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The map label for this gene is cysS

Identifier: 86750485

GI number: 86750485

Start: 3857649

End: 3859130

Strand: Reverse

Name: cysS

Synonym: RPB_3374

Alternate gene names: 86750485

Gene position: 3859130-3857649 (Counterclockwise)

Preceding gene: 86750489

Following gene: 86750484

Centisome position: 72.38

GC content: 61.88

Gene sequence:

>1482_bases
ATGGCTTTGCGCCTCTACGACACGCTGACCAAAGAGAAGCGCGCCTTCGCGCCGATCGATCCGTCGAACGTGCGGATGTA
TGTCTGCGGGCCGACGGTCTACGACTTCGCCCATATCGGCAATGCCCGGCCGGTCATCGTGTTCGACGTGCTGTTCCGGC
TGCTGCGGCATCTCTACGGCGAAAACCACGTCACCTACGTCCGCAACATCACCGACGTCGACGACAAGATCAACGACCGC
GCCGCGCGTGACTATCCCGGCCTGCCGCTGAACGAGGCGATCCGCAAGGTCACCGAGCAGACCGAGCGGCAATTCCACGA
CGACGTCGATGCGTTGGGCTGCCTGCGGCCGACCGTCGAGCCGCGCGCGACCGAACACATCGGCGAGATGCGCACCATCA
TCGACCGGCTGGTCGCCGGCGGCTTCGCCTATGTCGCCGCGGACCACGTGCTGTTCTCGCCGGCGGCGATGAACGCTGCG
AACAGCGTGCTGCCGCGCTACGGCGCGCTGGCCAACCGCTCGCTCGACGAGATGATCGCCGGCGCCCGCGTCGACGTCGC
CCCCTACAAGCGCGACGCCACCGACTTCGTGCTGTGGAAGCCGTCGAAGCCCGGCGAGCCGTCCTGGCCGTCGCCGGCCG
GCATCACGATGGAAGGACGTCCCGGCTGGCACATCGAATGCTCGGCGATGTCGTGGAAGCATCTCGGCGAGACCTTCGAC
ATCCACGGCGGCGGCATCGACCTGGTGTTTCCGCATCACGAAAACGAAGTCGCGCAAAGCTGCTGCGCCTTCCAGACCGA
CCGCATGGCCCAGACCTGGATGCACAACGGCTTCCTACAGGTCGAAGGCGAGAAGATGTCGAAGAGCCTGGGCAACTTCA
TCACGATCAGGGAGTTGCTGGCGACGGAGAAATTCGGGGGAGATAGTTGGGTTGGTGAGATTCTTCGATTTGCGATGATT
AAAACTCACTACCGCTCACCGATCGACTGGACCGTGAAGGCGCTCGACGAGGGTCATAAGGTTCTTTGGGATTGGTATCG
CGACATTGGTGACGTCGGGCCGGCACAGCAACTGCCGGGAGAATTCATCGACTGTTTGGCTGATGATCTCAACATATCGA
GTGCCATCGCATTCATGCACAGCCTGCGTAAAGATAAGAAGTTTGCTGAGCTTCTTGCGACGATGAACTTTCTTGGATTC
TCGAATGCGGAATCGGTTTTGGCGCGTCGCCCTGTTGGAGTTCGGATTAATCTTCCCCCTGCGCACGCCGAGGCGGCCGT
CGGAACAGTGGAAGTACTCGCAAAGCCCTTGAGCAAGAGCGAGATTGAAGAACGGATCGACGCCCGAACCGCCGCCCGCG
CGCGAAAAGATTTCAAGGAATCCGATCGCATCCGCGACGAGCTCGCCGCGATGGGCATCGCGATCAAGGACGGCAAGGAC
GCCGACGGCAAGCCGGTGACGACCTGGGAGATCGCGCGATGA

Upstream 100 bases:

>100_bases
GTGGCATCTGCCGCGAGATTACGAGCTAGCTCAACGCTGGCTGAGGCCGTCTTTTCTCTCATGAGATCACTGGACGCCCG
GCCGCAGGGGACGGGAATCC

Downstream 100 bases:

>100_bases
GCGGCGCCACGCCGCTGCGTCATCCTGAGGTGCGCGCGCGGTTGCGCGCGCCTCGAAGGATGGGCTGCAGGGACAATGCC
CGATCCGCCGCGCCCGGAAT

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 493; Mature: 492

Protein sequence:

>493_residues
MALRLYDTLTKEKRAFAPIDPSNVRMYVCGPTVYDFAHIGNARPVIVFDVLFRLLRHLYGENHVTYVRNITDVDDKINDR
AARDYPGLPLNEAIRKVTEQTERQFHDDVDALGCLRPTVEPRATEHIGEMRTIIDRLVAGGFAYVAADHVLFSPAAMNAA
NSVLPRYGALANRSLDEMIAGARVDVAPYKRDATDFVLWKPSKPGEPSWPSPAGITMEGRPGWHIECSAMSWKHLGETFD
IHGGGIDLVFPHHENEVAQSCCAFQTDRMAQTWMHNGFLQVEGEKMSKSLGNFITIRELLATEKFGGDSWVGEILRFAMI
KTHYRSPIDWTVKALDEGHKVLWDWYRDIGDVGPAQQLPGEFIDCLADDLNISSAIAFMHSLRKDKKFAELLATMNFLGF
SNAESVLARRPVGVRINLPPAHAEAAVGTVEVLAKPLSKSEIEERIDARTAARARKDFKESDRIRDELAAMGIAIKDGKD
ADGKPVTTWEIAR

Sequences:

>Translated_493_residues
MALRLYDTLTKEKRAFAPIDPSNVRMYVCGPTVYDFAHIGNARPVIVFDVLFRLLRHLYGENHVTYVRNITDVDDKINDR
AARDYPGLPLNEAIRKVTEQTERQFHDDVDALGCLRPTVEPRATEHIGEMRTIIDRLVAGGFAYVAADHVLFSPAAMNAA
NSVLPRYGALANRSLDEMIAGARVDVAPYKRDATDFVLWKPSKPGEPSWPSPAGITMEGRPGWHIECSAMSWKHLGETFD
IHGGGIDLVFPHHENEVAQSCCAFQTDRMAQTWMHNGFLQVEGEKMSKSLGNFITIRELLATEKFGGDSWVGEILRFAMI
KTHYRSPIDWTVKALDEGHKVLWDWYRDIGDVGPAQQLPGEFIDCLADDLNISSAIAFMHSLRKDKKFAELLATMNFLGF
SNAESVLARRPVGVRINLPPAHAEAAVGTVEVLAKPLSKSEIEERIDARTAARARKDFKESDRIRDELAAMGIAIKDGKD
ADGKPVTTWEIAR
>Mature_492_residues
ALRLYDTLTKEKRAFAPIDPSNVRMYVCGPTVYDFAHIGNARPVIVFDVLFRLLRHLYGENHVTYVRNITDVDDKINDRA
ARDYPGLPLNEAIRKVTEQTERQFHDDVDALGCLRPTVEPRATEHIGEMRTIIDRLVAGGFAYVAADHVLFSPAAMNAAN
SVLPRYGALANRSLDEMIAGARVDVAPYKRDATDFVLWKPSKPGEPSWPSPAGITMEGRPGWHIECSAMSWKHLGETFDI
HGGGIDLVFPHHENEVAQSCCAFQTDRMAQTWMHNGFLQVEGEKMSKSLGNFITIRELLATEKFGGDSWVGEILRFAMIK
THYRSPIDWTVKALDEGHKVLWDWYRDIGDVGPAQQLPGEFIDCLADDLNISSAIAFMHSLRKDKKFAELLATMNFLGFS
NAESVLARRPVGVRINLPPAHAEAAVGTVEVLAKPLSKSEIEERIDARTAARARKDFKESDRIRDELAAMGIAIKDGKDA
DGKPVTTWEIAR

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=337, Percent_Identity=39.1691394658754, Blast_Score=208, Evalue=9e-54,
Organism=Homo sapiens, GI10835051, Length=273, Percent_Identity=37.3626373626374, Blast_Score=180, Evalue=3e-45,
Organism=Homo sapiens, GI62240992, Length=273, Percent_Identity=37.3626373626374, Blast_Score=179, Evalue=4e-45,
Organism=Homo sapiens, GI21269877, Length=273, Percent_Identity=37.3626373626374, Blast_Score=179, Evalue=4e-45,
Organism=Homo sapiens, GI303304987, Length=273, Percent_Identity=37.3626373626374, Blast_Score=179, Evalue=5e-45,
Organism=Escherichia coli, GI1786737, Length=496, Percent_Identity=40.9274193548387, Blast_Score=320, Evalue=9e-89,
Organism=Caenorhabditis elegans, GI17509797, Length=247, Percent_Identity=36.4372469635627, Blast_Score=147, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17509795, Length=211, Percent_Identity=41.2322274881517, Blast_Score=147, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6324082, Length=432, Percent_Identity=30.3240740740741, Blast_Score=168, Evalue=2e-42,
Organism=Drosophila melanogaster, GI19922218, Length=400, Percent_Identity=35.25, Blast_Score=213, Evalue=2e-55,
Organism=Drosophila melanogaster, GI19922364, Length=260, Percent_Identity=37.6923076923077, Blast_Score=172, Evalue=4e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_RHOP2 (Q2IUP0)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_486981.1
- ProteinModelPortal:   Q2IUP0
- SMR:   Q2IUP0
- STRING:   Q2IUP0
- GeneID:   3911176
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_3374
- eggNOG:   COG0215
- HOGENOM:   HBG327651
- OMA:   SERIFYI
- ProtClustDB:   PRK00260
- BioCyc:   RPAL316058:RPB_3374-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00041_B
- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- SMART:   SM00840
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 55082; Mature: 54951

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 288-288

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALRLYDTLTKEKRAFAPIDPSNVRMYVCGPTVYDFAHIGNARPVIVFDVLFRLLRHLYG
CEEEHHHHHHHHHCCCCCCCCCCEEEEEECCHHHHHHHCCCCCCCHHHHHHHHHHHHHHC
ENHVTYVRNITDVDDKINDRAARDYPGLPLNEAIRKVTEQTERQFHDDVDALGCLRPTVE
CCCEEEEECCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PRATEHIGEMRTIIDRLVAGGFAYVAADHVLFSPAAMNAANSVLPRYGALANRSLDEMIA
CHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHC
GARVDVAPYKRDATDFVLWKPSKPGEPSWPSPAGITMEGRPGWHIECSAMSWKHLGETFD
CCCEECCCCCCCCCCEEEECCCCCCCCCCCCCCCEEECCCCCCEEEECCCCHHHCCCEEE
IHGGGIDLVFPHHENEVAQSCCAFQTDRMAQTWMHNGFLQVEGEKMSKSLGNFITIRELL
ECCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHHH
ATEKFGGDSWVGEILRFAMIKTHYRSPIDWTVKALDEGHKVLWDWYRDIGDVGPAQQLPG
HHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHCCCCCHHHCCH
EFIDCLADDLNISSAIAFMHSLRKDKKFAELLATMNFLGFSNAESVLARRPVGVRINLPP
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCEEEECCC
AHAEAAVGTVEVLAKPLSKSEIEERIDARTAARARKDFKESDRIRDELAAMGIAIKDGKD
CHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCC
ADGKPVTTWEIAR
CCCCCCCEEECCC
>Mature Secondary Structure 
ALRLYDTLTKEKRAFAPIDPSNVRMYVCGPTVYDFAHIGNARPVIVFDVLFRLLRHLYG
EEEHHHHHHHHHCCCCCCCCCCEEEEEECCHHHHHHHCCCCCCCHHHHHHHHHHHHHHC
ENHVTYVRNITDVDDKINDRAARDYPGLPLNEAIRKVTEQTERQFHDDVDALGCLRPTVE
CCCEEEEECCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PRATEHIGEMRTIIDRLVAGGFAYVAADHVLFSPAAMNAANSVLPRYGALANRSLDEMIA
CHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHC
GARVDVAPYKRDATDFVLWKPSKPGEPSWPSPAGITMEGRPGWHIECSAMSWKHLGETFD
CCCEECCCCCCCCCCEEEECCCCCCCCCCCCCCCEEECCCCCCEEEECCCCHHHCCCEEE
IHGGGIDLVFPHHENEVAQSCCAFQTDRMAQTWMHNGFLQVEGEKMSKSLGNFITIRELL
ECCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHHH
ATEKFGGDSWVGEILRFAMIKTHYRSPIDWTVKALDEGHKVLWDWYRDIGDVGPAQQLPG
HHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHCCCCCHHHCCH
EFIDCLADDLNISSAIAFMHSLRKDKKFAELLATMNFLGFSNAESVLARRPVGVRINLPP
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCEEEECCC
AHAEAAVGTVEVLAKPLSKSEIEERIDARTAARARKDFKESDRIRDELAAMGIAIKDGKD
CHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCC
ADGKPVTTWEIAR
CCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA