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The map label for this gene is rhtB [H]

Identifier: 86751244

GI number: 86751244

Start: 4711476

End: 4712093

Strand: Reverse

Name: rhtB [H]

Synonym: RPB_4137

Alternate gene names: 86751244

Gene position: 4712093-4711476 (Counterclockwise)

Preceding gene: 86751245

Following gene: 86751242

Centisome position: 88.38

GC content: 70.55

Gene sequence:

>618_bases
ATGTCGCTGCAGCTGTATCTCACCTATGTCTCCGCCTGCATCGTGCTGGCGCTGCTGCCGGGGCCGGTGGTGACGCTGGT
GATCGCCAACGGCCTGCGCCACGGCACCCGCGCGGCGCTCGCCAACATCGTCGGCGCGCAGCTCGGGCTGGGCCTCGTCA
TCGCGCTGGTCGCGGCGGGGCTGACCACGCTGATGGCGACGATGGGCTATTGGTTCGACTGGCTGCGCTTCCTCGGCGCC
GCCTATCTGGTGTGGCTCGGCATCGGCCTGATCCGCCATCCGGTCCAGGGCGTGGCGACCGACGCGCCGCCACCGCCGCC
GCGCGGCGGCTTCTTCCTGCAGGGCGTGGTGGTGCTGCTCGCCAATCCCAAGGTGCTGGTGTTCTTCGGCGCGTTCATCC
CGCAATTCGTCGACATGCAGCGCGATCACGTCCCGCAGGTGGCGCTGCTGGGGGCGACCTTCATGGCGATCGTCGCCGTC
ACCGACGCGGCCTACGCGTTGCTGGCGGGCCGGGCGCGCACGATGTTCTCGGCGCGCCGCACCCGGCTGCTGTCGCGGAT
TTCCGGTGGCTTCATGATCGGCGGCGGCGTTTGGCTCGCGCTCAGCCGGGCGAAGTGA

Upstream 100 bases:

>100_bases
CTCGGCGTGTCGCGCGCCGGCGGGATGATGCCCGACCGGCTCGCGCCGCCGCCGCGCGGCTGCTATCATTCGCGACCTTT
GATCGCGAATGGGAAGGCCG

Downstream 100 bases:

>100_bases
GGCGCCTTGTTCGGCAACACGCGCGGACGTCATCCTGAGGAGCGAGCGCAGCGAGCCTCGAAGGATGTCGGAGACGGGCA
CCGTGTCTCATCCTTCGAGG

Product: lysine exporter protein LysE/YggA

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 205; Mature: 204

Protein sequence:

>205_residues
MSLQLYLTYVSACIVLALLPGPVVTLVIANGLRHGTRAALANIVGAQLGLGLVIALVAAGLTTLMATMGYWFDWLRFLGA
AYLVWLGIGLIRHPVQGVATDAPPPPPRGGFFLQGVVVLLANPKVLVFFGAFIPQFVDMQRDHVPQVALLGATFMAIVAV
TDAAYALLAGRARTMFSARRTRLLSRISGGFMIGGGVWLALSRAK

Sequences:

>Translated_205_residues
MSLQLYLTYVSACIVLALLPGPVVTLVIANGLRHGTRAALANIVGAQLGLGLVIALVAAGLTTLMATMGYWFDWLRFLGA
AYLVWLGIGLIRHPVQGVATDAPPPPPRGGFFLQGVVVLLANPKVLVFFGAFIPQFVDMQRDHVPQVALLGATFMAIVAV
TDAAYALLAGRARTMFSARRTRLLSRISGGFMIGGGVWLALSRAK
>Mature_204_residues
SLQLYLTYVSACIVLALLPGPVVTLVIANGLRHGTRAALANIVGAQLGLGLVIALVAAGLTTLMATMGYWFDWLRFLGAA
YLVWLGIGLIRHPVQGVATDAPPPPPRGGFFLQGVVVLLANPKVLVFFGAFIPQFVDMQRDHVPQVALLGATFMAIVAVT
DAAYALLAGRARTMFSARRTRLLSRISGGFMIGGGVWLALSRAK

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=204, Percent_Identity=34.3137254901961, Blast_Score=98, Evalue=4e-22,
Organism=Escherichia coli, GI1788099, Length=179, Percent_Identity=26.8156424581006, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI48994978, Length=210, Percent_Identity=26.6666666666667, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 21742; Mature: 21611

Theoretical pI: Translated: 11.59; Mature: 11.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLQLYLTYVSACIVLALLPGPVVTLVIANGLRHGTRAALANIVGAQLGLGLVIALVAAG
CCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTTLMATMGYWFDWLRFLGAAYLVWLGIGLIRHPVQGVATDAPPPPPRGGFFLQGVVVLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCCCCCCCCHHHHHHHHHH
ANPKVLVFFGAFIPQFVDMQRDHVPQVALLGATFMAIVAVTDAAYALLAGRARTMFSARR
CCCCCEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TRLLSRISGGFMIGGGVWLALSRAK
HHHHHHHCCCEEECCCEEEEEECCC
>Mature Secondary Structure 
SLQLYLTYVSACIVLALLPGPVVTLVIANGLRHGTRAALANIVGAQLGLGLVIALVAAG
CHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTTLMATMGYWFDWLRFLGAAYLVWLGIGLIRHPVQGVATDAPPPPPRGGFFLQGVVVLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCCCCCCCCHHHHHHHHHH
ANPKVLVFFGAFIPQFVDMQRDHVPQVALLGATFMAIVAVTDAAYALLAGRARTMFSARR
CCCCCEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TRLLSRISGGFMIGGGVWLALSRAK
HHHHHHHCCCEEECCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]