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The map label for this gene is thcD [H]

Identifier: 87198241

GI number: 87198241

Start: 224840

End: 226087

Strand: Direct

Name: thcD [H]

Synonym: Saro_0216

Alternate gene names: 87198241

Gene position: 224840-226087 (Clockwise)

Preceding gene: 87198239

Following gene: 87198243

Centisome position: 6.31

GC content: 67.07

Gene sequence:

>1248_bases
ATGGCCAGCGAAGTTCAGGCAGAGCGTGCAGACGTCGTCATCGTCGGAGCAGGTCACGGCGGAGCCCAGGCGGCCATCGC
GCTGCGCCAGAACGGCTTCGAGGGTCGCGTGCTGGTCATTGGTCGCGAGCCCGAGATCCCTTATGAACGTCCGCCGCTGT
CCAAGGAGTACCTCGCGCGCGAAAAGACGTTCGAGCGCATCTGCATCCGCCCGGCGCAGTTCTGGGAGGACAAGGCGGTC
GAGATGAAGCTCGGCGCAGAGGTCGTCTCGCTCGACCCGGCCGCGCACACGGTCAAGCTGGGCGACGGCTCGGCCATCGA
ATATGGCAAACTGATCTGGGCGACCGGTGGCGATCCGCGGCGGCTGTCCTGCGTGGGTGCCGACCTTGCAGGCGTCCATG
CGGTGCGCACCAAGGAAGACGCCGACCGCCTGATGGCCGAGCTTGATGCCGGCGCGAAGAATGCGGTGGTGATCGGCGGC
GGCTACATCGGGCTGGAAGCGGCGGCGGTCCTGACCAAGTTCGGCGTCAACGTCACCCTGCTGGAAGCCCTTCCGCGCGT
GCTGGCGCGCGTCGCGGGCGAGGCGCTGTCCGAATTCTACCAGGCAGAGCACCGCGCGCACGGCGTCGACCTGCGCACCG
GCGCGGCGATGGACTGCATCGAGGGCGACGGCACCAAGGTGACCGGCGTCCGGATGCAGGACGGCTCGGTCATTCCGGCA
GATATCGTCATCGTCGGCATCGGCATCGTGCCCTGCGTCGGGGCGCTGATCTCGGCGGGAGCATCGGGCGGCAACGGCGT
CGACGTGGACGAGTTCTGCCGTACCTCGCTGACCGACGTCTATGCCATCGGCGATTGCGCTGCCCATGCCAACGACTTTG
CCGACGGCGCCGTGATCCGCCTCGAATCGGTACAGAACGCCAACGACATGGCGACGGCTGCCGCAAAGGACATCTGTGGC
GCCCCGGTGCCCTACAAGGCGACGCCGTGGTTCTGGTCGAACCAGTACGATCTCAAGCTGCAGACGGTCGGCCTTTCCAC
CGGGCACGACAACGCGGTTCTGCGCGGAGACCCCGCGACGCGGTCGTTCTCGGTGGTGTACCTCAAGGGCGGCAAGGTCG
TCGCGCTCGACTGCGTGAACATGGTCAAGGACTATGTTCAGGGCAAGAAGCTGGTCGAAGCCCGCGCGCAGATCGCACCG
GAGCAGCTCGCCGATGCCGGCGTGCCGCTCAAGGAGATGCTGGCCTAG

Upstream 100 bases:

>100_bases
GCGCAGGTATATTGCTTGGCGACTCCGGACTTTTTGACCGCGATCAGTTGACGAAAGCCGACTTTTGGCCAAACCGGCCC
GCACAGATCGGGAGATTCGA

Downstream 100 bases:

>100_bases
GGCCATTCATCTCAGCCAAGTTCCACCAGGGCCCAGCGCGCCGCATCCGCGACTGCGGGGTCGGGATCGGCGAGCAGGTT
GTGCACGACGGGAAGCAATG

Product: FAD-dependent pyridine nucleotide-disulfide oxidoreductase

Products: oxidized ferredoxin; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 415; Mature: 414

Protein sequence:

>415_residues
MASEVQAERADVVIVGAGHGGAQAAIALRQNGFEGRVLVIGREPEIPYERPPLSKEYLAREKTFERICIRPAQFWEDKAV
EMKLGAEVVSLDPAAHTVKLGDGSAIEYGKLIWATGGDPRRLSCVGADLAGVHAVRTKEDADRLMAELDAGAKNAVVIGG
GYIGLEAAAVLTKFGVNVTLLEALPRVLARVAGEALSEFYQAEHRAHGVDLRTGAAMDCIEGDGTKVTGVRMQDGSVIPA
DIVIVGIGIVPCVGALISAGASGGNGVDVDEFCRTSLTDVYAIGDCAAHANDFADGAVIRLESVQNANDMATAAAKDICG
APVPYKATPWFWSNQYDLKLQTVGLSTGHDNAVLRGDPATRSFSVVYLKGGKVVALDCVNMVKDYVQGKKLVEARAQIAP
EQLADAGVPLKEMLA

Sequences:

>Translated_415_residues
MASEVQAERADVVIVGAGHGGAQAAIALRQNGFEGRVLVIGREPEIPYERPPLSKEYLAREKTFERICIRPAQFWEDKAV
EMKLGAEVVSLDPAAHTVKLGDGSAIEYGKLIWATGGDPRRLSCVGADLAGVHAVRTKEDADRLMAELDAGAKNAVVIGG
GYIGLEAAAVLTKFGVNVTLLEALPRVLARVAGEALSEFYQAEHRAHGVDLRTGAAMDCIEGDGTKVTGVRMQDGSVIPA
DIVIVGIGIVPCVGALISAGASGGNGVDVDEFCRTSLTDVYAIGDCAAHANDFADGAVIRLESVQNANDMATAAAKDICG
APVPYKATPWFWSNQYDLKLQTVGLSTGHDNAVLRGDPATRSFSVVYLKGGKVVALDCVNMVKDYVQGKKLVEARAQIAP
EQLADAGVPLKEMLA
>Mature_414_residues
ASEVQAERADVVIVGAGHGGAQAAIALRQNGFEGRVLVIGREPEIPYERPPLSKEYLAREKTFERICIRPAQFWEDKAVE
MKLGAEVVSLDPAAHTVKLGDGSAIEYGKLIWATGGDPRRLSCVGADLAGVHAVRTKEDADRLMAELDAGAKNAVVIGGG
YIGLEAAAVLTKFGVNVTLLEALPRVLARVAGEALSEFYQAEHRAHGVDLRTGAAMDCIEGDGTKVTGVRMQDGSVIPAD
IVIVGIGIVPCVGALISAGASGGNGVDVDEFCRTSLTDVYAIGDCAAHANDFADGAVIRLESVQNANDMATAAAKDICGA
PVPYKATPWFWSNQYDLKLQTVGLSTGHDNAVLRGDPATRSFSVVYLKGGKVVALDCVNMVKDYVQGKKLVEARAQIAPE
QLADAGVPLKEMLA

Specific function: The degradation of the thiocarbamate herbicide EPTC by cytochrome CYP116 (thcB) requires the participation of a flavoprotein, rhodocoxin reductase, and an iron-sulfur protein, rhodocoxin, to mediate the transfer of electrons from NADH to P450 for oxygen a

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI21389617, Length=377, Percent_Identity=30.2387267904509, Blast_Score=182, Evalue=6e-46,
Organism=Homo sapiens, GI65787454, Length=377, Percent_Identity=30.2387267904509, Blast_Score=182, Evalue=7e-46,
Organism=Homo sapiens, GI226437568, Length=377, Percent_Identity=30.2387267904509, Blast_Score=181, Evalue=8e-46,
Organism=Homo sapiens, GI4757732, Length=346, Percent_Identity=28.9017341040462, Blast_Score=105, Evalue=6e-23,
Organism=Homo sapiens, GI22202629, Length=346, Percent_Identity=28.9017341040462, Blast_Score=105, Evalue=7e-23,
Organism=Homo sapiens, GI195927006, Length=162, Percent_Identity=35.8024691358025, Blast_Score=81, Evalue=2e-15,
Organism=Escherichia coli, GI1788892, Length=402, Percent_Identity=32.8358208955224, Blast_Score=162, Evalue=3e-41,
Organism=Escherichia coli, GI1789065, Length=317, Percent_Identity=25.8675078864353, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1789765, Length=258, Percent_Identity=27.1317829457364, Blast_Score=71, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17559934, Length=376, Percent_Identity=30.3191489361702, Blast_Score=152, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI32564386, Length=359, Percent_Identity=27.0194986072423, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24585130, Length=339, Percent_Identity=27.4336283185841, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24639257, Length=327, Percent_Identity=29.6636085626911, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI281359715, Length=328, Percent_Identity=30.1829268292683, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI281359713, Length=328, Percent_Identity=30.1829268292683, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24639250, Length=328, Percent_Identity=30.1829268292683, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI18543267, Length=328, Percent_Identity=30.1829268292683, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24639252, Length=328, Percent_Identity=30.1829268292683, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI28573993, Length=377, Percent_Identity=26.525198938992, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24581020, Length=377, Percent_Identity=26.525198938992, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3

Molecular weight: Translated: 43636; Mature: 43505

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASEVQAERADVVIVGAGHGGAQAAIALRQNGFEGRVLVIGREPEIPYERPPLSKEYLAR
CCCCCCCCCCCEEEEECCCCCCCEEEEEECCCCCCEEEEEECCCCCCCCCCCCCHHHHHH
EKTFERICIRPAQFWEDKAVEMKLGAEVVSLDPAAHTVKLGDGSAIEYGKLIWATGGDPR
HHHHHHHHCCCHHHCCCCCEEEEECCEEEEECCCCEEEEECCCCCEEECCEEEECCCCCC
RLSCVGADLAGVHAVRTKEDADRLMAELDAGAKNAVVIGGGYIGLEAAAVLTKFGVNVTL
EEEEECCCHHCEEEECCHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHCCCCHH
LEALPRVLARVAGEALSEFYQAEHRAHGVDLRTGAAMDCIEGDGTKVTGVRMQDGSVIPA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCEEECCCCCEEEEEEECCCCCCCH
DIVIVGIGIVPCVGALISAGASGGNGVDVDEFCRTSLTDVYAIGDCAAHANDFADGAVIR
HHEEEHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHEEEECHHHHCCCCCCCCEEEE
LESVQNANDMATAAAKDICGAPVPYKATPWFWSNQYDLKLQTVGLSTGHDNAVLRGDPAT
EECCCCCHHHHHHHHHHHCCCCCCCCCCCCEECCCEEEEEEEEEECCCCCCCEEECCCCC
RSFSVVYLKGGKVVALDCVNMVKDYVQGKKLVEARAQIAPEQLADAGVPLKEMLA
CEEEEEEEECCCEEEEHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHC
>Mature Secondary Structure 
ASEVQAERADVVIVGAGHGGAQAAIALRQNGFEGRVLVIGREPEIPYERPPLSKEYLAR
CCCCCCCCCCEEEEECCCCCCCEEEEEECCCCCCEEEEEECCCCCCCCCCCCCHHHHHH
EKTFERICIRPAQFWEDKAVEMKLGAEVVSLDPAAHTVKLGDGSAIEYGKLIWATGGDPR
HHHHHHHHCCCHHHCCCCCEEEEECCEEEEECCCCEEEEECCCCCEEECCEEEECCCCCC
RLSCVGADLAGVHAVRTKEDADRLMAELDAGAKNAVVIGGGYIGLEAAAVLTKFGVNVTL
EEEEECCCHHCEEEECCHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHCCCCHH
LEALPRVLARVAGEALSEFYQAEHRAHGVDLRTGAAMDCIEGDGTKVTGVRMQDGSVIPA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCEEECCCCCEEEEEEECCCCCCCH
DIVIVGIGIVPCVGALISAGASGGNGVDVDEFCRTSLTDVYAIGDCAAHANDFADGAVIR
HHEEEHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHEEEECHHHHCCCCCCCCEEEE
LESVQNANDMATAAAKDICGAPVPYKATPWFWSNQYDLKLQTVGLSTGHDNAVLRGDPAT
EECCCCCHHHHHHHHHHHCCCCCCCCCCCCEECCCEEEEEEEEEECCCCCCCEEECCCCC
RSFSVVYLKGGKVVALDCVNMVKDYVQGKKLVEARAQIAPEQLADAGVPLKEMLA
CEEEEEEEECCCEEEEHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced ferredoxin; NAD+

Specific reaction: reduced ferredoxin + NAD+ = oxidized ferredoxin + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]