Click here to switch to the map view.

The map label for this gene is shiA [H]

Identifier: 86751664

GI number: 86751664

Start: 5165032

End: 5166696

Strand: Direct

Name: shiA [H]

Synonym: RPB_4566

Alternate gene names: 86751664

Gene position: 5165032-5166696 (Clockwise)

Preceding gene: 86751662

Following gene: 86751668

Centisome position: 96.87

GC content: 64.32

Gene sequence:

>1665_bases
ATGTCTAGTTATGCCGCAACGCAGCGGCGGTCCGGCGGAATGACCAAGGACGAACGTTTCGTCATTGTCGCATCCTCGCT
CGGCACCGTTTTCGAATGGTACGATTTCTATCTGTACGGGTCGCTCGCCGCGATCATCGGCGCGCAGTTCTTCAGCGCCT
ACCCGCCCGCCACACGCGACATTTTCGCCCTTCTGGCGTTCGCCGCCGGCTTCCTGGTGCGCCCGTTCGGCGCCATCGTG
TTCGGCCGCATCGGCGACATCGTCGGCCGCAAATACACCTTCCTCGTCACCATTCTGATCATGGGCCTGTCGACCTTCAT
CGTCGGCCTGCTGCCCAATGCGGCGACCATCGGCATTGCGGCGCCGATCATCCTGATCTGTCTGCGTCTGCTGCAGGGCC
TCGCGCTCGGCGGCGAATATGGCGGCGCGGCGACCTATGTGGCCGAGCATGCTCCACCCGGCAAACGCGGCTACTACACG
TCGTTCATCCAGACCACCGCCACGCTCGGCCTGTTCCTGTCGCTGATCGTGATCCTGGTCACCCGCACGGTGCTGGGCGA
ACCGGAATTCGCCGCCTGGGGTTGGCGCATTCCGTTCCTGGTATCGGTCGCGCTGCTCGGCGTCTCGGTCTGGATCCGGC
TGCGGCTGAACGAATCGCCCGTGTTCCAGAAGATGAAGGAAGAGGGCAAGAGTTCGAAAGCGCCTCTGACCGAAGCCTTC
GCCAATTGGGGCAACGCCAAGATCGTGCTGATCGCCCTGTTCGGCGGCGTGATGGGCCAGGGCGTGGTCTGGTACACCGG
CCAGTTCTACGCGCTGTTCTTCCTGCAATCGATCCTGAAGGTCGACGGCTACACGTCGAACCTGCTGATCGCCTGGTCGC
TGCTGCTCGGCACCTTCTTCTTCATCTTCTTCGGCTGGCTGTCGGACAAGATCGGCCGCAAGCCGATCATCCTGACCGGC
TGCGCGATCGCCGCGCTGTCGTTCTTCCCGATCTTCAAGGCGATCACGACCAACGCCAACCCGGCGCTGGAGCGGGCCAT
CGAGACCGTCAAGGTCGAGGTGGTGTCGGATCCCGCGCTGTGCGGCGACCTGTTCAACCCGGTCGGCACCCGCGTGTTCA
CCGCGCCTTGCGACACTGCGCGCGCCTACCTGTCGCAGTCGTCGGTGAAGTACTCGACCGCGAAGGGCCCGGCGGGCTCG
GGCGTCAAGGTGCTCGTGAACGGCGCCGAGGTGCCCTACGTCGACGCCAAGACCTCCAACCCGGCGGTGCTGGCGGCGAT
CCAGGCGGCGGGCTATCCGAAGGCCGGCAACGCCGAGATCATCAAGATGGCGCATCCGTTCGACGTCTTCCAGCCGCGCA
TCGCGGCGACGATCGGGCTGCTGTTCGTGCTGGTGCTGTTCGTCACCATGGTCTACGGGCCGATCGCCGCGATGCTGGTC
GAACTGTTCCCGACCCGCATCCGCTACACCTCGATGTCGCTGCCCTATCACATCGGCAACGGCTGGTTCGGCGGCCTGCT
GCCGGCGACCGCCTTCGCCATCGTGGCGTCGACCGGCGATATCTACGCCGGCCTCTGGTATCCGATCATCTTCGCGTCGA
TCACCGTGGTGATCGGCCTGATCTTCCTGCCCGAGACCAAGAACGTCGATATCAGCAGGAACTGA

Upstream 100 bases:

>100_bases
GCAATGTTACCGTTCGCCCAACGCACCGCGTCGGAATTCCGCTTTCATTCCGTAACGCGGCAAGAAACGCCATTATTTTA
TCAAGGGGAGGTAATCGCTT

Downstream 100 bases:

>100_bases
CGCTGATCAGTCCGGCGCCGTGCGCGCCGGACTTGTTCTGCCGACGCATTTCGGCCGCGGAGCAATCCGCGGCCGTTTTG
TTTGAAGCAAGCGTCGCGCC

Product: general substrate transporter

Products: Proton [Cytoplasm]; shikimate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 554; Mature: 553

Protein sequence:

>554_residues
MSSYAATQRRSGGMTKDERFVIVASSLGTVFEWYDFYLYGSLAAIIGAQFFSAYPPATRDIFALLAFAAGFLVRPFGAIV
FGRIGDIVGRKYTFLVTILIMGLSTFIVGLLPNAATIGIAAPIILICLRLLQGLALGGEYGGAATYVAEHAPPGKRGYYT
SFIQTTATLGLFLSLIVILVTRTVLGEPEFAAWGWRIPFLVSVALLGVSVWIRLRLNESPVFQKMKEEGKSSKAPLTEAF
ANWGNAKIVLIALFGGVMGQGVVWYTGQFYALFFLQSILKVDGYTSNLLIAWSLLLGTFFFIFFGWLSDKIGRKPIILTG
CAIAALSFFPIFKAITTNANPALERAIETVKVEVVSDPALCGDLFNPVGTRVFTAPCDTARAYLSQSSVKYSTAKGPAGS
GVKVLVNGAEVPYVDAKTSNPAVLAAIQAAGYPKAGNAEIIKMAHPFDVFQPRIAATIGLLFVLVLFVTMVYGPIAAMLV
ELFPTRIRYTSMSLPYHIGNGWFGGLLPATAFAIVASTGDIYAGLWYPIIFASITVVIGLIFLPETKNVDISRN

Sequences:

>Translated_554_residues
MSSYAATQRRSGGMTKDERFVIVASSLGTVFEWYDFYLYGSLAAIIGAQFFSAYPPATRDIFALLAFAAGFLVRPFGAIV
FGRIGDIVGRKYTFLVTILIMGLSTFIVGLLPNAATIGIAAPIILICLRLLQGLALGGEYGGAATYVAEHAPPGKRGYYT
SFIQTTATLGLFLSLIVILVTRTVLGEPEFAAWGWRIPFLVSVALLGVSVWIRLRLNESPVFQKMKEEGKSSKAPLTEAF
ANWGNAKIVLIALFGGVMGQGVVWYTGQFYALFFLQSILKVDGYTSNLLIAWSLLLGTFFFIFFGWLSDKIGRKPIILTG
CAIAALSFFPIFKAITTNANPALERAIETVKVEVVSDPALCGDLFNPVGTRVFTAPCDTARAYLSQSSVKYSTAKGPAGS
GVKVLVNGAEVPYVDAKTSNPAVLAAIQAAGYPKAGNAEIIKMAHPFDVFQPRIAATIGLLFVLVLFVTMVYGPIAAMLV
ELFPTRIRYTSMSLPYHIGNGWFGGLLPATAFAIVASTGDIYAGLWYPIIFASITVVIGLIFLPETKNVDISRN
>Mature_553_residues
SSYAATQRRSGGMTKDERFVIVASSLGTVFEWYDFYLYGSLAAIIGAQFFSAYPPATRDIFALLAFAAGFLVRPFGAIVF
GRIGDIVGRKYTFLVTILIMGLSTFIVGLLPNAATIGIAAPIILICLRLLQGLALGGEYGGAATYVAEHAPPGKRGYYTS
FIQTTATLGLFLSLIVILVTRTVLGEPEFAAWGWRIPFLVSVALLGVSVWIRLRLNESPVFQKMKEEGKSSKAPLTEAFA
NWGNAKIVLIALFGGVMGQGVVWYTGQFYALFFLQSILKVDGYTSNLLIAWSLLLGTFFFIFFGWLSDKIGRKPIILTGC
AIAALSFFPIFKAITTNANPALERAIETVKVEVVSDPALCGDLFNPVGTRVFTAPCDTARAYLSQSSVKYSTAKGPAGSG
VKVLVNGAEVPYVDAKTSNPAVLAAIQAAGYPKAGNAEIIKMAHPFDVFQPRIAATIGLLFVLVLFVTMVYGPIAAMLVE
LFPTRIRYTSMSLPYHIGNGWFGGLLPATAFAIVASTGDIYAGLWYPIIFASITVVIGLIFLPETKNVDISRN

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788292, Length=345, Percent_Identity=34.4927536231884, Blast_Score=193, Evalue=3e-50,
Organism=Escherichia coli, GI1790550, Length=332, Percent_Identity=34.9397590361446, Blast_Score=192, Evalue=6e-50,
Organism=Escherichia coli, GI1789941, Length=329, Percent_Identity=35.8662613981763, Blast_Score=154, Evalue=2e-38,
Organism=Escherichia coli, GI1788942, Length=340, Percent_Identity=31.7647058823529, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI87082404, Length=325, Percent_Identity=24.6153846153846, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI87082231, Length=208, Percent_Identity=26.9230769230769, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 59691; Mature: 59560

Theoretical pI: Translated: 9.59; Mature: 9.59

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSYAATQRRSGGMTKDERFVIVASSLGTVFEWYDFYLYGSLAAIIGAQFFSAYPPATRD
CCCCCCHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
IFALLAFAAGFLVRPFGAIVFGRIGDIVGRKYTFLVTILIMGLSTFIVGLLPNAATIGIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
APIILICLRLLQGLALGGEYGGAATYVAEHAPPGKRGYYTSFIQTTATLGLFLSLIVILV
HHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
TRTVLGEPEFAAWGWRIPFLVSVALLGVSVWIRLRLNESPVFQKMKEEGKSSKAPLTEAF
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHEEEEEEECCCHHHHHHHHHCCCCCCCHHHHH
ANWGNAKIVLIALFGGVMGQGVVWYTGQFYALFFLQSILKVDGYTSNLLIAWSLLLGTFF
HCCCCCEEEEEEEHHHHHHCCEEEECHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
FIFFGWLSDKIGRKPIILTGCAIAALSFFPIFKAITTNANPALERAIETVKVEVVSDPAL
HHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHEEEEECCCHH
CGDLFNPVGTRVFTAPCDTARAYLSQSSVKYSTAKGPAGSGVKVLVNGAEVPYVDAKTSN
HHHHHHHHCCEEEECCCHHHHHHHHHCCCEEECCCCCCCCCCEEEEECCCCCEEECCCCC
PAVLAAIQAAGYPKAGNAEIIKMAHPFDVFQPRIAATIGLLFVLVLFVTMVYGPIAAMLV
CCEEEEEHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELFPTRIRYTSMSLPYHIGNGWFGGLLPATAFAIVASTGDIYAGLWYPIIFASITVVIGL
HHHHHHHEEEEECCCEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
IFLPETKNVDISRN
HCCCCCCCCCCCCC
>Mature Secondary Structure 
SSYAATQRRSGGMTKDERFVIVASSLGTVFEWYDFYLYGSLAAIIGAQFFSAYPPATRD
CCCCCHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
IFALLAFAAGFLVRPFGAIVFGRIGDIVGRKYTFLVTILIMGLSTFIVGLLPNAATIGIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
APIILICLRLLQGLALGGEYGGAATYVAEHAPPGKRGYYTSFIQTTATLGLFLSLIVILV
HHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
TRTVLGEPEFAAWGWRIPFLVSVALLGVSVWIRLRLNESPVFQKMKEEGKSSKAPLTEAF
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHEEEEEEECCCHHHHHHHHHCCCCCCCHHHHH
ANWGNAKIVLIALFGGVMGQGVVWYTGQFYALFFLQSILKVDGYTSNLLIAWSLLLGTFF
HCCCCCEEEEEEEHHHHHHCCEEEECHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
FIFFGWLSDKIGRKPIILTGCAIAALSFFPIFKAITTNANPALERAIETVKVEVVSDPAL
HHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHEEEEECCCHH
CGDLFNPVGTRVFTAPCDTARAYLSQSSVKYSTAKGPAGSGVKVLVNGAEVPYVDAKTSN
HHHHHHHHCCEEEECCCHHHHHHHHHCCCEEECCCCCCCCCCEEEEECCCCCEEECCCCC
PAVLAAIQAAGYPKAGNAEIIKMAHPFDVFQPRIAATIGLLFVLVLFVTMVYGPIAAMLV
CCEEEEEHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELFPTRIRYTSMSLPYHIGNGWFGGLLPATAFAIVASTGDIYAGLWYPIIFASITVVIGL
HHHHHHHEEEEECCCEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
IFLPETKNVDISRN
HCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; shikimate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + shikimate [Periplasm] = Proton [Cytoplasm] + shikimate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9524262; 9097040; 9278503 [H]