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The map label for this gene is ntrX [H]

Identifier: 86749997

GI number: 86749997

Start: 3277776

End: 3279143

Strand: Reverse

Name: ntrX [H]

Synonym: RPB_2880

Alternate gene names: 86749997

Gene position: 3279143-3277776 (Counterclockwise)

Preceding gene: 86749998

Following gene: 86749996

Centisome position: 61.5

GC content: 66.15

Gene sequence:

>1368_bases
ATGGCGAATGACATTTTGATCGTCGATGACGAAGCGGATATCCGCGATCTCGTCGCCGGCATTCTCGAGGACGAAGGCTT
CACGTCGCGCACCGCGCGCGACAGCGATTCCGCGTTGGCGGAGATCGCGAACCGGCGTCCGAATTTGATCTTCCTCGACA
TCTGGCTGCAGGGCAGCAAGCTCGACGGCCTGCAGTTGCTCGAGCAGATCAAGAAGGAGCACGCCGAGGTGCCGGTGGTG
ATGATCTCCGGCCACGGCAACATCGAGACCGCGGTGGCGGCGATCAAACGCGGCGCCTACGACTTCATCGAGAAGCCGTT
CAAATCCGACCGCCTGCTTCTGGTCGCGACGCGTGCGCTGGAGACCTCGCGGCTGAAACGCGAGGTGCGCGAACTGAAGC
AGCAGGCGCCGTCGGCCTCGACGCTGATGGGCCGCTCGGCCTGCATGAACCAGTTGCGCCAGACCATCGAGCGCGCCGCC
AAGGCCAACAGCCGGATTCTGATCGTCGGCCCGTCGGGGGCCGGCAAGGAACTCGCGGCGCGGACGCTGCACAACGCCTC
CGGCCGCGCCGATGGCCCGTTCGTGGTGATCAACGCCGCCGCGATCACGCCGGAGCGGATGGAGGTCGAGCTGTTCGGCA
TCGAGCCGAACGGCGAGCACGCGCGCAAGGCGGGAGCGCTGGAAGAGGCCCATGGCGGCACGCTGTTCATCGACGAAATC
GCCGACATGCCGCGCGAGACCCAGAACAAGATCCTGCGGGTGCTGGTCGAGCAGACCTTCCAGCGCTCCGGCGGCACCGC
CAAGGTCAATGTCGACGTTCGCATCATCTCGTCGACGGCGCGCAATCTCGAAGAGGAGATCGCCGAGGGACGCTTCCGCG
AGGACCTGTATCACCGGCTGTCGGTGGTGCCGATCCGGGTGCCGCCGCTGTCGGAACGGCGCGAGGACATTCCCGAATTG
ATCGAGTATTTCATGGACCAGATTTCGGCAGCGACCGGCCTGCCGAAGCGGCAGATCGGGCAGGATGCGATGGCCGTGCT
GCAGTCGCATGTCTGGCCGGGCAACGTCCGTCAGCTCCGCAACAATGTCGAGCGGGTGATGATCCTCGCCGGCGGCGGCC
CGGATGCGGTGATCACCGCCGACATGCTGCCGCAGGACGTCGGCTCGATGGTGCCGACGATGCCGACCGGCAACAATGGC
GAGCACATCATGGGGCTGCCGCTGCGCGAGGCCCGCGAGGTGTTCGAGCGCGACTACCTCATCGCGCAGATCAGCCGGTT
CTCCGGCAACATCTCGCGCACCGCCGAATTCGTCGGCATGGAGCGCTCGGCGCTGCATCGCAAGCTCAAGGCGCTCGGCG
TCGGCTGA

Upstream 100 bases:

>100_bases
GACAGCCCGTGGCACCGCCTGCCGGTGAACCAAAAGCCAAGGCCGGCAACGAACCGAAGATAGAAGCGGCGAACAGCGGC
TAGAATGACAGGCTCGACAA

Downstream 100 bases:

>100_bases
TCGTTTTTCTCTGACCTCTCAACCGGAATTGCTCGATGTCGCGCATCGCCTATGTGAACGGCCGCTATCAGGAGATGCGC
GACGCCAGCGTCAGCATCGA

Product: two component, sigma54 specific, Fis family transcriptional regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 455; Mature: 454

Protein sequence:

>455_residues
MANDILIVDDEADIRDLVAGILEDEGFTSRTARDSDSALAEIANRRPNLIFLDIWLQGSKLDGLQLLEQIKKEHAEVPVV
MISGHGNIETAVAAIKRGAYDFIEKPFKSDRLLLVATRALETSRLKREVRELKQQAPSASTLMGRSACMNQLRQTIERAA
KANSRILIVGPSGAGKELAARTLHNASGRADGPFVVINAAAITPERMEVELFGIEPNGEHARKAGALEEAHGGTLFIDEI
ADMPRETQNKILRVLVEQTFQRSGGTAKVNVDVRIISSTARNLEEEIAEGRFREDLYHRLSVVPIRVPPLSERREDIPEL
IEYFMDQISAATGLPKRQIGQDAMAVLQSHVWPGNVRQLRNNVERVMILAGGGPDAVITADMLPQDVGSMVPTMPTGNNG
EHIMGLPLREAREVFERDYLIAQISRFSGNISRTAEFVGMERSALHRKLKALGVG

Sequences:

>Translated_455_residues
MANDILIVDDEADIRDLVAGILEDEGFTSRTARDSDSALAEIANRRPNLIFLDIWLQGSKLDGLQLLEQIKKEHAEVPVV
MISGHGNIETAVAAIKRGAYDFIEKPFKSDRLLLVATRALETSRLKREVRELKQQAPSASTLMGRSACMNQLRQTIERAA
KANSRILIVGPSGAGKELAARTLHNASGRADGPFVVINAAAITPERMEVELFGIEPNGEHARKAGALEEAHGGTLFIDEI
ADMPRETQNKILRVLVEQTFQRSGGTAKVNVDVRIISSTARNLEEEIAEGRFREDLYHRLSVVPIRVPPLSERREDIPEL
IEYFMDQISAATGLPKRQIGQDAMAVLQSHVWPGNVRQLRNNVERVMILAGGGPDAVITADMLPQDVGSMVPTMPTGNNG
EHIMGLPLREAREVFERDYLIAQISRFSGNISRTAEFVGMERSALHRKLKALGVG
>Mature_454_residues
ANDILIVDDEADIRDLVAGILEDEGFTSRTARDSDSALAEIANRRPNLIFLDIWLQGSKLDGLQLLEQIKKEHAEVPVVM
ISGHGNIETAVAAIKRGAYDFIEKPFKSDRLLLVATRALETSRLKREVRELKQQAPSASTLMGRSACMNQLRQTIERAAK
ANSRILIVGPSGAGKELAARTLHNASGRADGPFVVINAAAITPERMEVELFGIEPNGEHARKAGALEEAHGGTLFIDEIA
DMPRETQNKILRVLVEQTFQRSGGTAKVNVDVRIISSTARNLEEEIAEGRFREDLYHRLSVVPIRVPPLSERREDIPELI
EYFMDQISAATGLPKRQIGQDAMAVLQSHVWPGNVRQLRNNVERVMILAGGGPDAVITADMLPQDVGSMVPTMPTGNNGE
HIMGLPLREAREVFERDYLIAQISRFSGNISRTAEFVGMERSALHRKLKALGVG

Specific function: Member of the two-component regulatory system ntrY/ntrX involved in the activation of nitrogen assimilatory genes such as glnA. NtrX is probably phosphorylated by ntrY and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=469, Percent_Identity=35.6076759061834, Blast_Score=294, Evalue=1e-80,
Organism=Escherichia coli, GI1788550, Length=465, Percent_Identity=36.1290322580645, Blast_Score=289, Evalue=3e-79,
Organism=Escherichia coli, GI1790437, Length=453, Percent_Identity=33.9955849889625, Blast_Score=248, Evalue=8e-67,
Organism=Escherichia coli, GI1788905, Length=459, Percent_Identity=34.640522875817, Blast_Score=243, Evalue=2e-65,
Organism=Escherichia coli, GI1786524, Length=328, Percent_Identity=37.8048780487805, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI1789233, Length=255, Percent_Identity=41.9607843137255, Blast_Score=199, Evalue=4e-52,
Organism=Escherichia coli, GI1787583, Length=337, Percent_Identity=35.3115727002967, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI87082152, Length=331, Percent_Identity=36.5558912386707, Blast_Score=192, Evalue=5e-50,
Organism=Escherichia coli, GI1789087, Length=246, Percent_Identity=41.869918699187, Blast_Score=189, Evalue=3e-49,
Organism=Escherichia coli, GI87082117, Length=255, Percent_Identity=40.3921568627451, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI87081872, Length=330, Percent_Identity=33.3333333333333, Blast_Score=161, Evalue=6e-41,
Organism=Escherichia coli, GI1789828, Length=263, Percent_Identity=31.9391634980989, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI87081858, Length=297, Percent_Identity=27.6094276094276, Blast_Score=118, Evalue=6e-28,
Organism=Escherichia coli, GI1786599, Length=115, Percent_Identity=40, Blast_Score=84, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 50093; Mature: 49962

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANDILIVDDEADIRDLVAGILEDEGFTSRTARDSDSALAEIANRRPNLIFLDIWLQGSK
CCCCEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEEEEEECCC
LDGLQLLEQIKKEHAEVPVVMISGHGNIETAVAAIKRGAYDFIEKPFKSDRLLLVATRAL
CCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCHHHHHCCCCCCCEEEEEHHHH
ETSRLKREVRELKQQAPSASTLMGRSACMNQLRQTIERAAKANSRILIVGPSGAGKELAA
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHH
RTLHNASGRADGPFVVINAAAITPERMEVELFGIEPNGEHARKAGALEEAHGGTLFIDEI
HHHHCCCCCCCCCEEEEEEEECCCCCEEEEEEEECCCCHHHHHHCCHHHCCCCEEEEHHH
ADMPRETQNKILRVLVEQTFQRSGGTAKVNVDVRIISSTARNLEEEIAEGRFREDLYHRL
HCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEECHHHHHHHHHHHCCHHHHHHHHHH
SVVPIRVPPLSERREDIPELIEYFMDQISAATGLPKRQIGQDAMAVLQSHVWPGNVRQLR
EEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCHHHHHHHHHCCCCCHHHHHH
NNVERVMILAGGGPDAVITADMLPQDVGSMVPTMPTGNNGEHIMGLPLREAREVFERDYL
CCCEEEEEEECCCCCCEEEHHHCHHHHHHHCCCCCCCCCCCEEECCCHHHHHHHHHHHHH
IAQISRFSGNISRTAEFVGMERSALHRKLKALGVG
HHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ANDILIVDDEADIRDLVAGILEDEGFTSRTARDSDSALAEIANRRPNLIFLDIWLQGSK
CCCEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEEEEEECCC
LDGLQLLEQIKKEHAEVPVVMISGHGNIETAVAAIKRGAYDFIEKPFKSDRLLLVATRAL
CCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCHHHHHCCCCCCCEEEEEHHHH
ETSRLKREVRELKQQAPSASTLMGRSACMNQLRQTIERAAKANSRILIVGPSGAGKELAA
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHH
RTLHNASGRADGPFVVINAAAITPERMEVELFGIEPNGEHARKAGALEEAHGGTLFIDEI
HHHHCCCCCCCCCEEEEEEEECCCCCEEEEEEEECCCCHHHHHHCCHHHCCCCEEEEHHH
ADMPRETQNKILRVLVEQTFQRSGGTAKVNVDVRIISSTARNLEEEIAEGRFREDLYHRL
HCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEECHHHHHHHHHHHCCHHHHHHHHHH
SVVPIRVPPLSERREDIPELIEYFMDQISAATGLPKRQIGQDAMAVLQSHVWPGNVRQLR
EEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCHHHHHHHHHCCCCCHHHHHH
NNVERVMILAGGGPDAVITADMLPQDVGSMVPTMPTGNNGEHIMGLPLREAREVFERDYL
CCCEEEEEEECCCCCCEEEHHHCHHHHHHHCCCCCCCCCCCEEECCCHHHHHHHHHHHHH
IAQISRFSGNISRTAEFVGMERSALHRKLKALGVG
HHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1661370 [H]