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The map label for this gene is dnaG [H]

Identifier: 86751239

GI number: 86751239

Start: 4705991

End: 4708042

Strand: Reverse

Name: dnaG [H]

Synonym: RPB_4132

Alternate gene names: 86751239

Gene position: 4708042-4705991 (Counterclockwise)

Preceding gene: 86751241

Following gene: 86751238

Centisome position: 88.3

GC content: 68.62

Gene sequence:

>2052_bases
ATGCGCTTCACGCCGCAATTTCTCGACGAGTTGAAAGCCCGGCTGCTGGTGTCGGAGGTGGTCGGCCGCCGGGTCAAGCT
GAAGAAGGCCGGGCGGGAGTGGAAGGGGCTTTCGCCCTTTCAACAAGAGAAGACGCCGTCGTTCTTCGTCAATGACGAGA
AGCAGGCCTGGTTCGACTTCTCCTCCGGCAAGAACGGCTCGATCTTCGACTTCATCATGCAGACCGACGGGGTGGATTTC
CCCGAAGCGGTCGAGCGGCTCGCCGCGATGGCGGGCCTCGCCTTGCCGGCCGCGACGCCCGACGCCGCGCGCCAGGAGCA
GCGCCGCCGCTCGCTGTACGACGTCATGGAAATGGCGGCGGTGTATTTCTCCGAGAATCTCGCCTCGCGCGCCGGCGCCC
GGGCCCGCGGCTATCTCGCCGATCGCGGCATCCTGCCGTCGACCCAGGTCAAATTCCGCATCGGCTATGCCTCGCCGGAT
CGCTTCGCGCTCAAGGAGCATCTCGGCAAGCACGGCGTGTCGGTCGACGACATGGTCGAGGCCGGGCTGTTGTCGGCCGG
CGACGACATCCCGGTGCCGTTCGACAAGTTTCGCGACCGGGTGATGTTTCCGATCACCGACATCCGCGGCCGGGTGATCG
CCTTCGGCGGTCGCGCGCTGGAGAAGGACGTCGCGGCGAAGTATCTGAACTCGCCGGAAACCCCGCTGTTTCACAAGGGC
GACAACCTCTACAACCTCGCCACCGCGCGCAAGGCGGCGCATGACGCGGCCGGCAAGTCGACGCCGACCGGCGCCGCGTC
GGTGATCGTGGTCGAGGGCTATATCGACGTCATCGCCATGGTCACGGCGGGCTTCGCAGCCACCGTCGCGCCGCTCGGCA
CCGCGCTGACCGAGAACCAGCTTCAGCTGCTGTGGAAGATGGCGGACGAGCCGATCCTGTGTTTCGACGGCGACCGCGCC
GGCCAGAAGGCGGCATATCGCGCCGCCGATCTGGCGCTGCCGCATCTCAAGCCCGGCAAGAGCCTGCGCTTCGCCCTCTT
GCCCGAGGGCCAGGATCCCGACGATCTCGCCCGCGCCGGCGGGCGGCTAGCGATCGAGGAGGTGATCGGCGCGGCGCGGC
CGCTGGCCGACCTGCTGTGGTCGCGCGAGGCCGAAGGCGGCAGTTTCGCCACGCCGGAGCGGCGCGCGGCGCTGGAGGCG
CGGATCAACGAGCTCGCCAACGGCATCCGCGACGAGGTGGTGCGGCGCTACTATCGGAAGGACTGGGCCGAGCGGCTGCG
CGCGGCCTTCGCGCCGCAGGGTGGCTTCGGCGGAGGGTTCGGCGGAGGCTTCCGGGCCGGCGGCGGCGCGCGCGGTGGTG
CTGATTCGGGGCGCCGGTTTCAGCCGCGCGGGAGTTTTCCACAGGGCGGCGGCCGTTATGCACCGTTCGGCCGGCAGACC
GGCCTCAGTTCGCCGCCGCAGGGGCGCGGGCCGTATCAGGTCGCAAGCCCCGATCTGGCGTCGAGCCCGATCATGCGCGG
GCAGCGCAGCGCGCTGTCGCGCCGCGAGGCGCTGATCCTGCAATCGCTGCTCAACCACCCCTGGCTGCTGCACGATCATC
TCGAAGAAGTCGCGGCGCTGGAATTCGCCCATCCGGAGGCGCACCGGCTGCGCGCGGCGATCATCGCCGCCTTCGCGGCC
GACCATCATCACGGCGACGATCCGGCCGGACAGTCCGAGAAACTTCGCGCCGATCTCGCGAAGGCCGGTTTATCCGAGCA
ATTGCAACGGCTTGAACGCGCGATCACGACCCACGCGGTGTGGGGCGCGGGGCCGGACGCGGCGCCCGAGGACGTTCTTT
CGACGTGGCAGCAACTCGTTGCCTTGCATCGACAATCTCACTCCTTACTTAGGGAGCTGAAGGATGCCGAGCTGGCTTTG
GGTGAAGACGGCAGCGAGGCCAACGTCGCCTGGTTGAGGGACGTCAAGGCGCGGCTCTCGGAAGTGGAAGGGACTGAGGC
GCTGATCGAGGGGTTCGGCCAGTCATCGGGCCGGTTCCATCGGAGCGTGTGA

Upstream 100 bases:

>100_bases
AAAGCCGGTATAAGCCAATCGAACTTCGGCATTGCCGGGGCGAGACCTATATCCCTAATCGGTTGGCGCCATCGCGCCCG
ACCCAGAGTCTCCGCACCCC

Downstream 100 bases:

>100_bases
CCGGGTTCGAGGCGGTCGGGTGACGGCGGGTGCCGTGAAAAGACTCGCCAAACCCGCACATTGGCTGCAAAAACAGGGTT
AGTAAGGGCTTAACGGCATT

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 683; Mature: 683

Protein sequence:

>683_residues
MRFTPQFLDELKARLLVSEVVGRRVKLKKAGREWKGLSPFQQEKTPSFFVNDEKQAWFDFSSGKNGSIFDFIMQTDGVDF
PEAVERLAAMAGLALPAATPDAARQEQRRRSLYDVMEMAAVYFSENLASRAGARARGYLADRGILPSTQVKFRIGYASPD
RFALKEHLGKHGVSVDDMVEAGLLSAGDDIPVPFDKFRDRVMFPITDIRGRVIAFGGRALEKDVAAKYLNSPETPLFHKG
DNLYNLATARKAAHDAAGKSTPTGAASVIVVEGYIDVIAMVTAGFAATVAPLGTALTENQLQLLWKMADEPILCFDGDRA
GQKAAYRAADLALPHLKPGKSLRFALLPEGQDPDDLARAGGRLAIEEVIGAARPLADLLWSREAEGGSFATPERRAALEA
RINELANGIRDEVVRRYYRKDWAERLRAAFAPQGGFGGGFGGGFRAGGGARGGADSGRRFQPRGSFPQGGGRYAPFGRQT
GLSSPPQGRGPYQVASPDLASSPIMRGQRSALSRREALILQSLLNHPWLLHDHLEEVAALEFAHPEAHRLRAAIIAAFAA
DHHHGDDPAGQSEKLRADLAKAGLSEQLQRLERAITTHAVWGAGPDAAPEDVLSTWQQLVALHRQSHSLLRELKDAELAL
GEDGSEANVAWLRDVKARLSEVEGTEALIEGFGQSSGRFHRSV

Sequences:

>Translated_683_residues
MRFTPQFLDELKARLLVSEVVGRRVKLKKAGREWKGLSPFQQEKTPSFFVNDEKQAWFDFSSGKNGSIFDFIMQTDGVDF
PEAVERLAAMAGLALPAATPDAARQEQRRRSLYDVMEMAAVYFSENLASRAGARARGYLADRGILPSTQVKFRIGYASPD
RFALKEHLGKHGVSVDDMVEAGLLSAGDDIPVPFDKFRDRVMFPITDIRGRVIAFGGRALEKDVAAKYLNSPETPLFHKG
DNLYNLATARKAAHDAAGKSTPTGAASVIVVEGYIDVIAMVTAGFAATVAPLGTALTENQLQLLWKMADEPILCFDGDRA
GQKAAYRAADLALPHLKPGKSLRFALLPEGQDPDDLARAGGRLAIEEVIGAARPLADLLWSREAEGGSFATPERRAALEA
RINELANGIRDEVVRRYYRKDWAERLRAAFAPQGGFGGGFGGGFRAGGGARGGADSGRRFQPRGSFPQGGGRYAPFGRQT
GLSSPPQGRGPYQVASPDLASSPIMRGQRSALSRREALILQSLLNHPWLLHDHLEEVAALEFAHPEAHRLRAAIIAAFAA
DHHHGDDPAGQSEKLRADLAKAGLSEQLQRLERAITTHAVWGAGPDAAPEDVLSTWQQLVALHRQSHSLLRELKDAELAL
GEDGSEANVAWLRDVKARLSEVEGTEALIEGFGQSSGRFHRSV
>Mature_683_residues
MRFTPQFLDELKARLLVSEVVGRRVKLKKAGREWKGLSPFQQEKTPSFFVNDEKQAWFDFSSGKNGSIFDFIMQTDGVDF
PEAVERLAAMAGLALPAATPDAARQEQRRRSLYDVMEMAAVYFSENLASRAGARARGYLADRGILPSTQVKFRIGYASPD
RFALKEHLGKHGVSVDDMVEAGLLSAGDDIPVPFDKFRDRVMFPITDIRGRVIAFGGRALEKDVAAKYLNSPETPLFHKG
DNLYNLATARKAAHDAAGKSTPTGAASVIVVEGYIDVIAMVTAGFAATVAPLGTALTENQLQLLWKMADEPILCFDGDRA
GQKAAYRAADLALPHLKPGKSLRFALLPEGQDPDDLARAGGRLAIEEVIGAARPLADLLWSREAEGGSFATPERRAALEA
RINELANGIRDEVVRRYYRKDWAERLRAAFAPQGGFGGGFGGGFRAGGGARGGADSGRRFQPRGSFPQGGGRYAPFGRQT
GLSSPPQGRGPYQVASPDLASSPIMRGQRSALSRREALILQSLLNHPWLLHDHLEEVAALEFAHPEAHRLRAAIIAAFAA
DHHHGDDPAGQSEKLRADLAKAGLSEQLQRLERAITTHAVWGAGPDAAPEDVLSTWQQLVALHRQSHSLLRELKDAELAL
GEDGSEANVAWLRDVKARLSEVEGTEALIEGFGQSSGRFHRSV

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=422, Percent_Identity=39.3364928909953, Blast_Score=296, Evalue=3e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016136
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR013173
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF08278 DnaG_DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 74172; Mature: 74172

Theoretical pI: Translated: 7.98; Mature: 7.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFTPQFLDELKARLLVSEVVGRRVKLKKAGREWKGLSPFQQEKTPSFFVNDEKQAWFDF
CCCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCHHHHCCCCEEECCCCHHEEEC
SSGKNGSIFDFIMQTDGVDFPEAVERLAAMAGLALPAATPDAARQEQRRRSLYDVMEMAA
CCCCCCCCEEEHHHCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
VYFSENLASRAGARARGYLADRGILPSTQVKFRIGYASPDRFALKEHLGKHGVSVDDMVE
HHHHHHHHHHCCCCCCCCHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCHHHHHH
AGLLSAGDDIPVPFDKFRDRVMFPITDIRGRVIAFGGRALEKDVAAKYLNSPETPLFHKG
HHHHCCCCCCCCCHHHHCCCEECCCCCCCCCEEEECCHHHHHHHHHHHHCCCCCCCEECC
DNLYNLATARKAAHDAAGKSTPTGAASVIVVEGYIDVIAMVTAGFAATVAPLGTALTENQ
CCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQLLWKMADEPILCFDGDRAGQKAAYRAADLALPHLKPGKSLRFALLPEGQDPDDLARAG
HHHHHHHCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHHHCC
GRLAIEEVIGAARPLADLLWSREAEGGSFATPERRAALEARINELANGIRDEVVRRYYRK
CCEEHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DWAERLRAAFAPQGGFGGGFGGGFRAGGGARGGADSGRRFQPRGSFPQGGGRYAPFGRQT
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GLSSPPQGRGPYQVASPDLASSPIMRGQRSALSRREALILQSLLNHPWLLHDHLEEVAAL
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
EFAHPEAHRLRAAIIAAFAADHHHGDDPAGQSEKLRADLAKAGLSEQLQRLERAITTHAV
HHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WGAGPDAAPEDVLSTWQQLVALHRQSHSLLRELKDAELALGEDGSEANVAWLRDVKARLS
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
EVEGTEALIEGFGQSSGRFHRSV
HCCCHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MRFTPQFLDELKARLLVSEVVGRRVKLKKAGREWKGLSPFQQEKTPSFFVNDEKQAWFDF
CCCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCHHHHCCCCEEECCCCHHEEEC
SSGKNGSIFDFIMQTDGVDFPEAVERLAAMAGLALPAATPDAARQEQRRRSLYDVMEMAA
CCCCCCCCEEEHHHCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
VYFSENLASRAGARARGYLADRGILPSTQVKFRIGYASPDRFALKEHLGKHGVSVDDMVE
HHHHHHHHHHCCCCCCCCHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCHHHHHH
AGLLSAGDDIPVPFDKFRDRVMFPITDIRGRVIAFGGRALEKDVAAKYLNSPETPLFHKG
HHHHCCCCCCCCCHHHHCCCEECCCCCCCCCEEEECCHHHHHHHHHHHHCCCCCCCEECC
DNLYNLATARKAAHDAAGKSTPTGAASVIVVEGYIDVIAMVTAGFAATVAPLGTALTENQ
CCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQLLWKMADEPILCFDGDRAGQKAAYRAADLALPHLKPGKSLRFALLPEGQDPDDLARAG
HHHHHHHCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHHHCC
GRLAIEEVIGAARPLADLLWSREAEGGSFATPERRAALEARINELANGIRDEVVRRYYRK
CCEEHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DWAERLRAAFAPQGGFGGGFGGGFRAGGGARGGADSGRRFQPRGSFPQGGGRYAPFGRQT
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GLSSPPQGRGPYQVASPDLASSPIMRGQRSALSRREALILQSLLNHPWLLHDHLEEVAAL
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
EFAHPEAHRLRAAIIAAFAADHHHGDDPAGQSEKLRADLAKAGLSEQLQRLERAITTHAV
HHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WGAGPDAAPEDVLSTWQQLVALHRQSHSLLRELKDAELALGEDGSEANVAWLRDVKARLS
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
EVEGTEALIEGFGQSSGRFHRSV
HCCCHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]