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The map label for this gene is nemA [H]

Identifier: 86751316

GI number: 86751316

Start: 4782242

End: 4783360

Strand: Direct

Name: nemA [H]

Synonym: RPB_4210

Alternate gene names: 86751316

Gene position: 4782242-4783360 (Clockwise)

Preceding gene: 86751315

Following gene: 86751331

Centisome position: 89.7

GC content: 64.34

Gene sequence:

>1119_bases
ATGTTCCGGAACGGCAAGATGACAAATTCCAAACTGTTCGAACCCTATCAGCTCGGCCCGATCACGCTGTCCAACCGCAT
CGCGATGGCGCCGCTCACCCGCAATCGCGCGATTCCCGATGGGCTCGTCCCCAGTCCGCTGGCGGCGGAATATTACGGCC
AGCGCGCCAGCGCCGGGCTGTTGATCACCGAAGCGAGTCAGGTCTCGCAGCAGGGCCAGGGCTATCAGGACACACCCGGC
ATCTATTCCGACGCGCAGGTCACAGCCTGGAAGAAGGTCACCGACGAGGTGCACAAGCGCGGCGGCAAGATCTTCATTCA
ACTCTGGCACGTCGGCCGCATCTCGCATGTCGATCTGCAGCCGAACCACGGCGCCCCGGTGGCGCCGTCAGCCATCAAGG
CCGCCACCAAAACCTTCGTCGGTGGCAAATTCGCCGATGTCTCCGAGCCGCGCGCGCTCGAGCTCGGCGAAATTCCCGGC
ATCATCGACGACTTCCGCCAGGGCGCGGCCAATGCGATCAAGGCCGGCTTCGACGGCGTCGAGATCCACGGCGCCAACGG
CTATCTGCTCGACCAGTTCGCCCGCGACAGCAGCAACAAGCGCACCGATTCTTATGGCGGATCGATCGAGAACCGCGCCA
GGCTGATGCTCGAAGTAGCGGCGGCCGTCGCCAAGGAGATCGGGCCGGAGCGCACCGGCATCCGGATTTCGCCGGTGACG
CCGGCCAATGATGTGTCGGACTCCAATCCGCAGGCATTGTTCGACTACATCGTCGACGAACTCGACAAGCTGAAGCTGGT
CTATATCCACGTCATCGAGGGCGCGACCGGCGGCCCGCGCGACGTCGCGCCGTTCGACTACGCGTCCCTGCGCAAGCGCT
TCAGCGGCGCCTACATCGGCAACAATGCCTATGATCTTACGCTCGCCGACAAGCAGCTCGACGCCAATGCGGCCGACCTG
ATCGCCTTCGGCAAGCCGTTCATCTCCAACCCCGACCTCGTCGAACGCCTGAAGAGCGGCGCGCCGCTCAACGAGCCGGA
CAAGTCGACGTTCTACGGCGGCGGCGCCAAGGGCTACACCGACTACCCGACGCTGGAATCGGCGCAAGCGGCGCAGTAA

Upstream 100 bases:

>100_bases
CCCAGTTTCGGAATTGCCCTGCAAATTTCATGCAGCCGCATCTATTTTGTGCGGTGCCATTGTGTTTGCCCCGGAACGCC
CCACGTTCTTTGCATCCGCA

Downstream 100 bases:

>100_bases
GCCACCCCCACCGTCATTCCGGGGCGCGAGCGCAGTTCGCGAACCCGGAATCCAGAAATTGGTGCGGAGAGATTCCGGGT
TCGCTCGCTTCGCGAGCGTC

Product: NADH:flavin oxidoreductase/NADH oxidase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MFRNGKMTNSKLFEPYQLGPITLSNRIAMAPLTRNRAIPDGLVPSPLAAEYYGQRASAGLLITEASQVSQQGQGYQDTPG
IYSDAQVTAWKKVTDEVHKRGGKIFIQLWHVGRISHVDLQPNHGAPVAPSAIKAATKTFVGGKFADVSEPRALELGEIPG
IIDDFRQGAANAIKAGFDGVEIHGANGYLLDQFARDSSNKRTDSYGGSIENRARLMLEVAAAVAKEIGPERTGIRISPVT
PANDVSDSNPQALFDYIVDELDKLKLVYIHVIEGATGGPRDVAPFDYASLRKRFSGAYIGNNAYDLTLADKQLDANAADL
IAFGKPFISNPDLVERLKSGAPLNEPDKSTFYGGGAKGYTDYPTLESAQAAQ

Sequences:

>Translated_372_residues
MFRNGKMTNSKLFEPYQLGPITLSNRIAMAPLTRNRAIPDGLVPSPLAAEYYGQRASAGLLITEASQVSQQGQGYQDTPG
IYSDAQVTAWKKVTDEVHKRGGKIFIQLWHVGRISHVDLQPNHGAPVAPSAIKAATKTFVGGKFADVSEPRALELGEIPG
IIDDFRQGAANAIKAGFDGVEIHGANGYLLDQFARDSSNKRTDSYGGSIENRARLMLEVAAAVAKEIGPERTGIRISPVT
PANDVSDSNPQALFDYIVDELDKLKLVYIHVIEGATGGPRDVAPFDYASLRKRFSGAYIGNNAYDLTLADKQLDANAADL
IAFGKPFISNPDLVERLKSGAPLNEPDKSTFYGGGAKGYTDYPTLESAQAAQ
>Mature_372_residues
MFRNGKMTNSKLFEPYQLGPITLSNRIAMAPLTRNRAIPDGLVPSPLAAEYYGQRASAGLLITEASQVSQQGQGYQDTPG
IYSDAQVTAWKKVTDEVHKRGGKIFIQLWHVGRISHVDLQPNHGAPVAPSAIKAATKTFVGGKFADVSEPRALELGEIPG
IIDDFRQGAANAIKAGFDGVEIHGANGYLLDQFARDSSNKRTDSYGGSIENRARLMLEVAAAVAKEIGPERTGIRISPVT
PANDVSDSNPQALFDYIVDELDKLKLVYIHVIEGATGGPRDVAPFDYASLRKRFSGAYIGNNAYDLTLADKQLDANAADL
IAFGKPFISNPDLVERLKSGAPLNEPDKSTFYGGGAKGYTDYPTLESAQAAQ

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=365, Percent_Identity=50.958904109589, Blast_Score=354, Evalue=7e-99,
Organism=Escherichia coli, GI1789463, Length=347, Percent_Identity=29.6829971181556, Blast_Score=128, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI17559802, Length=286, Percent_Identity=30.4195804195804, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17559804, Length=277, Percent_Identity=28.5198555956679, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17564188, Length=278, Percent_Identity=26.978417266187, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17565138, Length=366, Percent_Identity=27.3224043715847, Blast_Score=92, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17540738, Length=263, Percent_Identity=29.6577946768061, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI72001454, Length=275, Percent_Identity=28, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17566914, Length=261, Percent_Identity=26.8199233716475, Blast_Score=72, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6325086, Length=382, Percent_Identity=40.0523560209424, Blast_Score=244, Evalue=2e-65,
Organism=Saccharomyces cerevisiae, GI6321973, Length=392, Percent_Identity=37.2448979591837, Blast_Score=227, Evalue=2e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 40028; Mature: 40028

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRNGKMTNSKLFEPYQLGPITLSNRIAMAPLTRNRAIPDGLVPSPLAAEYYGQRASAGL
CCCCCCCCCCCCCCCCCCCCEEECCCEEECCCCCCCCCCCCCCCCCHHHHHHCCCCCCCE
LITEASQVSQQGQGYQDTPGIYSDAQVTAWKKVTDEVHKRGGKIFIQLWHVGRISHVDLQ
EEECHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEECCCEEEEEEC
PNHGAPVAPSAIKAATKTFVGGKFADVSEPRALELGEIPGIIDDFRQGAANAIKAGFDGV
CCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHCCCCCE
EIHGANGYLLDQFARDSSNKRTDSYGGSIENRARLMLEVAAAVAKEIGPERTGIRISPVT
EEECCCCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCC
PANDVSDSNPQALFDYIVDELDKLKLVYIHVIEGATGGPRDVAPFDYASLRKRFSGAYIG
CCCCCCCCCHHHHHHHHHHHHHCEEEEEEEEEECCCCCCCCCCCCCHHHHHHHHCCEEEC
NNAYDLTLADKQLDANAADLIAFGKPFISNPDLVERLKSGAPLNEPDKSTFYGGGAKGYT
CCEEEEEEECCCCCCCHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCCCCCEECCCCCCCC
DYPTLESAQAAQ
CCCCCCCCCCCC
>Mature Secondary Structure
MFRNGKMTNSKLFEPYQLGPITLSNRIAMAPLTRNRAIPDGLVPSPLAAEYYGQRASAGL
CCCCCCCCCCCCCCCCCCCCEEECCCEEECCCCCCCCCCCCCCCCCHHHHHHCCCCCCCE
LITEASQVSQQGQGYQDTPGIYSDAQVTAWKKVTDEVHKRGGKIFIQLWHVGRISHVDLQ
EEECHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEECCCEEEEEEC
PNHGAPVAPSAIKAATKTFVGGKFADVSEPRALELGEIPGIIDDFRQGAANAIKAGFDGV
CCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHCCCCCE
EIHGANGYLLDQFARDSSNKRTDSYGGSIENRARLMLEVAAAVAKEIGPERTGIRISPVT
EEECCCCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCC
PANDVSDSNPQALFDYIVDELDKLKLVYIHVIEGATGGPRDVAPFDYASLRKRFSGAYIG
CCCCCCCCCHHHHHHHHHHHHHCEEEEEEEEEECCCCCCCCCCCCCHHHHHHHHCCEEEC
NNAYDLTLADKQLDANAADLIAFGKPFISNPDLVERLKSGAPLNEPDKSTFYGGGAKGYT
CCEEEEEEECCCCCCCHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCCCCCEECCCCCCCC
DYPTLESAQAAQ
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]