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The map label for this gene is alkJ [H]

Identifier: 86750955

GI number: 86750955

Start: 4398972

End: 4400576

Strand: Direct

Name: alkJ [H]

Synonym: RPB_3847

Alternate gene names: 86750955

Gene position: 4398972-4400576 (Clockwise)

Preceding gene: 86750954

Following gene: 86750957

Centisome position: 82.51

GC content: 66.54

Gene sequence:

>1605_bases
ATGACGGACACCTATGACTTTGTCGTGGTCGGCGGCGGCTCGGGCGGCTGCGCGGTCGCAGGAAGGCTGTCGGAAGATCC
CGGCACGTCGGTGGCTTTGCTGGAAGCCGGCGGCACCGCCGACAATTGGGTGGTGAAGACACCCTATGCGCTGTCGCTGA
TGGTGCCGAGCAAACTCAACAACTGGCACTTCGAGACGGTGCCGCAGCCCGGCCTCAACGGCCGGATCGGCTATCAGCCG
CGCGGCAAGGGGCTCGGCGGATCGTCGGCGATCAACGCCATGGTCTACATTCGCGGCCATCAATGGGACTATGACCACTG
GGCCGAACTCGGCGCCACCGGCTGGTCCTATGCCGACGTGCTGCCGTATTTCAAACGTTCGGAGAGCAATTCCGATTTCA
ACGGCGCGTATCACGGCCAGAGCGGCCCGCTGCACGTCAACAAACTCCGCACCGACAATCCGGTGCACGAGATCTTCCTG
CAGGCGGCGCGCGAGGCGCAATTCCGCATTCGCGACGATTTCAACGGCGAGGAGCAGGAAGGCCTCGGCCTGTATCAGCT
CACCCAGCACAATGGCGAGCGCTGGAGCGCGGCCCGCGCCTATGTGCATCCCTACATGGCGACGCGGCCCAATCTGCGCG
TCGAGACGCAGGCGCAGGCCACCCGCATCCTGTTCGAGGGCGGTCGCGCGGTCGGCGTCGAATATCGCCAGAACGACGAA
GCCCGGCAGATCCGCGCGCGGCGCGAAGTCATCGTCGCGTCCGGCGCGTTCCAGTCGCCGCAACTGCTGATGCTGTCCGG
CATCGGCGACGCCGCCACGCTGCAGCAGCACGGCATCGCGCCGACGCATCATCTGCCCGGCGTCGGGCAGAATTTGCAGG
ATCACCCCGACTTCATCTTCGCCTATCAATCCGACAGCCCATATTTCACCGGCACCAGCTTCACCGGCATCGGCCGGTTG
CTGTCGCGGATCGGCCAGTACCGCCGCGAGGGCCGCGGCCCGCTCACGACCAACTTCGCCGAATGCGGCGGCTTCCTGAA
AACGCGACCGGACCTCGACGTGCCCGACGTGCAGTTGCATTTCGGCATGGCGATGGTCGACGACCACGGCCGCAAACGGC
GCTGGGGCACGGGCTTTTCCTGCCATGTCTGCCTGCTGCGGCCGAAAAGCCGCGGCAGCGTCGGCCTCGCAAGCGCCGAT
CCGCTGGCGCCGCCGCTGATCGACCCCAACTTTCTCGGCGAGGCGGACGATCTCGAGGCGATGGTCGCAGGCTACAAGAC
CACGCGGCGGTTGATGGAAGCCCCCGCGCTCCGCGCGCTGCAGCAGAAGGATCTGTTCACCGCCGACGTCCGCACCGATG
ACGACATCCGCGCCATCCTGCGCGCCCGCGTCGACACCGTGTATCACCCGGTCGGCACCTGCAGGATGGGCAGCGATCCG
ATGGCGGTGGTCGATCCGCAGCTTCGTGTGCACGGAATCGGCGGGCTGCGCATCGTCGACGCCTCGGTCATGCCGACCCT
GATCGGCGGCAACACCAACGCGCCGACGATCATGATCGGCGAGAAGGCCGCGGACATGATCCGGGAGGAGATCCGGGCGA
ACTGA

Upstream 100 bases:

>100_bases
GAAAGTGCTGCGCTGGATGTCCTGACCTGACGACGACCCGCCCGTCCAACGAGACGGCGCGGCCAAAAACAAGACCAACA
CCAAATATCCGGAGGGGACG

Downstream 100 bases:

>100_bases
ATCTTCACCGGGAGTCATTCCGGGTTCGCGCTAAGCGCGCCCCGGAATGACGAGCGAGAGGTGCTTGCCCACCTGAGCGC
GAACGACATCCAGAGCGGCC

Product: glucose-methanol-choline oxidoreductase

Products: Betaine Aldehyde; Reduced Acceptor. [C]

Alternate protein names: NA

Number of amino acids: Translated: 534; Mature: 533

Protein sequence:

>534_residues
MTDTYDFVVVGGGSGGCAVAGRLSEDPGTSVALLEAGGTADNWVVKTPYALSLMVPSKLNNWHFETVPQPGLNGRIGYQP
RGKGLGGSSAINAMVYIRGHQWDYDHWAELGATGWSYADVLPYFKRSESNSDFNGAYHGQSGPLHVNKLRTDNPVHEIFL
QAAREAQFRIRDDFNGEEQEGLGLYQLTQHNGERWSAARAYVHPYMATRPNLRVETQAQATRILFEGGRAVGVEYRQNDE
ARQIRARREVIVASGAFQSPQLLMLSGIGDAATLQQHGIAPTHHLPGVGQNLQDHPDFIFAYQSDSPYFTGTSFTGIGRL
LSRIGQYRREGRGPLTTNFAECGGFLKTRPDLDVPDVQLHFGMAMVDDHGRKRRWGTGFSCHVCLLRPKSRGSVGLASAD
PLAPPLIDPNFLGEADDLEAMVAGYKTTRRLMEAPALRALQQKDLFTADVRTDDDIRAILRARVDTVYHPVGTCRMGSDP
MAVVDPQLRVHGIGGLRIVDASVMPTLIGGNTNAPTIMIGEKAADMIREEIRAN

Sequences:

>Translated_534_residues
MTDTYDFVVVGGGSGGCAVAGRLSEDPGTSVALLEAGGTADNWVVKTPYALSLMVPSKLNNWHFETVPQPGLNGRIGYQP
RGKGLGGSSAINAMVYIRGHQWDYDHWAELGATGWSYADVLPYFKRSESNSDFNGAYHGQSGPLHVNKLRTDNPVHEIFL
QAAREAQFRIRDDFNGEEQEGLGLYQLTQHNGERWSAARAYVHPYMATRPNLRVETQAQATRILFEGGRAVGVEYRQNDE
ARQIRARREVIVASGAFQSPQLLMLSGIGDAATLQQHGIAPTHHLPGVGQNLQDHPDFIFAYQSDSPYFTGTSFTGIGRL
LSRIGQYRREGRGPLTTNFAECGGFLKTRPDLDVPDVQLHFGMAMVDDHGRKRRWGTGFSCHVCLLRPKSRGSVGLASAD
PLAPPLIDPNFLGEADDLEAMVAGYKTTRRLMEAPALRALQQKDLFTADVRTDDDIRAILRARVDTVYHPVGTCRMGSDP
MAVVDPQLRVHGIGGLRIVDASVMPTLIGGNTNAPTIMIGEKAADMIREEIRAN
>Mature_533_residues
TDTYDFVVVGGGSGGCAVAGRLSEDPGTSVALLEAGGTADNWVVKTPYALSLMVPSKLNNWHFETVPQPGLNGRIGYQPR
GKGLGGSSAINAMVYIRGHQWDYDHWAELGATGWSYADVLPYFKRSESNSDFNGAYHGQSGPLHVNKLRTDNPVHEIFLQ
AAREAQFRIRDDFNGEEQEGLGLYQLTQHNGERWSAARAYVHPYMATRPNLRVETQAQATRILFEGGRAVGVEYRQNDEA
RQIRARREVIVASGAFQSPQLLMLSGIGDAATLQQHGIAPTHHLPGVGQNLQDHPDFIFAYQSDSPYFTGTSFTGIGRLL
SRIGQYRREGRGPLTTNFAECGGFLKTRPDLDVPDVQLHFGMAMVDDHGRKRRWGTGFSCHVCLLRPKSRGSVGLASADP
LAPPLIDPNFLGEADDLEAMVAGYKTTRRLMEAPALRALQQKDLFTADVRTDDDIRAILRARVDTVYHPVGTCRMGSDPM
AVVDPQLRVHGIGGLRIVDASVMPTLIGGNTNAPTIMIGEKAADMIREEIRAN

Specific function: Converts aliphatic medium-chain-length alcohols into aldehydes. May be linked to the electron transfer chain [H]

COG id: COG2303

COG function: function code E; Choline dehydrogenase and related flavoproteins

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GMC oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI217272839, Length=539, Percent_Identity=43.9703153988868, Blast_Score=444, Evalue=1e-124,
Organism=Escherichia coli, GI1786503, Length=533, Percent_Identity=40.7129455909944, Blast_Score=382, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI17532301, Length=545, Percent_Identity=39.0825688073394, Blast_Score=403, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24642042, Length=584, Percent_Identity=38.6986301369863, Blast_Score=335, Evalue=4e-92,
Organism=Drosophila melanogaster, GI24642048, Length=569, Percent_Identity=38.1370826010545, Blast_Score=335, Evalue=6e-92,
Organism=Drosophila melanogaster, GI24642055, Length=576, Percent_Identity=37.3263888888889, Blast_Score=332, Evalue=3e-91,
Organism=Drosophila melanogaster, GI45549471, Length=569, Percent_Identity=37.9613356766257, Blast_Score=329, Evalue=3e-90,
Organism=Drosophila melanogaster, GI45551458, Length=569, Percent_Identity=37.9613356766257, Blast_Score=329, Evalue=3e-90,
Organism=Drosophila melanogaster, GI24642035, Length=575, Percent_Identity=38.2608695652174, Blast_Score=320, Evalue=2e-87,
Organism=Drosophila melanogaster, GI24642037, Length=576, Percent_Identity=37.6736111111111, Blast_Score=320, Evalue=2e-87,
Organism=Drosophila melanogaster, GI24642039, Length=570, Percent_Identity=36.8421052631579, Blast_Score=313, Evalue=2e-85,
Organism=Drosophila melanogaster, GI24642059, Length=576, Percent_Identity=36.1111111111111, Blast_Score=312, Evalue=4e-85,
Organism=Drosophila melanogaster, GI24650267, Length=571, Percent_Identity=36.077057793345, Blast_Score=312, Evalue=4e-85,
Organism=Drosophila melanogaster, GI18859995, Length=567, Percent_Identity=36.8606701940035, Blast_Score=311, Evalue=5e-85,
Organism=Drosophila melanogaster, GI17137792, Length=553, Percent_Identity=37.251356238698, Blast_Score=305, Evalue=4e-83,
Organism=Drosophila melanogaster, GI18859993, Length=569, Percent_Identity=33.2161687170475, Blast_Score=274, Evalue=1e-73,
Organism=Drosophila melanogaster, GI24642051, Length=574, Percent_Identity=32.2299651567944, Blast_Score=265, Evalue=8e-71,
Organism=Drosophila melanogaster, GI24645930, Length=586, Percent_Identity=31.0580204778157, Blast_Score=192, Evalue=7e-49,
Organism=Drosophila melanogaster, GI24642057, Length=584, Percent_Identity=30.3082191780822, Blast_Score=157, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012132
- InterPro:   IPR000172
- InterPro:   IPR007867 [H]

Pfam domain/function: PF05199 GMC_oxred_C; PF00732 GMC_oxred_N [H]

EC number: 1.1.99.1 [C]

Molecular weight: Translated: 58496; Mature: 58364

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: PS00623 GMC_OXRED_1 ; PS00624 GMC_OXRED_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDTYDFVVVGGGSGGCAVAGRLSEDPGTSVALLEAGGTADNWVVKTPYALSLMVPSKLN
CCCCEEEEEEECCCCCEEEEEECCCCCCCEEEEEECCCCCCCCEEECCEEEEEEECCCCC
NWHFETVPQPGLNGRIGYQPRGKGLGGSSAINAMVYIRGHQWDYDHWAELGATGWSYADV
CCEEEECCCCCCCCEECCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHCCCCCCHHHH
LPYFKRSESNSDFNGAYHGQSGPLHVNKLRTDNPVHEIFLQAAREAQFRIRDDFNGEEQE
HHHHHCCCCCCCCCCEECCCCCCEEEEEECCCCHHHHHHHHHHHHCCEEEECCCCCCHHH
GLGLYQLTQHNGERWSAARAYVHPYMATRPNLRVETQAQATRILFEGGRAVGVEYRQNDE
CCCEEEEECCCCCCHHHHHHHHCHHHCCCCCCEEECHHHHEEEEEECCCEEEEEECCCCH
ARQIRARREVIVASGAFQSPQLLMLSGIGDAATLQQHGIAPTHHLPGVGQNLQDHPDFIF
HHHHHHHHEEEEECCCCCCCCEEEEECCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCEEE
AYQSDSPYFTGTSFTGIGRLLSRIGQYRREGRGPLTTNFAECGGFLKTRPDLDVPDVQLH
EEECCCCEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCCCCCCEEEE
FGMAMVDDHGRKRRWGTGFSCHVCLLRPKSRGSVGLASADPLAPPLIDPNFLGEADDLEA
ECEEEECCCCCCCCCCCCCEEEEEEECCCCCCCEEECCCCCCCCCCCCCCCCCCCHHHHH
MVAGYKTTRRLMEAPALRALQQKDLFTADVRTDDDIRAILRARVDTVYHPVGTCRMGSDP
HHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCEEECCCC
MAVVDPQLRVHGIGGLRIVDASVMPTLIGGNTNAPTIMIGEKAADMIREEIRAN
CEEECCCEEEEECCCEEEEECCCCCEEECCCCCCCEEEECHHHHHHHHHHHHCC
>Mature Secondary Structure 
TDTYDFVVVGGGSGGCAVAGRLSEDPGTSVALLEAGGTADNWVVKTPYALSLMVPSKLN
CCCEEEEEEECCCCCEEEEEECCCCCCCEEEEEECCCCCCCCEEECCEEEEEEECCCCC
NWHFETVPQPGLNGRIGYQPRGKGLGGSSAINAMVYIRGHQWDYDHWAELGATGWSYADV
CCEEEECCCCCCCCEECCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHCCCCCCHHHH
LPYFKRSESNSDFNGAYHGQSGPLHVNKLRTDNPVHEIFLQAAREAQFRIRDDFNGEEQE
HHHHHCCCCCCCCCCEECCCCCCEEEEEECCCCHHHHHHHHHHHHCCEEEECCCCCCHHH
GLGLYQLTQHNGERWSAARAYVHPYMATRPNLRVETQAQATRILFEGGRAVGVEYRQNDE
CCCEEEEECCCCCCHHHHHHHHCHHHCCCCCCEEECHHHHEEEEEECCCEEEEEECCCCH
ARQIRARREVIVASGAFQSPQLLMLSGIGDAATLQQHGIAPTHHLPGVGQNLQDHPDFIF
HHHHHHHHEEEEECCCCCCCCEEEEECCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCEEE
AYQSDSPYFTGTSFTGIGRLLSRIGQYRREGRGPLTTNFAECGGFLKTRPDLDVPDVQLH
EEECCCCEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCCCCCCEEEE
FGMAMVDDHGRKRRWGTGFSCHVCLLRPKSRGSVGLASADPLAPPLIDPNFLGEADDLEA
ECEEEECCCCCCCCCCCCCEEEEEEECCCCCCCEEECCCCCCCCCCCCCCCCCCCHHHHH
MVAGYKTTRRLMEAPALRALQQKDLFTADVRTDDDIRAILRARVDTVYHPVGTCRMGSDP
HHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCEEECCCC
MAVVDPQLRVHGIGGLRIVDASVMPTLIGGNTNAPTIMIGEKAADMIREEIRAN
CEEECCCEEEEECCCEEEEECCCCCEEECCCCCCCEEEECHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Coenzyme Q [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 7 {choline}} 1.1 {phenazine}} [C]

Substrates: Choline; Acceptor [C]

Specific reaction: Choline + Acceptor = Betaine Aldehyde + Reduced Acceptor. [C]

General reaction: Redox reaction [C]

Inhibitor: 2-Dimethylaminoethanol; Betaine aldehyde; Monoethanolamine; Semicarbazide [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11390693 [H]