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The map label for this gene is mdlC [H]

Identifier: 86751046

GI number: 86751046

Start: 4493600

End: 4495222

Strand: Direct

Name: mdlC [H]

Synonym: RPB_3938

Alternate gene names: 86751046

Gene position: 4493600-4495222 (Clockwise)

Preceding gene: 86751045

Following gene: 86751049

Centisome position: 84.28

GC content: 69.32

Gene sequence:

>1623_bases
TTGCCGAAGAAGCCGAAGCCGAGCGCTGCCGTCAGCACCGTCAAATCCGCCACGCTCGATCTGCTCCGCGCGTTCAAGAT
CGACAAGGTGTTCGGCAATCCCGGCTCCACCGAGCTGCCGTTCCTCAGCGACTGGCCGGACGACATCGACTACGTACTGG
CGCTGCAGGAGGCGAGCGCGATGGCGATGGCCGACGGCTACGCGCAGGCGACGCGCAACGCCGGCTTCGTCAATCTGCAT
TCAGCCGCCGGCGTCGGCAACGCGCTCGGCAACATCTACACCGCGTTCAAGAACCAGACGCCGCTGCTGATCACCGCCGG
CCAGCAGGCGCGCAGCCTGCTGCCGTTACAGGCGTTTCTCGGCGCCGAACGCGCATCCGAATTTCCGCGGCCTTACGTGA
AATACAGCATCGAGCCGGCGCGCGCCGAGGACGTGCCGGCGGCGATCGCCCGCGCCTATTATGTGGCGATGCAGCCGCCG
TGCGGGCCGACCTTCGTGTCGGTGCCGATCGACGACTGGGCGCGGCCCGCGCAGCCGGTTCCGCTGCGCAACGTGACGCG
CGAACTCGGCCCGGAGCGCGCGGCGATGCAGGCGCTGGCGGAGGCGCTGGCGGAGGCGAAGAAGCCCGCCCTGGTGGTCG
GCCCCGCGATCGATCGCGCCGCCGCGGTCGATTTGATGGTGCGCCTCGCCGAGCGCGCCAACGCGCCGGTGTTCGTCAGT
CCGTTCTCGGCGCGCTGCAGTTTCCCGGAGCGGCATCCGCTGTTCGCCGGCTTCCTGCCCGCCTCGCCGGGGCAACTCTC
CGAAGCCATCGGCGCCTACGACGTCGTGGTGGTGATCGGCGCACCGGTGTTCACCTTCCATGTCGAAGGCCGCGCGTCGA
TCTTCGACGGCGCAACGTCGCTGTTCCAGATCACCGACGACGCCGAGGCCGCGTCGGTGACGCCGCTCGGCACCAGCATC
ATCGCCACCATGAAGCCGGCATTATCGCTGCTGCTGGAGTTGTTACCAGAGACCCAATGCGCGGCGCCGCCGGCACGGGC
GCTGCCGCCAGCGCCTGCCGCGGCCGATCCGATGCCGGCCGAATTTCTGCTCGATGCGTTGAGCAAGGCGATGCCGGCCG
GCACGATGCTGGTCGAGGAAGCGCCGTCGCATCGGCTGGCGATGCAGAAATTCATGCCGATGCGCGGCCAGGACAGTTTC
GCCACGATGGCGAGCGGCGGCCTCGGCTGGTCGCTGCCGGCCGCGGTCGGCTTCGCGCTGGCGCATCCGGAGCGCCGCAC
CGTGTGCCTGATCGGCGACGGCTCGGCGATGTATTCGATCCAGGCGCTGTGGACTGCGGCAGAGCGCAAGCTGCCGCTGA
CCGTGGTGGTGCTGAACAATGGCGGCTACGGCGCGATGCGCTCGTTCAGCCAGGTGATGCAGGTCCGCGACGTGCCCGGG
CTGGAGCTGCCCGGGATCGACTACGTCCAGCTCGCGCAGTCGATGGGCTGTGTCGCCGAACGCGTGTCACGCTGTGAGGA
CCTCGCGCCGGTGCTCGCCCGCGCGCTGGCGCATGACGGCGTGTTCGTGGTCGAGGCGACGCTGGATAGCGCGGTGCCGC
TGCTGTACGCGAAGAACGGGTAG

Upstream 100 bases:

>100_bases
AGGCCCGTACTGCCTGCACAGGCTGTTGACTCCCCTCGCCTCCTGCTGATTGATCGGCGCAAGCAACAATCACGGCGCGC
CGCCGCCGGAGGAAACCGCC

Downstream 100 bases:

>100_bases
GTCGTCGCAACATGATCCGCAGCGCCCGCAACGAGCACGGCGAGCTCCCTCGCCCCGCTCTTGCGGGGAGAGGGTTGGGG
TGAGGGGCGAGACCGGTCGC

Product: benzoylformate decarboxylase

Products: NA

Alternate protein names: BFD; BFDC [H]

Number of amino acids: Translated: 540; Mature: 539

Protein sequence:

>540_residues
MPKKPKPSAAVSTVKSATLDLLRAFKIDKVFGNPGSTELPFLSDWPDDIDYVLALQEASAMAMADGYAQATRNAGFVNLH
SAAGVGNALGNIYTAFKNQTPLLITAGQQARSLLPLQAFLGAERASEFPRPYVKYSIEPARAEDVPAAIARAYYVAMQPP
CGPTFVSVPIDDWARPAQPVPLRNVTRELGPERAAMQALAEALAEAKKPALVVGPAIDRAAAVDLMVRLAERANAPVFVS
PFSARCSFPERHPLFAGFLPASPGQLSEAIGAYDVVVVIGAPVFTFHVEGRASIFDGATSLFQITDDAEAASVTPLGTSI
IATMKPALSLLLELLPETQCAAPPARALPPAPAAADPMPAEFLLDALSKAMPAGTMLVEEAPSHRLAMQKFMPMRGQDSF
ATMASGGLGWSLPAAVGFALAHPERRTVCLIGDGSAMYSIQALWTAAERKLPLTVVVLNNGGYGAMRSFSQVMQVRDVPG
LELPGIDYVQLAQSMGCVAERVSRCEDLAPVLARALAHDGVFVVEATLDSAVPLLYAKNG

Sequences:

>Translated_540_residues
MPKKPKPSAAVSTVKSATLDLLRAFKIDKVFGNPGSTELPFLSDWPDDIDYVLALQEASAMAMADGYAQATRNAGFVNLH
SAAGVGNALGNIYTAFKNQTPLLITAGQQARSLLPLQAFLGAERASEFPRPYVKYSIEPARAEDVPAAIARAYYVAMQPP
CGPTFVSVPIDDWARPAQPVPLRNVTRELGPERAAMQALAEALAEAKKPALVVGPAIDRAAAVDLMVRLAERANAPVFVS
PFSARCSFPERHPLFAGFLPASPGQLSEAIGAYDVVVVIGAPVFTFHVEGRASIFDGATSLFQITDDAEAASVTPLGTSI
IATMKPALSLLLELLPETQCAAPPARALPPAPAAADPMPAEFLLDALSKAMPAGTMLVEEAPSHRLAMQKFMPMRGQDSF
ATMASGGLGWSLPAAVGFALAHPERRTVCLIGDGSAMYSIQALWTAAERKLPLTVVVLNNGGYGAMRSFSQVMQVRDVPG
LELPGIDYVQLAQSMGCVAERVSRCEDLAPVLARALAHDGVFVVEATLDSAVPLLYAKNG
>Mature_539_residues
PKKPKPSAAVSTVKSATLDLLRAFKIDKVFGNPGSTELPFLSDWPDDIDYVLALQEASAMAMADGYAQATRNAGFVNLHS
AAGVGNALGNIYTAFKNQTPLLITAGQQARSLLPLQAFLGAERASEFPRPYVKYSIEPARAEDVPAAIARAYYVAMQPPC
GPTFVSVPIDDWARPAQPVPLRNVTRELGPERAAMQALAEALAEAKKPALVVGPAIDRAAAVDLMVRLAERANAPVFVSP
FSARCSFPERHPLFAGFLPASPGQLSEAIGAYDVVVVIGAPVFTFHVEGRASIFDGATSLFQITDDAEAASVTPLGTSII
ATMKPALSLLLELLPETQCAAPPARALPPAPAAADPMPAEFLLDALSKAMPAGTMLVEEAPSHRLAMQKFMPMRGQDSFA
TMASGGLGWSLPAAVGFALAHPERRTVCLIGDGSAMYSIQALWTAAERKLPLTVVVLNNGGYGAMRSFSQVMQVRDVPGL
ELPGIDYVQLAQSMGCVAERVSRCEDLAPVLARALAHDGVFVVEATLDSAVPLLYAKNG

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=511, Percent_Identity=24.0704500978474, Blast_Score=101, Evalue=2e-21,
Organism=Escherichia coli, GI87081685, Length=537, Percent_Identity=26.0707635009311, Blast_Score=152, Evalue=4e-38,
Organism=Escherichia coli, GI1790104, Length=511, Percent_Identity=25.6360078277886, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1786717, Length=539, Percent_Identity=23.747680890538, Blast_Score=123, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6323755, Length=560, Percent_Identity=24.8214285714286, Blast_Score=120, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6321524, Length=487, Percent_Identity=22.5872689938398, Blast_Score=95, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6323073, Length=527, Percent_Identity=22.5806451612903, Blast_Score=88, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6323163, Length=522, Percent_Identity=22.6053639846743, Blast_Score=88, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6320123, Length=521, Percent_Identity=21.8809980806142, Blast_Score=86, Evalue=2e-17,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =4.1.1.7 [H]

Molecular weight: Translated: 57045; Mature: 56914

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKKPKPSAAVSTVKSATLDLLRAFKIDKVFGNPGSTELPFLSDWPDDIDYVLALQEASA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHEEHHHHHH
MAMADGYAQATRNAGFVNLHSAAGVGNALGNIYTAFKNQTPLLITAGQQARSLLPLQAFL
HHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHH
GAERASEFPRPYVKYSIEPARAEDVPAAIARAYYVAMQPPCGPTFVSVPIDDWARPAQPV
CHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHEEEEECCCCCCCEEEECCHHCCCCCCCC
PLRNVTRELGPERAAMQALAEALAEAKKPALVVGPAIDRAAAVDLMVRLAERANAPVFVS
CHHHHHHHHCHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCEEEC
PFSARCSFPERHPLFAGFLPASPGQLSEAIGAYDVVVVIGAPVFTFHVEGRASIFDGATS
CCCCCCCCCCCCCCEEECCCCCCHHHHHHHCCEEEEEEECCCEEEEEECCCCHHHHCCCE
LFQITDDAEAASVTPLGTSIIATMKPALSLLLELLPETQCAAPPARALPPAPAAADPMPA
EEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCH
EFLLDALSKAMPAGTMLVEEAPSHRLAMQKFMPMRGQDSFATMASGGLGWSLPAAVGFAL
HHHHHHHHHHCCCCCEEECCCCCHHHHHHHHCCCCCCCCHHHHHCCCCCCCHHHHHHHHH
AHPERRTVCLIGDGSAMYSIQALWTAAERKLPLTVVVLNNGGYGAMRSFSQVMQVRDVPG
CCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCC
LELPGIDYVQLAQSMGCVAERVSRCEDLAPVLARALAHDGVFVVEATLDSAVPLLYAKNG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEECCC
>Mature Secondary Structure 
PKKPKPSAAVSTVKSATLDLLRAFKIDKVFGNPGSTELPFLSDWPDDIDYVLALQEASA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHEEHHHHHH
MAMADGYAQATRNAGFVNLHSAAGVGNALGNIYTAFKNQTPLLITAGQQARSLLPLQAFL
HHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHH
GAERASEFPRPYVKYSIEPARAEDVPAAIARAYYVAMQPPCGPTFVSVPIDDWARPAQPV
CHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHEEEEECCCCCCCEEEECCHHCCCCCCCC
PLRNVTRELGPERAAMQALAEALAEAKKPALVVGPAIDRAAAVDLMVRLAERANAPVFVS
CHHHHHHHHCHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCEEEC
PFSARCSFPERHPLFAGFLPASPGQLSEAIGAYDVVVVIGAPVFTFHVEGRASIFDGATS
CCCCCCCCCCCCCCEEECCCCCCHHHHHHHCCEEEEEEECCCEEEEEECCCCHHHHCCCE
LFQITDDAEAASVTPLGTSIIATMKPALSLLLELLPETQCAAPPARALPPAPAAADPMPA
EEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCH
EFLLDALSKAMPAGTMLVEEAPSHRLAMQKFMPMRGQDSFATMASGGLGWSLPAAVGFAL
HHHHHHHHHHCCCCCEEECCCCCHHHHHHHHCCCCCCCCHHHHHCCCCCCCHHHHHHHHH
AHPERRTVCLIGDGSAMYSIQALWTAAERKLPLTVVVLNNGGYGAMRSFSQVMQVRDVPG
CCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCC
LELPGIDYVQLAQSMGCVAERVSRCEDLAPVLARALAHDGVFVVEATLDSAVPLLYAKNG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2271624; 7663351; 9665697 [H]