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The map label for this gene is livJ [C]

Identifier: 86750795

GI number: 86750795

Start: 4224793

End: 4225908

Strand: Direct

Name: livJ [C]

Synonym: RPB_3685

Alternate gene names: 86750795

Gene position: 4224793-4225908 (Clockwise)

Preceding gene: 86750794

Following gene: 86750797

Centisome position: 79.24

GC content: 62.99

Gene sequence:

>1116_bases
ATGAAACTCAAGCTTCTCGGTTTGGCATTCGGCGCGTCGCTGGCGCTGTCGACCACGGCGCTGGCACAGGACATCAAGGT
CGCGGTCTCGGGGCCGATGACCGGCAGCGAATCCGCGTTCGGACGGCAGTTGAAGAACGGCGCCGATCAGGCGGTCGCCG
ATCTCAACGCCGGCGGCGGCGTGCTCGGCAAGAAGCTGGCGCTGCAGATCGGCGACGACGCCTGCGATCCCAAGCAGGCA
CGCTCTATCGCCGAGAAACTGGCAGGCGAAGGCATCCCGTTCGTCGCCGGGCATTTCTGTTCGTCGTCGTCGATCCCGGC
GTCGGAAGCCTATGCGGACGGCAACGTGCTGCAGATCACGCCCGCCTCGACCAACCCGCTGTTCACCGAGCGCAAGCTGT
GGAACGTGCTGCGCGTCTGCGGCCGCGACGATCAGCAGGGCCTGATCGCCGCCGAGTACATCACCAAGAATTTCAAGGGC
AAGAACGTCGCCATCCTCAACGACAAGACCACCTACGGCAAGGGTCTCGCCGATGAAACCAAGAAGGCGCTGAACAAGGC
CGGCTTCCAGGAGAAGATGTTCGAGTCCTACAACAAGGGCGACAAGGACTTCAATTCGATCGTCTCGCGGCTGAAGCGCG
ACTCGATCGATCTCGTCTATATCGGCGGCTACCATCAGGAAGCCGGGCTGATCCTGCGGCAGATGCGCGACCAGGGCCTG
AAGACCGCGATGATGGCCGGCGACGCGATGAACGACAAGGAATTCGCCTCGATCACCGGCCCGCTCGCCGAGGGCACGCT
GTTCACCTTCGGCCCCGACCCGCGCAACAAGCCGACCGCCAAGGCGATCGTCGAGAAGTTCAAGGCCAAGGGCATCGATC
CGGAAGGCTACACGCTCTACACCTACGCCGCGTTCCAGGTCTGGTCGCAGGCCGTCGCCAAGGCCAAGACCACCGATCCG
AAGAAGGTGATCGACACCATCAAGGCCGGCGAATGGGACACCGTGCTCGGCAAGATGGCGTTCGACGCCAAGGGCGACAT
CAAGGCGATCGACTACGTCGTCTACAAATGGGACGCCAAGGGCAACTACGCGGAAATCCCCGGCAAGGCGATGTGA

Upstream 100 bases:

>100_bases
ATGACTTGGCCGGAAAATCAGCCAACAATGCCACCAGGCTTCGCGCCTCGGCACGGCTCGGGCGCGCTGTTCGTTCGCAC
ATTACCGCAGGGATCGATCC

Downstream 100 bases:

>100_bases
GGCGTTGGGCTTTCGATCGTCATTGCGAGGAGCGAAGCGACGAAGCAATCCAAACAGTGTTTGCTGCTCTGGATTGCTTC
GCTACGTTCGCAATGACGTT

Product: extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MKLKLLGLAFGASLALSTTALAQDIKVAVSGPMTGSESAFGRQLKNGADQAVADLNAGGGVLGKKLALQIGDDACDPKQA
RSIAEKLAGEGIPFVAGHFCSSSSIPASEAYADGNVLQITPASTNPLFTERKLWNVLRVCGRDDQQGLIAAEYITKNFKG
KNVAILNDKTTYGKGLADETKKALNKAGFQEKMFESYNKGDKDFNSIVSRLKRDSIDLVYIGGYHQEAGLILRQMRDQGL
KTAMMAGDAMNDKEFASITGPLAEGTLFTFGPDPRNKPTAKAIVEKFKAKGIDPEGYTLYTYAAFQVWSQAVAKAKTTDP
KKVIDTIKAGEWDTVLGKMAFDAKGDIKAIDYVVYKWDAKGNYAEIPGKAM

Sequences:

>Translated_371_residues
MKLKLLGLAFGASLALSTTALAQDIKVAVSGPMTGSESAFGRQLKNGADQAVADLNAGGGVLGKKLALQIGDDACDPKQA
RSIAEKLAGEGIPFVAGHFCSSSSIPASEAYADGNVLQITPASTNPLFTERKLWNVLRVCGRDDQQGLIAAEYITKNFKG
KNVAILNDKTTYGKGLADETKKALNKAGFQEKMFESYNKGDKDFNSIVSRLKRDSIDLVYIGGYHQEAGLILRQMRDQGL
KTAMMAGDAMNDKEFASITGPLAEGTLFTFGPDPRNKPTAKAIVEKFKAKGIDPEGYTLYTYAAFQVWSQAVAKAKTTDP
KKVIDTIKAGEWDTVLGKMAFDAKGDIKAIDYVVYKWDAKGNYAEIPGKAM
>Mature_371_residues
MKLKLLGLAFGASLALSTTALAQDIKVAVSGPMTGSESAFGRQLKNGADQAVADLNAGGGVLGKKLALQIGDDACDPKQA
RSIAEKLAGEGIPFVAGHFCSSSSIPASEAYADGNVLQITPASTNPLFTERKLWNVLRVCGRDDQQGLIAAEYITKNFKG
KNVAILNDKTTYGKGLADETKKALNKAGFQEKMFESYNKGDKDFNSIVSRLKRDSIDLVYIGGYHQEAGLILRQMRDQGL
KTAMMAGDAMNDKEFASITGPLAEGTLFTFGPDPRNKPTAKAIVEKFKAKGIDPEGYTLYTYAAFQVWSQAVAKAKTTDP
KKVIDTIKAGEWDTVLGKMAFDAKGDIKAIDYVVYKWDAKGNYAEIPGKAM

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI48994941, Length=355, Percent_Identity=44.5070422535211, Blast_Score=286, Evalue=2e-78,
Organism=Escherichia coli, GI1789867, Length=351, Percent_Identity=41.8803418803419, Blast_Score=281, Evalue=4e-77,

Paralogues:

None

Copy number: 10140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 8822 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal med

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 39850; Mature: 39850

Theoretical pI: Translated: 9.26; Mature: 9.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKLLGLAFGASLALSTTALAQDIKVAVSGPMTGSESAFGRQLKNGADQAVADLNAGGG
CEEEEEEEHHCCHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHCCHHHHHHHCCCCCC
VLGKKLALQIGDDACDPKQARSIAEKLAGEGIPFVAGHFCSSSSIPASEAYADGNVLQIT
HHCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCEEEHHCCCCCCCCCHHHCCCCCEEEEE
PASTNPLFTERKLWNVLRVCGRDDQQGLIAAEYITKNFKGKNVAILNDKTTYGKGLADET
CCCCCCCHHHHHHHHHHHHHCCCCCCCEEEHHHHHHCCCCCEEEEEECCCCCCCCCHHHH
KKALNKAGFQEKMFESYNKGDKDFNSIVSRLKRDSIDLVYIGGYHQEAGLILRQMRDQGL
HHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHCCH
KTAMMAGDAMNDKEFASITGPLAEGTLFTFGPDPRNKPTAKAIVEKFKAKGIDPEGYTLY
HHHHHCCCCCCCCHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCEEEE
TYAAFQVWSQAVAKAKTTDPKKVIDTIKAGEWDTVLGKMAFDAKGDIKAIDYVVYKWDAK
EHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCCEEEEEEEEEECCC
GNYAEIPGKAM
CCEECCCCCCC
>Mature Secondary Structure
MKLKLLGLAFGASLALSTTALAQDIKVAVSGPMTGSESAFGRQLKNGADQAVADLNAGGG
CEEEEEEEHHCCHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHCCHHHHHHHCCCCCC
VLGKKLALQIGDDACDPKQARSIAEKLAGEGIPFVAGHFCSSSSIPASEAYADGNVLQIT
HHCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCEEEHHCCCCCCCCCHHHCCCCCEEEEE
PASTNPLFTERKLWNVLRVCGRDDQQGLIAAEYITKNFKGKNVAILNDKTTYGKGLADET
CCCCCCCHHHHHHHHHHHHHCCCCCCCEEEHHHHHHCCCCCEEEEEECCCCCCCCCHHHH
KKALNKAGFQEKMFESYNKGDKDFNSIVSRLKRDSIDLVYIGGYHQEAGLILRQMRDQGL
HHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHCCH
KTAMMAGDAMNDKEFASITGPLAEGTLFTFGPDPRNKPTAKAIVEKFKAKGIDPEGYTLY
HHHHHCCCCCCCCHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCEEEE
TYAAFQVWSQAVAKAKTTDPKKVIDTIKAGEWDTVLGKMAFDAKGDIKAIDYVVYKWDAK
EHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCCEEEEEEEEEECCC
GNYAEIPGKAM
CCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA