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The map label for this gene is eno

Identifier: 86749895

GI number: 86749895

Start: 3165621

End: 3166904

Strand: Reverse

Name: eno

Synonym: RPB_2778

Alternate gene names: 86749895

Gene position: 3166904-3165621 (Counterclockwise)

Preceding gene: 86749899

Following gene: 86749894

Centisome position: 59.4

GC content: 64.88

Gene sequence:

>1284_bases
ATGACCGCCATCGTCGACATCATCGGCCGCGAAATTCTCGACAGCCGCGGCAACCCGACCGTCGAGGTCGATGTCGTGCT
GGAGGACGGCTCGGTCGGCCGCGCGGCGGTGCCGTCCGGGGCCTCCACCGGCGCCCACGAGGCGGTCGAGCTGCGCGACG
GCGACAAAAGCCGCTATCTCGGCAAGGGCGTGCTGAAGGCGGTCGAGGCGGTCAATGGCGAATTGTTCGACGCGCTCGGC
GGCATGGATGCGGAAGCGCAGGTCCAGATCGACCAGACCATGATCGAGCTCGACGGCACCCCGAACAAGAGCCGACTCGG
CGCCAACGCCATCCTCGGCGTCTCGCTCGCCGTCGCCAAGGCGGCGGCGTCGTCCTACGACCTGCCGCTTTATCGCTATG
TCGGCGGCACCTCGGCGCGGACGCTGCCGGTGCCGATGATGAACATCATCAACGGTGGCGCTCATGCCGACAATCCGATC
GATTTCCAGGAATTCATGATCATGCCGGCCGGTGCCGCGAGCTTCAGCGAAGCGCTGCGCTGCGGTTCGGAAATCTTTCA
CACGCTGAAGGGCGAACTGAAGAAGGCCGGCCACAACACCAATGTCGGCGACGAGGGCGGCTTCGCGCCGAACCTGCCGT
CGGCCGATGCCGCTCTGGATTTCGTGGTCTCGGCGATCGGCAAGGCCGGCTACAAGGCCGGCGAAGACGTGATGATCGCG
CTCGATCCGGCGTCGACCGAGTTCTTCAAGAACGGCAAATACGTCTATGAAGCGGAAGGCAGGAGCCTCGGCCCGCAGGA
GCAGGCGAAGTATCTGGCCGATCTCGTGGCGCGCTATCCGATCGTGTCGATCGAGGACGGTATGGCCGAAGACGACATCG
AAGGTTGGAAGGCGATCACCGGCCTGATCGGCGACAAGTGCCAGCTGGTCGGCGACGACCTGTTCGTCACCAACGTCACG
CGGCTGTATGACGGCATCAAGAACGGCTACGCCAACTCGATCCTGATCAAGGTCAACCAGATCGGCACGCTGACCGAAAC
GCTCGCCGCAGTCGAGATGGCGCACAAGGCCGGCTACACCGCAGTGATGTCGCATCGCTCGGGCGAGACCGAGGACTCGA
CGATTGCCGATCTCGCGGTCGCCACCAATTGCGGGCAGATCAAGACCGGCTCGCTGGCGCGCGCCGACCGCACCTCGAAG
TACAACCAGCTGCTGCGCATCGAGCAGGAGCTCGGCACCCAGGCGCATTTCGCCGGCAAGGCGGCGCTCAAGGCGTTTCG
CTGA

Upstream 100 bases:

>100_bases
GTCACCCGCCCGTCATCTCCGCGACGGCCTTTTCCGCCCCGAAGCCATGCTGTAGAAGCGGCGCAATCCTTGTCCCGTAG
AAATCCGCAGTGAGGCTCCC

Downstream 100 bases:

>100_bases
GCGCGAACTCCGCCATTGCGAGCCAACGGGGCGGCGCGAAGCGCCGCCCGATGACCGGCTCCGCGAAGCGATCCATCCTT
AGGCTGGATGTCAGAACTGG

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MTAIVDIIGREILDSRGNPTVEVDVVLEDGSVGRAAVPSGASTGAHEAVELRDGDKSRYLGKGVLKAVEAVNGELFDALG
GMDAEAQVQIDQTMIELDGTPNKSRLGANAILGVSLAVAKAAASSYDLPLYRYVGGTSARTLPVPMMNIINGGAHADNPI
DFQEFMIMPAGAASFSEALRCGSEIFHTLKGELKKAGHNTNVGDEGGFAPNLPSADAALDFVVSAIGKAGYKAGEDVMIA
LDPASTEFFKNGKYVYEAEGRSLGPQEQAKYLADLVARYPIVSIEDGMAEDDIEGWKAITGLIGDKCQLVGDDLFVTNVT
RLYDGIKNGYANSILIKVNQIGTLTETLAAVEMAHKAGYTAVMSHRSGETEDSTIADLAVATNCGQIKTGSLARADRTSK
YNQLLRIEQELGTQAHFAGKAALKAFR

Sequences:

>Translated_427_residues
MTAIVDIIGREILDSRGNPTVEVDVVLEDGSVGRAAVPSGASTGAHEAVELRDGDKSRYLGKGVLKAVEAVNGELFDALG
GMDAEAQVQIDQTMIELDGTPNKSRLGANAILGVSLAVAKAAASSYDLPLYRYVGGTSARTLPVPMMNIINGGAHADNPI
DFQEFMIMPAGAASFSEALRCGSEIFHTLKGELKKAGHNTNVGDEGGFAPNLPSADAALDFVVSAIGKAGYKAGEDVMIA
LDPASTEFFKNGKYVYEAEGRSLGPQEQAKYLADLVARYPIVSIEDGMAEDDIEGWKAITGLIGDKCQLVGDDLFVTNVT
RLYDGIKNGYANSILIKVNQIGTLTETLAAVEMAHKAGYTAVMSHRSGETEDSTIADLAVATNCGQIKTGSLARADRTSK
YNQLLRIEQELGTQAHFAGKAALKAFR
>Mature_426_residues
TAIVDIIGREILDSRGNPTVEVDVVLEDGSVGRAAVPSGASTGAHEAVELRDGDKSRYLGKGVLKAVEAVNGELFDALGG
MDAEAQVQIDQTMIELDGTPNKSRLGANAILGVSLAVAKAAASSYDLPLYRYVGGTSARTLPVPMMNIINGGAHADNPID
FQEFMIMPAGAASFSEALRCGSEIFHTLKGELKKAGHNTNVGDEGGFAPNLPSADAALDFVVSAIGKAGYKAGEDVMIAL
DPASTEFFKNGKYVYEAEGRSLGPQEQAKYLADLVARYPIVSIEDGMAEDDIEGWKAITGLIGDKCQLVGDDLFVTNVTR
LYDGIKNGYANSILIKVNQIGTLTETLAAVEMAHKAGYTAVMSHRSGETEDSTIADLAVATNCGQIKTGSLARADRTSKY
NQLLRIEQELGTQAHFAGKAALKAFR

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI5803011, Length=433, Percent_Identity=52.1939953810624, Blast_Score=420, Evalue=1e-117,
Organism=Homo sapiens, GI4503571, Length=430, Percent_Identity=51.6279069767442, Blast_Score=416, Evalue=1e-116,
Organism=Homo sapiens, GI301897477, Length=430, Percent_Identity=51.3953488372093, Blast_Score=407, Evalue=1e-114,
Organism=Homo sapiens, GI301897469, Length=430, Percent_Identity=51.3953488372093, Blast_Score=407, Evalue=1e-114,
Organism=Homo sapiens, GI301897479, Length=428, Percent_Identity=46.2616822429907, Blast_Score=353, Evalue=2e-97,
Organism=Homo sapiens, GI169201331, Length=336, Percent_Identity=24.1071428571429, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI169201757, Length=336, Percent_Identity=24.1071428571429, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI239744207, Length=336, Percent_Identity=24.1071428571429, Blast_Score=94, Evalue=3e-19,
Organism=Escherichia coli, GI1789141, Length=429, Percent_Identity=61.0722610722611, Blast_Score=499, Evalue=1e-142,
Organism=Caenorhabditis elegans, GI17536383, Length=429, Percent_Identity=51.981351981352, Blast_Score=407, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI71995829, Length=429, Percent_Identity=51.981351981352, Blast_Score=407, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI32563855, Length=189, Percent_Identity=43.9153439153439, Blast_Score=161, Evalue=5e-40,
Organism=Saccharomyces cerevisiae, GI6321693, Length=434, Percent_Identity=50.6912442396313, Blast_Score=390, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6323985, Length=432, Percent_Identity=49.537037037037, Blast_Score=387, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6324974, Length=432, Percent_Identity=49.3055555555556, Blast_Score=386, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6324969, Length=432, Percent_Identity=49.3055555555556, Blast_Score=386, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6321968, Length=433, Percent_Identity=49.4226327944573, Blast_Score=367, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24580918, Length=431, Percent_Identity=50.8120649651972, Blast_Score=383, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580916, Length=431, Percent_Identity=50.8120649651972, Blast_Score=383, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580920, Length=431, Percent_Identity=50.8120649651972, Blast_Score=383, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580914, Length=431, Percent_Identity=50.8120649651972, Blast_Score=383, Evalue=1e-106,
Organism=Drosophila melanogaster, GI281360527, Length=431, Percent_Identity=50.8120649651972, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI17137654, Length=431, Percent_Identity=50.8120649651972, Blast_Score=382, Evalue=1e-106,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_RHOP2 (Q2IWD0)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_486391.1
- HSSP:   P0A6P9
- ProteinModelPortal:   Q2IWD0
- SMR:   Q2IWD0
- STRING:   Q2IWD0
- GeneID:   3910571
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_2778
- eggNOG:   COG0148
- HOGENOM:   HBG726599
- OMA:   DIAVGTN
- ProtClustDB:   PRK00077
- BioCyc:   RPAL316058:RPB_2778-MONOMER
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 45053; Mature: 44922

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 205-205 ACT_SITE 337-337 BINDING 155-155 BINDING 164-164 BINDING 285-285 BINDING 312-312 BINDING 337-337 BINDING 388-388

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAIVDIIGREILDSRGNPTVEVDVVLEDGSVGRAAVPSGASTGAHEAVELRDGDKSRYL
CCHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCHHHH
GKGVLKAVEAVNGELFDALGGMDAEAQVQIDQTMIELDGTPNKSRLGANAILGVSLAVAK
HHHHHHHHHHHCCHHHHHHCCCCCCEEEEEEEEEEEECCCCCHHHCCCHHHHHHHHHHHH
AAASSYDLPLYRYVGGTSARTLPVPMMNIINGGAHADNPIDFQEFMIMPAGAASFSEALR
HHHCCCCCCCEEECCCCCCCCCCCCHHHHHCCCCCCCCCCCHHHHEEECCCHHHHHHHHH
CGSEIFHTLKGELKKAGHNTNVGDEGGFAPNLPSADAALDFVVSAIGKAGYKAGEDVMIA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEE
LDPASTEFFKNGKYVYEAEGRSLGPQEQAKYLADLVARYPIVSIEDGMAEDDIEGWKAIT
ECCCCHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHH
GLIGDKCQLVGDDLFVTNVTRLYDGIKNGYANSILIKVNQIGTLTETLAAVEMAHKAGYT
HHHCCCEEEECCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCC
AVMSHRSGETEDSTIADLAVATNCGQIKTGSLARADRTSKYNQLLRIEQELGTQAHFAGK
EEEECCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHH
AALKAFR
HHHHHCC
>Mature Secondary Structure 
TAIVDIIGREILDSRGNPTVEVDVVLEDGSVGRAAVPSGASTGAHEAVELRDGDKSRYL
CHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCHHHH
GKGVLKAVEAVNGELFDALGGMDAEAQVQIDQTMIELDGTPNKSRLGANAILGVSLAVAK
HHHHHHHHHHHCCHHHHHHCCCCCCEEEEEEEEEEEECCCCCHHHCCCHHHHHHHHHHHH
AAASSYDLPLYRYVGGTSARTLPVPMMNIINGGAHADNPIDFQEFMIMPAGAASFSEALR
HHHCCCCCCCEEECCCCCCCCCCCCHHHHHCCCCCCCCCCCHHHHEEECCCHHHHHHHHH
CGSEIFHTLKGELKKAGHNTNVGDEGGFAPNLPSADAALDFVVSAIGKAGYKAGEDVMIA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEE
LDPASTEFFKNGKYVYEAEGRSLGPQEQAKYLADLVARYPIVSIEDGMAEDDIEGWKAIT
ECCCCHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHH
GLIGDKCQLVGDDLFVTNVTRLYDGIKNGYANSILIKVNQIGTLTETLAAVEMAHKAGYT
HHHCCCEEEECCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCC
AVMSHRSGETEDSTIADLAVATNCGQIKTGSLARADRTSKYNQLLRIEQELGTQAHFAGK
EEEECCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHH
AALKAFR
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA