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The map label for this gene is vraA [H]

Identifier: 86751495

GI number: 86751495

Start: 4978618

End: 4979889

Strand: Direct

Name: vraA [H]

Synonym: RPB_4394

Alternate gene names: 86751495

Gene position: 4978618-4979889 (Clockwise)

Preceding gene: 86751494

Following gene: 86751496

Centisome position: 93.38

GC content: 67.69

Gene sequence:

>1272_bases
ATGATCCGTATCGACGACAGACACTACGACGGCGCCCTGCTGCGGGCGATGAGCGAACGCGCGGCCCTCGCCTTGCGGAT
CGCGGATGGCGCTGGCCTCAGCTACGCATTGTGCCTGTCCGACCCCGCTGAATTCCTCACGAGTTTCTTCGCGATCCGCG
ACGCCGGAGCCAGCGTGATCCCGATCCACCCCTCGACGCCGCGCGCCGCGGCGCGTCGGCTGGCGAACAGCGTCGGCTGC
CATCGGCTGATCTGCGATGGCTTCGTCATCGAACACCTCGAAGGCGGCGCTGCCGGTCCCGGACGTTTGCTGCAGACGAC
CTCCGGCACCACCGGCGAGCCGAAGCGGATCGCGCGGAGCTGGGCCGAGATCGATCGCGAGATCGACAGCTACGTCTCGT
CGTTTCGCGAACCGCAGGACATGGCGCCCGTGATCGCCTGCCCGGTCACGCATTCCTACGGGCTGATCTGCGGCCTGCTC
GTGGCGCTCAGGCGCGGACAGGTGCCGCATCTTCTGAGCACCGGCAATCCGAAATTTCTCATCGCCCGCCTGCGCGAAAC
TCACCGGCCGCTGCTGTACTCATCGCCTGCGATGCTGCACACGATCGCAAGGCTGCTGCCGGCCGGCGAGACGATCTTCG
CCGCGATGACCTCGGGCACGCTTCTGCCGGACAGCTGGTTCGCGACGATCCGCGCCCGCACCACGCATTTCTTTCAGCAA
TATGGCTGCTCGGAAGCGGGATGCATCGCGATCAATCCCGATCTGACCGCCGCCGACGAGATGGGGTACCCCCTGCCCCA
TCACAGCCTCGACACCGGCACCATGGACGCCCCGCGCGAAATCGTCCTGCGCGCCGGCGAGCGCGAGATTCGCACCGCCG
ACCTCGGTTACCTGAAAGCCGACGGCCGCCTCGTCTTCGTCGCCAGGCTCGACGATACGATCAACGTCTCCGGCCTCAAC
GTCTATCCGCGAGAGGTCGAGGACGTGGTGATGGCGATGCCCGGCGTCACCGACGCCGTGGCGTTCCGCAAGCTCGACCC
GTTCGCGGGCGAACGGGTGGCCCTCGCCTTCAGCGCCGAACACGTCGTTGCGCCCGCCAGCTTGCGCGAATGGTGCGCGC
AGAACCTCGCCGCCCACCAGGTGCCGATCGAGGCGGTGCGCGTCGCTCAGGTGCCGCGACAGGCAAACGGCAAGATCAAC
CGGCGCGAGATCGCGCGCCTGTATGCCGAGGGCCGGCTCGACGAGCGTCCTGCTGCAGAGGCGACCGCATGA

Upstream 100 bases:

>100_bases
AAAGCCTGCTCGCGCGAAAACTCGGCCTGAATGGCCCCGACTTTCAGCACAAGGTCCCGAATGCGCTTGCCGGCCTTTCG
CTGATGACCCGAGAGCCCGC

Downstream 100 bases:

>100_bases
CCCGCGAAGCGATCATCGCCGCGATCCGCGCCGTGCTCGCGGAGCGGATGGACAACAAACACCTCGACGGCTTCGGCCCG
CAGTCCCGGCTGAACGCCGA

Product: acyl-CoA synthetase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Acyl-CoA synthetase [H]

Number of amino acids: Translated: 423; Mature: 423

Protein sequence:

>423_residues
MIRIDDRHYDGALLRAMSERAALALRIADGAGLSYALCLSDPAEFLTSFFAIRDAGASVIPIHPSTPRAAARRLANSVGC
HRLICDGFVIEHLEGGAAGPGRLLQTTSGTTGEPKRIARSWAEIDREIDSYVSSFREPQDMAPVIACPVTHSYGLICGLL
VALRRGQVPHLLSTGNPKFLIARLRETHRPLLYSSPAMLHTIARLLPAGETIFAAMTSGTLLPDSWFATIRARTTHFFQQ
YGCSEAGCIAINPDLTAADEMGYPLPHHSLDTGTMDAPREIVLRAGEREIRTADLGYLKADGRLVFVARLDDTINVSGLN
VYPREVEDVVMAMPGVTDAVAFRKLDPFAGERVALAFSAEHVVAPASLREWCAQNLAAHQVPIEAVRVAQVPRQANGKIN
RREIARLYAEGRLDERPAAEATA

Sequences:

>Translated_423_residues
MIRIDDRHYDGALLRAMSERAALALRIADGAGLSYALCLSDPAEFLTSFFAIRDAGASVIPIHPSTPRAAARRLANSVGC
HRLICDGFVIEHLEGGAAGPGRLLQTTSGTTGEPKRIARSWAEIDREIDSYVSSFREPQDMAPVIACPVTHSYGLICGLL
VALRRGQVPHLLSTGNPKFLIARLRETHRPLLYSSPAMLHTIARLLPAGETIFAAMTSGTLLPDSWFATIRARTTHFFQQ
YGCSEAGCIAINPDLTAADEMGYPLPHHSLDTGTMDAPREIVLRAGEREIRTADLGYLKADGRLVFVARLDDTINVSGLN
VYPREVEDVVMAMPGVTDAVAFRKLDPFAGERVALAFSAEHVVAPASLREWCAQNLAAHQVPIEAVRVAQVPRQANGKIN
RREIARLYAEGRLDERPAAEATA
>Mature_423_residues
MIRIDDRHYDGALLRAMSERAALALRIADGAGLSYALCLSDPAEFLTSFFAIRDAGASVIPIHPSTPRAAARRLANSVGC
HRLICDGFVIEHLEGGAAGPGRLLQTTSGTTGEPKRIARSWAEIDREIDSYVSSFREPQDMAPVIACPVTHSYGLICGLL
VALRRGQVPHLLSTGNPKFLIARLRETHRPLLYSSPAMLHTIARLLPAGETIFAAMTSGTLLPDSWFATIRARTTHFFQQ
YGCSEAGCIAINPDLTAADEMGYPLPHHSLDTGTMDAPREIVLRAGEREIRTADLGYLKADGRLVFVARLDDTINVSGLN
VYPREVEDVVMAMPGVTDAVAFRKLDPFAGERVALAFSAEHVVAPASLREWCAQNLAAHQVPIEAVRVAQVPRQANGKIN
RREIARLYAEGRLDERPAAEATA

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788107, Length=358, Percent_Identity=24.0223463687151, Blast_Score=72, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI25141359, Length=414, Percent_Identity=21.9806763285024, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6319699, Length=345, Percent_Identity=23.4782608695652, Blast_Score=74, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 45961; Mature: 45961

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRIDDRHYDGALLRAMSERAALALRIADGAGLSYALCLSDPAEFLTSFFAIRDAGASVI
CEEECCCCCCHHHHHHHHHHCEEEEEEECCCCCEEEEECCCHHHHHHHHHHHHCCCCEEE
PIHPSTPRAAARRLANSVGCHRLICDGFVIEHLEGGAAGPGRLLQTTSGTTGEPKRIARS
EECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCHHHHHHH
WAEIDREIDSYVSSFREPQDMAPVIACPVTHSYGLICGLLVALRRGQVPHLLSTGNPKFL
HHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCCCEECCCCCCEE
IARLRETHRPLLYSSPAMLHTIARLLPAGETIFAAMTSGTLLPDSWFATIRARTTHFFQQ
HHHHHHHCCCEEECCCHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHH
YGCSEAGCIAINPDLTAADEMGYPLPHHSLDTGTMDAPREIVLRAGEREIRTADLGYLKA
HCCCCCCEEEECCCCCCHHHCCCCCCCCCCCCCCCCCCHHHHHHCCCCCEEEECCCEEEE
DGRLVFVARLDDTINVSGLNVYPREVEDVVMAMPGVTDAVAFRKLDPFAGERVALAFSAE
CCEEEEEEECCCCEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCCCCEEEEEECCC
HVVAPASLREWCAQNLAAHQVPIEAVRVAQVPRQANGKINRREIARLYAEGRLDERPAAE
CEECCHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHCCCCCHHHHHHHHHCCCCCCCCCCC
ATA
CCC
>Mature Secondary Structure
MIRIDDRHYDGALLRAMSERAALALRIADGAGLSYALCLSDPAEFLTSFFAIRDAGASVI
CEEECCCCCCHHHHHHHHHHCEEEEEEECCCCCEEEEECCCHHHHHHHHHHHHCCCCEEE
PIHPSTPRAAARRLANSVGCHRLICDGFVIEHLEGGAAGPGRLLQTTSGTTGEPKRIARS
EECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCHHHHHHH
WAEIDREIDSYVSSFREPQDMAPVIACPVTHSYGLICGLLVALRRGQVPHLLSTGNPKFL
HHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCCCEECCCCCCEE
IARLRETHRPLLYSSPAMLHTIARLLPAGETIFAAMTSGTLLPDSWFATIRARTTHFFQQ
HHHHHHHCCCEEECCCHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHH
YGCSEAGCIAINPDLTAADEMGYPLPHHSLDTGTMDAPREIVLRAGEREIRTADLGYLKA
HCCCCCCEEEECCCCCCHHHCCCCCCCCCCCCCCCCCCHHHHHHCCCCCEEEECCCEEEE
DGRLVFVARLDDTINVSGLNVYPREVEDVVMAMPGVTDAVAFRKLDPFAGERVALAFSAE
CCEEEEEEECCCCEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCCCCEEEEEECCC
HVVAPASLREWCAQNLAAHQVPIEAVRVAQVPRQANGKINRREIARLYAEGRLDERPAAE
CEECCHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHCCCCCHHHHHHHHHCCCCCCCCCCC
ATA
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0024 {myristate}} 0.0021 {myristate}} 0.0012 {myristate}} 0.0002 {myristate}} 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0112 {hexanoate}} 0.0083 {decanoate}} 0.0063 {decanoate}} 0.0043 {dec

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA