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The map label for this gene is dusA [H]

Identifier: 86750510

GI number: 86750510

Start: 3885700

End: 3886698

Strand: Reverse

Name: dusA [H]

Synonym: RPB_3399

Alternate gene names: 86750510

Gene position: 3886698-3885700 (Counterclockwise)

Preceding gene: 86750511

Following gene: 86750504

Centisome position: 72.9

GC content: 67.97

Gene sequence:

>999_bases
ATGCAGACCACACAGATCCGCAGCTTCAGATTTTGTACCGCGCCGATGATGGAGTGGACCGATTCGCGCTGTCGCGTATT
CCATCGTCTGCTGACGAAGCGGGCGCGGCTGTACACCGAGATGGTGACCACCGGCGCGGTGATCCACGGCGATCGGCAGC
GCTTGCTCGGCTTCGATGCGTCCGAACATCCGGTGGCGCTGCAGCTCGGCGGCTCCGATCCGGCCGCGCTCGCGACCGCC
GCGAAGATCGGCGAGGATTTCGGCTACGACGAGATCAACCTCAATGTCGGTTGCCCGTCGGACCGGGTGAAGGAGGGCCG
GTTCGGCGCCTGCCTGATGGCGGAGCCCGAGCTGGTCGCCGACGGCGTCGCGGCGATGAAGCGCGCGGTGGAGATCCCGG
TGACGGTGAAATGCCGGATCGGCATCGACGATCAGGATCCGGAGGCGGCGCTCGATACGCTGGCGCGCGGTGTGGTCGCG
GCCGGCGTCGATGCGCTGATCGTGCATGCCCGCAAGGCCTGGCTCAACGGCCTGTCGCCGAAGGAAAACCGCCACGTCCC
GCCGCTCGACTACGATCGCGTGTACCGGCTGAAACGGGCGCTGCCCGATCTGCCGATCGTCATCAATGGCGGCATCGGCA
GTATCGCCGAGGCGAAGCTGCATCTGGCGCATGTCGATGGCGTGATGCTCGGCCGCGCCGCGTATCAGGAGCCGTGGCGG
CTGCTCGAGGTCGATCCGGAGCTGTTCGGCGAGGCCGCGCCTTACGCGACGATGAAAGACGCGCTCGAGGCGCTGATGCC
GTATATCGAGGACCAGCTCGCACAGGGCGTGCGGCTGCATGCGATCACCCGGCACCTGATCGGCGCGTATCAGGGCGTGC
CCGGCGCGCGCGCGTTCCGGCGCTATCTGTCGGAATTCGGCGTCAAGCCCGACGCCGACGCCAGCGTGTTGCGCAAGGCG
ATGGTGGCGGTCGAGCAGCGCGCCGCCGAGGCGGCGTGA

Upstream 100 bases:

>100_bases
ACCTCTCGATCGACCCGGCGCTCGCGGCGACGCAGCCATACCGACCCAGCGTCGAACCGACTGGTGCACGACGTCTTCGA
ATAATTGATTGAATCGAACG

Downstream 100 bases:

>100_bases
ACGGCAGTCGTTCGGCTATCAGGACACGCGCAGCGCGCTTTCTTCACCCTCCCCTCGAGGGGGAGGGTCGGCACGCAGCC
CGCAGAGCGGGATGCGTGAC

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MQTTQIRSFRFCTAPMMEWTDSRCRVFHRLLTKRARLYTEMVTTGAVIHGDRQRLLGFDASEHPVALQLGGSDPAALATA
AKIGEDFGYDEINLNVGCPSDRVKEGRFGACLMAEPELVADGVAAMKRAVEIPVTVKCRIGIDDQDPEAALDTLARGVVA
AGVDALIVHARKAWLNGLSPKENRHVPPLDYDRVYRLKRALPDLPIVINGGIGSIAEAKLHLAHVDGVMLGRAAYQEPWR
LLEVDPELFGEAAPYATMKDALEALMPYIEDQLAQGVRLHAITRHLIGAYQGVPGARAFRRYLSEFGVKPDADASVLRKA
MVAVEQRAAEAA

Sequences:

>Translated_332_residues
MQTTQIRSFRFCTAPMMEWTDSRCRVFHRLLTKRARLYTEMVTTGAVIHGDRQRLLGFDASEHPVALQLGGSDPAALATA
AKIGEDFGYDEINLNVGCPSDRVKEGRFGACLMAEPELVADGVAAMKRAVEIPVTVKCRIGIDDQDPEAALDTLARGVVA
AGVDALIVHARKAWLNGLSPKENRHVPPLDYDRVYRLKRALPDLPIVINGGIGSIAEAKLHLAHVDGVMLGRAAYQEPWR
LLEVDPELFGEAAPYATMKDALEALMPYIEDQLAQGVRLHAITRHLIGAYQGVPGARAFRRYLSEFGVKPDADASVLRKA
MVAVEQRAAEAA
>Mature_332_residues
MQTTQIRSFRFCTAPMMEWTDSRCRVFHRLLTKRARLYTEMVTTGAVIHGDRQRLLGFDASEHPVALQLGGSDPAALATA
AKIGEDFGYDEINLNVGCPSDRVKEGRFGACLMAEPELVADGVAAMKRAVEIPVTVKCRIGIDDQDPEAALDTLARGVVA
AGVDALIVHARKAWLNGLSPKENRHVPPLDYDRVYRLKRALPDLPIVINGGIGSIAEAKLHLAHVDGVMLGRAAYQEPWR
LLEVDPELFGEAAPYATMKDALEALMPYIEDQLAQGVRLHAITRHLIGAYQGVPGARAFRRYLSEFGVKPDADASVLRKA
MVAVEQRAAEAA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusA subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=270, Percent_Identity=29.2592592592593, Blast_Score=86, Evalue=3e-17,
Organism=Escherichia coli, GI145693211, Length=321, Percent_Identity=58.5669781931464, Blast_Score=367, Evalue=1e-103,
Organism=Escherichia coli, GI1789660, Length=327, Percent_Identity=27.82874617737, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1788462, Length=188, Percent_Identity=31.3829787234043, Blast_Score=79, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI25144369, Length=264, Percent_Identity=29.1666666666667, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323560, Length=232, Percent_Identity=26.2931034482759, Blast_Score=77, Evalue=3e-15,
Organism=Drosophila melanogaster, GI19921524, Length=257, Percent_Identity=24.9027237354086, Blast_Score=73, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: 1.-.-.-

Molecular weight: Translated: 36288; Mature: 36288

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTTQIRSFRFCTAPMMEWTDSRCRVFHRLLTKRARLYTEMVTTGAVIHGDRQRLLGFDA
CCCCCCCCEEECCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCEEECCHHHHHCCCC
SEHPVALQLGGSDPAALATAAKIGEDFGYDEINLNVGCPSDRVKEGRFGACLMAEPELVA
CCCCEEEEECCCCHHHHHHHHHHHHCCCCCEEEEEECCCHHHHCCCCCCEEEECCCHHHH
DGVAAMKRAVEIPVTVKCRIGIDDQDPEAALDTLARGVVAAGVDALIVHARKAWLNGLSP
HHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
KENRHVPPLDYDRVYRLKRALPDLPIVINGGIGSIAEAKLHLAHVDGVMLGRAAYQEPWR
CCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHCHHHHHHHHHHCCCE
LLEVDPELFGEAAPYATMKDALEALMPYIEDQLAQGVRLHAITRHLIGAYQGVPGARAFR
EEEECHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHH
RYLSEFGVKPDADASVLRKAMVAVEQRAAEAA
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQTTQIRSFRFCTAPMMEWTDSRCRVFHRLLTKRARLYTEMVTTGAVIHGDRQRLLGFDA
CCCCCCCCEEECCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCEEECCHHHHHCCCC
SEHPVALQLGGSDPAALATAAKIGEDFGYDEINLNVGCPSDRVKEGRFGACLMAEPELVA
CCCCEEEEECCCCHHHHHHHHHHHHCCCCCEEEEEECCCHHHHCCCCCCEEEECCCHHHH
DGVAAMKRAVEIPVTVKCRIGIDDQDPEAALDTLARGVVAAGVDALIVHARKAWLNGLSP
HHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
KENRHVPPLDYDRVYRLKRALPDLPIVINGGIGSIAEAKLHLAHVDGVMLGRAAYQEPWR
CCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHCHHHHHHHHHHCCCE
LLEVDPELFGEAAPYATMKDALEALMPYIEDQLAQGVRLHAITRHLIGAYQGVPGARAFR
EEEECHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHH
RYLSEFGVKPDADASVLRKAMVAVEQRAAEAA
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]