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The map label for this gene is ageIM [H]

Identifier: 86750436

GI number: 86750436

Start: 3803548

End: 3804864

Strand: Reverse

Name: ageIM [H]

Synonym: RPB_3325

Alternate gene names: 86750436

Gene position: 3804864-3803548 (Counterclockwise)

Preceding gene: 86750437

Following gene: 86750435

Centisome position: 71.36

GC content: 56.42

Gene sequence:

>1317_bases
GTGCCTCAAGTCCCGTTCAGTGTTGTGGATTTATTCTGTGGGGCTGGGGGCCTTTCTCAAGGCTTCCGAGATGCCGGCTT
TAGGGTTGTGGCAGGCTCTGACAATGATCCGGACGCCATGGCGACCTACAGGGCAAATTTCCCTGAGGCTGCCGGCATCA
CTGGGGACATCCGGTCTGCCCCTATCAAGGAGCAACTTCTAGAGGCAGCGAGGCGCGCCACGGTGTTGATCGGGGGGCCG
CCCTGCCAAGCATTCTCTCAGGTTCGCAATCACACCCGGATGATTGACGATCCTCGGAACTCGCTCTACCGAGAGTTTGT
CGACGTTCTCAAACAATCTTTGCCACCCGCTTTTCTTATTGAGAACGTTACGGGCATGGACCAGATGGGCGTTCGTGATC
AAATCGCGTCCGACCTGTCCCTTGATGGCGAATATACCGTACTTCCGCAAGTTGTGGACGCTGCCGATTTCGGCGTTCCG
CAGACTCGAAAGCGCTTGCTCTTCGTCGGAGTGCGATCTCGCAGCCGAATGTCACCGCCCATCCTTATGGGGGCGGGAGC
CACTGATGCCTTTGCACTTGAGCGAGTCAGCGCTGGAGGGCGCGCTCGTTATCGAGTGGTGTTTAAAGAAAGCGCTTGGG
GTGAGCGCGCAAGTGCGATTCTTGCTGATCCCGACAATCTATCGGTTGTGAGTGCCGCAGACGCTATTGGTGATCTTGCC
TGCCTACCTCTCGGCAACCGTCAAGATATCATCCCGTACAGCGATCTGCCTGACGCGCAAAGTGCTTACCAACGCCTCAT
GCGCAAAGGAGGCAAGGCTACGCTCTCTAATGTTCAAGTGCCTCGCATCAATGAAGATACTAAGCTGCGGCTGGGCGGCA
TCCCGCCGGGCGGAAATTATCGCGATCTGCGAGCAGGGTTGCAAGAGAGATATATAACGGGGCAACGGTGGGGGCAGGAT
AACGGCTCCGGAATGCTATCTAGAAAGCATTTTTATGCCTACCGTCGCCTTCATCCCGATTTGTGGGCCTGGACGCTCAA
TACAAAGGGCGATGCCGTGTATCACTACAATAAGCTTCGAGCTTTGTCGGTCCGCGAGTTCGCTCGGCTTCAGTCCTTTC
CAGATCGCTTCGTATTCACGACCGATCAGCGTCGCGGGAAAATTGAAGGACGGCACGACGGCGGTCCTGCTCACTCCAGA
TACCGCCAAGTTGGAAACGCCGTTCCGCCCCTGCTTGCGCGTGCAGTTGCAGAAAGTCTGATCGCGCAGCTCAAGTTGCT
TGATTTCACCGATCGAAGCCTCGTTGCGGCAGAGTAA

Upstream 100 bases:

>100_bases
CTGTAGAGGGCGTATGTCGAGGGGAGTCGTGTTGGACATGCGCTGCGCAGAACCGAAAAAGCGCGAAGCCCCCGCTGCCG
CACGAGCGCTGGCTAAGAGT

Downstream 100 bases:

>100_bases
TGTCTGACCAAGCTGCTCAAGAGAAGGTCTCAGCCAAGACCAGCTCAATCAACTCAGCATTCGGTGCCAAGAACCGCGCA
CGCCTCATAAATGCGTACTT

Product: DNA-cytosine methyltransferase

Products: NA

Alternate protein names: M.AgeI; Cytosine-specific methyltransferase AgeI [H]

Number of amino acids: Translated: 438; Mature: 437

Protein sequence:

>438_residues
MPQVPFSVVDLFCGAGGLSQGFRDAGFRVVAGSDNDPDAMATYRANFPEAAGITGDIRSAPIKEQLLEAARRATVLIGGP
PCQAFSQVRNHTRMIDDPRNSLYREFVDVLKQSLPPAFLIENVTGMDQMGVRDQIASDLSLDGEYTVLPQVVDAADFGVP
QTRKRLLFVGVRSRSRMSPPILMGAGATDAFALERVSAGGRARYRVVFKESAWGERASAILADPDNLSVVSAADAIGDLA
CLPLGNRQDIIPYSDLPDAQSAYQRLMRKGGKATLSNVQVPRINEDTKLRLGGIPPGGNYRDLRAGLQERYITGQRWGQD
NGSGMLSRKHFYAYRRLHPDLWAWTLNTKGDAVYHYNKLRALSVREFARLQSFPDRFVFTTDQRRGKIEGRHDGGPAHSR
YRQVGNAVPPLLARAVAESLIAQLKLLDFTDRSLVAAE

Sequences:

>Translated_438_residues
MPQVPFSVVDLFCGAGGLSQGFRDAGFRVVAGSDNDPDAMATYRANFPEAAGITGDIRSAPIKEQLLEAARRATVLIGGP
PCQAFSQVRNHTRMIDDPRNSLYREFVDVLKQSLPPAFLIENVTGMDQMGVRDQIASDLSLDGEYTVLPQVVDAADFGVP
QTRKRLLFVGVRSRSRMSPPILMGAGATDAFALERVSAGGRARYRVVFKESAWGERASAILADPDNLSVVSAADAIGDLA
CLPLGNRQDIIPYSDLPDAQSAYQRLMRKGGKATLSNVQVPRINEDTKLRLGGIPPGGNYRDLRAGLQERYITGQRWGQD
NGSGMLSRKHFYAYRRLHPDLWAWTLNTKGDAVYHYNKLRALSVREFARLQSFPDRFVFTTDQRRGKIEGRHDGGPAHSR
YRQVGNAVPPLLARAVAESLIAQLKLLDFTDRSLVAAE
>Mature_437_residues
PQVPFSVVDLFCGAGGLSQGFRDAGFRVVAGSDNDPDAMATYRANFPEAAGITGDIRSAPIKEQLLEAARRATVLIGGPP
CQAFSQVRNHTRMIDDPRNSLYREFVDVLKQSLPPAFLIENVTGMDQMGVRDQIASDLSLDGEYTVLPQVVDAADFGVPQ
TRKRLLFVGVRSRSRMSPPILMGAGATDAFALERVSAGGRARYRVVFKESAWGERASAILADPDNLSVVSAADAIGDLAC
LPLGNRQDIIPYSDLPDAQSAYQRLMRKGGKATLSNVQVPRINEDTKLRLGGIPPGGNYRDLRAGLQERYITGQRWGQDN
GSGMLSRKHFYAYRRLHPDLWAWTLNTKGDAVYHYNKLRALSVREFARLQSFPDRFVFTTDQRRGKIEGRHDGGPAHSRY
RQVGNAVPPLLARAVAESLIAQLKLLDFTDRSLVAAE

Specific function: This methylase recognizes the double-stranded sequence ACCGGT, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the AgeI endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1788271, Length=206, Percent_Identity=28.1553398058252, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 48160; Mature: 48029

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: PS00095 C5_MTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQVPFSVVDLFCGAGGLSQGFRDAGFRVVAGSDNDPDAMATYRANFPEAAGITGDIRSA
CCCCCHHHHHHHHCCCCHHCCHHHCCEEEEECCCCCCCHHEEEECCCCCCCCCCCCCCCC
PIKEQLLEAARRATVLIGGPPCQAFSQVRNHTRMIDDPRNSLYREFVDVLKQSLPPAFLI
CHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEH
ENVTGMDQMGVRDQIASDLSLDGEYTVLPQVVDAADFGVPQTRKRLLFVGVRSRSRMSPP
HCCCCCHHCCHHHHHHHHCCCCCCEEEHHHHHHHHHCCCCHHCCEEEEEEECCCCCCCCC
ILMGAGATDAFALERVSAGGRARYRVVFKESAWGERASAILADPDNLSVVSAADAIGDLA
EEEECCCCHHHHHHHHCCCCCEEEEEEEECCCCCCCCCEEEECCCCCEEEEHHHHHHCEE
CLPLGNRQDIIPYSDLPDAQSAYQRLMRKGGKATLSNVQVPRINEDTKLRLGGIPPGGNY
EECCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEECCCCCCCCEEEECCCCCCCCH
RDLRAGLQERYITGQRWGQDNGSGMLSRKHFYAYRRLHPDLWAWTLNTKGDAVYHYNKLR
HHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCEEEEECHHH
ALSVREFARLQSFPDRFVFTTDQRRGKIEGRHDGGPAHSRYRQVGNAVPPLLARAVAESL
HHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHH
IAQLKLLDFTDRSLVAAE
HHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
PQVPFSVVDLFCGAGGLSQGFRDAGFRVVAGSDNDPDAMATYRANFPEAAGITGDIRSA
CCCCHHHHHHHHCCCCHHCCHHHCCEEEEECCCCCCCHHEEEECCCCCCCCCCCCCCCC
PIKEQLLEAARRATVLIGGPPCQAFSQVRNHTRMIDDPRNSLYREFVDVLKQSLPPAFLI
CHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEH
ENVTGMDQMGVRDQIASDLSLDGEYTVLPQVVDAADFGVPQTRKRLLFVGVRSRSRMSPP
HCCCCCHHCCHHHHHHHHCCCCCCEEEHHHHHHHHHCCCCHHCCEEEEEEECCCCCCCCC
ILMGAGATDAFALERVSAGGRARYRVVFKESAWGERASAILADPDNLSVVSAADAIGDLA
EEEECCCCHHHHHHHHCCCCCEEEEEEEECCCCCCCCCEEEECCCCCEEEEHHHHHHCEE
CLPLGNRQDIIPYSDLPDAQSAYQRLMRKGGKATLSNVQVPRINEDTKLRLGGIPPGGNY
EECCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEECCCCCCCCEEEECCCCCCCCH
RDLRAGLQERYITGQRWGQDNGSGMLSRKHFYAYRRLHPDLWAWTLNTKGDAVYHYNKLR
HHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCEEEEECHHH
ALSVREFARLQSFPDRFVFTTDQRRGKIEGRHDGGPAHSRYRQVGNAVPPLLARAVAESL
HHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHH
IAQLKLLDFTDRSLVAAE
HHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8901102 [H]