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The map label for this gene is pleD [H]

Identifier: 86750660

GI number: 86750660

Start: 4063224

End: 4064369

Strand: Direct

Name: pleD [H]

Synonym: RPB_3550

Alternate gene names: 86750660

Gene position: 4063224-4064369 (Clockwise)

Preceding gene: 86750654

Following gene: 86750661

Centisome position: 76.21

GC content: 63.61

Gene sequence:

>1146_bases
ATGGGATCAGCTGCCGTCATTTCGGGCATGCCAGCGGGCACGGTGGACGGGGCCACACGGGCTCGGACGCTTGCTGTCCG
TGCGCGGAGAATGAAGCAACGCCGGCAGATGTTCGATCTGGTCGCAGCGAGCTATCTGACCGACGCCCTGCTCCTGCTGA
TCTACAGCTTCACCGGCACGATCCCCGAGATCGTCGTTCCGACCTACATCGCCTGCGGCTTCGGCATCATCGTCGGAAGC
ATTGCGCTGTCGGAATCGGGTTTTTACGATCGTTTCAAGGACCACTATCTGGTTCTGCCGACCGTCGCGCTCAATGTCGC
GCTGGTGATGGGATTTTGCATCTGGGTGCCGCAGATCGCATTCCTGTTCCTGTGCTCGCTGTTCATCATCTTCAATTTCG
CGTCGCTGCGCGCCAGCACAAGTCAGAGCTTGATCTGCTGGGCGACGATGGCTGCCGCGTTGGTGGTGCTGTTGGTCTTC
ACGGACACGCCGATCGCGATTCCGCACGACACTCTGCTCGAGCGCACCGTGACGATGCTGGCGCTGGTGCTGACCCTCGG
CCGCTGCATGTTCATCGGGATGTATTCCAGCGCGATGCGGGTGTCGCTCTACAAACGCGGTCTCCAGTTGCGCGAGGCCT
ACCGGCGGATCGAGGAGCTCGCGGAGCTCGACGAACTCACCGGCGCGTTCAATCGCCGCTGTATCATGCGGATGCTCGAC
GACGAGATCGCCCGGGCCGAACGCAGCGCGACGCCGCTGTCGCTCGCCTTGATCGACCTCGACTGGTTCAAGCGGATCAA
CGACAGCTTCGGCCACCCCACCGGCGACGAGGTCCTGCGCACCTTCGCGATCACCATCTTCGCCAACATCCGCAGCTTCG
ACCGGTTCGGCCGCTACGGCGGCGAGGAATTCCTGCTGCTGCTGCCGAACACGTCGCGCGAAGACGCCCACGCCCTGCTG
GACCGGCTGCGGGAAATCGTCGGGGAGCTGGACTGGACGGCGTTCTCGCCCGGGATGCGGGTGACGCTCTCCGCCGGTGT
CACGACGATGTCTCCGGACGAGACTGCCGAATCCGTGCTGGCCCGCGCCGACAGTGCGCTCTATGCGGCCAAGGAAAAGG
GACGCAACAGAATCGCCACGACCTGA

Upstream 100 bases:

>100_bases
GTCATATACTTAATCGGAGCGAGCGGCGGGTTCCCCGCAGATACGAAACCTAAAGGCTTTGCTCGTAAGTTCCCTCAACT
CAAAGGCGTTGAAGCGGGCG

Downstream 100 bases:

>100_bases
GGAACTCAGGTTCAAAACGTGACTCCAGGACAGAGTATGAGCCCGAAATCGGCCCTTTCCGCCGCCACCCTCCTCGACGA
GCTGCAATCCACGCTGGCGC

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 381; Mature: 380

Protein sequence:

>381_residues
MGSAAVISGMPAGTVDGATRARTLAVRARRMKQRRQMFDLVAASYLTDALLLLIYSFTGTIPEIVVPTYIACGFGIIVGS
IALSESGFYDRFKDHYLVLPTVALNVALVMGFCIWVPQIAFLFLCSLFIIFNFASLRASTSQSLICWATMAAALVVLLVF
TDTPIAIPHDTLLERTVTMLALVLTLGRCMFIGMYSSAMRVSLYKRGLQLREAYRRIEELAELDELTGAFNRRCIMRMLD
DEIARAERSATPLSLALIDLDWFKRINDSFGHPTGDEVLRTFAITIFANIRSFDRFGRYGGEEFLLLLPNTSREDAHALL
DRLREIVGELDWTAFSPGMRVTLSAGVTTMSPDETAESVLARADSALYAAKEKGRNRIATT

Sequences:

>Translated_381_residues
MGSAAVISGMPAGTVDGATRARTLAVRARRMKQRRQMFDLVAASYLTDALLLLIYSFTGTIPEIVVPTYIACGFGIIVGS
IALSESGFYDRFKDHYLVLPTVALNVALVMGFCIWVPQIAFLFLCSLFIIFNFASLRASTSQSLICWATMAAALVVLLVF
TDTPIAIPHDTLLERTVTMLALVLTLGRCMFIGMYSSAMRVSLYKRGLQLREAYRRIEELAELDELTGAFNRRCIMRMLD
DEIARAERSATPLSLALIDLDWFKRINDSFGHPTGDEVLRTFAITIFANIRSFDRFGRYGGEEFLLLLPNTSREDAHALL
DRLREIVGELDWTAFSPGMRVTLSAGVTTMSPDETAESVLARADSALYAAKEKGRNRIATT
>Mature_380_residues
GSAAVISGMPAGTVDGATRARTLAVRARRMKQRRQMFDLVAASYLTDALLLLIYSFTGTIPEIVVPTYIACGFGIIVGSI
ALSESGFYDRFKDHYLVLPTVALNVALVMGFCIWVPQIAFLFLCSLFIIFNFASLRASTSQSLICWATMAAALVVLLVFT
DTPIAIPHDTLLERTVTMLALVLTLGRCMFIGMYSSAMRVSLYKRGLQLREAYRRIEELAELDELTGAFNRRCIMRMLDD
EIARAERSATPLSLALIDLDWFKRINDSFGHPTGDEVLRTFAITIFANIRSFDRFGRYGGEEFLLLLPNTSREDAHALLD
RLREIVGELDWTAFSPGMRVTLSAGVTTMSPDETAESVLARADSALYAAKEKGRNRIATT

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=157, Percent_Identity=39.4904458598726, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1787262, Length=170, Percent_Identity=38.8235294117647, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1786584, Length=175, Percent_Identity=34.2857142857143, Blast_Score=104, Evalue=9e-24,
Organism=Escherichia coli, GI87081881, Length=187, Percent_Identity=34.7593582887701, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1787816, Length=159, Percent_Identity=37.1069182389937, Blast_Score=101, Evalue=8e-23,
Organism=Escherichia coli, GI1788381, Length=169, Percent_Identity=34.9112426035503, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI87082007, Length=160, Percent_Identity=37.5, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1787541, Length=176, Percent_Identity=32.9545454545455, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1787802, Length=171, Percent_Identity=28.6549707602339, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI87081977, Length=172, Percent_Identity=34.8837209302326, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI87081974, Length=154, Percent_Identity=27.2727272727273, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788085, Length=165, Percent_Identity=33.3333333333333, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1787056, Length=163, Percent_Identity=34.3558282208589, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 42236; Mature: 42105

Theoretical pI: Translated: 7.37; Mature: 7.37

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
GSAAVISGMPAGTVDGATRARTLAVRARRMKQRRQMFDLVAASYLTDALLLLIYSFTGT
CCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
IPEIVVPTYIACGFGIIVGSIALSESGFYDRFKDHYLVLPTVALNVALVMGFCIWVPQIA
CHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHH
FLFLCSLFIIFNFASLRASTSQSLICWATMAAALVVLLVFTDTPIAIPHDTLLERTVTML
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
ALVLTLGRCMFIGMYSSAMRVSLYKRGLQLREAYRRIEELAELDELTGAFNRRCIMRMLD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DEIARAERSATPLSLALIDLDWFKRINDSFGHPTGDEVLRTFAITIFANIRSFDRFGRYG
HHHHHHHCCCCCCEEEEECHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
GEEFLLLLPNTSREDAHALLDRLREIVGELDWTAFSPGMRVTLSAGVTTMSPDETAESVL
CCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCEECCCCEEEEECCCEECCCHHHHHHHH
ARADSALYAAKEKGRNRIATT
HHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA