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The map label for this gene is rfaG [H]

Identifier: 86751343

GI number: 86751343

Start: 4812559

End: 4813644

Strand: Reverse

Name: rfaG [H]

Synonym: RPB_4237

Alternate gene names: 86751343

Gene position: 4813644-4812559 (Counterclockwise)

Preceding gene: 86751344

Following gene: 86751342

Centisome position: 90.28

GC content: 70.81

Gene sequence:

>1086_bases
ATGAAGGTCATGCTGGCGATCTTCAAACTCGACAGACTGGGCGGCAAGGAACGCGATTGCATGGCGATCGCGCGGCACCT
TGCGGCGCGTGGTCACGACGTCACCGTGCTGACGACGTCGGCCGACGTCGCGGCCATCGACGATCTGCGGATCGAGAGCC
TGCGCGCCCGCGGGCTCGCCAATCACGTGCTGCTGCGCAATTTTGCGCGCGACGTGATCGACCGCAGGCAGCGCGAGCGG
CCCGATGCGCTGCTGTCGTTCGAGCGAATCCCCGACGCCGATTATCACTACGTCGCCGACGGCGCCGCGATCCTGCGCGC
CTGGCAGCTGCTGGCGTGGCCGCCGCGCCGCCGCGCCAAGCTGGCGCTGGAGCGCGCGGTGTTCGCGGCGCCGGCCGCGA
CCCGGTTGTTCTTCCTCACCGAGCGCCAGCGCGACGAATACATCATCGCCTATGATTTCGAGCCGGCCCGCGCCAGCGTG
CTGCCGATGGTGCTGCACGACGACCGCTACGCCGCCGCGCGCAAGCTCGGCGCCTCCCGCTGGCGCAGCGAGCTCGGCAT
TCCCGGCGACGCGCTGATGGCGGTGTCGGTCGCGGTCGATCCGAAGCTCAAGGGCGTCGATCGTAGCCTCGCCGCGCTGG
CGTCCTATCCGAAGCTTCACCTCGTCGTCGCCGGTTCGGATTCGCCGTGGCTGCATCGCGGCGTGGTGCGGCGCGATCTC
GAGCGGCGCGTGCACATCGTGCCTTACGTCGCCGAGGTGATGGAGCTGATCGCGGCGGCCGATTTCATGCTGCACCCCGC
GCGCTCCGAAGCGGCCGGGCAAGTGATCGGCGAGGCGCTGCTCGCCGGCGTGCCGGTGCTCGCCTCGGCCGCCTGCGGCT
ATGCCGGCGAGATCGAGCGCAGCGGCGCCGGCCTGGTGCTGCCGGAGCCGTTCCAGCAGGAGGCGCTGGTCGCCGGCATC
GCCGCGATGATCGACGCACTGCCGGCGATGCGCAAGCAAGCGGCGGCGCGCGCGAAGAGCCTGCAGCAGCAGCGCGGCGC
GTGGCTGTTGGCGATCGCCGAACGGATCGAACAGCGCGACGTCTGA

Upstream 100 bases:

>100_bases
TGTTTCTCATGCATCACCGCGTTGCACCATCGCGTTCCGCCGCGCTATAACCGCGCCGTTGTGATGATCCGGATGCACCT
TCGATGAGCCGGCGTTTCCC

Downstream 100 bases:

>100_bases
ATCCGGGTGGATCACGGCGCCCGCCGCGGCTAGACTCGCCGGCCCATCAGCAACGAGAATTCCGATGACGGCGTACAACT
ACGCGATGTTCGACACCGCG

Product: glycosyl transferase, group 1

Products: NA

Alternate protein names: Glucosyltransferase I [H]

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MKVMLAIFKLDRLGGKERDCMAIARHLAARGHDVTVLTTSADVAAIDDLRIESLRARGLANHVLLRNFARDVIDRRQRER
PDALLSFERIPDADYHYVADGAAILRAWQLLAWPPRRRAKLALERAVFAAPAATRLFFLTERQRDEYIIAYDFEPARASV
LPMVLHDDRYAAARKLGASRWRSELGIPGDALMAVSVAVDPKLKGVDRSLAALASYPKLHLVVAGSDSPWLHRGVVRRDL
ERRVHIVPYVAEVMELIAAADFMLHPARSEAAGQVIGEALLAGVPVLASAACGYAGEIERSGAGLVLPEPFQQEALVAGI
AAMIDALPAMRKQAAARAKSLQQQRGAWLLAIAERIEQRDV

Sequences:

>Translated_361_residues
MKVMLAIFKLDRLGGKERDCMAIARHLAARGHDVTVLTTSADVAAIDDLRIESLRARGLANHVLLRNFARDVIDRRQRER
PDALLSFERIPDADYHYVADGAAILRAWQLLAWPPRRRAKLALERAVFAAPAATRLFFLTERQRDEYIIAYDFEPARASV
LPMVLHDDRYAAARKLGASRWRSELGIPGDALMAVSVAVDPKLKGVDRSLAALASYPKLHLVVAGSDSPWLHRGVVRRDL
ERRVHIVPYVAEVMELIAAADFMLHPARSEAAGQVIGEALLAGVPVLASAACGYAGEIERSGAGLVLPEPFQQEALVAGI
AAMIDALPAMRKQAAARAKSLQQQRGAWLLAIAERIEQRDV
>Mature_361_residues
MKVMLAIFKLDRLGGKERDCMAIARHLAARGHDVTVLTTSADVAAIDDLRIESLRARGLANHVLLRNFARDVIDRRQRER
PDALLSFERIPDADYHYVADGAAILRAWQLLAWPPRRRAKLALERAVFAAPAATRLFFLTERQRDEYIIAYDFEPARASV
LPMVLHDDRYAAARKLGASRWRSELGIPGDALMAVSVAVDPKLKGVDRSLAALASYPKLHLVVAGSDSPWLHRGVVRRDL
ERRVHIVPYVAEVMELIAAADFMLHPARSEAAGQVIGEALLAGVPVLASAACGYAGEIERSGAGLVLPEPFQQEALVAGI
AAMIDALPAMRKQAAARAKSLQQQRGAWLLAIAERIEQRDV

Specific function: Involved in the addition of the first glucose residue to the lipopolysaccharide core [H]

COG id: COG0438

COG function: function code M; Glycosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790061, Length=328, Percent_Identity=34.1463414634146, Blast_Score=160, Evalue=2e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296 [H]

Pfam domain/function: PF00534 Glycos_transf_1 [H]

EC number: NA

Molecular weight: Translated: 39686; Mature: 39686

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVMLAIFKLDRLGGKERDCMAIARHLAARGHDVTVLTTSADVAAIDDLRIESLRARGLA
CHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHH
NHVLLRNFARDVIDRRQRERPDALLSFERIPDADYHYVADGAAILRAWQLLAWPPRRRAK
HHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHCCCCCHHHH
LALERAVFAAPAATRLFFLTERQRDEYIIAYDFEPARASVLPMVLHDDRYAAARKLGASR
HHHHHHHHHCCHHHHEEEEEECCCCCEEEEEECCCCCHHEEEEEEECCHHHHHHHHHHHH
WRSELGIPGDALMAVSVAVDPKLKGVDRSLAALASYPKLHLVVAGSDSPWLHRGVVRRDL
HHHHCCCCCHHHEEEEEEECCCCCCCHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHH
ERRVHIVPYVAEVMELIAAADFMLHPARSEAAGQVIGEALLAGVPVLASAACGYAGEIER
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCC
SGAGLVLPEPFQQEALVAGIAAMIDALPAMRKQAAARAKSLQQQRGAWLLAIAERIEQRD
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCC
V
C
>Mature Secondary Structure
MKVMLAIFKLDRLGGKERDCMAIARHLAARGHDVTVLTTSADVAAIDDLRIESLRARGLA
CHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHH
NHVLLRNFARDVIDRRQRERPDALLSFERIPDADYHYVADGAAILRAWQLLAWPPRRRAK
HHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHCCCCCHHHH
LALERAVFAAPAATRLFFLTERQRDEYIIAYDFEPARASVLPMVLHDDRYAAARKLGASR
HHHHHHHHHCCHHHHEEEEEECCCCCEEEEEECCCCCHHEEEEEEECCHHHHHHHHHHHH
WRSELGIPGDALMAVSVAVDPKLKGVDRSLAALASYPKLHLVVAGSDSPWLHRGVVRRDL
HHHHCCCCCHHHEEEEEEECCCCCCCHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHH
ERRVHIVPYVAEVMELIAAADFMLHPARSEAAGQVIGEALLAGVPVLASAACGYAGEIER
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCC
SGAGLVLPEPFQQEALVAGIAAMIDALPAMRKQAAARAKSLQQQRGAWLLAIAERIEQRD
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCC
V
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Glycosyltransferases; Hexosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1732225; 8041620; 9278503; 1447141; 7988890 [H]