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The map label for this gene is lepB [H]

Identifier: 86751691

GI number: 86751691

Start: 5187438

End: 5188208

Strand: Direct

Name: lepB [H]

Synonym: RPB_4593

Alternate gene names: 86751691

Gene position: 5187438-5188208 (Clockwise)

Preceding gene: 86751690

Following gene: 86751700

Centisome position: 97.3

GC content: 68.22

Gene sequence:

>771_bases
ATGAACGGCAAGGCAGGCATGGTCGCCGGGACGGTGCGGCGCTGGGGCGCCCAACTCGGGCAATTGGCGGCGGTGGTGGC
GATCGTGCTGGCCGGCAAGGCCGCGCTGGCAGAGCCGTTCTACGTGCCGTCCGGCTCGATGGAGCCGACGCTGCTGATCG
GCGACGCGCTGCTCGCCTCGAAATTTCCTTACGGCTACTCGACCGCGTCGCTGCCGATCCACGTGGCGTTTCCCGAGACC
GGCCGGGTGTTCGGCGCCACGCCGCATCGCGGCGACGTCGTGGTGTTTCGCTGGCCCGGCGACCGCTCGCAGGTCTGGGT
CAAGCGCGTGATCGGCCTGCCCGGCGACCGGATCGAACTGACCGGCGGCGTGGTCAGCATCAACGGCGTCGCCGCCACGG
TGAAAGCCGACGGCGTCGGCCGCGCCGAGGACGAAGACGGCGCTTACGAGACCGCCGCGAAATATATCGAGACCCTGCCC
GACGGCGTCTCGCATCCGATCTTCAAGCTGTACGACCATGGCCGGCTCAACAACACGCCGGAGATCATTGTGCCGCCTGG
ACATCTGTTCGTGATGGGCGACAACCGCGACAATTCATCGGACAGCCGCGTGCCGGTGCGCCAGGGCGGCGTCGGGCTGT
TGCCGATGGACAATCTGGTCGGCCGCGTCGACGCCATCGTCGGCTCGTGGAATCCCGGCGTGCGCAAGGAGCCCGTCACC
AACTGGCTGTCCGGCTTCCGCATCGCCCGGTTCTTCACCGCGGTGCATTGA

Upstream 100 bases:

>100_bases
ACGGCATCGGCACCTGCGGCAAGAACGGCCAGGGCGTGCCGGTGGGTGTCGGTCAACCGACGCTGCGGATGGATCGGATC
ACGGTCGGAGGCACCGGCGG

Downstream 100 bases:

>100_bases
GCGATCGGCTCCGAGCACATCGGCAAAGTCCGTCATGCTGAGCGCGCCGCGCTCCACCTCTCGTTCGCCATGGGGCTTTC
CGGGTGATCCTCCGTCTCGT

Product: peptidase S26A, signal peptidase I

Products: NA

Alternate protein names: SPase I; Leader peptidase I [H]

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MNGKAGMVAGTVRRWGAQLGQLAAVVAIVLAGKAALAEPFYVPSGSMEPTLLIGDALLASKFPYGYSTASLPIHVAFPET
GRVFGATPHRGDVVVFRWPGDRSQVWVKRVIGLPGDRIELTGGVVSINGVAATVKADGVGRAEDEDGAYETAAKYIETLP
DGVSHPIFKLYDHGRLNNTPEIIVPPGHLFVMGDNRDNSSDSRVPVRQGGVGLLPMDNLVGRVDAIVGSWNPGVRKEPVT
NWLSGFRIARFFTAVH

Sequences:

>Translated_256_residues
MNGKAGMVAGTVRRWGAQLGQLAAVVAIVLAGKAALAEPFYVPSGSMEPTLLIGDALLASKFPYGYSTASLPIHVAFPET
GRVFGATPHRGDVVVFRWPGDRSQVWVKRVIGLPGDRIELTGGVVSINGVAATVKADGVGRAEDEDGAYETAAKYIETLP
DGVSHPIFKLYDHGRLNNTPEIIVPPGHLFVMGDNRDNSSDSRVPVRQGGVGLLPMDNLVGRVDAIVGSWNPGVRKEPVT
NWLSGFRIARFFTAVH
>Mature_256_residues
MNGKAGMVAGTVRRWGAQLGQLAAVVAIVLAGKAALAEPFYVPSGSMEPTLLIGDALLASKFPYGYSTASLPIHVAFPET
GRVFGATPHRGDVVVFRWPGDRSQVWVKRVIGLPGDRIELTGGVVSINGVAATVKADGVGRAEDEDGAYETAAKYIETLP
DGVSHPIFKLYDHGRLNNTPEIIVPPGHLFVMGDNRDNSSDSRVPVRQGGVGLLPMDNLVGRVDAIVGSWNPGVRKEPVT
NWLSGFRIARFFTAVH

Specific function: Unknown

COG id: COG0681

COG function: function code U; Signal peptidase I

Gene ontology:

Cell location: Cell membrane; Single-pass type II membrane protein (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S26 family [H]

Homologues:

Organism=Escherichia coli, GI1788921, Length=270, Percent_Identity=35.5555555555556, Blast_Score=114, Evalue=5e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000223
- InterPro:   IPR019758
- InterPro:   IPR019757
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056 [H]

Pfam domain/function: PF00717 Peptidase_S24 [H]

EC number: =3.4.21.89 [H]

Molecular weight: Translated: 27279; Mature: 27279

Theoretical pI: Translated: 8.83; Mature: 8.83

Prosite motif: PS00501 SPASE_I_1 ; PS00760 SPASE_I_2 ; PS00761 SPASE_I_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGKAGMVAGTVRRWGAQLGQLAAVVAIVLAGKAALAEPFYVPSGSMEPTLLIGDALLAS
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCEECCCCCCCCEEEECCHHHHH
KFPYGYSTASLPIHVAFPETGRVFGATPHRGDVVVFRWPGDRSQVWVKRVIGLPGDRIEL
CCCCCCCCCCCEEEEECCCCCCEEECCCCCCCEEEEECCCCCHHHHHHHHHCCCCCEEEE
TGGVVSINGVAATVKADGVGRAEDEDGAYETAAKYIETLPDGVSHPIFKLYDHGRLNNTP
ECCEEEECCEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCC
EIIVPPGHLFVMGDNRDNSSDSRVPVRQGGVGLLPMDNLVGRVDAIVGSWNPGVRKEPVT
EEEECCCEEEEEECCCCCCCCCCCCHHCCCEEECCHHHHHHHHHHHHCCCCCCCCCCHHH
NWLSGFRIARFFTAVH
HHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNGKAGMVAGTVRRWGAQLGQLAAVVAIVLAGKAALAEPFYVPSGSMEPTLLIGDALLAS
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCEECCCCCCCCEEEECCHHHHH
KFPYGYSTASLPIHVAFPETGRVFGATPHRGDVVVFRWPGDRSQVWVKRVIGLPGDRIEL
CCCCCCCCCCCEEEEECCCCCCEEECCCCCCCEEEEECCCCCHHHHHHHHHCCCCCEEEE
TGGVVSINGVAATVKADGVGRAEDEDGAYETAAKYIETLPDGVSHPIFKLYDHGRLNNTP
ECCEEEECCEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCC
EIIVPPGHLFVMGDNRDNSSDSRVPVRQGGVGLLPMDNLVGRVDAIVGSWNPGVRKEPVT
EEEECCCEEEEEECCCCCCCCCCCCHHCCCEEECCHHHHHHHHHHHHCCCCCCCCCCHHH
NWLSGFRIARFFTAVH
HHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9823893 [H]