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The map label for this gene is rho

Identifier: 87198146

GI number: 87198146

Start: 121800

End: 123056

Strand: Direct

Name: rho

Synonym: Saro_0120

Alternate gene names: 87198146

Gene position: 121800-123056 (Clockwise)

Preceding gene: 87198145

Following gene: 87198147

Centisome position: 3.42

GC content: 60.78

Gene sequence:

>1257_bases
ATGCATCTCAAAGACCTCAAGAAGAAGACCCCCGCCGAGCTGGTCCAGATGGCCGAAGAGCTCGAGGTCGAAGGCGCCAG
CACCATGCGTCGCCAGGACCTGATGTTCGCTATCCTCAAGGAAATGGCCGAAGACGGCGAGGAAATCCTCGGCATCGGCA
CGATCGAGGTTCTTCCCGACGGTTTCGGCTTCCTGCGGAGCCCCGAAGCGAACTATCTCGCCGGACCCGACGATATCTAC
GTCTCGCCGAACCAGGTCCGCAAATGGGGCCTGCGCACCGGCGACACGGTGGAAGGCGAAGTCCGCGCGCCCAAGGACGG
GGAGCGCTATTTCGCGATCACCCGTCTGATCAAGGTGAACTTCGACGATCCCGAGGCCGTGCGCCACCGTGTCAACTTCG
ACAACCTGACCCCGCTCTATCCGAACGAGCGACTGAAGCTCGACACGCTCGACCCGACGGTCAAGGACAAGTCGGCTCGT
GTGATCGATCTCGTTTCGCCACAGGGCAAGGGCCAGCGCGCGCTGATCGTCGCCCCTCCGCGCACCGGCAAGACCGTGTT
GCTGCAGAACATGGCCAAGGCGATCACAGACAACCATCCGGAAGTCTTCCTGATCGTGCTTCTGGTTGACGAACGTCCCG
AAGAAGTCACCGACATGCAGCGTTCGGTGAAGGGCGAGGTCATTTCCTCGACCTTTGACGAACCAGCCTCGCGCCACGTC
CAGGTCGCTGAAATGGTCATCGAGAAGGCCAAGCGTCTTGTCGAGCACAAGCGCGACGTGGTGATCCTGCTCGACTCGAT
CACACGTCTCGGCCGTGCGTACAACACCGTCGTGCCCTCGTCGGGCAAGGTGCTGACCGGCGGTGTCGATGCCAACGCCC
TGCAGCGTCCCAAGCGCTTCTTCGGCGCGGCGCGCAACATCGAGGAAGGCGGTTCGCTTTCGATCATTGCCACGGCGCTG
ATCGATACCGGCAGCCGCATGGACGAAGTGATCTTCGAAGAGTTCAAGGGCACCGGCAACTCGGAAATCGTGCTGGACCG
CAAGGTTGCGGACAAGCGCATCTTCCCGGCGCTGGACGTGGGCAAGAGCGGTACCCGCAAGGAAGAACTGCTCGTACCGA
AGGATCAGCTCTCGAAGATGTGGGTCCTGCGCCGCATCTTGATGCAGATGGGCACTGTCGATGCGATGGAGTTCCTGCTC
GACAAGATGAAGGATTCGAAAACCAACGAAGACTTCTTCGCGACGATGAACCAGTAA

Upstream 100 bases:

>100_bases
GCGCTGCCCGCGATGCAGCCGCCCTCTCGCTTTCTCCAAGCCCCATAGAGGCTTCCGGCCATTGACCAGCCTTTTGGCAA
ATCCTTGGAATACAAAGACA

Downstream 100 bases:

>100_bases
GGTTTCGGGTCGAGAGACAAGTAAAAGGCCCGGAGCGATCCGGGCCTTTTTCGTGTTGGCAAACGGCGGGGATAAAATTC
CTGCGGTCGCTTGTTTCAGA

Product: transcription termination factor Rho

Products: NA

Alternate protein names: ATP-dependent helicase Rho [H]

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MHLKDLKKKTPAELVQMAEELEVEGASTMRRQDLMFAILKEMAEDGEEILGIGTIEVLPDGFGFLRSPEANYLAGPDDIY
VSPNQVRKWGLRTGDTVEGEVRAPKDGERYFAITRLIKVNFDDPEAVRHRVNFDNLTPLYPNERLKLDTLDPTVKDKSAR
VIDLVSPQGKGQRALIVAPPRTGKTVLLQNMAKAITDNHPEVFLIVLLVDERPEEVTDMQRSVKGEVISSTFDEPASRHV
QVAEMVIEKAKRLVEHKRDVVILLDSITRLGRAYNTVVPSSGKVLTGGVDANALQRPKRFFGAARNIEEGGSLSIIATAL
IDTGSRMDEVIFEEFKGTGNSEIVLDRKVADKRIFPALDVGKSGTRKEELLVPKDQLSKMWVLRRILMQMGTVDAMEFLL
DKMKDSKTNEDFFATMNQ

Sequences:

>Translated_418_residues
MHLKDLKKKTPAELVQMAEELEVEGASTMRRQDLMFAILKEMAEDGEEILGIGTIEVLPDGFGFLRSPEANYLAGPDDIY
VSPNQVRKWGLRTGDTVEGEVRAPKDGERYFAITRLIKVNFDDPEAVRHRVNFDNLTPLYPNERLKLDTLDPTVKDKSAR
VIDLVSPQGKGQRALIVAPPRTGKTVLLQNMAKAITDNHPEVFLIVLLVDERPEEVTDMQRSVKGEVISSTFDEPASRHV
QVAEMVIEKAKRLVEHKRDVVILLDSITRLGRAYNTVVPSSGKVLTGGVDANALQRPKRFFGAARNIEEGGSLSIIATAL
IDTGSRMDEVIFEEFKGTGNSEIVLDRKVADKRIFPALDVGKSGTRKEELLVPKDQLSKMWVLRRILMQMGTVDAMEFLL
DKMKDSKTNEDFFATMNQ
>Mature_418_residues
MHLKDLKKKTPAELVQMAEELEVEGASTMRRQDLMFAILKEMAEDGEEILGIGTIEVLPDGFGFLRSPEANYLAGPDDIY
VSPNQVRKWGLRTGDTVEGEVRAPKDGERYFAITRLIKVNFDDPEAVRHRVNFDNLTPLYPNERLKLDTLDPTVKDKSAR
VIDLVSPQGKGQRALIVAPPRTGKTVLLQNMAKAITDNHPEVFLIVLLVDERPEEVTDMQRSVKGEVISSTFDEPASRHV
QVAEMVIEKAKRLVEHKRDVVILLDSITRLGRAYNTVVPSSGKVLTGGVDANALQRPKRFFGAARNIEEGGSLSIIATAL
IDTGSRMDEVIFEEFKGTGNSEIVLDRKVADKRIFPALDVGKSGTRKEELLVPKDQLSKMWVLRRILMQMGTVDAMEFLL
DKMKDSKTNEDFFATMNQ

Specific function: Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template [H]

COG id: COG1158

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Rho family [H]

Homologues:

Organism=Escherichia coli, GI1790217, Length=418, Percent_Identity=65.5502392344498, Blast_Score=593, Evalue=1e-171,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000194
- InterPro:   IPR003593
- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR011112
- InterPro:   IPR011113
- InterPro:   IPR004665 [H]

Pfam domain/function: PF00006 ATP-synt_ab; PF07498 Rho_N; PF07497 Rho_RNA_bind [H]

EC number: NA

Molecular weight: Translated: 46774; Mature: 46774

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHLKDLKKKTPAELVQMAEELEVEGASTMRRQDLMFAILKEMAEDGEEILGIGTIEVLPD
CCCHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHEEECCEEEECCC
GFGFLRSPEANYLAGPDDIYVSPNQVRKWGLRTGDTVEGEVRAPKDGERYFAITRLIKVN
CCCCCCCCCCCCCCCCCCEEECHHHHHHCCCCCCCCCCCCEECCCCCCCEEEEEEEEEEC
FDDPEAVRHRVNFDNLTPLYPNERLKLDTLDPTVKDKSARVIDLVSPQGKGQRALIVAPP
CCCHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCCCCCEEEEECCCCCCCEEEEEECC
RTGKTVLLQNMAKAITDNHPEVFLIVLLVDERPEEVTDMQRSVKGEVISSTFDEPASRHV
CCCCHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCHHHHHH
QVAEMVIEKAKRLVEHKRDVVILLDSITRLGRAYNTVVPSSGKVLTGGVDANALQRPKRF
HHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHH
FGAARNIEEGGSLSIIATALIDTGSRMDEVIFEEFKGTGNSEIVLDRKVADKRIFPALDV
HHHHCCCCCCCCEEEEHHHHHHCCCHHHHHHHHHHCCCCCCEEEEECCHHCCCCCCCCCC
GKSGTRKEELLVPKDQLSKMWVLRRILMQMGTVDAMEFLLDKMKDSKTNEDFFATMNQ
CCCCCCCHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCEEECCC
>Mature Secondary Structure
MHLKDLKKKTPAELVQMAEELEVEGASTMRRQDLMFAILKEMAEDGEEILGIGTIEVLPD
CCCHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHEEECCEEEECCC
GFGFLRSPEANYLAGPDDIYVSPNQVRKWGLRTGDTVEGEVRAPKDGERYFAITRLIKVN
CCCCCCCCCCCCCCCCCCEEECHHHHHHCCCCCCCCCCCCEECCCCCCCEEEEEEEEEEC
FDDPEAVRHRVNFDNLTPLYPNERLKLDTLDPTVKDKSARVIDLVSPQGKGQRALIVAPP
CCCHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCCCCCEEEEECCCCCCCEEEEEECC
RTGKTVLLQNMAKAITDNHPEVFLIVLLVDERPEEVTDMQRSVKGEVISSTFDEPASRHV
CCCCHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCHHHHHH
QVAEMVIEKAKRLVEHKRDVVILLDSITRLGRAYNTVVPSSGKVLTGGVDANALQRPKRF
HHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHH
FGAARNIEEGGSLSIIATALIDTGSRMDEVIFEEFKGTGNSEIVLDRKVADKRIFPALDV
HHHHCCCCCCCCEEEEHHHHHHCCCHHHHHHHHHHCCCCCCEEEEECCHHCCCCCCCCCC
GKSGTRKEELLVPKDQLSKMWVLRRILMQMGTVDAMEFLLDKMKDSKTNEDFFATMNQ
CCCCCCCHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8606169 [H]