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The map label for this gene is rutB [H]

Identifier: 86750229

GI number: 86750229

Start: 3550901

End: 3551575

Strand: Reverse

Name: rutB [H]

Synonym: RPB_3116

Alternate gene names: 86750229

Gene position: 3551575-3550901 (Counterclockwise)

Preceding gene: 86750230

Following gene: 86750228

Centisome position: 66.61

GC content: 67.85

Gene sequence:

>675_bases
TTGCCGTCGTCCACAGCGCTTCTTCGCAATCTCGCCGACAAGACCGATCCGCGGCATGCGGCGCTGCTGGTGATCGACGT
GCAGAACCATTTCGCCGCGCCCGGCGGTTTCTTCGACCGGATCGGCGCCGACCTCGGCGTCGTGCAGCGGGAGCGGGTCC
CCAATCTGTTGCGGCTGATCTACGCCGCGCGCCGGGCCGGCGTTCTGGTGATCTTCGTCCAGGCGATCTACGATCCCGAG
CATCTGTCGGATGCGATGCGCGAACGCAACGCGCGGATCGGCAGCGAGTTGCCGCGCTGCCGCAGCGGCACATGGGGCGC
CGAGTTCTACCGCGTCGCACCGGAGCCGGGCGAGCCGGTCGTGATCAAGCATCGCTACAGCGCGATGGTCGGGCCGCAAT
TGCCCGAGCTGTTGCGCGATCGCGGCATCCGGAGCCTGCTGCTCACGGGCATCGCGACCGACACGTGTGTCGAATCCGCC
GGGCGCGACGCGTATTTCCGCGACTACTACGTGACGCTGGTCGGCGATTGCTGCGGCGCCGCCAGCGACGAGGATCATCG
CGGTGCGCTGAAGCGGTTCCATCGCGACTACGGAGCGGTGGTCGATGCCGACGACGTGATCGCCACCTGGCAAGGCGTCG
GGGCGACCGCTGCCGTGGCGAATTCGGCCTTATGA

Upstream 100 bases:

>100_bases
CAAAACGTCTTGACTAACTGGTCAGTATCTTGAATGTTTCGCGGCGGGTGAGTGGACCGGCGGTCGTGCCGGTCGATGCC
CTCGCATTCCGGAGCCCTGC

Downstream 100 bases:

>100_bases
ATCTGTATCGTGACCTTCTCGCGCCCGGCCTGTTCCGGATGTCGCCGGATCGCAGCCACGCGATGGCGCATGCGGCGCTG
CGCCGCGCCGTGCCGTGGCG

Product: isochorismatase hydrolase

Products: NA

Alternate protein names: Ureidoacrylate amidohydrolase [H]

Number of amino acids: Translated: 224; Mature: 223

Protein sequence:

>224_residues
MPSSTALLRNLADKTDPRHAALLVIDVQNHFAAPGGFFDRIGADLGVVQRERVPNLLRLIYAARRAGVLVIFVQAIYDPE
HLSDAMRERNARIGSELPRCRSGTWGAEFYRVAPEPGEPVVIKHRYSAMVGPQLPELLRDRGIRSLLLTGIATDTCVESA
GRDAYFRDYYVTLVGDCCGAASDEDHRGALKRFHRDYGAVVDADDVIATWQGVGATAAVANSAL

Sequences:

>Translated_224_residues
MPSSTALLRNLADKTDPRHAALLVIDVQNHFAAPGGFFDRIGADLGVVQRERVPNLLRLIYAARRAGVLVIFVQAIYDPE
HLSDAMRERNARIGSELPRCRSGTWGAEFYRVAPEPGEPVVIKHRYSAMVGPQLPELLRDRGIRSLLLTGIATDTCVESA
GRDAYFRDYYVTLVGDCCGAASDEDHRGALKRFHRDYGAVVDADDVIATWQGVGATAAVANSAL
>Mature_223_residues
PSSTALLRNLADKTDPRHAALLVIDVQNHFAAPGGFFDRIGADLGVVQRERVPNLLRLIYAARRAGVLVIFVQAIYDPEH
LSDAMRERNARIGSELPRCRSGTWGAEFYRVAPEPGEPVVIKHRYSAMVGPQLPELLRDRGIRSLLLTGIATDTCVESAG
RDAYFRDYYVTLVGDCCGAASDEDHRGALKRFHRDYGAVVDADDVIATWQGVGATAAVANSAL

Specific function: In vivo, quickly hydrolyzes the ureidoacrylate peracid to avoid toxicity, but can also hydrolyzes ureidoacrylate that is formed spontaneously from ureidoacrylate peracid. One of the products of hydrolysis, carbamate, hydrolyzes spontaneously, thereby rele

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismatase family. RutB subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081820, Length=215, Percent_Identity=32.5581395348837, Blast_Score=115, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019916
- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: NA

Molecular weight: Translated: 24462; Mature: 24331

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSSTALLRNLADKTDPRHAALLVIDVQNHFAAPGGFFDRIGADLGVVQRERVPNLLRLI
CCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHH
YAARRAGVLVIFVQAIYDPEHLSDAMRERNARIGSELPRCRSGTWGAEFYRVAPEPGEPV
HHHHHCCCCEEHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCE
VIKHRYSAMVGPQLPELLRDRGIRSLLLTGIATDTCVESAGRDAYFRDYYVTLVGDCCGA
EEEEHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCC
ASDEDHRGALKRFHRDYGAVVDADDVIATWQGVGATAAVANSAL
CCCCHHHHHHHHHHHHHCCEECCHHHHHHHCCCCHHHHHHCCCC
>Mature Secondary Structure 
PSSTALLRNLADKTDPRHAALLVIDVQNHFAAPGGFFDRIGADLGVVQRERVPNLLRLI
CCHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHH
YAARRAGVLVIFVQAIYDPEHLSDAMRERNARIGSELPRCRSGTWGAEFYRVAPEPGEPV
HHHHHCCCCEEHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCE
VIKHRYSAMVGPQLPELLRDRGIRSLLLTGIATDTCVESAGRDAYFRDYYVTLVGDCCGA
EEEEHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCC
ASDEDHRGALKRFHRDYGAVVDADDVIATWQGVGATAAVANSAL
CCCCHHHHHHHHHHHHHCCEECCHHHHHHHCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA