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The map label for this gene is gcvH [H]

Identifier: 86750848

GI number: 86750848

Start: 4277866

End: 4278231

Strand: Direct

Name: gcvH [H]

Synonym: RPB_3739

Alternate gene names: 86750848

Gene position: 4277866-4278231 (Clockwise)

Preceding gene: 86750847

Following gene: 86750849

Centisome position: 80.24

GC content: 63.11

Gene sequence:

>366_bases
ATGACCACGCTCTACACCACCGATCACGAATGGCTGAAAATCGACGGCGACGTCGCCACGATCGGCATCACCAACTACGC
GCAGGAACAGCTCGGCGACGTGGTGTTCGTCGAACTGCCGAAGGTCGGCCGTAGCGTCAAGAAGGCGGAAGCCGCAGCCG
TGGTCGAATCGGTCAAGGCCGCCTCGGATGTCTACGCCCCGATCACCGGCGAAGTGCTCGAGGTCAACGAGACGCTCGGC
ACCGAACCTGCATTGGTGAATTCCGACGCCGGCGGCGCGGCGTGGTTCTTCAAGCTGAAGATCGCCGACAAAAGCGAACT
GGACGGCCTGATGGACGAAGCCGCCTACGCGGCGCACACGGCCTGA

Upstream 100 bases:

>100_bases
CGACAGCCTGGTTTTCGCCGAAGTGCGCGGCCAGCGGCTGCCGCTGCGCGTCGCGGCCATGCCTTTCGTAACCAACACCT
ACAAGCGCTGAGGGATGCTC

Downstream 100 bases:

>100_bases
ACCCAAGCCGTCATGCCCGGGCTTGTCCCGGCATCCACGCCCTTGGCGTATCGATGGCTGCAAGACGTGGATGGCCGGGA
CGAGCCCGGCCATGACGGAA

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 121; Mature: 120

Protein sequence:

>121_residues
MTTLYTTDHEWLKIDGDVATIGITNYAQEQLGDVVFVELPKVGRSVKKAEAAAVVESVKAASDVYAPITGEVLEVNETLG
TEPALVNSDAGGAAWFFKLKIADKSELDGLMDEAAYAAHTA

Sequences:

>Translated_121_residues
MTTLYTTDHEWLKIDGDVATIGITNYAQEQLGDVVFVELPKVGRSVKKAEAAAVVESVKAASDVYAPITGEVLEVNETLG
TEPALVNSDAGGAAWFFKLKIADKSELDGLMDEAAYAAHTA
>Mature_120_residues
TTLYTTDHEWLKIDGDVATIGITNYAQEQLGDVVFVELPKVGRSVKKAEAAAVVESVKAASDVYAPITGEVLEVNETLGT
EPALVNSDAGGAAWFFKLKIADKSELDGLMDEAAYAAHTA

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI89057342, Length=115, Percent_Identity=45.2173913043478, Blast_Score=119, Evalue=8e-28,
Organism=Homo sapiens, GI49574537, Length=115, Percent_Identity=45.2173913043478, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1789271, Length=115, Percent_Identity=52.1739130434783, Blast_Score=125, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI17551294, Length=113, Percent_Identity=46.0176991150442, Blast_Score=120, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17507493, Length=114, Percent_Identity=48.2456140350877, Blast_Score=117, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6319272, Length=116, Percent_Identity=40.5172413793103, Blast_Score=97, Evalue=6e-22,
Organism=Drosophila melanogaster, GI17865652, Length=115, Percent_Identity=46.0869565217391, Blast_Score=110, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 12845; Mature: 12714

Theoretical pI: Translated: 4.12; Mature: 4.12

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTLYTTDHEWLKIDGDVATIGITNYAQEQLGDVVFVELPKVGRSVKKAEAAAVVESVKA
CCEEEECCCCEEEECCCEEEEEECHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHH
ASDVYAPITGEVLEVNETLGTEPALVNSDAGGAAWFFKLKIADKSELDGLMDEAAYAAHT
HHHCCCCCCCCEEEECCCCCCCCCEEECCCCCEEEEEEEEECCCHHHHHHHHHHHHHHCC
A
C
>Mature Secondary Structure 
TTLYTTDHEWLKIDGDVATIGITNYAQEQLGDVVFVELPKVGRSVKKAEAAAVVESVKA
CEEEECCCCEEEECCCEEEEEECHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHH
ASDVYAPITGEVLEVNETLGTEPALVNSDAGGAAWFFKLKIADKSELDGLMDEAAYAAHT
HHHCCCCCCCCEEEECCCCCCCCCEEECCCCCEEEEEEEEECCCHHHHHHHHHHHHHHCC
A
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]