Click here to switch to the map view.

The map label for this gene is ilvI [H]

Identifier: 86750456

GI number: 86750456

Start: 3825959

End: 3827737

Strand: Reverse

Name: ilvI [H]

Synonym: RPB_3345

Alternate gene names: 86750456

Gene position: 3827737-3825959 (Counterclockwise)

Preceding gene: 86750457

Following gene: 86750455

Centisome position: 71.79

GC content: 65.77

Gene sequence:

>1779_bases
ATGAGCGACAGCACCAGCCACGATCCCAACCAGATGACCGGCGCCGCGATGATCGTCCGCGCGATGAAGGATCACGGCGT
CGAGCACATCTTCGGCTATCCCGGCGGCGCGGTGCTTCCGATCTATGACGAGATCTTCCAGCAGTCCGACGTGCAGCACA
TCCTGGTCCGGCACGAGCAGGGCGCCGGCCATGCCGCCGAGGGCTATGCGCGCTCGACCGGCAAGCCGGGCGTGGTGCTG
GTGACCTCGGGCCCGGGCGCCACCAACATGGTGACGCCGCTGGCCGACGCGCTGATGGATTCGATCCCGATCGTCTGCAT
CACCGGGCAGGTGCCGACGCATCTGATCGGCAATGACGCGTTCCAGGAATGCGACACCGTCGGCATCACCCGGCCTTGCA
CCAAGCACAATTGGTTGGTGCGCGACATCAAGGATCTCGCCCGCGTGCTGCACGAGGCGTTCTACGTCGCGACCTCCGGC
CGTCCGGGCCCGGTCGTGGTCGACGTGCCGAAGGACGTGCAGTTCGCCACCGGCACCTATCATCCGCCGCGCAAGGACGA
CGTCCACGTCTCCTACAAGCCGGTCACCAAGGGCGATCCGAACCAGATCCGCAAGGCGGTGGCGATGCTCGCCGGCGCCA
AGCGGCCGGTGATCTATTCCGGCGGCGGCGTGGTCAATTCCGGCGACGAAGCCTGCCGGCTGCTGCGCGAGCTGGTCGAG
GTCACCGATTTCCCGATCACCTCGACGCTGATGGGGCTCGGCGCCTATCCGGCGTCGGGCAAGAACTGGCTCGGCATGCT
CGGCATGCACGGCACCTACGAAGCCAACATGACGATGCATGGCTGCGACGTCATGCTGTGCATCGGCGCGCGCTTCGACG
ACCGCATCACCGGCCGCACCGACGCGTTCGCGCCGCACGCCAAGAAGATCCACATCGACATCGACCCGTCGTCGATCAAC
AAGAACATCCGCGTCGACGTGCCGATCATCGGCGACGTCGCCAGCGTGCTGACCGATCTGCTCGCGGTGTTCAAGGCCGA
GGCGAAGAAGCCCGACATCAAGCCGTGGTGGCACCAGGTCGCGACCTGGCGCGCGCGCAATTCGCTGGCCTACAAGAAGA
ACAACGACCTGATCATGCCGCAATACGCGATCCAGCGGCTTTACGAGGCGACGCGTGGCCGCGACACCTACATCACCACC
GAAGTCGGCCAGCATCAGATGTGGGCGGCGCAGTTCTACGGCTTCGAACAGCCGAAGCGCTGGATGACCTCGGGCGGCCT
CGGCACCATGGGCTACGGCCTGCCGGCGGCGCTCGGCGTCCAGGTGGCGCATCGCGATGCGCTGGTGATCGACATCGCCG
GCGACGCCTCGGTGCAGATGACGATGCAGGAGATGGCGACGGCGGTGCAGTACGAACTGCCGATCAAGATCTTCATCCTC
AACAACCAGTATATGGGGATGGTGCGGCAGTGGCAGCAGCTGTTGCATGGTAATCGGCTGTCGCACTCCTACACCGAGGC
GATGCCGGACTTCGTCAAGCTCGCCGAGGCCTATGGCGGCGTCGGCATGCAGGTGACCAAGCCGGCCGATCTCGACGGCG
CGATCATGGACATGATCAAGGTCAACAAGCCGGTGCTGTTCGACTGCCGCGTCGCCGCGCTGGAGAACTGCTTCCCGATG
ATCCCGTCCGGCAAGGCCCACAACGAGATGCTGCTGCCGGCCGAAGCCACCGACGAAGCCACCGCGGCGGCCTTCGCCGG
CGGCAAGGCGCTGGTGTGA

Upstream 100 bases:

>100_bases
GGCCTTGAACGGGCCCTTTTTTATTGTCCGAACGCCTTCGACCCGAACTTTGTCCGAACCACCCGAACTTCAGCGCCAGA
CGGCGCCTCCGGAGCCCACC

Downstream 100 bases:

>100_bases
GCCACTGACGCGCTGCGCTGATTACCTCTCCCCGCGTGCGGGGAGAGGTCGACCCGGCGGAGCGTAGCGAAGCCGGGGCG
GGTGAGGGGGCGCCTCCGCG

Product: acetolactate synthase 3 catalytic subunit

Products: NA

Alternate protein names: AHAS-III; ALS-III; Acetohydroxy-acid synthase III large subunit [H]

Number of amino acids: Translated: 592; Mature: 591

Protein sequence:

>592_residues
MSDSTSHDPNQMTGAAMIVRAMKDHGVEHIFGYPGGAVLPIYDEIFQQSDVQHILVRHEQGAGHAAEGYARSTGKPGVVL
VTSGPGATNMVTPLADALMDSIPIVCITGQVPTHLIGNDAFQECDTVGITRPCTKHNWLVRDIKDLARVLHEAFYVATSG
RPGPVVVDVPKDVQFATGTYHPPRKDDVHVSYKPVTKGDPNQIRKAVAMLAGAKRPVIYSGGGVVNSGDEACRLLRELVE
VTDFPITSTLMGLGAYPASGKNWLGMLGMHGTYEANMTMHGCDVMLCIGARFDDRITGRTDAFAPHAKKIHIDIDPSSIN
KNIRVDVPIIGDVASVLTDLLAVFKAEAKKPDIKPWWHQVATWRARNSLAYKKNNDLIMPQYAIQRLYEATRGRDTYITT
EVGQHQMWAAQFYGFEQPKRWMTSGGLGTMGYGLPAALGVQVAHRDALVIDIAGDASVQMTMQEMATAVQYELPIKIFIL
NNQYMGMVRQWQQLLHGNRLSHSYTEAMPDFVKLAEAYGGVGMQVTKPADLDGAIMDMIKVNKPVLFDCRVAALENCFPM
IPSGKAHNEMLLPAEATDEATAAAFAGGKALV

Sequences:

>Translated_592_residues
MSDSTSHDPNQMTGAAMIVRAMKDHGVEHIFGYPGGAVLPIYDEIFQQSDVQHILVRHEQGAGHAAEGYARSTGKPGVVL
VTSGPGATNMVTPLADALMDSIPIVCITGQVPTHLIGNDAFQECDTVGITRPCTKHNWLVRDIKDLARVLHEAFYVATSG
RPGPVVVDVPKDVQFATGTYHPPRKDDVHVSYKPVTKGDPNQIRKAVAMLAGAKRPVIYSGGGVVNSGDEACRLLRELVE
VTDFPITSTLMGLGAYPASGKNWLGMLGMHGTYEANMTMHGCDVMLCIGARFDDRITGRTDAFAPHAKKIHIDIDPSSIN
KNIRVDVPIIGDVASVLTDLLAVFKAEAKKPDIKPWWHQVATWRARNSLAYKKNNDLIMPQYAIQRLYEATRGRDTYITT
EVGQHQMWAAQFYGFEQPKRWMTSGGLGTMGYGLPAALGVQVAHRDALVIDIAGDASVQMTMQEMATAVQYELPIKIFIL
NNQYMGMVRQWQQLLHGNRLSHSYTEAMPDFVKLAEAYGGVGMQVTKPADLDGAIMDMIKVNKPVLFDCRVAALENCFPM
IPSGKAHNEMLLPAEATDEATAAAFAGGKALV
>Mature_591_residues
SDSTSHDPNQMTGAAMIVRAMKDHGVEHIFGYPGGAVLPIYDEIFQQSDVQHILVRHEQGAGHAAEGYARSTGKPGVVLV
TSGPGATNMVTPLADALMDSIPIVCITGQVPTHLIGNDAFQECDTVGITRPCTKHNWLVRDIKDLARVLHEAFYVATSGR
PGPVVVDVPKDVQFATGTYHPPRKDDVHVSYKPVTKGDPNQIRKAVAMLAGAKRPVIYSGGGVVNSGDEACRLLRELVEV
TDFPITSTLMGLGAYPASGKNWLGMLGMHGTYEANMTMHGCDVMLCIGARFDDRITGRTDAFAPHAKKIHIDIDPSSINK
NIRVDVPIIGDVASVLTDLLAVFKAEAKKPDIKPWWHQVATWRARNSLAYKKNNDLIMPQYAIQRLYEATRGRDTYITTE
VGQHQMWAAQFYGFEQPKRWMTSGGLGTMGYGLPAALGVQVAHRDALVIDIAGDASVQMTMQEMATAVQYELPIKIFILN
NQYMGMVRQWQQLLHGNRLSHSYTEAMPDFVKLAEAYGGVGMQVTKPADLDGAIMDMIKVNKPVLFDCRVAALENCFPMI
PSGKAHNEMLLPAEATDEATAAAFAGGKALV

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=568, Percent_Identity=25.7042253521127, Blast_Score=166, Evalue=5e-41,
Organism=Homo sapiens, GI21361361, Length=552, Percent_Identity=24.6376811594203, Blast_Score=144, Evalue=2e-34,
Organism=Escherichia coli, GI87081685, Length=568, Percent_Identity=50, Blast_Score=593, Evalue=1e-171,
Organism=Escherichia coli, GI1790104, Length=571, Percent_Identity=42.5569176882662, Blast_Score=461, Evalue=1e-131,
Organism=Escherichia coli, GI1786717, Length=534, Percent_Identity=37.2659176029963, Blast_Score=316, Evalue=2e-87,
Organism=Escherichia coli, GI1787096, Length=556, Percent_Identity=27.3381294964029, Blast_Score=192, Evalue=7e-50,
Organism=Escherichia coli, GI1788716, Length=561, Percent_Identity=28.1639928698752, Blast_Score=169, Evalue=5e-43,
Organism=Caenorhabditis elegans, GI17531301, Length=571, Percent_Identity=25.569176882662, Blast_Score=159, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI17531299, Length=571, Percent_Identity=25.569176882662, Blast_Score=159, Evalue=5e-39,
Organism=Caenorhabditis elegans, GI17542570, Length=520, Percent_Identity=26.1538461538462, Blast_Score=122, Evalue=7e-28,
Organism=Saccharomyces cerevisiae, GI6323755, Length=580, Percent_Identity=44.1379310344828, Blast_Score=486, Evalue=1e-138,
Organism=Saccharomyces cerevisiae, GI6320816, Length=491, Percent_Identity=24.847250509165, Blast_Score=106, Evalue=1e-23,
Organism=Drosophila melanogaster, GI19922626, Length=481, Percent_Identity=26.8191268191268, Blast_Score=165, Evalue=7e-41,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 64495; Mature: 64364

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDSTSHDPNQMTGAAMIVRAMKDHGVEHIFGYPGGAVLPIYDEIFQQSDVQHILVRHEQ
CCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCEEHHHHHHHCCCCHHEEEEEECC
GAGHAAEGYARSTGKPGVVLVTSGPGATNMVTPLADALMDSIPIVCITGQVPTHLIGNDA
CCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCEEEEECCCCCHHCCCHH
FQECDTVGITRPCTKHNWLVRDIKDLARVLHEAFYVATSGRPGPVVVDVPKDVQFATGTY
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEECCCCCCEEEECCCCCEECCCCC
HPPRKDDVHVSYKPVTKGDPNQIRKAVAMLAGAKRPVIYSGGGVVNSGDEACRLLRELVE
CCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHH
VTDFPITSTLMGLGAYPASGKNWLGMLGMHGTYEANMTMHGCDVMLCIGARFDDRITGRT
HCCCCHHHHHHHCCCCCCCCCCCEEEECCCCEEECCEEEECCCEEEEECCCCCCCCCCCC
DAFAPHAKKIHIDIDPSSINKNIRVDVPIIGDVASVLTDLLAVFKAEAKKPDIKPWWHQV
CCCCCCCEEEEEEECHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
ATWRARNSLAYKKNNDLIMPQYAIQRLYEATRGRDTYITTEVGQHQMWAAQFYGFEQPKR
HHHHHHCCEEEECCCCEECHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHCCCCCCHH
WMTSGGLGTMGYGLPAALGVQVAHRDALVIDIAGDASVQMTMQEMATAVQYELPIKIFIL
HHHCCCCCCCCCCCHHHHHEEEECCCEEEEEECCCCCHHHHHHHHHHHHEEECCEEEEEE
NNQYMGMVRQWQQLLHGNRLSHSYTEAMPDFVKLAEAYGGVGMQVTKPADLDGAIMDMIK
CCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHH
VNKPVLFDCRVAALENCFPMIPSGKAHNEMLLPAEATDEATAAAFAGGKALV
CCCCEEEEHHHHHHHHCCCCCCCCCCCCCEECCCCCCCCHHHHHHCCCCCCC
>Mature Secondary Structure 
SDSTSHDPNQMTGAAMIVRAMKDHGVEHIFGYPGGAVLPIYDEIFQQSDVQHILVRHEQ
CCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCEEHHHHHHHCCCCHHEEEEEECC
GAGHAAEGYARSTGKPGVVLVTSGPGATNMVTPLADALMDSIPIVCITGQVPTHLIGNDA
CCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCEEEEECCCCCHHCCCHH
FQECDTVGITRPCTKHNWLVRDIKDLARVLHEAFYVATSGRPGPVVVDVPKDVQFATGTY
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEECCCCCCEEEECCCCCEECCCCC
HPPRKDDVHVSYKPVTKGDPNQIRKAVAMLAGAKRPVIYSGGGVVNSGDEACRLLRELVE
CCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHH
VTDFPITSTLMGLGAYPASGKNWLGMLGMHGTYEANMTMHGCDVMLCIGARFDDRITGRT
HCCCCHHHHHHHCCCCCCCCCCCEEEECCCCEEECCEEEECCCEEEEECCCCCCCCCCCC
DAFAPHAKKIHIDIDPSSINKNIRVDVPIIGDVASVLTDLLAVFKAEAKKPDIKPWWHQV
CCCCCCCEEEEEEECHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
ATWRARNSLAYKKNNDLIMPQYAIQRLYEATRGRDTYITTEVGQHQMWAAQFYGFEQPKR
HHHHHHCCEEEECCCCEECHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHCCCCCCHH
WMTSGGLGTMGYGLPAALGVQVAHRDALVIDIAGDASVQMTMQEMATAVQYELPIKIFIL
HHHCCCCCCCCCCCHHHHHEEEECCCEEEEEECCCCCHHHHHHHHHHHHEEECCEEEEEE
NNQYMGMVRQWQQLLHGNRLSHSYTEAMPDFVKLAEAYGGVGMQVTKPADLDGAIMDMIK
CCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHH
VNKPVLFDCRVAALENCFPMIPSGKAHNEMLLPAEATDEATAAAFAGGKALV
CCCCEEEEHHHHHHHHCCCCCCCCCCCCCEECCCCCCCCHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6308579; 1630901; 9278503; 3891724; 9298646 [H]