Click here to switch to the map view.

The map label for this gene is ogt [H]

Identifier: 86751342

GI number: 86751342

Start: 4811952

End: 4812494

Strand: Reverse

Name: ogt [H]

Synonym: RPB_4236

Alternate gene names: 86751342

Gene position: 4812494-4811952 (Counterclockwise)

Preceding gene: 86751343

Following gene: 86751338

Centisome position: 90.26

GC content: 69.8

Gene sequence:

>543_bases
ATGACGGCGTACAACTACGCGATGTTCGACACCGCGCTCGGCCGCTGCGGCATCGCCTGGGGCGATCGCGGCGTCGTCGC
CGTGCAACTGCCGCAGCCGAGCGAGGCGCAGACCCGCGCGCGGATCGCCCAACGCCAGGGCGCGATCGACGAAGCCGCGC
CGCCGGCGCCGGTGCAAGACGCGATCGACGGCATCGTCGCACTGCTCGCAGGCCAGCCGATCGATCTCACGCATATTGCG
CTCGACCTCGACGGCGTCTCCGAATTCCAGCGCGGCGTCTACGCCATCGCGCGCACCATCCCGCCGGGGCACACGCTGAG
TTACGGCGACATCGCCAAACGACTCGGCGGAGTCGAATTGGCGCGCGAGGTCGGCCAGGCGCTCGGTCGCAATCCGGTGC
CGGTGATCGTGCCGTGCCACCGCGTGCTCGCCGCCGGCGGCAAGCCCGGCGGATTTTCCGCCAATGGCGGCGTCGCCACC
AAGCTCAAACTGCTGTCGATCGAGGGCGCGGCGGTCAACTACACGCCGAGCCTGTTCGATTGA

Upstream 100 bases:

>100_bases
GCGATCGCCGAACGGATCGAACAGCGCGACGTCTGAATCCGGGTGGATCACGGCGCCCGCCGCGGCTAGACTCGCCGGCC
CATCAGCAACGAGAATTCCG

Downstream 100 bases:

>100_bases
ACGGCGGAATTCTTGCGGCGCGACCTGCCTGAAGTTTGTCACAGGTTTGCCGCAAGCCACCAAGACTCACGCCGGCGGCG
CCAAGGCCCGCGCCGGCGGC

Product: methylated-DNA--protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 180; Mature: 179

Protein sequence:

>180_residues
MTAYNYAMFDTALGRCGIAWGDRGVVAVQLPQPSEAQTRARIAQRQGAIDEAAPPAPVQDAIDGIVALLAGQPIDLTHIA
LDLDGVSEFQRGVYAIARTIPPGHTLSYGDIAKRLGGVELAREVGQALGRNPVPVIVPCHRVLAAGGKPGGFSANGGVAT
KLKLLSIEGAAVNYTPSLFD

Sequences:

>Translated_180_residues
MTAYNYAMFDTALGRCGIAWGDRGVVAVQLPQPSEAQTRARIAQRQGAIDEAAPPAPVQDAIDGIVALLAGQPIDLTHIA
LDLDGVSEFQRGVYAIARTIPPGHTLSYGDIAKRLGGVELAREVGQALGRNPVPVIVPCHRVLAAGGKPGGFSANGGVAT
KLKLLSIEGAAVNYTPSLFD
>Mature_179_residues
TAYNYAMFDTALGRCGIAWGDRGVVAVQLPQPSEAQTRARIAQRQGAIDEAAPPAPVQDAIDGIVALLAGQPIDLTHIAL
DLDGVSEFQRGVYAIARTIPPGHTLSYGDIAKRLGGVELAREVGQALGRNPVPVIVPCHRVLAAGGKPGGFSANGGVATK
LKLLSIEGAAVNYTPSLFD

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Escherichia coli, GI1788542, Length=167, Percent_Identity=34.1317365269461, Blast_Score=83, Evalue=8e-18,
Organism=Escherichia coli, GI1787596, Length=85, Percent_Identity=45.8823529411765, Blast_Score=71, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI115533068, Length=92, Percent_Identity=39.1304347826087, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI115533070, Length=92, Percent_Identity=39.1304347826087, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6320001, Length=76, Percent_Identity=38.1578947368421, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1 [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 18679; Mature: 18547

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAYNYAMFDTALGRCGIAWGDRGVVAVQLPQPSEAQTRARIAQRQGAIDEAAPPAPVQD
CCCCHHHHHHHHHHHCCCEECCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCHHH
AIDGIVALLAGQPIDLTHIALDLDGVSEFQRGVYAIARTIPPGHTLSYGDIAKRLGGVEL
HHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCHHHH
AREVGQALGRNPVPVIVPCHRVLAAGGKPGGFSANGGVATKLKLLSIEGAAVNYTPSLFD
HHHHHHHHCCCCCEEEECCHHHHHCCCCCCCCCCCCCCEEEEEEEEECCCEEECCCCCCC
>Mature Secondary Structure 
TAYNYAMFDTALGRCGIAWGDRGVVAVQLPQPSEAQTRARIAQRQGAIDEAAPPAPVQD
CCCHHHHHHHHHHHCCCEECCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCHHH
AIDGIVALLAGQPIDLTHIALDLDGVSEFQRGVYAIARTIPPGHTLSYGDIAKRLGGVEL
HHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCHHHH
AREVGQALGRNPVPVIVPCHRVLAAGGKPGGFSANGGVATKLKLLSIEGAAVNYTPSLFD
HHHHHHHHCCCCCEEEECCHHHHHCCCCCCCCCCCCCCEEEEEEEEECCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]