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The map label for this gene is fecI [H]

Identifier: 86751492

GI number: 86751492

Start: 4974322

End: 4974822

Strand: Reverse

Name: fecI [H]

Synonym: RPB_4391

Alternate gene names: 86751492

Gene position: 4974822-4974322 (Counterclockwise)

Preceding gene: 86751506

Following gene: 86751489

Centisome position: 93.31

GC content: 64.07

Gene sequence:

>501_bases
ATGTCGTCTGCGACAGAACTGCTCAATATCTTCGGAACGGAGCAAGGCCACCTCCAGCGGCTGGTCCGCCGCATCACAGG
CGATGCCCATACCGCCGAGGACGTCGTCCACGAGACCTTCATCAAGCTGATGAACCGAGATGTCGGCCAGCGCGACGTCG
GTCTTGTCGTGACGACTGCGCGAAATCTCGCGCGCGACGCCATGCGGGCGGAACGCGTGCGTACCTCCTATGCGGAGAGC
GTGTCGCGTGAGCAGCTTCAGCCCGGCGTCGTCGGTCCCGAGGATGCGGTTGCGAGCCGGATGGATCTCGACGCGCTGTT
GCGCTCGCTGCAGACGCTGCCTCGACGCACCCAGCGCATCTTTCTTTTGAGCAGGGTCGACGGCATGACCTATCCGCAGA
TCGCCCGCAAACTGGGTGTCTCGGTCTCGACCGTCGAGAAGGAGATGATCTCCGCCCTCGAATTCTGCCGCAACTGGCGG
CGCCGGCGCGAGACCCTCTGA

Upstream 100 bases:

>100_bases
CATCGCTTTTGAATCGTTAAAAATAGTGATGCGGCAGCCTTAATATTGTCTCCGGACGCTTGCTGTTCGGATCGGGCTGA
TGCGCGACGATGAGGGTACC

Downstream 100 bases:

>100_bases
GCCCCTGCCGTATTCGGACGGTGAATAGGATTTGCCCAAGGCCTGGTTCGGAACACCGTGCCTGTGAGCGCCGCGGCCTG
CGAAACTACGCGGAGAGGAA

Product: sigma-24 (FecI-like)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 166; Mature: 165

Protein sequence:

>166_residues
MSSATELLNIFGTEQGHLQRLVRRITGDAHTAEDVVHETFIKLMNRDVGQRDVGLVVTTARNLARDAMRAERVRTSYAES
VSREQLQPGVVGPEDAVASRMDLDALLRSLQTLPRRTQRIFLLSRVDGMTYPQIARKLGVSVSTVEKEMISALEFCRNWR
RRRETL

Sequences:

>Translated_166_residues
MSSATELLNIFGTEQGHLQRLVRRITGDAHTAEDVVHETFIKLMNRDVGQRDVGLVVTTARNLARDAMRAERVRTSYAES
VSREQLQPGVVGPEDAVASRMDLDALLRSLQTLPRRTQRIFLLSRVDGMTYPQIARKLGVSVSTVEKEMISALEFCRNWR
RRRETL
>Mature_165_residues
SSATELLNIFGTEQGHLQRLVRRITGDAHTAEDVVHETFIKLMNRDVGQRDVGLVVTTARNLARDAMRAERVRTSYAESV
SREQLQPGVVGPEDAVASRMDLDALLRSLQTLPRRTQRIFLLSRVDGMTYPQIARKLGVSVSTVEKEMISALEFCRNWRR
RRETL

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor regulates the fec genes for iron dicitrate transport (Probable) [H]

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790746, Length=157, Percent_Identity=32.484076433121, Blast_Score=76, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 18865; Mature: 18734

Theoretical pI: Translated: 10.62; Mature: 10.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSATELLNIFGTEQGHLQRLVRRITGDAHTAEDVVHETFIKLMNRDVGQRDVGLVVTTA
CCHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
RNLARDAMRAERVRTSYAESVSREQLQPGVVGPEDAVASRMDLDALLRSLQTLPRRTQRI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLLSRVDGMTYPQIARKLGVSVSTVEKEMISALEFCRNWRRRRETL
HHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SSATELLNIFGTEQGHLQRLVRRITGDAHTAEDVVHETFIKLMNRDVGQRDVGLVVTTA
CHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
RNLARDAMRAERVRTSYAESVSREQLQPGVVGPEDAVASRMDLDALLRSLQTLPRRTQRI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLLSRVDGMTYPQIARKLGVSVSTVEKEMISALEFCRNWRRRRETL
HHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2254251; 7610040; 9278503; 8052622 [H]