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The map label for this gene is prs [H]

Identifier: 86751275

GI number: 86751275

Start: 4739357

End: 4740310

Strand: Direct

Name: prs [H]

Synonym: RPB_4168

Alternate gene names: 86751275

Gene position: 4739357-4740310 (Clockwise)

Preceding gene: 86751274

Following gene: 86751277

Centisome position: 88.89

GC content: 64.57

Gene sequence:

>954_bases
ATGTCGTGGAAAAACGGCTCCGTCAAGCTTGTCGCCGGCAATTCCAATCCCGAGCTGGCGCGCGGCATTGCCGACTGGCT
GAAGATGCCGCTCACCAAAGCCAGCGTCCGCCGCTTCGCCGACAACGAGGTGTTCGTCGAGATCCAGGAGAACGTCCGCG
GCTCGGATGTCTACATCATCCAGTCGACGTCGTTTCCGACCAACGACCATCTGATGGAACTCTTGATCATCACCGACGCG
CTGCGCCGCGCCTCGGCGCGCCGGATCACCGCGGTGATCCCGTATTTCGGCTACGCCCGCCAGGACCGCAAGGCCGGTGG
ACGAACGCCGATCTCGGCCAAGCTGGTCGCCAATCTGATCACCCATGCGGGGACGGATCGGGTGATGACGCTCGATCTGC
ACGCCGGCCAGATCCAGGGCTTCTTCGATATCCCGACCGATAACCTGTTCGCCTCGCCGGTGATGGTGCGCGACATCAAG
GAGCGCTTCGACCTGTCGAAAGTCGCAGTGGTGTCGCCCGACGTCGGCGGCGTGGTCCGCGCCCGCGGTCTCGCCAAGCG
CATCAACGCGCCGCTGGCGATCATCGACAAGCGCCGCGAGCGCGCCGGCGAATCCGAAGTGATGAACGTGATCGGCGAAG
TCGAGGGCTATACCTGCATCCTGATCGACGACATCGTCGATTCCGGCGGCACGCTGGTGAACGCCGCCGACGCGCTGCTC
GCCAACGGCGCCAAGGACGTCTACGCCTACATCACCCACGGCGTGCTGTCCGGCGGCGCCTGCGCCCGCATCAATTCCTC
GAAGCTGAAAGAGCTGGTGATCACCGACTCGATCCAGCCGACCAACGCGGTGTGCAACAGCGCCAACATCCGCACCCTGT
CGATCGCCTCGCTGATCGCCGAAGCGATCGGCCGCACCGCCTCCGAAGAGTCGGTGTCGAGCCTGTTCGACTGA

Upstream 100 bases:

>100_bases
CGCCACCGGCTGATATCACCTCGCTCGCAAAAGCGCCCGTTTTCGTGAGTCAACCGATATGCTGAACGTTGTGTCCACCG
CGGCGAGGGGAAACGCGTCG

Downstream 100 bases:

>100_bases
TAACTCACCCCCACACCGTCATTCCGGGGCACGCGCGCAGCGCGTGAACCCGGAATCCAGACGTTCAGGGCACCTCTGGA
TTCCGGGTTCGCGAGCTGCG

Product: ribose-phosphate pyrophosphokinase

Products: NA

Alternate protein names: RPPK; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase [H]

Number of amino acids: Translated: 317; Mature: 316

Protein sequence:

>317_residues
MSWKNGSVKLVAGNSNPELARGIADWLKMPLTKASVRRFADNEVFVEIQENVRGSDVYIIQSTSFPTNDHLMELLIITDA
LRRASARRITAVIPYFGYARQDRKAGGRTPISAKLVANLITHAGTDRVMTLDLHAGQIQGFFDIPTDNLFASPVMVRDIK
ERFDLSKVAVVSPDVGGVVRARGLAKRINAPLAIIDKRRERAGESEVMNVIGEVEGYTCILIDDIVDSGGTLVNAADALL
ANGAKDVYAYITHGVLSGGACARINSSKLKELVITDSIQPTNAVCNSANIRTLSIASLIAEAIGRTASEESVSSLFD

Sequences:

>Translated_317_residues
MSWKNGSVKLVAGNSNPELARGIADWLKMPLTKASVRRFADNEVFVEIQENVRGSDVYIIQSTSFPTNDHLMELLIITDA
LRRASARRITAVIPYFGYARQDRKAGGRTPISAKLVANLITHAGTDRVMTLDLHAGQIQGFFDIPTDNLFASPVMVRDIK
ERFDLSKVAVVSPDVGGVVRARGLAKRINAPLAIIDKRRERAGESEVMNVIGEVEGYTCILIDDIVDSGGTLVNAADALL
ANGAKDVYAYITHGVLSGGACARINSSKLKELVITDSIQPTNAVCNSANIRTLSIASLIAEAIGRTASEESVSSLFD
>Mature_316_residues
SWKNGSVKLVAGNSNPELARGIADWLKMPLTKASVRRFADNEVFVEIQENVRGSDVYIIQSTSFPTNDHLMELLIITDAL
RRASARRITAVIPYFGYARQDRKAGGRTPISAKLVANLITHAGTDRVMTLDLHAGQIQGFFDIPTDNLFASPVMVRDIKE
RFDLSKVAVVSPDVGGVVRARGLAKRINAPLAIIDKRRERAGESEVMNVIGEVEGYTCILIDDIVDSGGTLVNAADALLA
NGAKDVYAYITHGVLSGGACARINSSKLKELVITDSIQPTNAVCNSANIRTLSIASLIAEAIGRTASEESVSSLFD

Specific function: Utilized by both the de novo and the salvage pathways by which endogenously formed or exogenously added pyrimidine, purine, or pyridine bases are converted to the corresponding ribonucleoside monophosphates. [C]

COG id: COG0462

COG function: function code FE; Phosphoribosylpyrophosphate synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribose-phosphate pyrophosphokinase family [H]

Homologues:

Organism=Homo sapiens, GI4506127, Length=311, Percent_Identity=46.9453376205788, Blast_Score=291, Evalue=4e-79,
Organism=Homo sapiens, GI4506129, Length=311, Percent_Identity=46.6237942122186, Blast_Score=288, Evalue=5e-78,
Organism=Homo sapiens, GI28557709, Length=311, Percent_Identity=46.3022508038585, Blast_Score=286, Evalue=2e-77,
Organism=Homo sapiens, GI84875539, Length=314, Percent_Identity=46.1783439490446, Blast_Score=283, Evalue=2e-76,
Organism=Homo sapiens, GI4506133, Length=351, Percent_Identity=33.9031339031339, Blast_Score=173, Evalue=2e-43,
Organism=Homo sapiens, GI194018537, Length=332, Percent_Identity=35.8433734939759, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI310128524, Length=144, Percent_Identity=32.6388888888889, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI310115209, Length=144, Percent_Identity=32.6388888888889, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI310118259, Length=144, Percent_Identity=32.6388888888889, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI310119946, Length=144, Percent_Identity=32.6388888888889, Blast_Score=79, Evalue=4e-15,
Organism=Escherichia coli, GI1787458, Length=312, Percent_Identity=54.4871794871795, Blast_Score=345, Evalue=3e-96,
Organism=Caenorhabditis elegans, GI25149168, Length=311, Percent_Identity=47.588424437299, Blast_Score=298, Evalue=3e-81,
Organism=Caenorhabditis elegans, GI17554702, Length=311, Percent_Identity=47.588424437299, Blast_Score=298, Evalue=3e-81,
Organism=Caenorhabditis elegans, GI71989924, Length=311, Percent_Identity=47.588424437299, Blast_Score=296, Evalue=7e-81,
Organism=Caenorhabditis elegans, GI17554704, Length=311, Percent_Identity=47.588424437299, Blast_Score=295, Evalue=2e-80,
Organism=Caenorhabditis elegans, GI17570245, Length=335, Percent_Identity=34.0298507462687, Blast_Score=188, Evalue=3e-48,
Organism=Saccharomyces cerevisiae, GI6320946, Length=312, Percent_Identity=46.7948717948718, Blast_Score=276, Evalue=4e-75,
Organism=Saccharomyces cerevisiae, GI6319403, Length=314, Percent_Identity=46.4968152866242, Blast_Score=271, Evalue=8e-74,
Organism=Saccharomyces cerevisiae, GI6321776, Length=312, Percent_Identity=46.474358974359, Blast_Score=267, Evalue=2e-72,
Organism=Saccharomyces cerevisiae, GI6322667, Length=196, Percent_Identity=41.8367346938776, Blast_Score=155, Evalue=6e-39,
Organism=Saccharomyces cerevisiae, GI6324511, Length=101, Percent_Identity=41.5841584158416, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21355239, Length=311, Percent_Identity=47.2668810289389, Blast_Score=288, Evalue=4e-78,
Organism=Drosophila melanogaster, GI45551540, Length=334, Percent_Identity=44.6107784431138, Blast_Score=276, Evalue=2e-74,
Organism=Drosophila melanogaster, GI24651458, Length=351, Percent_Identity=33.3333333333333, Blast_Score=191, Evalue=8e-49,
Organism=Drosophila melanogaster, GI24651456, Length=351, Percent_Identity=33.3333333333333, Blast_Score=191, Evalue=8e-49,
Organism=Drosophila melanogaster, GI281362873, Length=351, Percent_Identity=33.3333333333333, Blast_Score=190, Evalue=1e-48,
Organism=Drosophila melanogaster, GI24651454, Length=351, Percent_Identity=33.3333333333333, Blast_Score=190, Evalue=1e-48,
Organism=Drosophila melanogaster, GI24651462, Length=199, Percent_Identity=38.1909547738693, Blast_Score=138, Evalue=6e-33,
Organism=Drosophila melanogaster, GI24651464, Length=199, Percent_Identity=38.1909547738693, Blast_Score=138, Evalue=6e-33,
Organism=Drosophila melanogaster, GI45552010, Length=199, Percent_Identity=38.1909547738693, Blast_Score=137, Evalue=8e-33,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000842
- InterPro:   IPR005946
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.7.6.1 [H]

Molecular weight: Translated: 34170; Mature: 34039

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSWKNGSVKLVAGNSNPELARGIADWLKMPLTKASVRRFADNEVFVEIQENVRGSDVYII
CCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEEEECCCCCCEEEEE
QSTSFPTNDHLMELLIITDALRRASARRITAVIPYFGYARQDRKAGGRTPISAKLVANLI
ECCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCHHHCCCCCCCHHHHHHHHHH
THAGTDRVMTLDLHAGQIQGFFDIPTDNLFASPVMVRDIKERFDLSKVAVVSPDVGGVVR
HHCCCCEEEEEEECCCCCCEEEECCCCCHHCCHHHHHHHHHHCCCCEEEEECCCCCCHHH
ARGLAKRINAPLAIIDKRRERAGESEVMNVIGEVEGYTCILIDDIVDSGGTLVNAADALL
HHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHCCCCEEEEEEEHHHCCCCCEEHHHHHHH
ANGAKDVYAYITHGVLSGGACARINSSKLKELVITDSIQPTNAVCNSANIRTLSIASLIA
HCCHHHHHHHHHHHHHCCCCEEECCHHHHHHHHEECCCCCHHHHHCCCCCEEHHHHHHHH
EAIGRTASEESVSSLFD
HHHCCCCCHHHHHHHCC
>Mature Secondary Structure 
SWKNGSVKLVAGNSNPELARGIADWLKMPLTKASVRRFADNEVFVEIQENVRGSDVYII
CCCCCCEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEEEECCCCCCEEEEE
QSTSFPTNDHLMELLIITDALRRASARRITAVIPYFGYARQDRKAGGRTPISAKLVANLI
ECCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCHHHCCCCCCCHHHHHHHHHH
THAGTDRVMTLDLHAGQIQGFFDIPTDNLFASPVMVRDIKERFDLSKVAVVSPDVGGVVR
HHCCCCEEEEEEECCCCCCEEEECCCCCHHCCHHHHHHHHHHCCCCEEEEECCCCCCHHH
ARGLAKRINAPLAIIDKRRERAGESEVMNVIGEVEGYTCILIDDIVDSGGTLVNAADALL
HHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHCCCCEEEEEEEHHHCCCCCEEHHHHHHH
ANGAKDVYAYITHGVLSGGACARINSSKLKELVITDSIQPTNAVCNSANIRTLSIASLIA
HCCHHHHHHHHHHHHHCCCCEEECCHHHHHHHHEECCCCCHHHHHCCCCCEEHHHHHHHH
EAIGRTASEESVSSLFD
HHHCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]