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The map label for this gene is 87198824

Identifier: 87198824

GI number: 87198824

Start: 852328

End: 853812

Strand: Reverse

Name: 87198824

Synonym: Saro_0802

Alternate gene names: NA

Gene position: 853812-852328 (Counterclockwise)

Preceding gene: 87198829

Following gene: 87198823

Centisome position: 23.97

GC content: 63.84

Gene sequence:

>1485_bases
ATGGCCCAATTTCCGAACACCCCCAGCTTCACGGGATTCAACACGCCGTCGCGGATCGAGGCGGATATCGCCGATCTGGC
CCACGAAGGCACGATTCCGCAAGGGTTAAACGGCGCATTCTACCGCGTCCAGCCCGACCCGCAGTTTCCTCCCCGCCTCG
ACGACGACATCGCCTTCAACGGCGACGGCATGATCACCCGCTTCCACATCCACGACGGCCAGGTCGACTTCCGCCAGCGC
TGGGCGAAGACCGACAAGTGGAAGCTGGAGAACGCCGCCGGAAAGGCCCTGTTCGGCGCCTACCGCAACCCGCTGACCGA
CGACGAGGCGGTCAAGGGCGAGATCCGTTCGACCGCCAACACCAACGCCTTCGTGTTCGGCGGCAAGCTGTGGGCGATGA
AGGAGGACAGTCCCGCCCTCGTCATGGACCCGGCGACGATGGAAACCTTCGGGTTCGAGAAGTTCGGCGGCAAGATGACC
GGCCAGACCTTTACCGCCCACCCCAAGGTCGATCCGAAGACCGGCAACATGGTCGCCATCGGCTATGCCGCAAGCGGGCT
GTGCACCGACGATGTGACCTACATGGAAGTGAGCCCGGAGGGCGAGCTTGTCCGCGAAGTGTGGTTCAAGGTGCCGTACT
ACTGCATGATGCACGACTTCGGCATCACCGAGGATTACCTCGTGCTGCACATCGTGCCTTCCATCGGAAGCTGGGAAAGG
CTGGAACAGGGCAAGCCGCACTTCGGCTTCGACACGACCATGCCGGTGCACCTCGGCATCATCCCGCGCCGCGACGGCGT
GCGCCAGGAAGACATCCGCTGGTTCACGCGGGACAACTGCTTTGCCAGCCATGTCCTGAACGCCTGGCAAGAGGGGACCA
AGATCCACTTCGTGACCTGCGAGGCGAAGAACAACATGTTCCCGTTCTTCCCCGACGTCCACGGCGCGCCCTTCAACGGC
ATGGAGGCCATGAGCCATCCGACCGACTGGGTGGTCGACATGGCCAGCAACGGCGAGGACTTTGCCGGGATCGTGAAGCT
TTCCGACACAGCCGCCGAGTTCCCGCGCATCGACGACCGCTTTACCGGCCAGAAGACCCGCCATGGCTGGTTCCTCGAAA
TGGACATGAAGCGCCCGGTGGAATTGCGCGGCGGCAGCGCCGGCGGCCTGCTGATGAACTGCCTGTTCCACAAGGACTTC
GAAACGGGTCGCGAGCAGCACTGGTGGTGCGGCCCGGTGTCGAGCCTTCAGGAGCCGTGCTTCGTGCCGCGCGCCAAGGA
TGCCCCCGAAGGCGACGGCTGGATCGTGCAGGTTTGCAACCGGCTGGAAGAGCAGCGCAGCGACTTGCTGATCTTCGACG
CGCTCGACATCGAGAAAGGCCCGGTGGCCACGGTCAACATCCCCATCCGCCTGCGCTTCGGCCTTCACGGCAACTGGGCG
AATGCCGACGAAATCGGCCTTGCCGAGAAGGTCCTGGCCGCATGA

Upstream 100 bases:

>100_bases
GCGATGGCGTCGGCGAATTTGTCCTTGATCAATGCCCTGCCCCCAACGAATGCGCTTGTCTAATTGTTAGCAATACATAC
AATATTGCAAGGAGGATGCC

Downstream 100 bases:

>100_bases
TCCAGCCCGGAGCGGTGCGGCCCGGCCTCGAGTTCGTCTACGAAGCGACCGGCGACCTCGAGCCGCCGGTCGCGATCGGC
GAGACCTATGACGGGACGCG

Product: carotenoid oxygenase

Products: NA

Alternate protein names: ACO; 8'-apo-beta-carotenal 15,15'-oxygenase; Diox1 [H]

Number of amino acids: Translated: 494; Mature: 493

Protein sequence:

>494_residues
MAQFPNTPSFTGFNTPSRIEADIADLAHEGTIPQGLNGAFYRVQPDPQFPPRLDDDIAFNGDGMITRFHIHDGQVDFRQR
WAKTDKWKLENAAGKALFGAYRNPLTDDEAVKGEIRSTANTNAFVFGGKLWAMKEDSPALVMDPATMETFGFEKFGGKMT
GQTFTAHPKVDPKTGNMVAIGYAASGLCTDDVTYMEVSPEGELVREVWFKVPYYCMMHDFGITEDYLVLHIVPSIGSWER
LEQGKPHFGFDTTMPVHLGIIPRRDGVRQEDIRWFTRDNCFASHVLNAWQEGTKIHFVTCEAKNNMFPFFPDVHGAPFNG
MEAMSHPTDWVVDMASNGEDFAGIVKLSDTAAEFPRIDDRFTGQKTRHGWFLEMDMKRPVELRGGSAGGLLMNCLFHKDF
ETGREQHWWCGPVSSLQEPCFVPRAKDAPEGDGWIVQVCNRLEEQRSDLLIFDALDIEKGPVATVNIPIRLRFGLHGNWA
NADEIGLAEKVLAA

Sequences:

>Translated_494_residues
MAQFPNTPSFTGFNTPSRIEADIADLAHEGTIPQGLNGAFYRVQPDPQFPPRLDDDIAFNGDGMITRFHIHDGQVDFRQR
WAKTDKWKLENAAGKALFGAYRNPLTDDEAVKGEIRSTANTNAFVFGGKLWAMKEDSPALVMDPATMETFGFEKFGGKMT
GQTFTAHPKVDPKTGNMVAIGYAASGLCTDDVTYMEVSPEGELVREVWFKVPYYCMMHDFGITEDYLVLHIVPSIGSWER
LEQGKPHFGFDTTMPVHLGIIPRRDGVRQEDIRWFTRDNCFASHVLNAWQEGTKIHFVTCEAKNNMFPFFPDVHGAPFNG
MEAMSHPTDWVVDMASNGEDFAGIVKLSDTAAEFPRIDDRFTGQKTRHGWFLEMDMKRPVELRGGSAGGLLMNCLFHKDF
ETGREQHWWCGPVSSLQEPCFVPRAKDAPEGDGWIVQVCNRLEEQRSDLLIFDALDIEKGPVATVNIPIRLRFGLHGNWA
NADEIGLAEKVLAA
>Mature_493_residues
AQFPNTPSFTGFNTPSRIEADIADLAHEGTIPQGLNGAFYRVQPDPQFPPRLDDDIAFNGDGMITRFHIHDGQVDFRQRW
AKTDKWKLENAAGKALFGAYRNPLTDDEAVKGEIRSTANTNAFVFGGKLWAMKEDSPALVMDPATMETFGFEKFGGKMTG
QTFTAHPKVDPKTGNMVAIGYAASGLCTDDVTYMEVSPEGELVREVWFKVPYYCMMHDFGITEDYLVLHIVPSIGSWERL
EQGKPHFGFDTTMPVHLGIIPRRDGVRQEDIRWFTRDNCFASHVLNAWQEGTKIHFVTCEAKNNMFPFFPDVHGAPFNGM
EAMSHPTDWVVDMASNGEDFAGIVKLSDTAAEFPRIDDRFTGQKTRHGWFLEMDMKRPVELRGGSAGGLLMNCLFHKDFE
TGREQHWWCGPVSSLQEPCFVPRAKDAPEGDGWIVQVCNRLEEQRSDLLIFDALDIEKGPVATVNIPIRLRFGLHGNWAN
ADEIGLAEKVLAA

Specific function: Cleaves a number of carotenals and carotenols in the all-trans configuration at the 15-15' double bond producing retinal or retinol, respectively. Also shows activity toward lycopenals and the corresponding alcohols. Does not cleave beta- carotene or lyco

COG id: COG3670

COG function: function code Q; Lignostilbene-alpha,beta-dioxygenase and related enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carotenoid oxygenase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004294 [H]

Pfam domain/function: PF03055 RPE65 [H]

EC number: =1.14.99.41 [H]

Molecular weight: Translated: 55421; Mature: 55290

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQFPNTPSFTGFNTPSRIEADIADLAHEGTIPQGLNGAFYRVQPDPQFPPRLDDDIAFN
CCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCCCEEEC
GDGMITRFHIHDGQVDFRQRWAKTDKWKLENAAGKALFGAYRNPLTDDEAVKGEIRSTAN
CCCEEEEEEEECCCCHHHHHHCCCCCEEECCCCCHHHHHHHCCCCCCCHHHHHHHHCCCC
TNAFVFGGKLWAMKEDSPALVMDPATMETFGFEKFGGKMTGQTFTAHPKVDPKTGNMVAI
CCEEEEECEEEEEECCCCEEEECCCCHHHCCHHHHCCEECCCEEEECCCCCCCCCCEEEE
GYAASGLCTDDVTYMEVSPEGELVREVWFKVPYYCMMHDFGITEDYLVLHIVPSIGSWER
EEECCCCCCCCEEEEEECCCHHHHHHHHHHCCEEEEEEECCCCCCEEEEEEECCCCCHHH
LEQGKPHFGFDTTMPVHLGIIPRRDGVRQEDIRWFTRDNCFASHVLNAWQEGTKIHFVTC
HHCCCCCCCCCCCCCEEEEEEECCCCCCHHHCEEEECCCHHHHHHHHHHHCCCEEEEEEE
EAKNNMFPFFPDVHGAPFNGMEAMSHPTDWVVDMASNGEDFAGIVKLSDTAAEFPRIDDR
ECCCCCCCCCCCCCCCCCCCCHHHCCCCCEEEEECCCCCCEEEEEEECCCHHHCCCCCCC
FTGQKTRHGWFLEMDMKRPVELRGGSAGGLLMNCLFHKDFETGREQHWWCGPVSSLQEPC
CCCCCCCCCEEEEECCCCCEEECCCCCCHHHHHHHHHCCHHCCCCCCEEECCHHHHCCCC
FVPRAKDAPEGDGWIVQVCNRLEEQRSDLLIFDALDIEKGPVATVNIPIRLRFGLHGNWA
CCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEEECCCCCEEEEEEEEEEEEECCCCCC
NADEIGLAEKVLAA
CCHHHCHHHHHHCC
>Mature Secondary Structure 
AQFPNTPSFTGFNTPSRIEADIADLAHEGTIPQGLNGAFYRVQPDPQFPPRLDDDIAFN
CCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCCCEEEC
GDGMITRFHIHDGQVDFRQRWAKTDKWKLENAAGKALFGAYRNPLTDDEAVKGEIRSTAN
CCCEEEEEEEECCCCHHHHHHCCCCCEEECCCCCHHHHHHHCCCCCCCHHHHHHHHCCCC
TNAFVFGGKLWAMKEDSPALVMDPATMETFGFEKFGGKMTGQTFTAHPKVDPKTGNMVAI
CCEEEEECEEEEEECCCCEEEECCCCHHHCCHHHHCCEECCCEEEECCCCCCCCCCEEEE
GYAASGLCTDDVTYMEVSPEGELVREVWFKVPYYCMMHDFGITEDYLVLHIVPSIGSWER
EEECCCCCCCCEEEEEECCCHHHHHHHHHHCCEEEEEEECCCCCCEEEEEEECCCCCHHH
LEQGKPHFGFDTTMPVHLGIIPRRDGVRQEDIRWFTRDNCFASHVLNAWQEGTKIHFVTC
HHCCCCCCCCCCCCCEEEEEEECCCCCCHHHCEEEECCCHHHHHHHHHHHCCCEEEEEEE
EAKNNMFPFFPDVHGAPFNGMEAMSHPTDWVVDMASNGEDFAGIVKLSDTAAEFPRIDDR
ECCCCCCCCCCCCCCCCCCCCHHHCCCCCEEEEECCCCCCEEEEEEECCCHHHCCCCCCC
FTGQKTRHGWFLEMDMKRPVELRGGSAGGLLMNCLFHKDFETGREQHWWCGPVSSLQEPC
CCCCCCCCCEEEEECCCCCEEECCCCCCHHHHHHHHHCCHHCCCCCCEEECCHHHHCCCC
FVPRAKDAPEGDGWIVQVCNRLEEQRSDLLIFDALDIEKGPVATVNIPIRLRFGLHGNWA
CCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEEECCCCCEEEEEEEEEEEEECCCCCC
NADEIGLAEKVLAA
CCHHHCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]