Click here to switch to the map view.

The map label for this gene is 86750739

Identifier: 86750739

GI number: 86750739

Start: 4165521

End: 4166954

Strand: Direct

Name: 86750739

Synonym: RPB_3629

Alternate gene names: NA

Gene position: 4165521-4166954 (Clockwise)

Preceding gene: 86750737

Following gene: 86750740

Centisome position: 78.13

GC content: 69.53

Gene sequence:

>1434_bases
ATGGCGGCACACCACGAGCTGATTCAAGCGACGGCCTGCAAGATCGTCGACCAACTCCGAACCGGCGAGGTCACGCCGCT
CGATCTGCTCGACGCGCTGGAGCAGCGGATCGCCGAGGTCGACGGCGCCGTCAACGCGCTGCCGACGCTGTGCTTCGATC
GCGCCCGCGCCCGTGCCGCCGACCTGATGAAGAAGCCGCAGTCCGAACGCGGCCTGCTCGCCGGCCTTCCGGTGCCGATC
AAGGACCTCACCGCGGTCGAAGGCGTGCGGACGACCCAGGGGTCGCCGATCTTCAAGGACCATGTCCCTACCCGCTCCGA
CCTGATGGTCGAGCACCTCGAAGCCAGTGGCGGGGTGGTCTACGCCAAATCCAACACCCCGGAATTCGGCGCCGGCGCCA
ACACCTTCAACGAGGTGTTCGGGGCGACGCGCAATCCGTGGGATTTGTCGCGCTCGGCGGCAGGCTCGTCCGGCGGCGCT
GCGGCCGCGCTCGCCAGCGGCACCGCGTGGCTCGCCCACGGCTCGGACATGGGCGGCTCGCTGCGCAATCCGGCGAGCTT
CTGCGGCGTCGTCGGCCTGCGGCCGAGCTTCGGCCGTGTCGCGCACACGCCCGCAGCAGCGATCGACCGCAATCTCGGCG
TGCAGGGCCCGATGGCGCGCAATGTCGAGGACGTCGCCCTGTTGCTCGACGCGATGAGCGGCGAACACGCCGCCGATCCG
CTGTCGCTGCCCTCGCCGGTAACCTCGTTCCTCGCCGCCGCGCGCGCGCGCACCAAGCCGCTGCGCGTGGCTTATTCGCC
CGACCTCGGCATCACCCCGGTCGATCCCGAGGTCGCCGCGATCACCCGCAAGGCGGCCGAGCGCTTCGCCGAATCCGGCG
TGGTCGTAGAGCAGGCTCAGCCCGACCTCTCTGAAGCGCACGAATGCTTCCACGTGCTGCGCGCATTCGACTTCGCGATC
AGCAAGGCGGAACTGCTCCGCACCAAGCGTGATCTGCTGAAGCCGGAGGTGATCTGGAACATCGAGCAGGGGCTCGCGCT
CAGCGTCGACGATCTGGCCCGGGCCGAGGCGCAGCGCGTGGCGATGGCCGCACGCACCATCGCGTTCTTCGAGACTTACG
ATCTGCTGCTGTGTCCGGCGACGATCACCGCGCCTTTCCCCGTGGAGCAGCGCTACCTCGCAGAATGCAACGGCCACACC
TTCGACAACTACGTCGAGTGGCTCGGCATCGTCTACGCGATCACGCTGGTCTGCTGCCCGGCGCTGTCGCTGCCCTGCGG
CTTCACGTCGGCCGGCCTGCCGGTCGGGCTGCAGATGATTGCTCGGCCGCGCAACGAGGCCGGCCTGCTCGCCGGCGCCG
CATTGCTCGAAGACGTTCTCGGCCTGCGCGGCACCACGCCGATCGATCCGCGGCCCGCGCCAGCGAGCCTCTGA

Upstream 100 bases:

>100_bases
AAGCAGGTCGGGCTACCCATTGCGTCGTAGCCGGACGGCATAATCGCCCGCCTGCCTTGCACCCGCACGGCCGGCCGATC
AAGTCAGGAGAGACAACAGG

Downstream 100 bases:

>100_bases
GCCTTCCAAAGGACACTGGCGGACGTGACGCGCTGTGCTAGTCTCGGCGCGTCACGACACTCACAGATCCGCCGCCAAGG
CGGACGACGAGGAAACGCCG

Product: amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 477; Mature: 476

Protein sequence:

>477_residues
MAAHHELIQATACKIVDQLRTGEVTPLDLLDALEQRIAEVDGAVNALPTLCFDRARARAADLMKKPQSERGLLAGLPVPI
KDLTAVEGVRTTQGSPIFKDHVPTRSDLMVEHLEASGGVVYAKSNTPEFGAGANTFNEVFGATRNPWDLSRSAAGSSGGA
AAALASGTAWLAHGSDMGGSLRNPASFCGVVGLRPSFGRVAHTPAAAIDRNLGVQGPMARNVEDVALLLDAMSGEHAADP
LSLPSPVTSFLAAARARTKPLRVAYSPDLGITPVDPEVAAITRKAAERFAESGVVVEQAQPDLSEAHECFHVLRAFDFAI
SKAELLRTKRDLLKPEVIWNIEQGLALSVDDLARAEAQRVAMAARTIAFFETYDLLLCPATITAPFPVEQRYLAECNGHT
FDNYVEWLGIVYAITLVCCPALSLPCGFTSAGLPVGLQMIARPRNEAGLLAGAALLEDVLGLRGTTPIDPRPAPASL

Sequences:

>Translated_477_residues
MAAHHELIQATACKIVDQLRTGEVTPLDLLDALEQRIAEVDGAVNALPTLCFDRARARAADLMKKPQSERGLLAGLPVPI
KDLTAVEGVRTTQGSPIFKDHVPTRSDLMVEHLEASGGVVYAKSNTPEFGAGANTFNEVFGATRNPWDLSRSAAGSSGGA
AAALASGTAWLAHGSDMGGSLRNPASFCGVVGLRPSFGRVAHTPAAAIDRNLGVQGPMARNVEDVALLLDAMSGEHAADP
LSLPSPVTSFLAAARARTKPLRVAYSPDLGITPVDPEVAAITRKAAERFAESGVVVEQAQPDLSEAHECFHVLRAFDFAI
SKAELLRTKRDLLKPEVIWNIEQGLALSVDDLARAEAQRVAMAARTIAFFETYDLLLCPATITAPFPVEQRYLAECNGHT
FDNYVEWLGIVYAITLVCCPALSLPCGFTSAGLPVGLQMIARPRNEAGLLAGAALLEDVLGLRGTTPIDPRPAPASL
>Mature_476_residues
AAHHELIQATACKIVDQLRTGEVTPLDLLDALEQRIAEVDGAVNALPTLCFDRARARAADLMKKPQSERGLLAGLPVPIK
DLTAVEGVRTTQGSPIFKDHVPTRSDLMVEHLEASGGVVYAKSNTPEFGAGANTFNEVFGATRNPWDLSRSAAGSSGGAA
AALASGTAWLAHGSDMGGSLRNPASFCGVVGLRPSFGRVAHTPAAAIDRNLGVQGPMARNVEDVALLLDAMSGEHAADPL
SLPSPVTSFLAAARARTKPLRVAYSPDLGITPVDPEVAAITRKAAERFAESGVVVEQAQPDLSEAHECFHVLRAFDFAIS
KAELLRTKRDLLKPEVIWNIEQGLALSVDDLARAEAQRVAMAARTIAFFETYDLLLCPATITAPFPVEQRYLAECNGHTF
DNYVEWLGIVYAITLVCCPALSLPCGFTSAGLPVGLQMIARPRNEAGLLAGAALLEDVLGLRGTTPIDPRPAPASL

Specific function: Unknown

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=488, Percent_Identity=25.4098360655738, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI195972892, Length=241, Percent_Identity=29.4605809128631, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI166795287, Length=257, Percent_Identity=33.852140077821, Blast_Score=89, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17537465, Length=504, Percent_Identity=28.3730158730159, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17538252, Length=343, Percent_Identity=27.6967930029155, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI71990152, Length=300, Percent_Identity=28.6666666666667, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17556264, Length=287, Percent_Identity=28.5714285714286, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17543272, Length=448, Percent_Identity=25, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17538254, Length=419, Percent_Identity=25.5369928400955, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17556276, Length=321, Percent_Identity=29.2834890965732, Blast_Score=96, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17556278, Length=321, Percent_Identity=29.2834890965732, Blast_Score=95, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6320448, Length=241, Percent_Identity=30.2904564315353, Blast_Score=90, Evalue=8e-19,
Organism=Saccharomyces cerevisiae, GI6319685, Length=439, Percent_Identity=25.2847380410023, Blast_Score=77, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6323950, Length=195, Percent_Identity=27.6923076923077, Blast_Score=68, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24644968, Length=502, Percent_Identity=26.8924302788845, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24648113, Length=499, Percent_Identity=28.6573146292585, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI45550774, Length=258, Percent_Identity=33.7209302325581, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24648435, Length=258, Percent_Identity=33.3333333333333, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI24648437, Length=258, Percent_Identity=33.3333333333333, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI21356731, Length=488, Percent_Identity=27.2540983606557, Blast_Score=121, Evalue=8e-28,
Organism=Drosophila melanogaster, GI24648441, Length=416, Percent_Identity=27.4038461538462, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24648439, Length=416, Percent_Identity=27.4038461538462, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI161078093, Length=508, Percent_Identity=26.9685039370079, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24652985, Length=248, Percent_Identity=31.0483870967742, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI19922090, Length=248, Percent_Identity=31.0483870967742, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI24652981, Length=248, Percent_Identity=31.0483870967742, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI24652983, Length=248, Percent_Identity=31.0483870967742, Blast_Score=99, Evalue=7e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: =3.5.1.4 [H]

Molecular weight: Translated: 50489; Mature: 50358

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAHHELIQATACKIVDQLRTGEVTPLDLLDALEQRIAEVDGAVNALPTLCFDRARARAA
CCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLMKKPQSERGLLAGLPVPIKDLTAVEGVRTTQGSPIFKDHVPTRSDLMVEHLEASGGVV
HHHHCCCCCCCEEECCCCCHHHHHHHCCCCCCCCCCCHHCCCCCHHHHHHHHHHCCCCEE
YAKSNTPEFGAGANTFNEVFGATRNPWDLSRSAAGSSGGAAAALASGTAWLAHGSDMGGS
EECCCCCCCCCCCHHHHHHHCCCCCCCCCHHHCCCCCCCCHHHHHCCCEEEECCCCCCCC
LRNPASFCGVVGLRPSFGRVAHTPAAAIDRNLGVQGPMARNVEDVALLLDAMSGEHAADP
CCCCHHHHHHCCCCCCCCCHHCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCC
LSLPSPVTSFLAAARARTKPLRVAYSPDLGITPVDPEVAAITRKAAERFAESGVVVEQAQ
CCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEECCC
PDLSEAHECFHVLRAFDFAISKAELLRTKRDLLKPEVIWNIEQGLALSVDDLARAEAQRV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEECCCCCEECHHHHHHHHHHHH
AMAARTIAFFETYDLLLCPATITAPFPVEQRYLAECNGHTFDNYVEWLGIVYAITLVCCP
HHHHHHHHHHHHHHHEEECCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
ALSLPCGFTSAGLPVGLQMIARPRNEAGLLAGAALLEDVLGLRGTTPIDPRPAPASL
HHCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AAHHELIQATACKIVDQLRTGEVTPLDLLDALEQRIAEVDGAVNALPTLCFDRARARAA
CCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLMKKPQSERGLLAGLPVPIKDLTAVEGVRTTQGSPIFKDHVPTRSDLMVEHLEASGGVV
HHHHCCCCCCCEEECCCCCHHHHHHHCCCCCCCCCCCHHCCCCCHHHHHHHHHHCCCCEE
YAKSNTPEFGAGANTFNEVFGATRNPWDLSRSAAGSSGGAAAALASGTAWLAHGSDMGGS
EECCCCCCCCCCCHHHHHHHCCCCCCCCCHHHCCCCCCCCHHHHHCCCEEEECCCCCCCC
LRNPASFCGVVGLRPSFGRVAHTPAAAIDRNLGVQGPMARNVEDVALLLDAMSGEHAADP
CCCCHHHHHHCCCCCCCCCHHCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCC
LSLPSPVTSFLAAARARTKPLRVAYSPDLGITPVDPEVAAITRKAAERFAESGVVVEQAQ
CCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEECCC
PDLSEAHECFHVLRAFDFAISKAELLRTKRDLLKPEVIWNIEQGLALSVDDLARAEAQRV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEECCCCCEECHHHHHHHHHHHH
AMAARTIAFFETYDLLLCPATITAPFPVEQRYLAECNGHTFDNYVEWLGIVYAITLVCCP
HHHHHHHHHHHHHHHEEECCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
ALSLPCGFTSAGLPVGLQMIARPRNEAGLLAGAALLEDVLGLRGTTPIDPRPAPASL
HHCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]