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The map label for this gene is hpcB [H]

Identifier: 86750976

GI number: 86750976

Start: 4422829

End: 4423680

Strand: Direct

Name: hpcB [H]

Synonym: RPB_3868

Alternate gene names: 86750976

Gene position: 4422829-4423680 (Clockwise)

Preceding gene: 86750975

Following gene: 86750977

Centisome position: 82.95

GC content: 66.08

Gene sequence:

>852_bases
ATGGGCAAACTGGTGCTCGCCGCGAAGGTGACCCACGTTCCCTCGTTGATGTTGTCGGAGGCCGGCGACAGTCCACTGAA
GCAGGCGCGCGCCGGCGCGGTGGAGTCGCTGCGCGAACTCGGCCGACGCGCCAGGACCCGCGACGTCTCCACCTTCGTGG
TGTTCGACACCCATTGGCTGTCGAATTTCGGCTACCACGTCAACGCCAATGCCCGGCATCGCGGCTCCTTCACCAGCCAC
GAAGCGCCGCACATGATCCAGGATCTGCGCTACGATCTGCCGGGCGACACCGCGCTCGCCGAAGCGATCGCCAGCGAGGC
GGAGAGCGCCGGTCTCAAGGTGCTCGCGCATCAAGTTCCGACGCTGGGGCTGGAGTACGGCACCATCGTGCCGATGCACT
ATCTGAATCCCGACGGCTGGGCCAAGGTGGTGTCGATCGCCTCGCCGTTGTTCACCTCGATCGAGGAAAGCCGCGCGCTC
GGCGAGGCGACGCGGCGCGCGATCGATCAGTCCGGCGAGCGAGTGGCTCTTCTCGCCAGCGGATCGCTGTCGCATCGGCT
GTGGCCGAACAAAAACCTCGGCCCGGAGGCCTGGACCTCGATCGCCAGTGAGTTCAATCGCCAGGTCGACCTGCGCGTGC
TGCAACTCTGGCAGGAGCGGCGCTACCGCGAATTCACCGCGATGCTCCCGGACTATGCGGTGAAGTGCAACGGCGAGGGC
GGCATGGGCGACACCGTGATGCTGTTCGCCGCGCTCGGCTGGGACAAGTACGACGGCGAGGCGGAGCCGCTTTGCGACTA
CTTTCCGTCGAGCGGCAGCGGGCAGATCAACGTCGAATTCCATGTCGCGTGA

Upstream 100 bases:

>100_bases
TGGGCGGAGACCAAGAAACCGCCGCGCCACACCGCCTGGCTGCACCAGAAAGAATTCGCCGACGCCGAGTGATCTTGCCC
CCGCGACGATGGAGTGAAGC

Downstream 100 bases:

>100_bases
TCTGCGAATGCCTGCCGTTGTCGAGGTCACGGCCCTCCACGCTTGACAAACCGCCCCGCGGGACGGCGAAATCATCGCGA
AACTCGCACGATCCGCTGCC

Product: 3,4-dihydroxyphenylacetate 2,3-dioxygenase HpaD_Fe

Products: NA

Alternate protein names: Homoprotocatechuate 2,3-dioxygenase; HPC dioxygenase [H]

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MGKLVLAAKVTHVPSLMLSEAGDSPLKQARAGAVESLRELGRRARTRDVSTFVVFDTHWLSNFGYHVNANARHRGSFTSH
EAPHMIQDLRYDLPGDTALAEAIASEAESAGLKVLAHQVPTLGLEYGTIVPMHYLNPDGWAKVVSIASPLFTSIEESRAL
GEATRRAIDQSGERVALLASGSLSHRLWPNKNLGPEAWTSIASEFNRQVDLRVLQLWQERRYREFTAMLPDYAVKCNGEG
GMGDTVMLFAALGWDKYDGEAEPLCDYFPSSGSGQINVEFHVA

Sequences:

>Translated_283_residues
MGKLVLAAKVTHVPSLMLSEAGDSPLKQARAGAVESLRELGRRARTRDVSTFVVFDTHWLSNFGYHVNANARHRGSFTSH
EAPHMIQDLRYDLPGDTALAEAIASEAESAGLKVLAHQVPTLGLEYGTIVPMHYLNPDGWAKVVSIASPLFTSIEESRAL
GEATRRAIDQSGERVALLASGSLSHRLWPNKNLGPEAWTSIASEFNRQVDLRVLQLWQERRYREFTAMLPDYAVKCNGEG
GMGDTVMLFAALGWDKYDGEAEPLCDYFPSSGSGQINVEFHVA
>Mature_282_residues
GKLVLAAKVTHVPSLMLSEAGDSPLKQARAGAVESLRELGRRARTRDVSTFVVFDTHWLSNFGYHVNANARHRGSFTSHE
APHMIQDLRYDLPGDTALAEAIASEAESAGLKVLAHQVPTLGLEYGTIVPMHYLNPDGWAKVVSIASPLFTSIEESRALG
EATRRAIDQSGERVALLASGSLSHRLWPNKNLGPEAWTSIASEFNRQVDLRVLQLWQERRYREFTAMLPDYAVKCNGEGG
MGDTVMLFAALGWDKYDGEAEPLCDYFPSSGSGQINVEFHVA

Specific function: Transforms homoprotocatechuic acid (HPC) into 5- carboxymethyl-2-hydroxy-muconic semialdehyde (CHMS) [H]

COG id: COG3384

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011984
- InterPro:   IPR004183 [H]

Pfam domain/function: PF02900 LigB [H]

EC number: =1.13.11.15 [H]

Molecular weight: Translated: 31096; Mature: 30965

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKLVLAAKVTHVPSLMLSEAGDSPLKQARAGAVESLRELGRRARTRDVSTFVVFDTHWL
CCCEEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECHHH
SNFGYHVNANARHRGSFTSHEAPHMIQDLRYDLPGDTALAEAIASEAESAGLKVLAHQVP
HCCCEEEECCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHCC
TLGLEYGTIVPMHYLNPDGWAKVVSIASPLFTSIEESRALGEATRRAIDQSGERVALLAS
CCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
GSLSHRLWPNKNLGPEAWTSIASEFNRQVDLRVLQLWQERRYREFTAMLPDYAVKCNGEG
CCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCC
GMGDTVMLFAALGWDKYDGEAEPLCDYFPSSGSGQINVEFHVA
CCCHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCEEEEEEEEC
>Mature Secondary Structure 
GKLVLAAKVTHVPSLMLSEAGDSPLKQARAGAVESLRELGRRARTRDVSTFVVFDTHWL
CCEEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECHHH
SNFGYHVNANARHRGSFTSHEAPHMIQDLRYDLPGDTALAEAIASEAESAGLKVLAHQVP
HCCCEEEECCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHCC
TLGLEYGTIVPMHYLNPDGWAKVVSIASPLFTSIEESRALGEATRRAIDQSGERVALLAS
CCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
GSLSHRLWPNKNLGPEAWTSIASEFNRQVDLRVLQLWQERRYREFTAMLPDYAVKCNGEG
CCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCC
GMGDTVMLFAALGWDKYDGEAEPLCDYFPSSGSGQINVEFHVA
CCCHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2261999 [H]