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The map label for this gene is braC [H]

Identifier: 86751535

GI number: 86751535

Start: 5024548

End: 5025684

Strand: Direct

Name: braC [H]

Synonym: RPB_4434

Alternate gene names: 86751535

Gene position: 5024548-5025684 (Clockwise)

Preceding gene: 86751534

Following gene: 86751538

Centisome position: 94.24

GC content: 64.56

Gene sequence:

>1137_bases
ATGCTGATGAAGAGATTGATGGTGGCGATCGGGCTTGCGCTCGCTGCGCCGGTGGCGGCCGATGCGCAGAACATCGTGAT
CGGCGCCAGCATCCCCGATACCGGGCCGGCCGCCGCGCCCGCGATATGGCAGCGGTGGGGTTACCAGCTGGCGCTCGACG
AGGCCAATGCGGCGGGCGGCGTGCTCGGCAAGAAAGTCGAGATGCTCGCCTACGACAACCGCTGCAATCCGTCCGAAGCG
GTGAACGTCGCCAACAAGCTGATCGAGGCCAAAGTCGTCGCCATCGTCGGCGCGCATTGTTCGTCGGCGACGCTCGCCAC
GATGCCGTTGATCGCCGCGGCGAAGATTCCGCTGGTGGACGGCATCGCGTCGAGCCCGAAGATCACCGATCTGTCCGGCG
TCGGCGGCAACGAATGGACGTTCCGCATCAACCCCTCCGACGACGACATGATGAACGCGCTCGGCATCTATCTGAGCGGC
AGCTCGAAGATCAAGCGTGTGGCCATATTGGGCGAGGACACCGACTTCGGACGCGGCGGCGCCGCGGCCTTCGCGGCGGT
CGCCAAGAAGCACGGACTCGAGGTGATCTCGACCGACTTCCACCCGCAGAGCTATCCGGACTTCACCGCGCTGCTGACGC
GTATCCAGCAGAGCAAGCCGGACGCGATCGCGATCTTCCAGCTCGCCGGCGATCAGCTCAACTTCCTGCGCAATGCGATG
CAGCTCGGCGTGCGGATTCCGTTCATCGGCCGTTTCGACCCCGGCGGCAACAATCTGCAGATCATCCAGGCCGGCGGCAT
GGAGGGTTCGATCACCGCGTGGACCTACAGCTATCTGGTCGATACGGCCGCCAACAAGGCCTTCGCCGCGGAGATCGAGA
AGCGCCACAAGACAACGCCGGTGCTGCAGACCTGGGCCGGCTACGACGCCATGCGCCTGCTGCTGGCGGCGATCAAGAAC
GCCGGCGCGACCGACCCGACGGCGATCCGCGACGCGATCAAGAAGATCGAGTTCACCAACGTCATGGGGGCCAAAGTCAC
CTTCGACGACCACAACCAGGGTGGCAAGGTCGTGCTGATCGAAGGCGTGGCCGACAAGAAGGTCAAGATCCTGAAGGAAG
TCTCGCTGGCGAACTGA

Upstream 100 bases:

>100_bases
CGCGCCTTCGTGAGCGCCGCCCGGGGCGCGGCGTGAGCACGACGCGAAACGAACATTGCTGTTGCTGTAACCTGAACCCG
TAGTCGAGAGAGGCACGACG

Downstream 100 bases:

>100_bases
CCGGCCCCCGACGCTCGGAGCCCGGCCGGAAAAGTGCCTCGCATTTTTCCGCCGGCCTCCGCCCGCGCAGCAGATCAATC
TGCGGGCCATTCCAGCTTCA

Product: extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: LIVAT-BP; Leu/Ile/Val/Thr/Ala-binding protein [H]

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MLMKRLMVAIGLALAAPVAADAQNIVIGASIPDTGPAAAPAIWQRWGYQLALDEANAAGGVLGKKVEMLAYDNRCNPSEA
VNVANKLIEAKVVAIVGAHCSSATLATMPLIAAAKIPLVDGIASSPKITDLSGVGGNEWTFRINPSDDDMMNALGIYLSG
SSKIKRVAILGEDTDFGRGGAAAFAAVAKKHGLEVISTDFHPQSYPDFTALLTRIQQSKPDAIAIFQLAGDQLNFLRNAM
QLGVRIPFIGRFDPGGNNLQIIQAGGMEGSITAWTYSYLVDTAANKAFAAEIEKRHKTTPVLQTWAGYDAMRLLLAAIKN
AGATDPTAIRDAIKKIEFTNVMGAKVTFDDHNQGGKVVLIEGVADKKVKILKEVSLAN

Sequences:

>Translated_378_residues
MLMKRLMVAIGLALAAPVAADAQNIVIGASIPDTGPAAAPAIWQRWGYQLALDEANAAGGVLGKKVEMLAYDNRCNPSEA
VNVANKLIEAKVVAIVGAHCSSATLATMPLIAAAKIPLVDGIASSPKITDLSGVGGNEWTFRINPSDDDMMNALGIYLSG
SSKIKRVAILGEDTDFGRGGAAAFAAVAKKHGLEVISTDFHPQSYPDFTALLTRIQQSKPDAIAIFQLAGDQLNFLRNAM
QLGVRIPFIGRFDPGGNNLQIIQAGGMEGSITAWTYSYLVDTAANKAFAAEIEKRHKTTPVLQTWAGYDAMRLLLAAIKN
AGATDPTAIRDAIKKIEFTNVMGAKVTFDDHNQGGKVVLIEGVADKKVKILKEVSLAN
>Mature_378_residues
MLMKRLMVAIGLALAAPVAADAQNIVIGASIPDTGPAAAPAIWQRWGYQLALDEANAAGGVLGKKVEMLAYDNRCNPSEA
VNVANKLIEAKVVAIVGAHCSSATLATMPLIAAAKIPLVDGIASSPKITDLSGVGGNEWTFRINPSDDDMMNALGIYLSG
SSKIKRVAILGEDTDFGRGGAAAFAAVAKKHGLEVISTDFHPQSYPDFTALLTRIQQSKPDAIAIFQLAGDQLNFLRNAM
QLGVRIPFIGRFDPGGNNLQIIQAGGMEGSITAWTYSYLVDTAANKAFAAEIEKRHKTTPVLQTWAGYDAMRLLLAAIKN
AGATDPTAIRDAIKKIEFTNVMGAKVTFDDHNQGGKVVLIEGVADKKVKILKEVSLAN

Specific function: Component of the high-affinity leucine, isoleucine, valine transport system I (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI48994941, Length=346, Percent_Identity=25.4335260115607, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI1789867, Length=339, Percent_Identity=25.6637168141593, Blast_Score=97, Evalue=2e-21,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 39984; Mature: 39984

Theoretical pI: Translated: 8.20; Mature: 8.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLMKRLMVAIGLALAAPVAADAQNIVIGASIPDTGPAAAPAIWQRWGYQLALDEANAAGG
CHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCHHHHHHHCCCEEEEECCCCCCC
VLGKKVEMLAYDNRCNPSEAVNVANKLIEAKVVAIVGAHCSSATLATMPLIAAAKIPLVD
CHHCEEEEEEECCCCCHHHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHCCCCCC
GIASSPKITDLSGVGGNEWTFRINPSDDDMMNALGIYLSGSSKIKRVAILGEDTDFGRGG
CCCCCCCCEEECCCCCCEEEEEECCCCHHHHHEEEEEEECCCCEEEEEEEECCCCCCCCH
AAAFAAVAKKHGLEVISTDFHPQSYPDFTALLTRIQQSKPDAIAIFQLAGDQLNFLRNAM
HHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHH
QLGVRIPFIGRFDPGGNNLQIIQAGGMEGSITAWTYSYLVDTAANKAFAAEIEKRHKTTP
HHCEECCEEEEECCCCCEEEEEEECCCCCCEEEEEHHHHHHHHCCHHHHHHHHHHCCCCC
VLQTWAGYDAMRLLLAAIKNAGATDPTAIRDAIKKIEFTNVMGAKVTFDDHNQGGKVVLI
HHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEE
EGVADKKVKILKEVSLAN
ECCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MLMKRLMVAIGLALAAPVAADAQNIVIGASIPDTGPAAAPAIWQRWGYQLALDEANAAGG
CHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCHHHHHHHCCCEEEEECCCCCCC
VLGKKVEMLAYDNRCNPSEAVNVANKLIEAKVVAIVGAHCSSATLATMPLIAAAKIPLVD
CHHCEEEEEEECCCCCHHHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHCCCCCC
GIASSPKITDLSGVGGNEWTFRINPSDDDMMNALGIYLSGSSKIKRVAILGEDTDFGRGG
CCCCCCCCEEECCCCCCEEEEEECCCCHHHHHEEEEEEECCCCEEEEEEEECCCCCCCCH
AAAFAAVAKKHGLEVISTDFHPQSYPDFTALLTRIQQSKPDAIAIFQLAGDQLNFLRNAM
HHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHH
QLGVRIPFIGRFDPGGNNLQIIQAGGMEGSITAWTYSYLVDTAANKAFAAEIEKRHKTTP
HHCEECCEEEEECCCCCEEEEEEECCCCCCEEEEEHHHHHHHHCCHHHHHHHHHHCCCCC
VLQTWAGYDAMRLLLAAIKNAGATDPTAIRDAIKKIEFTNVMGAKVTFDDHNQGGKVVLI
HHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEE
EGVADKKVKILKEVSLAN
ECCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2509433; 2120183; 10984043 [H]