDLIMKPALVERATAELEKLARVTADGGPAMLVGSVFVRDGALHNGVALLE
CEEEECCCCHHHHHCCHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHCCCCCCCCEEEEE
GGRVAATRFKYELPNYGTFDEKRYFLPGPLPEPVLFKGAMLGLPICEDIWHPDVCRHLAE
CCEEEEEEEEEECCCCCCCCCCCEECCCCCCCCHHHCCHHHCCCHHHHCCCHHHHHHHHH
LGAEIFICINGSPYEIDKDVLRIDGVAKRRAIDTGIPLAYVNRVGGQDEIVFDGASFVVG
CCCEEEEEECCCCCCCCHHHHHHCCHHHHHHHCCCCCHHHHHHCCCCCEEEECCCEEEEC
PEGAVWVQMPDWEESVVTTVWHRSDFGDANRWRCEAGEVHELAEHPEDIYCAMVMALRDY
CCCEEEEECCCCHHHHHHHHHCCCCCCCCCCEECCCCCHHHHHCCCHHHHHHHHHHHHHH
VNKNRFPGVVLGLSGGIDSAICAAIAVDALGPDRVWCVMLPSRYTSQESLDDAAGCARML
HCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHH
GVRLDTVPIGPAVDAFDGMLAPVFAGRQPDLTEENIQSRIRGVTLMALSNKFGPMLVTTG
CCEEECCCCCCCHHHHCCHHHHHHCCCCCCCCHHHHHHHHCCEEEEEECCCCCCEEEECC
NKSEMSVGYATIYGDMNGGYNPLKDAYKTTVFAVSEWRNSHRPKIGLGPDGPVMPQRIIT
CCCCCEEEEEEEEECCCCCCCHHHHHHHHHHEEHHHHHHCCCCEECCCCCCCCCCHHHHC
KPPSAELRPDQKDSDSLPPYDVLDAILQGLVENEKSVDQIVAEGFERDTVARIERLLNLA
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHH
EYKRRQAPPGVKLGARNYGRDRRYPITHGFRTG
HHHHHCCCCCCEECCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8431308; 8195100 [H]